Amoeba proteus

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Amoeba proteus
Amoeba proteus with many pseudopodia.jpg
Amoeba proteus
Scientific classification
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A. proteus
Binomial name
Amoeba proteus
(Pallas, 1766) Leidy, 1878
Synonyms

Volvox proteusPallas, 1766

  • Proteus diffluensO.F. Müller, 1786
  • Chaos diffluens(O.F. Müller, 1786) Schaeffer, 1926

Amoeba proteus is a large species of amoeba closely related to another genus of giant amoebae, Chaos . As such, the species is sometimes given the alternative scientific name Chaos diffluens. [1] [2]

Contents

Amoeba proteus in locomotion Amoeba proteus locomotive form.jpg
Amoeba proteus in locomotion

This protozoan uses extensions called pseudopodia to move and to eat smaller unicellular organisms. Food is enveloped inside the cell's cytoplasm in a food vacuole, [3] where ingested matter is slowly broken down by enzymes. A. proteus inhabits freshwater environments and feeds on protozoans, algae, rotifers, and even other smaller amoebae. They are colorless, but they may have colored inclusions derived from their food. [4]

A. proteus possesses a thick-walled nucleus containing granular chromatin, and is therefore a eukaryote. Its membrane consists of a phospholipid bilayer similar to other eukaryotic organisms.[ citation needed ]

History

The first description of this amoeba is probably that of August Johann Rösel von Rosenhof who, in 1755, published drawings of an amoeboid protozoan he called the "little Proteus". [5] Subsequently, various authors assigned Rösel's organism and other amoeboid protozoa various names: Carl Linnaeus termed Rösel's organism Chaos protheus in 1758. [5] Otto Friedrich Müller referred to it as Proteus diffluens in 1786. [5] In 1878, Joseph Leidy proposed the current name Amoeba proteus to describe Rösel's Proteus, Proteus diffluens, and another described amoeba Amoeba princeps. [5] [6]

Molecular mechanisms regulating locomotion in Amoeba proteus

The locomotion of Amoeba proteus exhibits chaotic dynamics described by a low-dimensional chaotic attractor with a correlation dimension around 3-4, indicating that the seemingly random movement arises from deterministic cooperative interactions among a small number of processes like sol-gel transformations, cytoplasmic streaming, and calcium-mediated reactions. [7] The Arp2/3 complex plays a crucial role in regulating actin polymerization and organization, localizing in the cortical actin network, discrete adhesion foci, and the perinuclear region. However, actin polymerization at the leading edge of migrating pseudopodia occurs independently of the Arp2/3 complex. During migration, changes in the ratio of filamentous actin (F-actin) to total actin correlate with the contractile state of the cell cortex, where an increasing F-actin/total actin ratio corresponds to isometric contraction, while a decreasing ratio indicates isotonic contraction associated with cytoskeleton disintegration. [8] Furthermore, the Rac/PAK pathway is involved in regulating cell migration, with the PAK kinase MIHCK acting as an effector for the Rac-like protein, and inhibition of MIHCK disrupts normal migration and pseudopod formation, although it does not directly affect actin polymerization. [9]

Reproduction

Although Amoeba proteus has most of the key proteins associated with sexual processes (as do other amoebae), [10] no evidence of meiosis or sexual activity has been reported.[ citation needed ]

Amoeba proteus in motion
Amoeba engulfing a diatom

See also

Related Research Articles

<span class="mw-page-title-main">Pseudopodia</span> False leg found on slime molds, archaea, protozoans, leukocytes and certain bacteria

A pseudopod or pseudopodium is a temporary arm-like projection of a eukaryotic cell membrane that is emerged in the direction of movement. Filled with cytoplasm, pseudopodia primarily consist of actin filaments and may also contain microtubules and intermediate filaments. Pseudopods are used for motility and ingestion. They are often found in amoebas.

<span class="mw-page-title-main">Amoebozoa</span> Phylum of protozoans

Amoebozoa is a major taxonomic group containing about 2,400 described species of amoeboid protists, often possessing blunt, fingerlike, lobose pseudopods and tubular mitochondrial cristae. In traditional classification schemes, Amoebozoa is usually ranked as a phylum within either the kingdom Protista or the kingdom Protozoa. In the classification favored by the International Society of Protistologists, it is retained as an unranked "supergroup" within Eukaryota. Molecular genetic analysis supports Amoebozoa as a monophyletic clade. Modern studies of eukaryotic phylogenetic trees identify it as the sister group to Opisthokonta, another major clade which contains both fungi and animals as well as several other clades comprising some 300 species of unicellular eukaryotes. Amoebozoa and Opisthokonta are sometimes grouped together in a high-level taxon, variously named Unikonta, Amorphea or Opimoda.

<i>Naegleria</i> Genus of protists

Naegleria is a free living amoebae protist genus consisting of 47 described species often found in warm aquatic environments as well as soil habitats worldwide. It has three life cycle forms: the amoeboid stage, the cyst stage, and the flagellated stage, and has been routinely studied for its ease in change from amoeboid to flagellated stages. The Naegleria genera became famous when Naegleria fowleri, a human pathogenic strain and the causative agent of primary amoebic meningoencephalitis (PAM), was discovered in 1965. Most species in the genus, however, are nonpathogenic, meaning they do not cause disease.

