Bacillus fastidiosus

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Bacillus fastidiosus
Scientific classification OOjs UI icon edit-ltr.svg
Domain: Bacteria
Phylum: Bacillota
Class: Bacilli
Order: Bacillales
Family: Bacillaceae
Genus: Bacillus
Species:
B. fastidiosus
Binomial name
Bacillus fastidiosus
den Dooren de Jong 1929 (Approved Lists 1980)

Bacillus fastidiosus is an aerobic, motile, rod-shaped bacterium that has been isolated from soil and poultry litter. [1] The species was first isolated and described by the scientist Den Dooren de Jong in 1929. [2] This organism is a mesophile that contains ellipsoidal spores that do not cause swelling of the sporangia. Bacillus fastidiosus is only able to grow in the presence of uric acid, allantoin, or allantoic acid. [1]

This species has been recently transferred into the genus Metabacillus. [3] The correct nomenclature is Metabacillus fastidiosus.

Morphology

Bacillus fastidiosus has peritrichous flagella meaning that it has flagella in a uniform distribution all over the cell that it uses for motility. [2] Cells of this species are about 5 μm long and 1.5 μm wide. [4] They may contain endospores that can be located centrally, paracentrally, or subterminally. This bacterium is typically grown on 1% uric acid agar and colonies can have a rhizoid appearance. Colonies are typically opaque and may become yellowish over time. The cells will produce ammonia as a byproduct of their metabolism, which means that their microenvironment will become highly alkaline. This creates a self-limiting cycle that creates zones of inhibition around each colony. B. fastidiosus is catalase and oxidase positive. It is unable to produce acid or gas when grown in the presence of carbohydrates such as glucose. B. fastidiosus is able to hydrolyze urea, but it is unable to hydrolyze casein, gelatin, or starch. [1]

Metabolism

Bacillus fastidious has the ability to use uricase to degrade uric acid to allantoin, and then use allantoinase to degrade allantoin to allantoate. [5] It also has the ability to further break down allantoate to ammonia and ureidoglycolate via the enzyme allantoate amidohydrolase. [5] The liberation of ammonia causes its local environment to rise to a pH between 8 and 9. However, attempts to grow Bacillus fastidious at those higher pH's without urea present were unsuccessful. [4] It is able to metabolize ureidoglycolate further into urea and glyoxylate using the enzyme ureidoglycolase. [5] Bacillus fastidious is also able to use urease to degrade urea. [5]

Related Research Articles

<i>Proteus</i> (bacterium) Genus of bacteria

Proteus is a genus of Gram-negative bacteria. It is a rod shaped, aerobic and motile bacteria, which is able to migrate across surfaces due its “swarming” characteristic in temperatures between 20 and 37 °C. Their size generally ranges from 0.4 to 0.8 μm in diameter and 1.0–3.0 μm in length. They tend to have an ammonia smell. Proteus bacilli are widely distributed in nature as saprophytes, being found in decomposing animal matter, sewage, manure soil, the mammalian intestine, and human and animal feces. They are opportunistic pathogens, commonly responsible for urinary and septic infections, often nosocomial.

<span class="mw-page-title-main">Allantoin</span> Chemical compound

Allantoin is a chemical compound with formula C4H6N4O3. It is also called 5-ureidohydantoin or glyoxyldiureide. It is a diureide of glyoxylic acid. Allantoin is a major metabolic intermediate in most organisms including animals, plants and bacteria. It is produced from uric acid, which itself is a degradation product of nucleic acids, by action of urate oxidase (uricase). Allantoin also occurs as a natural mineral compound (IMA symbol Aan).

<i>Proteus mirabilis</i> Species of bacterium

Proteus mirabilis is a Gram-negative, facultatively anaerobic, rod-shaped bacterium. It shows swarming motility and urease activity. P. mirabilis causes 90% of all Proteus infections in humans. It is widely distributed in soil and water. Proteus mirabilis can migrate across the surface of solid media or devices using a type of cooperative group motility called swarming. Proteus mirabilis is most frequently associated with infections of the urinary tract, especially in complicated or catheter-associated urinary tract infections.

