PCGF1

PCGF1
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 4HPL, 4HPM
Identifiers
Aliases	PCGF1 , 2010002K04Rik, NSPC1, RNF3A-2, RNF68, polycomb group ring finger 1
External IDs	OMIM: 610231; MGI: 1917087; HomoloGene: 13090; GeneCards: PCGF1; OMA:PCGF1 - orthologs
Gene location (Human) Chr. Chromosome 2 (human) [1] Band 2p13.1 Start 74,505,043 bp [1] End 74,507,695 bp [1]
Gene location (Mouse) Chr. Chromosome 6 (mouse) [2] Band 6 C3|6 35.94 cM Start 83,054,850 bp [2] End 83,057,836 bp [2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in oocyte secondary oocyte granulocyte mucosa of transverse colon right lobe of liver right hemisphere of cerebellum pituitary gland body of stomach body of pancreas right uterine tube Top expressed in secondary oocyte zygote primary oocyte adrenal gland genital tubercle bone marrow morula neural layer of retina olfactory bulb pancreas More reference expression data BioGPS More reference expression data
Gene ontology Molecular function protein C-terminus binding protein binding metal ion binding promoter-specific chromatin binding Cellular component PcG protein complex nucleus nucleoplasm PRC1 complex Biological process histone H2A monoubiquitination transcription, DNA-templated regulation of transcription, DNA-templated histone H2A-K119 monoubiquitination negative regulation of transcription by RNA polymerase II Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 84759 69837 Ensembl ENSG00000115289 ENSMUSG00000069678 UniProt Q9BSM1 Q8R023 RefSeq (mRNA) NM_032673 NM_197992 RefSeq (protein) NP_116062 NP_932109 NP_001390487 NP_001390488 NP_001390489 NP_001390490 Location (UCSC) Chr 2: 74.51 – 74.51 Mb Chr 6: 83.05 – 83.06 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

Polycomb group RING finger protein 1, PCGF1, also known as NSPC1 or RNF68 is a RING finger domain protein that in humans is encoded by the PCGF1 gene. ^{[5] [6]}

PCGF1 is a component defining the non-canonical polycomb repressive complex 1.1 (ncPRC1) interacting with RING1A/B, RYBP, BCOR and KDM2B. ^[7] PCGF1-BCOR assembles via the ubiquitin-like RAWUL domain of PCGF1 and is recruited on the chromatin at KDM2B sites. ^{[8] [9] [10]} Within the PRC1-like complex, PCGF1 regulates RING1B ubiquitin ligase activity that catalyzes the ubiquitination of Lys119 on histone H2A, ^[11] which then leads to recruitment of PRC2 and H3K27me3 to effectively initiate a polycomb domain and mediate gene repression.  ^{[12] [13] [14]}

