BIM-1

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Contents

BIM-1
Bis(indolyl)maleimide.svg
Names
IUPAC name
3-{1-[3-(Dimethylamino)propyl]-1H-indol-3-yl}-4-(1H-indol-3-yl)-1H-pyrrole-2,5-dione
Other names
RBT205 INHIBITOR, BI1
Identifiers
3D model (JSmol)
ChemSpider
DrugBank
ECHA InfoCard 100.122.321 OOjs UI icon edit-ltr-progressive.svg
EC Number
  • 603-702-0
MeSH bisindolylmaleimide
PubChem CID
UNII
  • Key: QMGUOJYZJKLOLH-UHFFFAOYSA-N
  • InChI=1S/C25H24N4O2/c1-28(2)12-7-13-29-15-19(17-9-4-6-11-21(17)29)23-22(24(30)27-25(23)31)18-14-26-20-10-5-3-8-16(18)20/h3-6,8-11,14-15,26H,7,12-13H2,1-2H3,(H,27,30,31)
  • O=C5C(\c2c1ccccc1[nH]c2)=C(\c4c3ccccc3n(c4)CCCN(C)C)C(=O)N5
Properties
C25H24N4O2
Molar mass 412.493 g·mol−1
AppearanceOrange solid
Density 1.3 g/cm3
Melting point 208 to 210 °C (406 to 410 °F; 481 to 483 K)
Solubility in DMSO Soluble[ vague ]
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).

BIM-1 (GF 109203X) and the related compounds BIM-2, BIM-3, and BIM-8 are bisindolylmaleimide-based protein kinase C (PKC) inhibitors. These inhibitors also inhibit PDK1 explaining the higher inhibitory potential of LY33331 compared to the other BIM compounds a bisindolylmaleimide inhibitor toward PDK1. [1] [2]

Function

BIM-1 is present in the structure of PKCiota [3] (residue 574-turn [4] motif). It needs to be phosphorylated towards a PKCbeta-specific inhibitor site-directed mutagenesis of the compound for its full activation [5] and co-crystallized as an asymmetric pair which is mediated by 3-phosphoinositide-dependent protein kinase-1 (PDK1) [6] are downstream characteristics of PKCs and PKB/AKT. [7]

Scope

The bound BIM-1 inhibitor blocks bilobal [8] interactions, the ATP-binding site, features an ATP-competitive inhibitor, 2-methyl-1H-indol-3-yl-BIM-1, [8] the crystal structure [8] and catalytic subunit with a 20-amino acid substrate analog inhibitor structure is bilobal MgATP a transport protein that provide a more precise description of which is influenced by lobe-lobe interactions binding in cells expressing both forms a pair of kinase-inhibitor complexes [7] with ferritin in a soluble and non-toxic form (Poisson-Boltzmann [9] ) and a portion of the inhibitor peptide [10] a lysine residue, has been shown to be involved in ATP binding.

Interactions

The PKCiota-BIM-1 complex [4] interacts with the zinc finger of lambda/iota PKC characterization of lambda-interacting protein (LIP) [5] (lambda-interacting protein; a selective activator of lambda/iota PKC). Phosphorylation of a PKC induces a conformation leading to import of a PKC into the nucleus. [11] The entire 587-amino acid coding region of a new PKC isoform, PKC iota. [11] Where Thr-412 [5] [12] (activation loop of the kinase domain) at PKCiota/lambda phosphorylates glyceraldehyde-3-phosphate dehydrogenase (GAPDH) [13] that sort cargo to the anterograde pathway [14] the phosphorylation pathway(s) involved in this phenomenon [2] mimic glutamate and can adopt two limiting diastereomeric (syn and anti) conformation [15] biosynthetically related indolocarbazole analogs [16] and in Proto-oncogene serine/threonine-protein kinase Pim-1-Peptide as a phosphorylation target including itself. The bound BIM-1 inhibitor blocks the ATP-binding site and puts the kinase domain into an intermediate open [7] conformation. [4] The value of such calculations lies in understanding [9] a variant was designed which showed improved binding characteristics [17] of configurationally stable atropisomeric bisindolylmaleimides [15] where the two kinase domains, and two different inhibitor conformers bind in different orientations, [7] the hinge region of staurosporine [18] -Pim-1 resembles [19] co-crystallized [8] as an asymmetric pair of biosynthetically 'related' indolocarbazole analogs. It is a modulator of the 5-HT2A receptor. [20]