Nitrosomonas europaea is a Gram-negative obligate chemolithoautotroph that can derive all its energy and reductant for growth from the oxidation of ammonia to nitrite and lives in several places such as soil, sewage, freshwater, the walls of buildings and on the surface of monuments especially in polluted areas where the air contains high levels of nitrogen compounds.

<i>Nitrosomonas</i> Genus of bacteria

Nitrosomonas is a genus of Gram-negative bacteria, belonging to the Betaproteobacteria. It is one of the five genera of ammonia-oxidizing bacteria and, as an obligate chemolithoautotroph, uses ammonia as an energy source and carbon dioxide as a carbon source in presence of oxygen. Nitrosomonas are important in the global biogeochemical nitrogen cycle, since they increase the bioavailability of nitrogen to plants and in the denitrification, which is important for the release of nitrous oxide, a powerful greenhouse gas. This microbe is photophobic, and usually generate a biofilm matrix, or form clumps with other microbes, to avoid light. Nitrosomonas can be divided into six lineages: the first one includes the species Nitrosomonas europea, Nitrosomonas eutropha, Nitrosomonas halophila, and Nitrosomonas mobilis. The second lineage presents the species Nitrosomonas communis, N. sp. I and N. sp. II, meanwhile the third lineage includes only Nitrosomonas nitrosa. The fourth lineage includes the species Nitrosomonas ureae and Nitrosomonas oligotropha and the fifth and sixth lineages include the species Nitrosomonas marina, N. sp. III, Nitrosomonas estuarii and Nitrosomonas cryotolerans.

Members of the genus Selenomonas are referred to trivially as selenomonads. The genus Selenomonas constitutes a group of motile crescent-shaped bacteria and includes species living in the gastrointestinal tracts of animals, in particular the ruminants. A number of smaller forms discovered with the light microscope are now in culture but many, especially the large selenomonads are not, owing to their fastidious and incompletely known growth requirements.

<i>Alcaligenes</i> Genus of bacteria

Alcaligenes is a genus of Gram-negative, aerobic, rod-shaped bacteria in the order of Burkholderiales.

<i>Bacillus megaterium</i> Species of bacterium

Bacillus megaterium is a rod-like, Gram-positive, mainly aerobic, spore forming bacterium found in widely diverse habitats. It has a cell length up to 100 μm and a diameter of 0.1 μm, which is quite large for bacteria. The cells often occur in pairs and chains, where the cells are joined by polysaccharides on the cell walls.

Marinobacter hydrocarbonoclasticus is a species of bacteria found in sea water which are able to degrade hydrocarbons. The cells are rod-shaped and motile by means of a single polar flagellum.

<span class="mw-page-title-main">Allantoicase</span>

Allantoicase is an enzyme (EC 3.5.3.4) that in humans is encoded by the ALLC gene. Allantoicase catalyzes the chemical reaction

Sporosarcina pasteurii formerly known as Bacillus pasteurii from older taxonomies, is a gram positive bacterium with the ability to precipitate calcite and solidify sand given a calcium source and urea; through the process of microbiologically induced calcite precipitation (MICP) or biological cementation. S. pasteurii has been proposed to be used as an ecologically sound biological construction material. Researchers studied the bacteria in conjunction with plastic and hard mineral; forming a material stronger than bone. It is a commonly used for MICP since it is non-pathogenic and is able to produce high amounts of the enzyme urease which hydrolyzes urea to carbonate and ammonia.

Geobacillus thermoglucosidasius is a thermophilic gram-positive bacterium, and a member of the Bacillota phylum. It was first isolated from soil in Japan in 1983.

