Myopalladin

MYPN
Identifiers
Aliases	MYPN , CMD1DD, CMH22, MYOP, RCM4, myopalladin, NEM11
External IDs	OMIM: 608517; MGI: 1916052; HomoloGene: 23778; GeneCards: MYPN; OMA:MYPN - orthologs
Gene location (Human) Chr. Chromosome 10 (human) [1] Band 10q21.3 Start 68,087,897 bp [1] End 68,212,017 bp [1]
Gene location (Mouse) Chr. Chromosome 10 (mouse) [2] Band 10|10 B4 Start 62,951,574 bp [2] End 63,039,731 bp [2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in muscle of thigh gastrocnemius muscle vastus lateralis muscle Skeletal muscle tissue of rectus abdominis deltoid muscle tibialis anterior muscle Skeletal muscle tissue of biceps brachii myocardium of left ventricle apex of heart right auricle of heart Top expressed in medial head of gastrocnemius muscle knee joint triceps brachii muscle extraocular muscle vastus lateralis muscle ankle temporal muscle digastric muscle sternocleidomastoid muscle tibialis anterior muscle More reference expression data BioGPS n/a
Gene ontology Molecular function SH3 domain binding cytoskeletal protein binding actin binding protein binding muscle alpha-actinin binding actin filament binding cell-cell adhesion mediator activity Cellular component cytoplasm sarcomere Z discdkac I band nucleus plasma membrane axon Biological process sarcomere organization regulation of cell migration regulation of cytoskeleton organization homophilic cell adhesion via plasma membrane adhesion molecules axon guidance dendrite self-avoidance Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 84665 68802 Ensembl ENSG00000138347 ENSMUSG00000020067 UniProt Q86TC9 Q5DTJ9 RefSeq (mRNA) NM_001256267 NM_001256268 NM_032578 NM_182992 RefSeq (protein) NP_001243196 NP_001243197 NP_115967 NP_892037 Location (UCSC) Chr 10: 68.09 – 68.21 Mb Chr 10: 62.95 – 63.04 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

Myopalladin is a protein that in humans is encoded by the MYPN gene. Myopalladin is a muscle protein responsible for tethering proteins at the Z-disc and for communicating between the sarcomere and the nucleus in cardiac and skeletal muscle ^{[5] [6] [7]}

Structure

Myopalladin is a 145.2 kDa protein composed of 1320 amino acids. ^{[8] [9]} Myopalladin has five Ig-like repeats within the protein, and a proline-rich domain. Myopalladin binds the Src homology domain of nebulette and nebulin and tethers it to alpha-actinin via its C-terminal domain binding to the EF hand domains of alpha-actinin. The N-terminal region of myopalladin binds to the nuclear protein CARP, known to regulate gene expression in muscle. ^[5] It also has been shown to bind ANKRD23. ^[10]

Function

Myopalladin has dual subcellular localization, residing in both the nucleus and sarcomere/I-bands in muscle. Accordingly, myopalladin has functions in both sarcomere assembly and in control of gene expression. ^[5] Specifics of these functions were gleaned from studies involving MYPN mutants associated with various cardiomyopathies. The Q529X myopalladin mutant demonstrated incompetence in recruiting key binding partners such as desmin, alpha-actinin and CARP to the Z-disc during myofibrilogenesis. In contrast, the Y20C mutant resulted in decreased expression of binding partners. ^[11]

Clinical significance

Mutations in MYPN have been linked to nemaline myopathy, dilated cardiomyopathy, hypertrophic cardiomyopathy and restrictive cardiomyopathy. ^{[11] [12] [13]}

