Vesicle-associated membrane protein 8

VAMP8
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 4WY4
Identifiers
Aliases	VAMP8 , EDB, VAMP-8, vesicle associated membrane protein 8
External IDs	OMIM: 603177; MGI: 1336882; HomoloGene: 37846; GeneCards: VAMP8; OMA:VAMP8 - orthologs
Gene location (Human) Chr. Chromosome 2 (human) [1] Band 2p11.2 Start 85,561,562 bp [1] End 85,582,031 bp [1]
Gene location (Mouse) Chr. Chromosome 6 (mouse) [2] Band 6 C1|6 32.27 cM Start 72,362,206 bp [2] End 72,367,686 bp [2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in palpebral conjunctiva monocyte mucosa of transverse colon olfactory zone of nasal mucosa granulocyte jejunal mucosa rectum nasal epithelium bronchial epithelial cell skin of abdomen Top expressed in proximal tubule right kidney yolk sac granulocyte lung bone marrow human kidney urinary bladder duodenum lip More reference expression data BioGPS More reference expression data
Gene ontology Molecular function SNAP receptor activity protein binding chloride channel inhibitor activity SNARE binding Cellular component cytoplasm integral component of membrane recycling endosome vesicle cytosol endosome early endosome membrane membrane secretory granule membrane late endosome membrane plasma membrane mucin granule lysosomal membrane SNARE complex early endosome perinuclear region of cytoplasm lysosome extracellular exosome clathrin-coated vesicle membrane phagocytic vesicle membrane azurophil granule membrane specific granule membrane recycling endosome membrane tertiary granule membrane synaptic vesicle Biological process post-Golgi vesicle-mediated transport autophagy autophagosome maturation negative regulation of secretion by cell protein transport viral entry into host cell vesicle fusion eosinophil degranulation vesicle-mediated transport mucus secretion regulation of protein localization to plasma membrane positive regulation of histamine secretion by mast cell antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent autophagosome membrane docking neutrophil degranulation membrane organization regulation of molecular function transport Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 8673 22320 Ensembl ENSG00000118640 ENSMUSG00000050732 UniProt Q9BV40 O70404 RefSeq (mRNA) NM_003761 NM_016794 RefSeq (protein) NP_003752 NP_058074 Location (UCSC) Chr 2: 85.56 – 85.58 Mb Chr 6: 72.36 – 72.37 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

Vesicle-associated membrane protein 8 is a protein that in humans is encoded by the VAMP8 gene. ^{[5] [6] [7]}

Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. The protein encoded by this gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. It is associated with the perinuclear vesicular structures of the early endocytic compartment. It has been found that VAMP8 interacts specifically with the soluble NSF-attachment protein (alpha-SNAP), most likely through an VAMP8-containing SNARE complex. ^[7] Phosphorylation of VAMP8 inside the conserved SNARE-domain can suppress vesicle fusion. ^[8]

Interactions

Vesicle-associated membrane protein 8 has been shown to interact with STX4, ^{[9] [10]} SNAP23, ^{[9] [11]} STX1A, ^[12] STX8 ^[13] and STX7. ^{[13] [14]}