References

1. GRCh38: Ensembl release 89: ENSG00000115289 – Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000069678 – Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Nunes M, Blanc I, Maes J, Fellous M, Robert B, McElreavey K (April 2001). "NSPc1, a novel mammalian Polycomb gene, is expressed in neural crest-derived structures of the peripheral nervous system". Mechanisms of Development. 102 (1–2): 219–22. doi: 10.1016/S0925-4773(01)00288-X . PMID   11287196. S2CID   8344430.
6. "Entrez Gene: PCGF1 polycomb group ring finger 1".
7. Gao Z, Zhang J, Bonasio R, Strino F, Sawai A, Parisi F, et al. (February 2012). "PCGF homologs, CBX proteins, and RYBP define functionally distinct PRC1 family complexes". Molecular Cell. 45 (3): 344–56. doi:10.1016/j.molcel.2012.01.002. PMC   3293217 . PMID   22325352.
8. Wong SJ, Gearhart MD, Taylor AB, Nanyes DR, Ha DJ, Robinson AK, et al. (October 2016). "KDM2B Recruitment of the Polycomb Group Complex, PRC1.1, Requires Cooperation between PCGF1 and BCORL1". Structure. 24 (10): 1795–1801. doi:10.1016/j.str.2016.07.011. PMC   5088048 . PMID   27568929.
9. Junco SE, Wang R, Gaipa JC, Taylor AB, Schirf V, Gearhart MD, et al. (April 2013). "Structure of the polycomb group protein PCGF1 in complex with BCOR reveals basis for binding selectivity of PCGF homologs". Structure. 21 (4): 665–71. doi:10.1016/j.str.2013.02.013. PMC   4003909 . PMID   23523425.
10. Farcas AM, Blackledge NP, Sudbery I, Long HK, McGouran JF, Rose NR, et al. (December 2012). Struhl K (ed.). "KDM2B links the Polycomb Repressive Complex 1 (PRC1) to recognition of CpG islands". eLife. 1: e00205. doi: 10.7554/eLife.00205 . PMC   3524939 . PMID   23256043.
11. Taherbhoy AM, Huang OW, Cochran AG (July 2015). "BMI1-RING1B is an autoinhibited RING E3 ubiquitin ligase". Nature Communications. 6: 7621. Bibcode:2015NatCo...6.7621T. doi: 10.1038/ncomms8621 . PMID   26151332.
12. Tamburri S, Lavarone E, Fernández-Pérez D, Conway E, Zanotti M, Manganaro D, Pasini D (February 2020). "Histone H2AK119 Mono-Ubiquitination Is Essential for Polycomb-Mediated Transcriptional Repression". Molecular Cell. 77 (4): 840–856.e5. doi:10.1016/j.molcel.2019.11.021. PMC   7033561 . PMID   31883952.
13. Fursova NA, Blackledge NP, Nakayama M, Ito S, Koseki Y, Farcas AM, et al. (June 2019). "Synergy between Variant PRC1 Complexes Defines Polycomb-Mediated Gene Repression". Molecular Cell. 74 (5): 1020–1036.e8. doi:10.1016/j.molcel.2019.03.024. PMC   6561741 . PMID   31029541.
14. Blackledge NP, Farcas AM, Kondo T, King HW, McGouran JF, Hanssen LL, et al. (June 2014). "Variant PRC1 complex-dependent H2A ubiquitylation drives PRC2 recruitment and polycomb domain formation". Cell. 157 (6): 1445–1459. doi:10.1016/j.cell.2014.05.004. PMC   4048464 . PMID   24856970.

Further reading

• Valk-Lingbeek ME, Bruggeman SW, van Lohuizen M (August 2004). "Stem cells and cancer; the polycomb connection". Cell. 118 (4): 409–18. doi: 10.1016/j.cell.2004.08.005 . PMID   15315754. S2CID   16542938.
• Gong Y, Yue J, Wu X, Wang X, Wen J, Lu L, et al. (2007). "NSPc1 is a cell growth regulator that acts as a transcriptional repressor of p21Waf1/Cip1 via the RARE element". Nucleic Acids Research. 34 (21): 6158–69. doi:10.1093/nar/gkl834. PMC   1693893 . PMID   17088287.
• Gearhart MD, Corcoran CM, Wamstad JA, Bardwell VJ (September 2006). "Polycomb group and SCF ubiquitin ligases are found in a novel BCOR complex that is recruited to BCL6 targets". Molecular and Cellular Biology. 26 (18): 6880–9. doi:10.1128/MCB.00630-06. PMC   1592854 . PMID   16943429.
• Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, et al. (October 2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–8. Bibcode:2005Natur.437.1173R. doi:10.1038/nature04209. PMID   16189514. S2CID   4427026.
• Gong Y, Wang X, Liu J, Shi L, Yin B, Peng X, et al. (January 2005). "NSPc1, a mainly nuclear localized protein of novel PcG family members, has a transcription repression activity related to its PKC phosphorylation site at S183". FEBS Letters. 579 (1): 115–21. doi: 10.1016/j.febslet.2004.11.056 . PMID   15620699. S2CID   28187618.
• Sugishita H, Kondo T, Ito S, et al. (September 2021). "Variant PCGF1-PRC1 links PRC2 recruitment with differentiation-associated transcriptional inactivation at target genes". Nat Commun. 12 (5341): 5341. Bibcode:2021NatCo..12.5341S. doi: 10.1038/s41467-021-24894-z . PMC   8429492 . PMID   34504070.