PEX14

PEX14
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 2W84, 2W85, 4BXU
Identifiers
Aliases	PEX14 , NAPP2, PBD13A, Pex14p, dJ734G22.2, peroxisomal biogenesis factor 14
External IDs	OMIM: 601791; MGI: 1927868; HomoloGene: 37936; GeneCards: PEX14; OMA:PEX14 - orthologs
Gene location (Human) Chr. Chromosome 1 (human) [1] Band 1p36.22 Start 10,472,288 bp [1] End 10,630,758 bp [1]
Gene location (Mouse) Chr. Chromosome 4 (mouse) [2] Band 4|4 E2 Start 149,044,992 bp [2] End 149,184,333 bp [2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in bronchial epithelial cell parotid gland right lobe of liver pancreatic ductal cell prefrontal cortex urinary bladder muscle of thigh olfactory zone of nasal mucosa endothelial cell oocyte Top expressed in otic placode saccule otic vesicle entorhinal cortex perirhinal cortex choroid plexus of fourth ventricle CA3 field Ileal epithelium lactiferous gland yolk sac More reference expression data BioGPS More reference expression data
Gene ontology Molecular function transcription corepressor activity protein N-terminus binding microtubule binding protein binding beta-tubulin binding signaling receptor binding Cellular component integral component of membrane membrane peroxisomal membrane peroxisome intracellular anatomical structure nucleus fibrillar center peroxisomal importomer complex protein-containing complex Biological process peroxisome organization negative regulation of protein homotetramerization peroxisome transport along microtubule negative regulation of protein binding negative regulation of DNA-binding transcription factor activity microtubule anchoring protein import into peroxisome matrix protein import into peroxisome matrix, substrate release protein transport negative regulation of transcription, DNA-templated protein homooligomerization protein import into peroxisome matrix, translocation transport protein targeting to peroxisome protein import into peroxisome matrix, docking protein ubiquitination protein-containing complex assembly Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 5195 56273 Ensembl ENSG00000142655 ENSMUSG00000028975 UniProt O75381 Q9R0A0 RefSeq (mRNA) NM_004565 NM_019781 RefSeq (protein) NP_004556 NP_062755 Location (UCSC) Chr 1: 10.47 – 10.63 Mb Chr 4: 149.04 – 149.18 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

Peroxisomal membrane anchor protein (Pex14p) conserved region
Identifiers
Symbol	Pex14_N
Pfam	PF04695
InterPro	IPR006785
Available protein structures: Pfam   structures / ECOD   PDB RCSB PDB; PDBe; PDBj PDBsum structure summary

Peroxisomal membrane protein PEX14 is a protein that in humans is encoded by the PEX14 gene. ^{[5] [6]}

Function

This gene encodes an essential component of the peroxisomal import machinery. The protein is integrated into peroxisome membranes with its C-terminus exposed to the cytosol, and interacts with the cytosolic receptor for proteins containing a PTS1 peroxisomal targeting signal. The protein also functions as a transcriptional corepressor and interacts with a histone deacetylase. A mutation in this gene results in one form of Zellweger syndrome. ^[6]

Interactions

PEX14 has been shown to interact with

• PEX5, ^{[7] [8] [9] [10]}
• PEX7, ^[10] and
• PEX13. ^[7]