References

  1. ↑ Komander D, Kular GS, Schüttelkopf AW, Deak M, Prakash KR, Bain J, Elliott M, Garrido-Franco M, Kozikowski AP, Alessi DR, van Aalten DM (February 2004). "Interactions of LY333531 and other bisindolyl maleimide inhibitors with PDK1". Structure. 12 (2): 215–26. doi: 10.1016/j.str.2004.01.005 . PMID   14962382.
  2. 1 2 Cartee L, Smith R, Dai Y, Rahmani M, Rosato R, Almenara J, Dent P, Grant S (June 2002). "Synergistic induction of apoptosis in human myeloid leukemia cells by phorbol 12-myristate 13-acetate and flavopiridol proceeds via activation of both the intrinsic and tumor necrosis factor-mediated extrinsic cell death pathways". Mol. Pharmacol. 61 (6): 1313–21. doi:10.1124/mol.61.6.1313. PMID   12021392.
  3. ↑ Baldwin RM, Parolin DA, Lorimer IA (June 2008). "Regulation of glioblastoma cell invasion by PKC iota and RhoB". Oncogene. 27 (25): 3587–95. doi: 10.1038/sj.onc.1211027 . PMID   18212741.
  4. 1 2 3 Messerschmidt A, Macieira S, Velarde M, Bädeker M, Benda C, Jestel A, Brandstetter H, Neuefeind T, Blaesse M (September 2005). "Crystal structure of the catalytic domain of human atypical protein kinase C-iota reveals interaction mode of phosphorylation site in turn motif". J Mol Biol. 352 (4): 918–31. doi:10.1016/j.jmb.2005.07.060. PMID   16125198.
  5. 1 2 3 Diaz-Meco MT, Municio MM, Sanchez P, Lozano J, Moscat J (January 1996). "Lambda-interacting protein, a novel protein that specifically interacts with the zinc finger domain of the atypical protein kinase C isotype lambda/iota and stimulates its kinase activity in vitro and in vivo". Mol Cell Biol. 16 (1): 105–14. doi:10.1128/mcb.16.1.105. PMC   230983 . PMID   8524286.
  6. ↑ Peifer C, Alessi DR (December 2008). "Small-molecule inhibitors of PDK1". ChemMedChem. 3 (12): 1810–38. doi:10.1002/cmdc.200800195. PMID   18972468. S2CID   29838120.
  7. 1 2 3 4 Gassel M, Breitenlechner CB, König N, Huber R, Engh RA, Bossemeyer D (May 2004). "The protein kinase C inhibitor bisindolyl maleimide 2 binds with reversed orientations to different conformations of protein kinase A". J Biol Chem. 279 (22): 23679–90. doi: 10.1074/jbc.M314082200 . PMID   14996846.
  8. 1 2 3 4 Grodsky Grodsky N, Li Y, Bouzida D, Love R, Jensen J, Nodes B, Nonomiya J, Grant S (November 2006). "Structure of the catalytic domain of human protein kinase C beta II complexed with a bisindolylmaleimide inhibitor". Biochemistry. 45 (47): 13970–81. doi:10.1021/bi061128h. PMID   17115692.
  9. 1 2 Page CS, Bates PA (December 2006). "Can MM-PBSA calculations predict the specificities of protein kinase inhibitors?". J. Comput. Chem. 27 (16): 1990–2007. Bibcode:2006JCoCh..27.1990P. doi:10.1002/jcc.20534. PMID   17036304. S2CID   23077429.
  10. ↑ Knighton DR, Zheng JH, Ten Eyck LF, Ashford VA, Xuong NH, Taylor SS, Sowadski JM (July 1991). "Crystal structure of the catalytic subunit of cyclic adenosine monophosphate-dependent protein kinase". Science. 253 (5018): 407–14. Bibcode:1991Sci...253..407K. doi:10.1126/science.1862342. PMID   1862342.
