MAP1LC3A

MAP1LC3A
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 3ECI, 3WAL, 3WAN, 4ZDV
Identifiers
Aliases	MAP1LC3A , ATG8E, LC3, LC3A, MAP1ALC3, MAP1BLC3, microtubule associated protein 1 light chain 3 alpha
External IDs	OMIM: 601242; MGI: 1915661; HomoloGene: 12021; GeneCards: MAP1LC3A; OMA:MAP1LC3A - orthologs
Gene location (Human) Chr. Chromosome 20 (human) [1] Band 20q11.22 Start 34,546,854 bp [1] End 34,560,345 bp [1]
Gene location (Mouse) Chr. Chromosome 2 (mouse) [2] Band 2|2 H1 Start 155,118,217 bp [2] End 155,119,993 bp [2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in right hemisphere of cerebellum right frontal lobe Brodmann area 9 cingulate gyrus anterior cingulate cortex prefrontal cortex hypothalamus C1 segment amygdala anterior pituitary Top expressed in lip muscle of thigh right ventricle dentate gyrus of hippocampal formation granule cell superior frontal gyrus primary visual cortex seminiferous tubule dorsomedial hypothalamic nucleus digastric muscle triceps brachii muscle More reference expression data BioGPS n/a
Gene ontology Molecular function phosphatidylethanolamine binding protein binding phospholipid binding ubiquitin protein ligase binding microtubule binding Cellular component cytoplasm organelle membrane cytosol late endosome membrane autophagosome microtubule cytoskeleton cytoplasmic vesicle endomembrane system autolysosome autophagosome membrane synapse Biological process autophagy autophagy of mitochondrion autophagosome assembly response to iron(II) ion response to lead ion response to nutrient levels cellular response to amino acid starvation response to morphine cellular response to hydrogen peroxide cellular response to copper ion cellular response to nitrogen starvation cellular response to starvation macroautophagy autophagosome maturation Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 84557 66734 Ensembl ENSG00000101460 ENSMUSG00000027602 UniProt Q9H492 Q91VR7 RefSeq (mRNA) NM_032514 NM_181509 NM_025735 RefSeq (protein) NP_115903 NP_852610 NP_080011 Location (UCSC) Chr 20: 34.55 – 34.56 Mb Chr 2: 155.12 – 155.12 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

Microtubule-associated proteins 1A/1B light chain 3A is a protein that in humans is encoded by the MAP1LC3A gene. ^{[5] [6] [7]} Two transcript variants encoding different isoforms have been found for this gene.

Function

MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. ^[7]

MAPLC3A is one of the mammalian homologues of yeast ATG8, an important marker and effector of autophagy. ^[8]

Regulation

MAP1LC3A is regulated by several post-translational modifications. These include covalent linkage of the C-terminus to phosphatidylethanolamine in autophagic membranes, and phosphorylation by protein kinase A, ^[9] which downregulates its autophagy functions. Noncovalent interactions are important for its cargo targeting functions in selective autophagy. For example, it has been shown to interact with sequestosome 1. ^[10]

References

1. GRCh38: Ensembl release 89: ENSG00000101460 – Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000027602 – Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Mann SS, Hammarback JA (May 1997). "Gene localization and developmental expression of light chain 3: a common subunit of microtubule-associated protein 1A(MAP1A) and MAP1B". J Neurosci Res. 43 (5): 535–44. doi:10.1002/(SICI)1097-4547(19960301)43:5<535::AID-JNR3>3.0.CO;2-J. PMID   8833088. S2CID   23515321.
6. Pankiv S, Clausen TH, Lamark T, Brech A, Bruun JA, Outzen H, Overvatn A, Bjorkoy G, Johansen T (Aug 2007). "p62/SQSTM1 binds directly to Atg8/LC3 to facilitate degradation of ubiquitinated protein aggregates by autophagy". J Biol Chem. 282 (33): 24131–45. doi: 10.1074/jbc.M702824200 . PMID   17580304.
7. "Entrez Gene: MAP1LC3A microtubule-associated protein 1 light chain 3 alpha".
8. Valente G, Morani F, Nicotra G, Fusco N, Peracchio C, Titone R, Alabiso O, Arisio R, Katsaros D, Benedetto C, Isidoro C (2014). "Expression and clinical significance of the autophagy proteins BECLIN 1 and LC3 in ovarian cancer". Biomed Res Int. 2014 462658. doi: 10.1155/2014/462658 . PMC   4127242 . PMID   25136588.
9. Cherra SJ, Kulich SM, Uechi G, Balasubramani M, Mountzouris J, Day BW, Chu CT (August 2010). "Regulation of the autophagy protein LC3 by phosphorylation". J. Cell Biol. 190 (4): 533–9. doi:10.1083/jcb.201002108. PMC   2928022 . PMID   20713600.
10. Shvets E, Fass E, Scherz-Shouval R, Elazar Z (August 2008). "The N-terminus and Phe52 residue of LC3 recruit p62/SQSTM1 into autophagosomes". J. Cell Sci. 121 (Pt 16): 2685–95. doi: 10.1242/jcs.026005 . PMID   18653543.