References

1. GRCh38: Ensembl release 89: ENSG00000118640 – Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000050732 – Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Bui TD, Wong SH, Lu L, Hong W (February 1999). "Endobrevin maps to chromosome 2 in human and chromosome 6 in mouse". Genomics. 54 (3): 579–80. doi:10.1006/geno.1998.5596. PMID   9878266.
6. Wong SH, Zhang T, Xu Y, Subramaniam VN, Griffiths G, Hong W (July 1998). "Endobrevin, a Novel Synaptobrevin/VAMP-Like Protein Preferentially Associated with the Early Endosome". Mol Biol Cell. 9 (6): 1549–63. doi:10.1091/mbc.9.6.1549. PMC   25382 . PMID   9614193.
7. "Entrez Gene: VAMP8 vesicle-associated membrane protein 8 (endobrevin)".
8. Malmersjö S, Di Palma S, Diao J, Lai Y, Pfuetzner RA, Wang AL, McMahon MA, Hayer A, Porteus M, Bodenmiller B, Brunger AT, Meyer T (July 2016). "Phosphorylation of residues inside the SNARE complex suppresses secretory vesicle fusion". EMBO Journal. 35 (16): 1721–1843. doi:10.15252/embj.201694071. PMC   5010044 . PMID   27402227.
9. Paumet F, Le Mao J, Martin S, Galli T, David B, Blank U, Roa M (June 2000). "Soluble NSF attachment protein receptors (SNAREs) in RBL-2H3 mast cells: functional role of syntaxin 4 in exocytosis and identification of a vesicle-associated membrane protein 8-containing secretory compartment". J. Immunol. 164 (11): 5850–7. doi: 10.4049/jimmunol.164.11.5850 . ISSN   0022-1767. PMID   10820264.
10. Polgár J, Chung Sul-Hee, Reed Guy L (August 2002). "Vesicle-associated membrane protein 3 (VAMP-3) and VAMP-8 are present in human platelets and are required for granule secretion". Blood. 100 (3): 1081–3. doi:10.1182/blood.V100.3.1081. ISSN   0006-4971. PMID   12130530. S2CID   36597939.
11. Imai A, Nashida Tomoko, Yoshie Sumio, Shimomura Hiromi (August 2003). "Intracellular localisation of SNARE proteins in rat parotid acinar cells: SNARE complexes on the apical plasma membrane". Arch. Oral Biol. 48 (8): 597–604. doi:10.1016/S0003-9969(03)00116-X. ISSN   0003-9969. PMID   12828989.
12. Nagamatsu S, Nakamichi Y, Watanabe T, Matsushima S, Yamaguchi S, Ni J, Itagaki E, Ishida H (January 2001). "Localization of cellubrevin-related peptide, endobrevin, in the early endosome in pancreatic beta cells and its physiological function in exo-endocytosis of secretory granules". J. Cell Sci. 114 (Pt 1): 219–227. doi:10.1242/jcs.114.1.219. ISSN   0021-9533. PMID   11112705.
13. Antonin W, Holroyd C, Fasshauer D, Pabst S, Von Mollard G F, Jahn R (Dec 2000). "A SNARE complex mediating fusion of late endosomes defines conserved properties of SNARE structure and function". EMBO J. 19 (23): 6453–64. doi:10.1093/emboj/19.23.6453. ISSN   0261-4189. PMC   305878 . PMID   11101518.
14. Wade N, Bryant N J, Connolly L M, Simpson R J, Luzio J P, Piper R C, James D E (June 2001). "Syntaxin 7 complexes with mouse Vps10p tail interactor 1b, syntaxin 6, vesicle-associated membrane protein (VAMP)8, and VAMP7 in b16 melanoma cells". J. Biol. Chem. 276 (23): 19820–7. doi: 10.1074/jbc.M010838200 . ISSN   0021-9258. PMID   11278762.

