| Name | Neighborhood | Type | Summary |
|---|
| American Jewish Museum | Squirrel Hill | Art | Contemporary Jewish art |
| Andy Warhol Museum | North Shore | Art | Over 4,000 Warhol art works in all media - paintings, drawings, prints, photographs, sculptures, installations, films |
| August Wilson Center for African American Culture | Cultural District | African American | Cultural center about African Americans in Western Pennsylvania and people of African descent around the world |
| Bicycle Heaven | Chateau | Transportation | website, bicycle store and museum |
| Carnegie Museum of Art | Oakland | Art | Contemporary art, hosts the Carnegie International every few years, sculpture, architectural drawings, photography |
| Carnegie Museum of Natural History | Oakland | Natural history | Dinosaurs, gems & minerals, Native American & Egyptian artifacts, nature dioramas, Powdermill Nature Reserve |
| Kamin Science Center | Chateau | Science | Hands-on exhibits, includes Miniature Railroad & Village of Western PA and the Highmark SportsWorks |
| Center for PostNatural History | Garfield | Natural history | Storefront museum, displays organisms that have been intentionally altered by humans by means including selective breeding or genetic engineering |
| Children's Museum of Pittsburgh | Allegheny Center | Children's | Exhibits include Mister Rogers' Neighborhood, Waterplay, Art Studio, Garage, Curiosity Lab, Theater, Nursery, artwork you can play with |
| The Clemente Museum | Lawrenceville | Biographical | Life of baseball Hall of Famer Roberto Clemente |
| Elmer H. Grimm Sr. Pharmacy Museum | Oakland | Medical | Historic pharmacy displays, part of the University of Pittsburgh |
| Fort Pitt Museum | Downtown | Open air | Fort Pitt's role during the French & Indian War, American Revolution, the Whiskey Rebellion and the founding of Pittsburgh, administered by the Pennsylvania Historical and Museum Commission |
| The Frick Pittsburgh | Point Breeze | Multiple | Includes Clayton, the restored Frick mansion; Frick Art Museum of fine and decorative art; Car and Carriage Museum with over 20 local antique cars; Greenhouse and grounds |
| Heinz History Center | Strip District | Local history | History of Western Pennsylvania, includes Western Pennsylvania Sports Museum; center also operates Meadowcroft Rockshelter |
| Kelso Museum of Near Eastern Archaeology | East Liberty | Archaeology | Operated by the Pittsburgh Theological Seminary, Bible archaeology, features collection of ancient Near Eastern and Palestinian pottery and artifacts |
| Mattress Factory | Central Northside | Art | Exhibits room-sized installation art |
| Miller Gallery at Carnegie Mellon University | Oakland | Art | Contemporary art gallery |
| Miniature Railroad & Village | Chateau | Model trains | Located in the Carnegie Science Center |
| Nationality Rooms | Oakland | Culture | Guided tours of special classrooms designed to celebrate a different culture that had an influence on Pittsburgh's growth; part of University of Pittsburgh |
| Photo Antiquities Museum of Photographic History | Allegheny Center | Art | website, history of photography, includes images, cameras and accessories |
| Pittsburgh Center for the Arts | Point Breeze | Art | Community arts campus that offers arts education programs and contemporary art exhibitions |
| Pittsburgh Glass Center | Garfield | Art | Hodge Gallery features contemporary glass |
| Randyland | Central Northside | Art | Dedicated to the outsider art of Randy Gilson. |
| Silver Eye Center for Photography | South Side Flats | Art | website, hosts four photography exhibits each year |
| Society for Contemporary Craft | Strip District | Art | Contemporary art in craft media by international, national and regional artists |
| Soldiers and Sailors Memorial Hall and Museum | Oakland | Military | Largest memorial in the United States dedicated solely to honoring all branches of military veterans and service personnel |
| Stephen Foster Memorial Museum | Oakland | Biographical | Collection of composer Stephen Foster, also two music theaters |
| ToonSeum | Cultural District | Art | Cartoon art |
| Trundle Manor | Swissvale | Art | Oddity museum dedicated to steampunk, horror, and classic film items. |
| University Arts Gallery | Oakland | Art | Part of the University of Pittsburgh |
| University of Pittsburgh Dental Museum | Oakland | Medical | Historic dental artifacts and equipment |
| Western Pennsylvania Sports Museum at the History Center | Strip District | Sports | Located in the Smithsonian wing of the Heinz History Center, focuses on sports history in the Pittsburgh region |
| Wood Street Galleries | Cultural District | Art | Contemporary art gallery featuring new media artists from around the globe |
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