Nucleobindin 1

NUCB1
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 1SNL
Identifiers
Aliases	NUCB1 , CALNUC, NUC, nucleobindin 1
External IDs	OMIM: 601323 MGI: 97388 HomoloGene: 4507 GeneCards: NUCB1
Gene location (Human) Chr. Chromosome 19 (human) [1] Band 19q13.33 Start 48,900,312 bp [1] End 48,923,372 bp [1]
Gene location (Mouse) Chr. Chromosome 7 (mouse) [2] Band 7|7 B3 Start 45,139,882 bp [2] End 45,159,830 bp [2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in stromal cell of endometrium right lobe of liver ascending aorta left coronary artery right coronary artery right lobe of thyroid gland upper lobe of left lung popliteal artery left lobe of thyroid gland body of stomach Top expressed in external carotid artery internal carotid artery lip calvaria large intestine colon ileum duodenum islet of Langerhans molar More reference expression data BioGPS More reference expression data
Gene ontology Molecular function DNA binding calcium ion binding metal ion binding protein binding G-protein alpha-subunit binding Cellular component cytoplasm lumenal side of Golgi membrane rough endoplasmic reticulum membrane cis-Golgi network Golgi-associated vesicle Golgi cisterna membrane extracellular region extrinsic component of Golgi membrane trans-Golgi network early endosome extracellular exosome nucleus endoplasmic reticulum-Golgi intermediate compartment extracellular space Golgi apparatus endoplasmic reticulum lumen Biological process regulation of protein targeting response to cisplatin post-translational protein modification Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 4924 18220 Ensembl ENSG00000104805 ENSMUSG00000030824 UniProt Q02818 Q02819 RefSeq (mRNA) NM_006184 NM_001163662 NM_008749 RefSeq (protein) NP_006175 NP_001157134 NP_032775 Location (UCSC) Chr 19: 48.9 – 48.92 Mb Chr 7: 45.14 – 45.16 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

Nucleobindin-1 (NUCB1), also known as calnuc, is a protein that in humans is encoded by the NUCB1 gene. ^{[5] [6]}

Structure

The human calnuc protein contains 461 amino acids and has a pI of 4.9. ^[7]

The protein contains the following regions and domains:

• Signal peptide ^[8]
• Leucine-zipper ^[8] The leucine-zipper of calnuc may be responsible for its dimerization.
• aa 347-389 – Nuclear localization signal
• 2 zinc binding sites ^[9] with high affinity (K_d = 20 nM)
• carboxypeptidase-like motifs : HFREXnH (aa 66) and HFTEXnH (aa 146)
• 2 EF-hands
• Potential anchor region ^[7] – 15-aa hydrophobic region at its COOH terminus that may be sufficient for membrane anchoring

References

1. GRCh38: Ensembl release 89: ENSG00000104805 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000030824 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Miura K, Hirai M, Kanai Y, Kurosawa Y (Feb 1997). "Organization of the human gene for nucleobindin (NUC) and its chromosomal assignment to 19q13.2-q13.4". Genomics. 34 (2): 181–6. doi:10.1006/geno.1996.0263. PMID   8661046.
6. "Entrez Gene: NUCB1 nucleobindin 1".
7. Lin P, Le-Niculescu H, Hofmeister R, McCaffery JM, Jin M, Hennemann H, McQuistan T, De Vries L, Farquhar MG (June 1998). "The mammalian calcium-binding protein, nucleobindin (CALNUC), is a Golgi resident protein". J. Cell Biol. 141 (7): 1515–27. doi:10.1083/jcb.141.7.1515. PMC   2132997 . PMID   9647645.
8. Miura K, Titani K, Kurosawa Y, Kanai Y (August 1992). "Molecular cloning of nucleobindin, a novel DNA-binding protein that contains both a signal peptide and a leucine zipper structure". Biochem. Biophys. Res. Commun. 187 (1): 375–80. doi:10.1016/S0006-291X(05)81503-7. PMID   1520323.
9. Kanuru M, Raman R, Aradhyam GK (January 2013). "Serine protease activity of calnuc: regulation by Zn2+ and G proteins". J. Biol. Chem. 288 (3): 1762–73. doi: 10.1074/jbc.M112.382846 . PMC   3548486 . PMID   23195954.