Further reading

• Bonaldo MF, Lennon G, Soares MB (1997). "Normalization and subtraction: two approaches to facilitate gene discovery". Genome Res. 6 (9): 791–806. doi: 10.1101/gr.6.9.791 . PMID   8889548.
• Fasshauer D, Antonin W, Margittai M, et al. (1999). "Mixed and non-cognate SNARE complexes. Characterization of assembly and biophysical properties". J. Biol. Chem. 274 (22): 15440–6. doi: 10.1074/jbc.274.22.15440 . PMID   10336434.
• Kim PK, Hollerbach C, Trimble WS, et al. (2000). "Identification of the endoplasmic reticulum targeting signal in vesicle-associated membrane proteins". J. Biol. Chem. 274 (52): 36876–82. doi: 10.1074/jbc.274.52.36876 . PMID   10601239.
• Paumet F, Le Mao J, Martin S, et al. (2000). "Soluble NSF attachment protein receptors (SNAREs) in RBL-2H3 mast cells: functional role of syntaxin 4 in exocytosis and identification of a vesicle-associated membrane protein 8-containing secretory compartment". J. Immunol. 164 (11): 5850–7. doi: 10.4049/jimmunol.164.11.5850 . PMID   10820264.
• Antonin W, Holroyd C, Tikkanen R, et al. (2000). "The R-SNARE Endobrevin/VAMP-8 Mediates Homotypic Fusion of Early Endosomes and Late Endosomes". Mol. Biol. Cell. 11 (10): 3289–98. doi:10.1091/mbc.11.10.3289. PMC   14992 . PMID   11029036.
• Antonin W, Holroyd C, Fasshauer D, et al. (2001). "A SNARE complex mediating fusion of late endosomes defines conserved properties of SNARE structure and function". EMBO J. 19 (23): 6453–64. doi:10.1093/emboj/19.23.6453. PMC   305878 . PMID   11101518.
• Nagamatsu S, Nakamichi Y, Watanabe T, et al. (2001). "Localization of cellubrevin-related peptide, endobrevin, in the early endosome in pancreatic beta cells and its physiological function in exo-endocytosis of secretory granules". J. Cell Sci. 114 (Pt 1): 219–227. doi:10.1242/jcs.114.1.219. PMID   11112705.
• Wade N, Bryant NJ, Connolly LM, et al. (2001). "Syntaxin 7 complexes with mouse Vps10p tail interactor 1b, syntaxin 6, vesicle-associated membrane protein (VAMP)8, and VAMP7 in b16 melanoma cells". J. Biol. Chem. 276 (23): 19820–7. doi: 10.1074/jbc.M010838200 . PMID   11278762.
• Antonin W, Fasshauer D, Becker S, et al. (2002). "Crystal structure of the endosomal SNARE complex reveals common structural principles of all SNAREs". Nat. Struct. Biol. 9 (2): 107–11. doi:10.1038/nsb746. hdl: 11858/00-001M-0000-0012-F459-F . PMID   11786915. S2CID   17724790.
• Polgár J, Chung SH, Reed GL (2002). "Vesicle-associated membrane protein 3 (VAMP-3) and VAMP-8 are present in human platelets and are required for granule secretion". Blood. 100 (3): 1081–3. doi:10.1182/blood.V100.3.1081. PMID   12130530. S2CID   36597939.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi: 10.1073/pnas.242603899 . PMC   139241 . PMID   12477932.
• Imai A, Nashida T, Yoshie S, Shimomura H (2003). "Intracellular localisation of SNARE proteins in rat parotid acinar cells: SNARE complexes on the apical plasma membrane". Arch. Oral Biol. 48 (8): 597–604. doi:10.1016/S0003-9969(03)00116-X. PMID   12828989.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC   528928 . PMID   15489334.
• Hillier LW, Graves TA, Fulton RS, et al. (2005). "Generation and annotation of the DNA sequences of human chromosomes 2 and 4". Nature. 434 (7034): 724–31. Bibcode:2005Natur.434..724H. doi: 10.1038/nature03466 . PMID   15815621.
• Brinkman JF, Ottenheim CP, de Jong LA, et al. (2006). "VAMP5 and VAMP8 are most likely not involved in primary open-angle glaucoma". Mol. Vis. 11: 582–6. PMID   16110299.
• Oishi Y, Arakawa T, Tanimura A, et al. (2007). "Role of VAMP-2, VAMP-7, and VAMP-8 in constitutive exocytosis from HSY cells". Histochem. Cell Biol. 125 (3): 273–81. doi:10.1007/s00418-005-0068-y. PMID   16195891. S2CID   10541252.
• Shiffman D, Rowland CM, Louie JZ, et al. (2006). "Gene variants of VAMP8 and HNRPUL1 are associated with early-onset myocardial infarction". Arterioscler. Thromb. Vasc. Biol. 26 (7): 1613–8. doi: 10.1161/01.ATV.0000226543.77214.e4 . PMID   16690874.