Amphibacillus xylanus is a gram-positive-spore forming bacterium with cells 0.3 μm to 0.5 μm in diameter and 0.9 μm to 1.9 μm in length. A. xylanus is a facultative anaerobic organism which can grow in several different environments. Its success in a multitude of environments stems from multiple metabolic pathways, each with high ATP yields. It is flagellated and motile. It grows best at pH 8.0-10.0 but not at pH 7.0. It is catalase and oxidase negative. This helps to explain better the unique method of metabolism on which the organism relies.

Alcanivorax pacificus is a pyrene-degrading marine gammaproteobacterium. It is of the genus Alcanivorax, a group of marine bacteria known for degrading hydrocarbons. When originally proposed, the genus Alcanivorax comprised six distinguishable species. However, A. pacificus, a seventh strain, was isolated from deep sea sediments in the West Pacific Ocean by Shanghai Majorbio Bio-pharm Technology Co., Ltd. in 2011. A. pacificus’s ability to degrade hydrocarbons can be employed for cleaning up oil-contaminated oceans through bioremediation. The genomic differences present in this strain of Alcanivorax that distinguish it from the original consortium are important to understand to better utilize this bacteria for bioremediation.

Acidiphilium cryptum is a species of heterotrophic bacteria, the type species of its genus. It is gram-negative, aerobic, mesophilic and rod-shaped. It does not form endospores and some cells are motile by means of one polar flagellum or two lateral flagella Lhet2 is the type strain.

Lysinibacillus fusiformis is a gram-positive, rod-shaped bacterium of the genus Lysinibacillus. Scientists have yet to completely characterize this microbe's pathogenic nature. Though little is known about this organism, several genome sequencing projects for various strains of L. fusiformis are currently underway.

Salibacterium is a genus of Gram-positive bacteria from the family of Bacillaceae. The type species is Salibacterium halotolerans.

Alkalihalobacillus is a genus of gram-positive or gram-variable rod-shaped bacteria in the family Bacillaceae from the order Bacillales. The type species of this genus is Alkalihalobacillus alcalophilus.

Metabacillus is a genus of rod-shaped bacteria exhibiting Gram-positive or Gram-variable staining in the family Bacillaceae within the order Bacillales. The type species for this genus is Metabacillus fastidiosus.

Niallia is a genus of Gram-Positive rod-shaped bacteria in the family Bacillaceae from the order Bacillales. The type species of this genus is Niallia circulans.

References

  1. 1 2 3 Garrity, George; Vos, Paul; Jones, Dorothy; Krieg, Noel; Schleifer, Karl-Heinz; Ludwig, Wolfgang; Rainey, Fred; Whitman, William (2009). Bergey's Manual of Systematic Bacteriology (2nd ed.). New York: Springer. p. 99. ISBN   978-0387950419.
  2. 1 2 Kaltwasser, H. (22 March 1971). "Studies on the Physiology of Bacillus fastidious". Journal of Bacteriology. 107 (3): 780–786. doi:10.1128/jb.107.3.780-786.1971. PMC   247000 . PMID   5095289.
  3. Patel, Sudip; Gupta, Radhey S. (2020-01-01). "A phylogenomic and comparative genomic framework for resolving the polyphyly of the genus Bacillus: Proposal for six new genera of Bacillus species, Peribacillus gen. nov., Cytobacillus gen. nov., Mesobacillus gen. nov., Neobacillus gen. nov., Metabacillus gen. nov. and Alkalihalobacillus gen. nov". International Journal of Systematic and Evolutionary Microbiology. 70 (1): 406–438. doi: 10.1099/ijsem.0.003775 . ISSN   1466-5026. PMID   31617837.
  4. 1 2 LEADBETTER, E. R.; HOLT, S. C. (12 January 1968). "The Fine Structure of Bacillus fastidiosus". Journal of General Microbiology. 52 (2): 299–306. doi: 10.1099/00221287-52-2-299 .
  5. 1 2 3 4 Bongaerts, G. P. A; Vogels, G. D (1976). "Uric Acid Degradation by Bacillus fastidiosus Strains". Journal of Bacteriology. 125 (2): 689–697. doi:10.1128/jb.125.2.689-697.1976. PMC   236130 . PMID   1245468.