This is a listing of Australian rules footballers who made their senior debut for an Australian Football League (AFL) club in 2000.
| Name | Club | Age at debut | Round debuted | Games (As of end of 2014) | Goals (As of end of 2014) | Notes | 
|---|---|---|---|---|---|---|
| Michael Doughty | Adelaide | 20 years, 255 days | 6 [1] | 231 | 46 | |
| Robert Shirley | Adelaide | 19 years, 290 days | 3 [2] | 151 | 28 | |
| Rhett Biglands | Adelaide | 22 years, 217 days | 5 [3] | 134 | 59 | |
| Scott Welsh | Adelaide | 21 years, 332 days | 1 [4] | 129 | 270 | Previously played for North Melbourne. | 
| Matthew Clarke | Adelaide | 26 years, 175 days | 1 [5] | 118 | 19 | Previously played for Brisbane. | 
| Andrew Crowell | Adelaide | 22 years, 209 days | 1 [6] | 44 | 15 | |
| James Byrne | Adelaide | 21 years, 323 days | 1 [7] | 24 | 4 | |
| Ricky O'Loughlin | Adelaide | 20 years, 279 days | 5 [8] | 9 | 2 | Brother of Michael O'Loughlin. | 
| Justin Cicolella | Adelaide | 21 years, 195 days | 3 [9] | 5 | 1 | |
| Jonathan Brown | Brisbane Lions | 18 years, 162 days | 5 [10] | 256 | 594 | Son of Brian Brown. | 
| Aaron Shattock | Brisbane Lions | 19 years, 342 days | 5 [11] | 57 | 15 | |
| Craig Bolton | Brisbane Lions | 19 years, 287 days | 1 [12] | 29 | 10 | |
| Damian Cupido | Brisbane Lions | 18 years, 71 days | 11 [13] | 13 | 16 | |
| Trent Knobel | Brisbane Lions | 20 years, 17 days | 14 [14] | 13 | 10 | |
| Michael Martin | Brisbane Lions | 23 years, 23 days | 2 [15] | 10 | 0 | Previously played for Western Bulldogs. | 
| Nathan Clarke | Brisbane Lions | 20 years, 256 days | 18 [16] | 6 | 4 | |
| Stefan Carey | Brisbane Lions | 24 years, 48 days | 1 [17] | 3 | 1 | Previously played for Sydney. | 
| Shannon Rusca | Brisbane Lions | 19 years, 218 days | 3 [18] | 2 | 0 | |
| Ryan Houlihan | Carlton | 18 years, 120 days | 11 [19] | 201 | 127 | |
| Trent Hotton | Carlton | 26 years, 103 days | 1 [20] | 61 | 36 | Previously played for Collingwood | 
| Michael Mansfield | Carlton | 28 years, 218 days | 1 [21] | 54 | 18 | Previously played for Geelong. | 
| Scott Freeborn | Carlton | 21 years, 324 days | 5 [22] | 48 | 17 | |
| Andrew Merrington | Carlton | 21 years, 203 days | 15 [23] | 18 | 11 | |
| Stephen O'Reilly | Carlton | 27 years, 138 days | 3 [24] | 12 | 1 | Previously played for Geelong and Fremantle. | 
| Adam Chatfield | Carlton | 21 years, 23 days | 19 [25] | 1 | 0 | |
| Ben Johnson | Collingwood | 18 years, 343 days | 1 [26] | 235 | 70 | |
| Shane O'Bree | Collingwood | 20 years, 364 days | 1 [27] | 227 | 84 | Previously played for Brisbane. | 
| Leon Davis | Collingwood | 18 years, 270 days | 1 [28] | 225 | 270 | |
| Josh Fraser | Collingwood | 18 years, 68 days | 1 [29] | 200 | 163 | |
| Rhyce Shaw | Collingwood | 18 years, 251 days | 16 [30] | 94 | 20 | |
| Steven McKee | Collingwood | 21 years, 292 days | 5 [31] | 64 | 13 | Previously played for Richmond. | 
| Andrew Dimattina | Collingwood | 22 years, 207 days | 13 [32] | 28 | 6 | Brother of Paul and son of Frank Dimattina. | 
| Damien Adkins | Collingwood | 19 years, 64 days | 1 [33] | 22 | 8 | |
| Andrew Ukovic | Collingwood | 21 years, 111 days | 1 [34] | 17 | 9 | Previously played for Essendon. | 
| Dale Baynes | Collingwood | 19 years, 180 days | 12 [35] | 1 | 0 | |
| Brad Smith | Collingwood | 22 years, 325 days | 12 [36] | 1 | 0 | |
| Aaron Henneman | Essendon | 19 years, 159 days | 11 [37] | 58 | 5 | |
| Jonathon Robran | Essendon | 27 years, 147 days | 2 [38] | 8 | 3 | Son of Barrie Robran and Brother of Matthew Robran. Previously played for Hawthorn. | 
| Matthew Pavlich | Fremantle | 18 years, 99 days | 5 [39] | 313 | 629 | |
