Sir John Chamberlain (c. 1560 - 1617) of Prestbury, Gloucestershire was an English landowner and politician. [1]
He was the elder son, and the armigerous heir, of the thrice-married diplomat Sir Thomas Chamberlain of Prestbury, Gloucestershire (died 1580) [2] by his second wife Joan Luddington (died 1565), widow of alderman John Machell, citizen and Clothworker of London (who died 1558). [3] [4] He was the elder brother of Edmund Chamberlain of Maugersbury, by Stow-on-the-Wold (died 1634), and of a sister named Theodosia; and he had both an elder and a younger half-brother, both named Thomas Chamberlain. Thomas the younger was of Oddington, Gloucestershire. [2] John married Elizabeth, daughter of Sir John Thynne, but had no children. [1]
He matriculated from Trinity College, Oxford in 1579. [5] He served as High Sheriff of Gloucestershire in 1596-7. [1] He has been identified as the man who sat for Clitheroe in 1593 and St Germans in 1597. [1] With many others, he was knighted on 23 July 1603 in the King's garden at Whitehall before the coronation. [6] His will was proved in 1617. [7]