<span class="mw-page-title-main">Rhizaria</span> Infrakingdom of protists

The Rhizaria are a diverse and species-rich supergroup of mostly unicellular eukaryotes. Except for the Chlorarachniophytes and three species in the genus Paulinella in the phylum Cercozoa, they are all non-photosynthethic, but many foraminifera and radiolaria have a symbiotic relationship with unicellular algae. A multicellular form, Guttulinopsis vulgaris, a cellular slime mold, has been described. This group was used by Cavalier-Smith in 2002, although the term "Rhizaria" had been long used for clades within the currently recognized taxon. Being described mainly from rDNA sequences, they vary considerably in form, having no clear morphological distinctive characters (synapomorphies), but for the most part they are amoeboids with filose, reticulose, or microtubule-supported pseudopods. In the absence of an apomorphy, the group is ill-defined, and its composition has been very fluid. Some Rhizaria possess mineral exoskeletons, which are in different clades within Rhizaria made out of opal, celestite, or calcite. Certain species can attain sizes of more than a centimeter with some species being able to form cylindrical colonies approximately 1 cm in diameter and greater than 1 m in length. They feed by capturing and engulfing prey with the extensions of their pseudopodia; forms that are symbiotic with unicellular algae contribute significantly to the total primary production of the ocean.

<span class="mw-page-title-main">Lobosa</span> Phylum of protozoans

Lobosa is a taxonomic group of amoebae in the phylum Amoebozoa. Most lobosans possess broad, bluntly rounded pseudopods, although one genus in the group, the recently discovered Sapocribrum, has slender and threadlike (filose) pseudopodia. In current classification schemes, Lobosa is a subphylum, composed mainly of amoebae that have lobose pseudopods but lack cilia or flagella.

<span class="mw-page-title-main">Contractile vacuole</span> Organelle used in regulating osmosis

A contractile vacuole (CV) is a sub-cellular structure (organelle) involved in osmoregulation. It is found predominantly in protists and in unicellular algae. It was previously known as pulsatile or pulsating vacuole.

Cell migration is a central process in the development and maintenance of multicellular organisms. Tissue formation during embryonic development, wound healing and immune responses all require the orchestrated movement of cells in particular directions to specific locations. Cells often migrate in response to specific external signals, including chemical signals and mechanical signals. Errors during this process have serious consequences, including intellectual disability, vascular disease, tumor formation and metastasis. An understanding of the mechanism by which cells migrate may lead to the development of novel therapeutic strategies for controlling, for example, invasive tumour cells.

<i>Chaos</i> (genus) Genus of microscopic organisms

Chaos is a genus of single-celled amoeboid organisms in the family Amoebidae. The largest and most-known species, the so-called "giant amoeba", can reach lengths up to 5 mm, although most specimens fall between 1 and 3 mm.

<span class="mw-page-title-main">Amoebidae</span> Family of protozoans

The Amoebidae are a family of Amoebozoa, including naked amoebae that produce multiple pseudopodia of indeterminate length. These are roughly cylindrical with granular endoplasm and no subpseudopodia, as found in other members of the class Tubulinea. During locomotion one pseudopod typically becomes dominant and the others are retracted as the body flows into it. In some cases the cell moves by "walking", with relatively permanent pseudopodia serving as limbs.

Polychaos dubium is a freshwater amoeboid and one of the larger species of single-celled eukaryote. Like other amoebozoans, P. dubium moves by means of temporary projections called pseudopods. P. dubium reportedly has one of the largest genome size of any organism known, though the authors of a 2004 study suggest treating that measurement with caution.

<i>Amoeba</i> (genus) Genus of Protozoa

Amoeba is a genus of single-celled amoeboids in the family Amoebidae. The type species of the genus is Amoeba proteus, a common freshwater organism, widely studied in classrooms and laboratories.

<span class="mw-page-title-main">Amoeboid movement</span> Mode of locomotion in eukaryotic cells

Amoeboid movement is the most typical mode of locomotion in adherent eukaryotic cells. It is a crawling-like type of movement accomplished by protrusion of cytoplasm of the cell involving the formation of pseudopodia ("false-feet") and posterior uropods. One or more pseudopodia may be produced at a time depending on the organism, but all amoeboid movement is characterized by the movement of organisms with an amorphous form that possess no set motility structures.

<span class="mw-page-title-main">Testate amoebae</span>

Testate amoebae are a polyphyletic group of unicellular amoeboid protists, which differ from naked amoebae in the presence of a test that partially encloses the cell, with an aperture from which the pseudopodia emerge, that provides the amoeba with shelter from predators and environmental conditions.

Fonticula is a genus of cellular slime mold which forms a fruiting body in a volcano shape. As long ago as 1979 it has been known to not have a close relationship with either the Dictyosteliida or the Acrasidae, the two well-established groups of cellular slime molds. In 1979, Fonticula was made a new genus of its own due to the unique characteristics of its fruiting body, with only one species: Fonticula alba.