  11. 1 2 White WO, Seibenhener ML, Wooten MW (January 2002). "Phosphorylation of tyrosine 256 facilitates nuclear import of atypical protein kinase C". J Cell Biochem. 85 (1): 42–53. doi:10.1002/jcb.10101. PMID   11891849. S2CID   221311830.
  12. ↑ Cantin GT, Yi W, Lu B, Park SK, Xu T, Lee JD, Yates JR (March 2008). "Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis". J Proteome Res. 7 (3): 1346–51. doi:10.1021/pr0705441. PMID   18220336.
  13. ↑ Tisdale EJ. (December 2003). "Rab2 interacts directly with atypical protein kinase C (aPKC) iota/lambda and inhibits aPKCiota/lambda-dependent glyceraldehyde-3-phosphate dehydrogenase phosphorylation". J Biol Chem. 278 (52): 52424–30. doi: 10.1074/jbc.M309343200 . PMID   14570876.
  14. ↑ Tisdale EJ, Artalejo CR (March 2006). "Src-dependent aprotein kinase C iota/lambda (aPKCiota/lambda) tyrosine phosphorylation is required for aPKCiota/lambda association with Rab2 and glyceraldehyde-3-phosphate dehydrogenase on pre-golgi intermediates". J Biol Chem. 281 (13): 8436–42. doi: 10.1074/jbc.M513031200 . PMC   3742308 . PMID   16452474.
  15. 1 2 Barrett S, Bartlett S, Bolt A, Ironmonger A, Joce C, Nelson A, Woodhall T (October 2005). "Configurational stability of bisindolylmaleimide cyclophanes: from conformers to the first configurationally stable, atropisomeric bisindolylmaleimides". Chemistry: A European Journal. 11 (21): 6277–85. Bibcode:2005ChEuJ..11.6277B. doi:10.1002/chem.200500520. PMID   16075446.
  16. ↑ Sánchez C, Méndez C, Salas JA (December 2006). "Indolocarbazole natural products: occurrence, biosynthesis, and biological activity". Nat Prod Rep. 23 (6): 1007–45. doi:10.1039/B601930G. hdl: 10651/42078 . PMID   17119643.
  17. ↑ Bandeiras TM, Hillig RC, Matias PM, Eberspaecher U, Fanghänel J, Thomaz M, Miranda S, Crusius K, Pütter V, Amstutz P, Gulotti-Georgieva M, Binz HK, Holz C, Schmitz AA, Lang C, Donner P, Egner U, Carrondo MA, Müller-Tiemann B (April 2008). "Structure of wild-type Plk-1 kinase domain in complex with a selective DARPin". Acta Crystallogr D. 64 (4): 339–53. doi:10.1107/S0907444907068217. PMID   18391401.
  18. ↑ Lee SK, Stern PH (October 2000). "Divergent effects of protein kinase C (PKC) inhibitors staurosporine and bisindolylmaleimide I (GF109203X) on bone resorption". Biochem. Pharmacol. 60 (7): 923–6. doi:10.1016/S0006-2952(00)00418-4. PMID   10974200.
  19. ↑ M.D.Jacobs; J.Black; O.Futer; L.Swenson; B.Hare; M.Fleming; K.Saxena (May 2005). "Pim-1 ligand-bound structures reveal the mechanism of serine/threonine kinase inhibition by LY294002". J Biol Chem. 280 (14): 13728–13734. doi: 10.1074/jbc.M413155200 . PMID   15657054.
  20. ↑ Minami K, Minami M, Harris RA (June 1997). "Inhibition of 5-hydroxytryptamine type 2A receptor-induced currents by n-alcohols and anesthetics". J Pharmacol Exp Ther. 281 (3): 1136–43. PMID   9190846.{{cite journal}}: CS1 maint: multiple names: authors list (link)
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