Further reading

• Miura K, Titani K, Kurosawa Y, Kanai Y (1992). "Molecular cloning of nucleobindin, a novel DNA-binding protein that contains both a signal peptide and a leucine zipper structure". Biochem. Biophys. Res. Commun. 187 (1): 375–80. doi:10.1016/S0006-291X(05)81503-7. PMID   1520323.
• Mochizuki N, Hibi M, Kanai Y, Insel PA (1995). "Interaction of the protein nucleobindin with G alpha i2, as revealed by the yeast two-hybrid system". FEBS Lett. 373 (2): 155–8. doi: 10.1016/0014-5793(95)01031-9 . PMID   7589456. S2CID   84629575.
• Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID   8125298.
• Ballif BA, Mincek NV, Barratt JT, et al. (1996). "Interaction of cyclooxygenases with an apoptosis- and autoimmunity-associated protein". Proc. Natl. Acad. Sci. U.S.A. 93 (11): 5544–9. Bibcode:1996PNAS...93.5544B. doi: 10.1073/pnas.93.11.5544 . PMC   39283 . PMID   8643612.
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID   9373149.
• Kubota T, Miyauchi M, Miura K, et al. (1998). "Upregulation of nucleobindin expression in human-activated lymphocytes and non-Hodgkin's lymphoma". Pathol. Int. 48 (1): 22–8. doi:10.1111/j.1440-1827.1998.tb03823.x. PMID   9589460. S2CID   20338800.
• Lin P, Le-Niculescu H, Hofmeister R, et al. (1998). "The mammalian calcium-binding protein, nucleobindin (CALNUC), is a Golgi resident protein". J. Cell Biol. 141 (7): 1515–27. doi:10.1083/jcb.141.7.1515. PMC   2132997 . PMID   9647645.
• Taniguchi N, Taniura H, Niinobe M, et al. (2000). "The postmitotic growth suppressor necdin interacts with a calcium-binding protein (NEFA) in neuronal cytoplasm". J. Biol. Chem. 275 (41): 31674–81. doi: 10.1074/jbc.M005103200 . PMID   10915798.
• Weiss TS, Chamberlain CE, Takeda T, et al. (2002). "Galpha i3 binding to calnuc on Golgi membranes in living cells monitored by fluorescence resonance energy transfer of green fluorescent protein fusion proteins". Proc. Natl. Acad. Sci. U.S.A. 98 (26): 14961–6. doi: 10.1073/pnas.261572098 . PMC   64966 . PMID   11752444.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi: 10.1073/pnas.242603899 . PMC   139241 . PMID   12477932.
• Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi: 10.1038/ng1285 . PMID   14702039.
• de Alba E, Tjandra N (2004). "Structural studies on the Ca2+-binding domain of human nucleobindin (calnuc)". Biochemistry. 43 (31): 10039–49. doi:10.1021/bi049310a. PMID   15287731.
• Breuza L, Halbeisen R, Jenö P, et al. (2004). "Proteomics of endoplasmic reticulum-Golgi intermediate compartment (ERGIC) membranes from brefeldin A-treated HepG2 cells identifies ERGIC-32, a new cycling protein that interacts with human Erv46". J. Biol. Chem. 279 (45): 47242–53. doi: 10.1074/jbc.M406644200 . PMID   15308636.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC   528928 . PMID   15489334.
• Rual JF, Venkatesan K, Hao T, et al. (2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–8. Bibcode:2005Natur.437.1173R. doi:10.1038/nature04209. PMID   16189514. S2CID   4427026.
• Chen Y, Lin P, Qiu S, et al. (2007). "Autoantibodies to Ca2+ binding protein Calnuc is a potential marker in colon cancer detection" (PDF). Int. J. Oncol. 30 (5): 1137–44. doi: 10.3892/ijo.30.5.1137 . PMID   17390015.
• Tsukumo Y, Tomida A, Kitahara O, et al. (2007). "Nucleobindin 1 controls the unfolded protein response by inhibiting ATF6 activation". J. Biol. Chem. 282 (40): 29264–72. doi: 10.1074/jbc.M705038200 . PMID   17686766.