References

1. GRCh38: Ensembl release 89: ENSG00000142655 – Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000028975 – Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Fransen M, Terlecky SR, Subramani S (Jul 1998). "Identification of a human PTS1 receptor docking protein directly required for peroxisomal protein import". Proceedings of the National Academy of Sciences of the United States of America. 95 (14): 8087–92. Bibcode:1998PNAS...95.8087F. doi: 10.1073/pnas.95.14.8087 . PMC   20933 . PMID   9653144.
6. "Entrez Gene: PEX14 peroxisomal biogenesis factor 14".
7. Otera H, Setoguchi K, Hamasaki M, Kumashiro T, Shimizu N, Fujiki Y (Mar 2002). "Peroxisomal targeting signal receptor Pex5p interacts with cargoes and import machinery components in a spatiotemporally differentiated manner: conserved Pex5p WXXXF/Y motifs are critical for matrix protein import". Molecular and Cellular Biology. 22 (6): 1639–55. doi:10.1128/MCB.22.6.1639-1655.2002. PMC   135613 . PMID   11865044.
8. Okumoto K, Abe I, Fujiki Y (Aug 2000). "Molecular anatomy of the peroxin Pex12p: ring finger domain is essential for Pex12p function and interacts with the peroxisome-targeting signal type 1-receptor Pex5p and a ring peroxin, Pex10p". The Journal of Biological Chemistry. 275 (33): 25700–10. doi: 10.1074/jbc.M003303200 . PMID   10837480.
9. Shimizu N, Itoh R, Hirono Y, Otera H, Ghaedi K, Tateishi K, Tamura S, Okumoto K, Harano T, Mukai S, Fujiki Y (Apr 1999). "The peroxin Pex14p. cDNA cloning by functional complementation on a Chinese hamster ovary cell mutant, characterization, and functional analysis". The Journal of Biological Chemistry. 274 (18): 12593–604. doi: 10.1074/jbc.274.18.12593 . PMID   10212238.
10. Managadze D, Würtz C, Sichting M, Niehaus G, Veenhuis M, Rottensteiner H (Jun 2007). "The peroxin PEX14 of Neurospora crassa is essential for the biogenesis of both glyoxysomes and Woronin bodies" (PDF). Traffic. 8 (6): 687–701. doi: 10.1111/j.1600-0854.2007.00560.x . PMID   17461798. S2CID   221250.