| Paul Hasleby | Fremantle | 18 years, 273 days | 1 [40] | 208 | 131 | |
| Troy Cook | Fremantle | 23 years, 212 days | 15 [41] | 150 | 66 | Previously played for Sydney. | 
| Leigh Brown | Fremantle | 18 years, 17 days | 1 [42] | 63 | 20 | |
| Troy Longmuir | Fremantle | 20 years, 291 days | 1 [43] | 55 | 36 | Previously played for Melbourne. | 
| Brendon Fewster | Fremantle | 26 years, 67 days | 1 [44] | 37 | 16 | Previously played for West Coast. | 
| Ben Cunningham | Fremantle | 18 years, 257 days | 22 [45] | 33 | 19 | |
| Brad Bootsma | Fremantle | 27 years, 123 days | 8 [46] | 23 | 7 | Father of Josh Bootsma. | 
| Daniel Schell | Fremantle | 20 years, 150 days | 15 [47] | 16 | 11 | |
| Joel Corey | Geelong | 18 years, 134 days | 17 [48] | 276 | 79 | |
| Cameron Ling | Geelong | 19 years, 76 days | 10 [49] | 246 | 139 | |
| Paul Chapman | Geelong | 18 years, 204 days | 12 [50] | 251 | 336 | |
| Cameron Mooney | Geelong | 20 years, 278 days | 17 [51] | 210 | 295 | Previously played for North Melbourne. | 
| David Spriggs | Geelong | 19 years, 46 days | 1 [52] | 64 | 20 | |
| Daniel Foster | Geelong | 18 years, 76 days | 9 [53] | 17 | 0 | |
| Danny O'Brien | Geelong | 20 years, 48 days | 3 [54] | 8 | 6 | |
| Marcus Baldwin | Geelong | 20 years, 280 days | 21 [55] | 5 | 5 | |
| Chance Bateman | Hawthorn | 18 years, 278 days | 3 [56] | 177 | 67 | |
| Lance Picioane | Hawthorn | 19 years, 363 days | 13 [57] | 58 | 24 | Previously played for Adelaide. | 
| Brett Johnson | Hawthorn | 18 years, 239 days | 16 [58] | 38 | 10 | |
| Barry Young | Hawthorn | 29 years, 261 days | 1 [59] | 13 | 0 | Previously played for Richmond and Essendon. | 
| Luke McPharlin | Hawthorn | 18 years, 130 days | 5 [60] | 12 | 3 | |
| Steven Rode | Hawthorn | 20 years, 3 days | 5 [61] | 1 | 0 | |
| Brad Green | Melbourne | 19 years, 4 days | 2 [62] | 254 | 350 | |
| Cameron Bruce | Melbourne | 20 years, 160 days | 1 [63] | 224 | 210 | |
| Matthew Whelan | Melbourne | 20 years, 141 days | 4 [64] | 150 | 15 | |
| Paul Wheatley | Melbourne | 18 years, 340 days | 2 [65] | 135 | 34 | |
| Simon Godfrey | Melbourne | 19 years, 279 days | 20 [66] | 105 | 23 | |
| Stephen Powell | Melbourne | 23 years, 183 days | 1 [67] | 44 | 43 | Previously played for Western Bulldogs. | 
| Steven Pitt | Melbourne | 26 years, 283 days | 8 [68] | 5 | 2 | Previously played for Collingwood. | 
| James Cook | Melbourne | 26 years, 61 days | 1 [69] | 3 | 8 | Previously played for Carlton and Western Bulldogs. | 
| Troy Makepeace | Kangaroos | 21 years, 25 days | 4 [70] | 139 | 37 | |
| Leigh Colbert | Kangaroos | 24 years, 298 days | 4 [71] | 104 | 14 | Previously played for Geelong. | 
| Matthew Burton | Kangaroos | 29 years, 296 days | 1 [72] | 77 | 33 | Previously played for Fremantle. | 
| David Calthorpe | Kangaroos | 26 years, 206 days | 1 [73] | 13 | 10 | Previously played for Essendon and Brisbane. | 
| John Spaull | Kangaroos | 22 years, 289 days | 13 [74] | 8 | 5 | |
| Ryan Pagan | Kangaroos | 22 years, 242 days | 1 [75] | 3 | 0 | Son of Denis Pagan. | 
| Brad Stephens | Kangaroos | 21 years, 11 days | 20 [76] | 2 | 0 | |
| Matthew Bishop | Port Adelaide | 24 years, 266 days | 4 [77] | 132 | 4 | Previously played for Melbourne. | 
| Brett Montgomery | Port Adelaide | 26 years, 282 days | 1 [78] | 126 | 51 | Previously played for Western Bulldogs. | 
| Josh Carr | Port Adelaide | 20 years, 1 days | 8 [79] | 124 | 65 | Brother of Matthew Carr. | 
| Brent Guerra | Port Adelaide | 17 years, 365 days | 12 [80] | 65 | 39 | |
| Paul Koulouriotis | Port Adelaide | 18 years, 139 days | 18 [81] | 3 | 0 | |