<span class="mw-page-title-main">Arp2/3 complex</span> Macromolecular complex

Arp2/3 complex is a seven-subunit protein complex that plays a major role in the regulation of the actin cytoskeleton. It is a major component of the actin cytoskeleton and is found in most actin cytoskeleton-containing eukaryotic cells. Two of its subunits, the Actin-Related Proteins ARP2 and ARP3, closely resemble the structure of monomeric actin and serve as nucleation sites for new actin filaments. The complex binds to the sides of existing ("mother") filaments and initiates growth of a new ("daughter") filament at a distinctive 70 degree angle from the mother. Branched actin networks are created as a result of this nucleation of new filaments. The regulation of rearrangements of the actin cytoskeleton is important for processes like cell locomotion, phagocytosis, and intracellular motility of lipid vesicles.

<span class="mw-page-title-main">Vampyrellida</span> Order of single-celled organisms

The vampyrellids, colloquially known as vampire amoebae, are a group of free-living predatory amoebae classified as part of the lineage Endomyxa. They are distinguished from other groups of amoebae by their irregular cell shape with propensity to fuse and split like plasmodial organisms, and their life cycle with a digestive cyst stage that digests the gathered food. They appear worldwide in marine, brackish, freshwater and soil habitats. They are important predators of an enormous variety of microscopic organisms, from algae to fungi and animals. They are also known as aconchulinid amoebae.

An actin nucleation core is a protein trimer with three actin monomers. It is called a nucleation core because it leads to the energetically favorable elongation reaction once a tetramer is formed from a trimer. Actin protein dimers and trimers are energetically unfavorable. Actin nucleators like the Arp2/3 complex of proteins from the formin family are most frequently involved in this process. Actin nucleation factors start the polymerization of actin within cells.

<span class="mw-page-title-main">Amoeba</span> Cellular body type

An amoeba, often called an amoeboid, is a type of cell or unicellular organism with the ability to alter its shape, primarily by extending and retracting pseudopods. Amoebae do not form a single taxonomic group; instead, they are found in every major lineage of eukaryotic organisms. Amoeboid cells occur not only among the protozoa, but also in fungi, algae, and animals.

<i>Vampyrella</i> Genus of single-celled organisms

Vampyrella is a genus of amoebae belonging to the vampyrellid cercozoans usually ranging from 30-60 µm. Members of the genus alternate between two life stages: a free-living trophozoite stage and a cyst stage in which mitosis occurs. This taxon has received a great deal of attention due to their peculiar feeding behaviour of perforating the cell wall of algal cells and drawing out the contents for nourishment.

<span class="mw-page-title-main">Viridiraptoridae</span> Family of predatorial protists

Viridiraptoridae, previously known as clade X, is a clade of heterotrophic protists in the phylum Cercozoa. They're a family of glissomonads, a group containing a vast, mostly undescribed diversity of soil and freshwater organisms.

References

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  2. "Amoeba proteus (Amoeba) (Chaos diffluens)". Uniprot.org.
  3. Nishihara, Eri; Shimmen, Teruo; Sonobe, Seiji (2004). "Functional Characterization of Contractile Vacuole Isolated from Amoeba proteus". Cell Structure and Function. 29 (4): 85–90. doi: 10.1247/csf.29.85 . PMID   15665503.
  4. Rachael (8 February 2023). "Facts about Amoeba". RSScience. Retrieved 2 June 2023.
  5. 1 2 3 4 Lorch, I. Joan (1973). "Some Historical Aspects of Amoeba Studies". The Biology of Amoeba. pp. 1–36. doi:10.1016/B978-0-12-384850-5.50007-5. ISBN   978-0-12-384850-5.
  6. Leidy, Joseph (April 1878). "Amoeba Proteus". The American Naturalist. 12 (4): 235–238. doi:10.1086/272082. S2CID   222321758.
  7. Kłopocka, W.; Moraczewska, J.; Rę;dowicz, M. J. (April 2005). "Characterisation of the Rac/PAK pathway in Amoeba proteus". Protoplasma. 225 (1–2): 77–84. doi:10.1007/s00709-004-0076-0. PMID   15948264.{{cite journal}}: CS1 maint: multiple names: authors list (link)
  8. Pomorski, P.; Krzemiński, P.; Wasik, A.; Wierzbicka, K.; Barańska, J.; Kłopocka, W. (July 2007). "Actin dynamics in Amoeba proteus motility". Protoplasma. 231 (1–2): 31–41. doi:10.1007/s00709-007-0243-1. PMID   17602277.
  9. Miyoshi, Hiromi; Kagawa, Yuki; Tsuchiya, Yoshimi (March 2001). "Chaotic behavior in the locomotion ofamoeba proteus". Protoplasma. 216 (1–2): 66–70. doi:10.1007/BF02680132. PMID   11732198.
  10. Hofstatter PG, Brown MW, Lahr DJ (November 2018). "Comparative Genomics Supports Sex and Meiosis in Diverse Amoebozoa". Genome Biol Evol. 10 (11): 3118–3128. doi:10.1093/gbe/evy241. PMC   6263441 . PMID   30380054.