References

1. GRCh38: Ensembl release 89: ENSG00000138347 – Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000020067 – Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Bang ML, Mudry RE, McElhinny AS, Trombitás K, Geach AJ, Yamasaki R, Sorimachi H, Granzier H, Gregorio CC, Labeit S (April 2001). "Myopalladin, a novel 145-kilodalton sarcomeric protein with multiple roles in Z-disc and I-band protein assemblies". The Journal of Cell Biology. 153 (2): 413–27. doi:10.1083/jcb.153.2.413. PMC   2169455 . PMID   11309420.
6. Ma K, Wang K (December 2002). "Interaction of nebulin SH3 domain with titin PEVK and myopalladin: implications for the signaling and assembly role of titin and nebulin". FEBS Letters. 532 (3): 273–8. Bibcode:2002FEBSL.532..273M. doi: 10.1016/S0014-5793(02)03655-4 . PMID   12482578. S2CID   18125444.
7. "Entrez Gene: MYPN myopalladin".
8. Chung, Joon-Sub. "Protein Information - Myopalladin". Cardiac Organellar Protein Atlas Knowledgebase (COPaKB). NHLBI Proteomics Center at UCLA. Retrieved 2015-04-29.
9. Zong NC, Li H, Li H, Lam MP, Jimenez RC, Kim CS, Deng N, Kim AK, Choi JH, Zelaya I, Liem D, Meyer D, Odeberg J, Fang C, Lu HJ, Xu T, Weiss J, Duan H, Uhlen M, Yates JR, Apweiler R, Ge J, Hermjakob H, Ping P (October 2013). "Integration of cardiac proteome biology and medicine by a specialized knowledgebase". Circulation Research. 113 (9): 1043–53. doi:10.1161/CIRCRESAHA.113.301151. PMC   4076475 . PMID   23965338.
10. Miller MK, Bang ML, Witt CC, Labeit D, Trombitas C, Watanabe K, Granzier H, McElhinny AS, Gregorio CC, Labeit S (November 2003). "The muscle ankyrin repeat proteins: CARP, ankrd2/Arpp and DARP as a family of titin filament-based stress response molecules". Journal of Molecular Biology. 333 (5): 951–64. doi:10.1016/j.jmb.2003.09.012. PMID   14583192.
11. Purevjav E, Arimura T, Augustin S, Huby AC, Takagi K, Nunoda S, Kearney DL, Taylor MD, Terasaki F, Bos JM, Ommen SR, Shibata H, Takahashi M, Itoh-Satoh M, McKenna WJ, Murphy RT, Labeit S, Yamanaka Y, Machida N, Park JE, Alexander PM, Weintraub RG, Kitaura Y, Ackerman MJ, Kimura A, Towbin JA (May 2012). "Molecular basis for clinical heterogeneity in inherited cardiomyopathies due to myopalladin mutations". Human Molecular Genetics. 21 (9): 2039–53. doi:10.1093/hmg/dds022. PMC   3315208 . PMID   22286171.
12. Duboscq-Bidot L, Xu P, Charron P, Neyroud N, Dilanian G, Millaire A, Bors V, Komajda M, Villard E (January 2008). "Mutations in the Z-band protein myopalladin gene and idiopathic dilated cardiomyopathy". Cardiovascular Research. 77 (1): 118–25. doi: 10.1093/cvr/cvm015 . PMID   18006477.
13. Laitila, J, Wallgren, C (2021). "Recent advances in nemaline myopathy". Neuromuscular Disorders. 31 (10): 955–967. doi: 10.1016/j.nmd.2021.07.012 . hdl: 10138/337407 . PMID   34561123.

Further reading

• Hartley JL, Temple GF, Brasch MA (November 2000). "DNA cloning using in vitro site-specific recombination". Genome Research. 10 (11): 1788–95. doi:10.1101/gr.143000. PMC   310948 . PMID   11076863.
• Wiemann S, Weil B, Wellenreuther R, Gassenhuber J, Glassl S, Ansorge W, Böcher M, Blöcker H, Bauersachs S, Blum H, Lauber J, Düsterhöft A, Beyer A, Köhrer K, Strack N, Mewes HW, Ottenwälder B, Obermaier B, Tampe J, Heubner D, Wambutt R, Korn B, Klein M, Poustka A (March 2001). "Toward a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs". Genome Research. 11 (3): 422–35. doi:10.1101/gr.GR1547R. PMC   311072 . PMID   11230166.
• Miller MK, Bang ML, Witt CC, Labeit D, Trombitas C, Watanabe K, Granzier H, McElhinny AS, Gregorio CC, Labeit S (November 2003). "The muscle ankyrin repeat proteins: CARP, ankrd2/Arpp and DARP as a family of titin filament-based stress response molecules". Journal of Molecular Biology. 333 (5): 951–64. doi:10.1016/j.jmb.2003.09.012. PMID   14583192.
• Wiemann S, Arlt D, Huber W, Wellenreuther R, Schleeger S, Mehrle A, Bechtel S, Sauermann M, Korf U, Pepperkok R, Sültmann H, Poustka A (October 2004). "From ORFeome to biology: a functional genomics pipeline". Genome Research. 14 (10B): 2136–44. doi:10.1101/gr.2576704. PMC   528930 . PMID   15489336.
• Mehrle A, Rosenfelder H, Schupp I, del Val C, Arlt D, Hahne F, Bechtel S, Simpson J, Hofmann O, Hide W, Glatting KH, Huber W, Pepperkok R, Poustka A, Wiemann S (January 2006). "The LIFEdb database in 2006". Nucleic Acids Research. 34 (Database issue): D415–8. doi:10.1093/nar/gkj139. PMC   1347501 . PMID   16381901.
• Beausoleil SA, Villén J, Gerber SA, Rush J, Gygi SP (October 2006). "A probability-based approach for high-throughput protein phosphorylation analysis and site localization". Nature Biotechnology. 24 (10): 1285–92. doi:10.1038/nbt1240. PMID   16964243. S2CID   14294292.