| Steven Brosnan | Port Adelaide | 21 years, 169 days | 3 [82] | 1 | 0 | |
| Leon Cameron | Richmond | 27 years, 188 days | 1 [83] | 84 | 40 | Previously played for Western Bulldogs. | 
| Aaron Fiora | Richmond | 19 years, 5 days | 7 [84] | 78 | 25 | |
| Ty Zantuck | Richmond | 18 years, 159 days | 20 [85] | 68 | 20 | Son of Shane Zantuck. | 
| Clinton King | Richmond | 21 years, 350 days | 1 [86] | 58 | 10 | Previously played for Sydney and Collingwood. | 
| Royce Vardy | Richmond | 20 years, 3 days | 9 [87] | 34 | 3 | |
| Andrew Mills | Richmond | 18 years, 289 days | 4 [88] | 14 | 5 | |
| Ezra Poyas | Richmond | 19 years, 0 days | 3 [89] | 9 | 6 | |
| Ben Haynes | Richmond | 19 years, 19 days | 19 [90] | 5 | 0 | |
| James White | Richmond | 20 years, 127 days | 12 [91] | 4 | 1 | |
| Jason Blake | St Kilda | 19 years, 51 days | 9 [92] | 219 | 38 | |
| Troy Schwarze | St Kilda | 18 years, 101 days | 4 [93] | 71 | 20 | Brother of Ben Schwarze. | 
| Brett Moyle | St Kilda | 20 years, 15 days | 6 [94] | 48 | 11 | |
| Caydn Beetham | St Kilda | 18 years, 63 days | 10 [95] | 37 | 9 | |
| Tony Delaney | St Kilda | 24 years, 76 days | 1 [96] | 33 | 6 | Previously played for Essendon and Fremantle. | 
| Chad Davis | St Kilda | 19 years, 291 days | 2 [97] | 31 | 17 | |
| Justin Plapp | St Kilda | 22 years, 264 days | 1 [98] | 26 | 8 | Previously played for Richmond. | 
| Damian Monkhorst | St Kilda | 30 years, 204 days | 1 [99] | 10 | 0 | Previously played for Collingwood. | 
| Sean Charles | St Kilda | 24 years, 299 days | 1 [100] | 8 | 6 | Previously played for Melbourne and Carlton. | 
| Fred Campbell | St Kilda | 20 years, 11 days | 4 [101] | 7 | 4 | Previously played for Sydney. | 
| Ryan O'Keefe | Sydney | 19 years, 111 days | 10 [102] | 286 | 261 | |
| Jason Ball | Sydney | 27 years, 112 days | 1 [103] | 90 | 45 | Previously played for West Coast. | 
| Andrew Schauble | Sydney | 23 years, 116 days | 1 [104] | 88 | 23 | Previously played for Collingwood. | 
| Stephen Doyle | Sydney | 19 years, 9 days | 20 [105] | 47 | 19 | |
| Ben Fixter | Sydney | 19 years, 15 days | 18 [106] | 27 | 8 | |
| Ryan Fitzgerald | Sydney | 23 years, 148 days | 1 [107] | 10 | 15 | |
| Brett Allison | Sydney | 31 years, 291 days | 1 [108] | 9 | 9 | Previously played for North Melbourne. | 
| Darren Glass | West Coast | 18 years, 323 days | 4 [109] | 270 | 8 | |
| Adam Hunter | West Coast | 19 years, 49 days | 22 [110] | 151 | 86 | |
| Callum Chambers | West Coast | 20 years, 175 days | 10 [111] | 66 | 41 | |
| Kasey Green | West Coast | 20 years, 296 days | 17 [112] | 54 | 8 | |
| Kane Munro | West Coast | 18 years, 141 days | 21 [113] | 18 | 11 | |
| David Haynes | West Coast | 18 years, 359 days | 13 [114] | 46 | 43 | |
| David Antonowicz | West Coast | 20 years, 2 days | 3 [115] | 3 | 0 | |
| Michael O'Brien | West Coast | 19 years, 328 days | 15 [116] | 2 | 0 | |
| Scott Bennett | West Coast | 20 years, 106 days | 7 [117] | 1 | 0 | |
| Robert Murphy | Western Bulldogs | 18 years, 36 days | 19 [118] | 271 | 171 | |
| Nathan Eagleton | Western Bulldogs | 21 years, 122 days | 1 [119] | 197 | 32 | Previously played for Port Adelaide. | 
| Mitch Hahn | Western Bulldogs | 19 years, 4 days | 10 [120] | 181 | 64 | |
| Trent Bartlett | Western Bulldogs | 23 years, 106 days | 1 [121] | 42 | 34 | Previously played for Brisbane. | 
| Luke Penny | Western Bulldogs | 19 years, 117 days | 13 [122] | 35 | 5 | |
| Patrick Wiggins | Western Bulldogs | 19 years, 130 days | 15 [123] | 12 | 5 | |
| Andrew Wills | Western Bulldogs | 28 years, 76 days | 2 [124] | 10 | 6 | Previously played for Geelong and Fremantle. |