U.S. Route 27 or U.S. Highway 27 (US 27) is a north–south United States Numbered Highway in the Southern and Midwestern U.S. The southern terminus is at US 1 in Miami, Florida. The northern terminus is at Interstate 69 (I-69) in Fort Wayne, Indiana. From Miami, it goes up the center of Florida, then west to Tallahassee, Florida, and north through such cities and towns as Columbus, Georgia; Rome, Georgia; Chattanooga, Tennessee; Lexington, Kentucky; Cincinnati, Ohio; Oxford, Ohio; Richmond, Indiana; and Fort Wayne, Indiana. It once extended north through Lansing, Michigan, to Cheboygan, Mackinaw City, and, for about three years, even as far as St. Ignace. US 27 was first signed in 1926, replacing what had been the eastern route of the Dixie Highway in many states.
U.S. Route 319 is a spur of US 19. It runs for 303 miles (488 km) from US 98 at the foot of the John Gorrie Memorial Bridge across from downtown Apalachicola, Florida to US 1/SR 4 in Wadley, Georgia, through the Panhandle of Florida and the southern portion of Georgia.
State Road 944, locally known as Hialeah Drive and North 54th Street, is a 5.822-mile-long (9.370 km) east–west street spanning Hialeah and Miami, Florida. The western terminus is an intersection with Okeechobee Road in Hialeah; its eastern terminus is an intersection with Biscayne Boulevard. Along with the north–south Palm Avenue, Hialeah Drive is a baseline for addresses in the City of Hialeah.
State Road 363 is a north–south route in the Big Bend region of Florida.
State Road 267 is a road in the U.S. state of Florida. It has a north–south state route in the eastern Florida panhandle, west of Tallahassee.
State Road 265 is a short north–south route in Tallahassee.
State Road 371 (SR 371) runs through Tallahassee as a primarily east west route. In 2018, the Florida Legislature designated the Orange Avenue section of the roadway as C.K. Steele Memorial Highway.
State Road 366 is a short east–west route in Tallahassee between U.S. Route 27 (US 27) and SR 20. It is primarily known as Pensacola Street throughout its path, due to its generally westward course toward the Florida city of Pensacola, although an eastbound section is known as St. Augustine Road, heading toward St. Augustine.
State Road 20 is a 358.154-mile-long (576.393 km) east-to-west route across northern Florida and the Florida Panhandle in the United States.
State Road 53 (SR 53) is a 19.170-mile-long (30.851 km) north–south state highway in the panhandle of northern Florida. Its southern terminus is an interchange with Interstate 10 near Lee and Madison; the northern terminus is at the Georgia state line near Cherry Lake, Florida. Part of SR 53, from Harvey Greene Drive north to SR 14, is also signed SR 14 Truck, a rare special state highway in Florida.
State Road 12 (SR 12) is an east–west route in the Florida Panhandle, running from SR 20 in Bristol to U.S. Route 27 (US 27) in Havana.
State Road 263 is a north–south route that forms the western portion of Capital Circle, Tallahassee's "beltway".
State Road 25 is a state highway in the U.S. state of Florida. It is mainly signed as U.S. Highways:
State Road 789 is a 17.5-mile-long road along the Florida’s Gulf Coast that spans Bird Key, St. Armands Key, and Lido Key, in Sarasota; Longboat Key ; and Anna Maria Island. The southern terminus is the intersection of the John Ringling Causeway and Tamiami Trail in Sarasota; the northern terminus is the intersection of Gulf Drive, North and Manatee Avenue., West in Holmes Beach. Much of the northernmost five miles (8 km) has been designated Bradenton Beach Scenic Highway.
U.S. Highway 27 (US 27) in Florida is a north–south United States Numbered Highway. It runs 496.352 miles (798.801 km) from the Miami metropolitan area northwest to the Tallahassee metropolitan area. Throughout the state, US 27 has been designated the Claude Pepper Memorial Highway by the Florida Legislature. It was named after long-time Florida statesperson Claude Pepper, who served in both the U.S. Senate and House of Representatives. The stretch running from Miami to South Bay was originally designated the Thomas E. Will Memorial Highway by the Florida Legislature in 1937 when that portion was known as State Road 26 (SR 26). Thomas E. Will, the founder of Okeelanta, had worked for almost 20 years to get the state to build a road from Miami to the area south of Lake Okeechobee. For most of its length in the state, US 27 is a divided highway.
U.S. Route 221 (US 221) in Florida is a north–south United States Highway. It runs 40 miles (64 km) from Perry northwest to the Georgia State Line in Taylor, Madison and Jefferson Counties. The entire route is inventoried by FDOT as the northern section of unsigned State Road 55. A short overlap with US 90 exists in Greenville.
State Road 684 is a state road located in Manatee County, Florida, United States. The road runs from an intersection with SR 789 in Bradenton Beach east to U.S. Route 41 and unsigned SR 55 in South Bradenton. SR 684 is known as Cortez Road its entire length and varies from a two-lane road between Bradenton Beach and Cortez to a four- to six-lane road east of Cortez. The easternmost portion of SR 684 is concurrent with US 41 and is unsigned.
State Road 368 (SR 368) is a short state road an east–west "bypass route", locally known as 23rd Street, in and around the vicinity of Panama City, Florida. The road is entirely four-lanes wide with center left-turn lanes where available, and is divided only at its eastern terminus. No roads overlap SR 368 or vice versa.
State Road 160 (FL 160) is a 0.114-mile-long (0.183 km) state highway in Tallahassee, Leon County, Florida, that connects Florida State Road 61 and County Road 0352 and U.S. Route 319 north of Interstate 10 in northern Tallahassee.
State Road 162 (FL 162) is an unsigned 0.292-mile-long (0.470 km) state highway in Tallahassee, Leon County, Florida, that connects Florida State Road 61 and U.S. Route 319 south of Interstate 10 in northern Tallahassee.
US 98 near Ochlockonee Bay 
US 319 in Crawfordville 
US 27 / US 90 in Tallahassee 
I-10 in Tallahassee 
US 319 / SR 35 towards Thomasville, GA 