Further reading

• Albertini M, Rehling P, Erdmann R, Girzalsky W, Kiel JA, Veenhuis M, Kunau WH (Apr 1997). "Pex14p, a peroxisomal membrane protein binding both receptors of the two PTS-dependent import pathways" (PDF). Cell. 89 (1): 83–92. doi:10.1016/S0092-8674(00)80185-3. PMID   9094717. S2CID   18878596.
• Huhse B, Rehling P, Albertini M, Blank L, Meller K, Kunau WH (Jan 1998). "Pex17p of Saccharomyces cerevisiae is a novel peroxin and component of the peroxisomal protein translocation machinery". The Journal of Cell Biology. 140 (1): 49–60. doi:10.1083/jcb.140.1.49. PMC   2132588 . PMID   9425153.
• Will GK, Soukupova M, Hong X, Erdmann KS, Kiel JA, Dodt G, Kunau WH, Erdmann R (Mar 1999). "Identification and characterization of the human orthologue of yeast Pex14p". Molecular and Cellular Biology. 19 (3): 2265–77. doi:10.1128/mcb.19.3.2265. PMC   84019 . PMID   10022913.
• Girzalsky W, Rehling P, Stein K, Kipper J, Blank L, Kunau WH, Erdmann R (Mar 1999). "Involvement of Pex13p in Pex14p localization and peroxisomal targeting signal 2-dependent protein import into peroxisomes". The Journal of Cell Biology. 144 (6): 1151–62. doi:10.1083/jcb.144.6.1151. PMC   2150583 . PMID   10087260.
• Shimizu N, Itoh R, Hirono Y, Otera H, Ghaedi K, Tateishi K, Tamura S, Okumoto K, Harano T, Mukai S, Fujiki Y (Apr 1999). "The peroxin Pex14p. cDNA cloning by functional complementation on a Chinese hamster ovary cell mutant, characterization, and functional analysis". The Journal of Biological Chemistry. 274 (18): 12593–604. doi: 10.1074/jbc.274.18.12593 . PMID   10212238.
• Sacksteder KA, Jones JM, South ST, Li X, Liu Y, Gould SJ (Mar 2000). "PEX19 binds multiple peroxisomal membrane proteins, is predominantly cytoplasmic, and is required for peroxisome membrane synthesis". The Journal of Cell Biology. 148 (5): 931–44. doi:10.1083/jcb.148.5.931. PMC   2174547 . PMID   10704444.
• Gavva NR, Wen SC, Daftari P, Moniwa M, Yang WM, Yang-Feng LP, Seto E, Davie JR, Shen CK (Mar 2002). "NAPP2, a peroxisomal membrane protein, is also a transcriptional corepressor". Genomics. 79 (3): 423–31. doi:10.1006/geno.2002.6714. PMID   11863372.
• Otera H, Setoguchi K, Hamasaki M, Kumashiro T, Shimizu N, Fujiki Y (Mar 2002). "Peroxisomal targeting signal receptor Pex5p interacts with cargoes and import machinery components in a spatiotemporally differentiated manner: conserved Pex5p WXXXF/Y motifs are critical for matrix protein import". Molecular and Cellular Biology. 22 (6): 1639–55. doi:10.1128/MCB.22.6.1639-1655.2002. PMC   135613 . PMID   11865044.
• Alland L, David G, Shen-Li H, Potes J, Muhle R, Lee HC, Hou H, Chen K, DePinho RA (Apr 2002). "Identification of mammalian Sds3 as an integral component of the Sin3/histone deacetylase corepressor complex". Molecular and Cellular Biology. 22 (8): 2743–50. doi:10.1128/MCB.22.8.2743-2750.2002. PMC   133736 . PMID   11909966.
• Fransen M, Brees C, Ghys K, Amery L, Mannaerts GP, Ladant D, Van Veldhoven PP (Mar 2002). "Analysis of mammalian peroxin interactions using a non-transcription-based bacterial two-hybrid assay". Molecular & Cellular Proteomics. 1 (3): 243–52. doi: 10.1074/mcp.M100025-MCP200 . PMID   12096124.
• Oliveira ME, Reguenga C, Gouveia AM, Guimarães CP, Schliebs W, Kunau WH, Silva MT, Sá-Miranda C, Azevedo JE (Dec 2002). "Mammalian Pex14p: membrane topology and characterisation of the Pex14p-Pex14p interaction". Biochimica et Biophysica Acta (BBA) - Biomembranes. 1567 (1–2): 13–22. doi: 10.1016/s0005-2736(02)00635-1 . PMID   12488033.
• Fransen M, Vastiau I, Brees C, Brys V, Mannaerts GP, Van Veldhoven PP (Mar 2004). "Potential role for Pex19p in assembly of PTS-receptor docking complexes". The Journal of Biological Chemistry. 279 (13): 12615–24. doi: 10.1074/jbc.M304941200 . PMID   14715663.
• Shimozawa N, Tsukamoto T, Nagase T, Takemoto Y, Koyama N, Suzuki Y, Komori M, Osumi T, Jeannette G, Wanders RJ, Kondo N (Jun 2004). "Identification of a new complementation group of the peroxisome biogenesis disorders and PEX14 as the mutated gene". Human Mutation. 23 (6): 552–8. doi: 10.1002/humu.20032 . PMID   15146459. S2CID   6324241.
• Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, Berriz GF, Gibbons FD, Dreze M, Ayivi-Guedehoussou N, Klitgord N, Simon C, Boxem M, Milstein S, Rosenberg J, Goldberg DS, Zhang LV, Wong SL, Franklin G, Li S, Albala JS, Lim J, Fraughton C, Llamosas E, Cevik S, Bex C, Lamesch P, Sikorski RS, Vandenhaute J, Zoghbi HY, Smolyar A, Bosak S, Sequerra R, Doucette-Stamm L, Cusick ME, Hill DE, Roth FP, Vidal M (Oct 2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–8. Bibcode:2005Natur.437.1173R. doi:10.1038/nature04209. PMID   16189514. S2CID   4427026.
• Kimura K, Wakamatsu A, Suzuki Y, Ota T, Nishikawa T, Yamashita R, Yamamoto J, Sekine M, Tsuritani K, Wakaguri H, Ishii S, Sugiyama T, Saito K, Isono Y, Irie R, Kushida N, Yoneyama T, Otsuka R, Kanda K, Yokoi T, Kondo H, Wagatsuma M, Murakawa K, Ishida S, Ishibashi T, Takahashi-Fujii A, Tanase T, Nagai K, Kikuchi H, Nakai K, Isogai T, Sugano S (Jan 2006). "Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes". Genome Research. 16 (1): 55–65. doi:10.1101/gr.4039406. PMC   1356129 . PMID   16344560.
• Nguyen T, Bjorkman J, Paton BC, Crane DI (Feb 2006). "Failure of microtubule-mediated peroxisome division and trafficking in disorders with reduced peroxisome abundance". Journal of Cell Science. 119 (Pt 4): 636–45. doi: 10.1242/jcs.02776 . hdl: 2440/23242 . PMID   16449325.

External links

• GeneReviews/NCBI/NIH/UW entry on Peroxisome Biogenesis Disorders, Zellweger Syndrome Spectrum
• OMIM entries on Peroxisome Biogenesis Disorders, Zellweger Syndrome Spectrum
• Eukaryotic Linear Motif resource motif class TRG_PEX_1
• Eukaryotic Linear Motif resource motif class TRG_PEX_2
• Overview of all the structural information available in the PDB for UniProt : O75381 (Peroxisomal membrane protein PEX14) at the PDBe-KB .