Further reading

• Snásel J, Pichová I (1997). "The cleavage of host cell proteins by HIV-1 protease". Folia Biol. (Praha). 42 (5): 227–30. doi:10.1007/BF02818986. PMID   8997639. S2CID   7617882.
• Wallin M, Deinum J, Goobar L, Danielson UH (1990). "Proteolytic cleavage of microtubule-associated proteins by retroviral proteinases". J. Gen. Virol. 71 (9): 1985–91. doi: 10.1099/0022-1317-71-9-1985 . PMID   2212989.
• Schoenfeld TA, McKerracher L, Obar R, Vallee RB (1989). "MAP 1A and MAP 1B are structurally related microtubule associated proteins with distinct developmental patterns in the CNS". J. Neurosci. 9 (5): 1712–30. doi: 10.1523/JNEUROSCI.09-05-01712.1989 . PMC   6569839 . PMID   2470876.
• Mann SS, Hammarback JA (1994). "Molecular characterization of light chain 3. A microtubule binding subunit of MAP1A and MAP1B". J. Biol. Chem. 269 (15): 11492–7. doi: 10.1016/S0021-9258(19)78150-2 . PMID   7908909.
• Hartley JL, Temple GF, Brasch MA (2001). "DNA Cloning Using In Vitro Site-Specific Recombination". Genome Res. 10 (11): 1788–95. doi:10.1101/gr.143000. PMC   310948 . PMID   11076863.
• Wiemann S, Weil B, Wellenreuther R, et al. (2001). "Toward a Catalog of Human Genes and Proteins: Sequencing and Analysis of 500 Novel Complete Protein Coding Human cDNAs". Genome Res. 11 (3): 422–35. doi:10.1101/gr.GR1547R. PMC   311072 . PMID   11230166.
• Bonnet C, Boucher D, Lazereg S, et al. (2001). "Differential binding regulation of microtubule-associated proteins MAP1A, MAP1B, and MAP2 by tubulin polyglutamylation". J. Biol. Chem. 276 (16): 12839–48. doi: 10.1074/jbc.M011380200 . PMID   11278895.
• Deloukas P, Matthews LH, Ashurst J, et al. (2002). "The DNA sequence and comparative analysis of human chromosome 20". Nature. 414 (6866): 865–71. Bibcode:2001Natur.414..865D. doi: 10.1038/414865a . PMID   11780052.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi: 10.1073/pnas.242603899 . PMC   139241 . PMID   12477932.
• He H, Dang Y, Dai F, et al. (2003). "Post-translational modifications of three members of the human MAP1LC3 family and detection of a novel type of modification for MAP1LC3B". J. Biol. Chem. 278 (31): 29278–87. doi: 10.1074/jbc.M303800200 . PMID   12740394.
• Tanida I, Sou YS, Ezaki J, et al. (2004). "HsAtg4B/HsApg4B/autophagin-1 cleaves the carboxyl termini of three human Atg8 homologues and delipidates microtubule-associated protein light chain 3- and GABAA receptor-associated protein-phospholipid conjugates". J. Biol. Chem. 279 (35): 36268–76. doi: 10.1074/jbc.M401461200 . PMID   15187094.
• Kouno T, Mizuguchi M, Tanida I, et al. (2005). "1H, 13C, and 15N resonance assignments of human microtubule-associated protein light chain-3". J. Biomol. NMR. 29 (3): 415–6. doi:10.1023/B:JNMR.0000032505.99071.ea. PMID   15213446. S2CID   23561733.
• Goehler H, Lalowski M, Stelzl U, et al. (2004). "A protein interaction network links GIT1, an enhancer of huntingtin aggregation, to Huntington's disease". Mol. Cell. 15 (6): 853–65. doi: 10.1016/j.molcel.2004.09.016 . PMID   15383276.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC   528928 . PMID   15489334.
• Wiemann S, Arlt D, Huber W, et al. (2004). "From ORFeome to Biology: A Functional Genomics Pipeline". Genome Res. 14 (10B): 2136–44. doi:10.1101/gr.2576704. PMC   528930 . PMID   15489336.
• Kouno T, Mizuguchi M, Tanida I, et al. (2005). "Solution structure of microtubule-associated protein light chain 3 and identification of its functional subdomains". J. Biol. Chem. 280 (26): 24610–7. doi: 10.1074/jbc.M413565200 . PMID   15857831.
• Sou YS, Tanida I, Komatsu M, et al. (2006). "Phosphatidylserine in addition to phosphatidylethanolamine is an in vitro target of the mammalian Atg8 modifiers, LC3, GABARAP, and GATE-16". J. Biol. Chem. 281 (6): 3017–24. doi: 10.1074/jbc.M505888200 . PMID   16303767.
• Mehrle A, Rosenfelder H, Schupp I, et al. (2006). "The LIFEdb database in 2006". Nucleic Acids Res. 34 (Database issue): D415–8. doi:10.1093/nar/gkj139. PMC   1347501 . PMID   16381901.