POU2AF1

POU2AF1
Available structures
PDB	Ortholog search: PDBe RCSB
List of PDB id codes
	1CQT
Identifiers
Aliases	POU2AF1 , BOB1, OBF-1, OBF1, OCAB, POU class 2 associating factor 1, POU class 2 homeobox associating factor 1
External IDs	OMIM: 601206 MGI: 105086 HomoloGene: 4543 GeneCards: POU2AF1
Gene location (Human)
Chr.	Chromosome 11 (human)
End	111,455,630 bp
Gene location (Mouse)
Chr.	Chromosome 9 (mouse)
End	51,151,380 bp
RNA expression pattern
	Top expressed in
	pylorus; ; cardia; ; spleen; ; lymph node; ; appendix; ; bronchial epithelial cell; ; thymus; ; bone marrow cells; ; spongy bone; ; duodenum;
	Top expressed in
	spleen; ; submandibular gland; ; blood; ; female urethra; ; subcutaneous adipose tissue; ; duodenum; ; bone marrow; ; thymus; ; Paneth cell; ; detrusor urinae muscle;
	More reference expression data
	More reference expression data
Gene ontology
Molecular function	DNA binding ; transcription coactivator activity ; protein binding ; transcription coregulator activity ; RNA polymerase II core promoter sequence-specific DNA binding ; RNA polymerase II cis-regulatory region sequence-specific DNA binding ;
Cellular component	nucleus ; RNA polymerase II transcription regulator complex ;
Biological process	regulation of transcription, DNA-templated ; humoral immune response ; transcription by RNA polymerase II ; transcription, DNA-templated ; positive regulation of transcription by RNA polymerase II ;
	Sources:Amigo / QuickGO
Orthologs
	5450
	18985
	ENSG00000110777
	ENSMUSG00000032053
	Q16633
	Q64693
	NM_006235
	NM_011136
	NP_006226
	NP_035266
	Wikidata
View/Edit Human	View/Edit Mouse

Last updated January 03, 2024

POU domain class 2-associating factor 1 is a protein that in humans is encoded by the POU2AF1 gene.^[5]^[6] The protein is also termed Oct coactivator from B cells (i.e. OCAB), Oct binding factor 1 (OBF1 or OBF-1), and, as commonly found in the literature, BOB1.^[7] BOB1 is a transcriptional coactivator (i.e. a protein that controls the activity of transcription factors) which is expressed principally by B-cell lymphocytes and controls immunoglobulin and other genes critical for these cells expression of CD20, CRISP-3, and CD36.^[8] The expression of BOB1 has proven useful for identifying certain lymphomas as being B-cell lymphomas,^[9] as exemplified in studies which use BOB1 expression to help identify lymphomas as being diffuse large B-cell lymphomas, not otherwise specified.^[10]

Interactions

POU2AF1 has been shown to interact with:

POU2F1 ^[11]^[12]^[13] and
SIAH1.^[14]^[15]

Related Research Articles

Oct-4, also known as POU5F1, is a protein that in humans is encoded by the POU5F1 gene. Oct-4 is a homeodomain transcription factor of the POU family. It is critically involved in the self-renewal of undifferentiated embryonic stem cells. As such, it is frequently used as a marker for undifferentiated cells. Oct-4 expression must be closely regulated; too much or too little will cause differentiation of the cells.

Robert G. Roeder is an American biochemist. He is known as a pioneer scientist in eukaryotic transcription. He discovered three distinct nuclear RNA polymerases in 1969 and characterized many proteins involved in the regulation of transcription, including basic transcription factors and the first mammalian gene-specific activator over five decades of research. He is the recipient of the Gairdner Foundation International Award in 2000, the Albert Lasker Award for Basic Medical Research in 2003, and the Kyoto Prize in 2021. He currently serves as Arnold and Mabel Beckman Professor and Head of the Laboratory of Biochemical and Molecular Biology at The Rockefeller University.

Octamer transcription factors are a family of transcription factors which binds to the "ATTTGCAT" DNA sequence. Their DNA-binding domain is a POU domain.

CIITA is a human gene which encodes a protein called the class II, major histocompatibility complex, transactivator. Mutations in this gene are responsible for the bare lymphocyte syndrome in which the immune system is severely compromised and cannot effectively fight infection. Chromosomal rearrangement of CIITA is involved in the pathogenesis of Hodgkin lymphoma and primary mediastinal B cell lymphoma.

Transcription factor p65 also known as nuclear factor NF-kappa-B p65 subunit is a protein that in humans is encoded by the RELA gene.

TEAD2, together with TEAD1, defines a novel family of transcription factors, the TEAD family, highly conserved through evolution. TEAD proteins were notably found in Drosophila (Scalloped), C. elegans, S. cerevisiae and A. nidulans. TEAD2 has been less studied than TEAD1 but a few studies revealed its role during development.

POU domain, class 2, transcription factor 1 is a protein that in humans is encoded by the POU2F1 gene.

E3 ubiquitin-protein ligase SIAH1 is an enzyme that in humans is encoded by the SIAH1 gene.

Four and a half LIM domains protein 2 also known as FHL-2 is a protein that in humans is encoded by the FHL2 gene. LIM proteins contain a highly conserved double zinc finger motif called the LIM domain.

Non-POU domain-containing octamer-binding protein (NonO) is a protein that in humans is encoded by the NONO gene.

POU is a family of eukaryotic transcription factors that have well-conserved homeodomains. The Pou domain is a bipartite DNA binding domain found in these proteins.

Host cell factor 1, also known as VP16-accessory protein, is a protein that in humans is encoded by the HCFC1 gene.

Paired box protein Pax-5 is a protein that in humans is encoded by the PAX5 gene.

Solute carrier family 22 member 1 is a protein that in humans is encoded by the gene SLC22A1.

POU domain, class 3, transcription factor 2 is a protein that in humans is encoded by the POU3F2 gene.

POU domain, class 4, transcription factor 1 (POU4F1) also known as brain-specific homeobox/POU domain protein 3A (BRN3A), homeobox/POU domain protein RDC-1 or Oct-T1 is a protein that in humans is encoded by the POU4F1 gene.

Zinc finger protein Aiolos also known as Ikaros family zinc finger protein 3 is a protein that in humans is encoded by the IKZF3 gene.

Myocyte enhancer binding factor 2B (MEF2B) is a transcription factor part of the MEF2 gene family including MEF2A, MEF2C, and MEF2D. However, MEF2B is distant from the other three branches of MEF2 genes as it lacks the protein-coding Holliday junction recognition protein C-terminal (HJURP_C) region in vertebrates.

POU domain, class 3, transcription factor 1 is a protein that in humans is encoded by the POU3F1 gene.

Transcription initiation factor TFIID subunit 4B is a protein that in humans is encoded by the TAF4B gene.

References

1 2 3 GRCh38: Ensembl release 89: ENSG00000110777 - Ensembl, May 2017
1 2 3 GRCm38: Ensembl release 89: ENSMUSG00000032053 - Ensembl, May 2017
↑ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
↑ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
↑ Junker S, Brøndum-Nielsen K, Newell JW, Matthias P, Tommerup N (Jun 1996). "Assignment of the human gene for Oct-binding factor-1 (OBF1), a B-cell-specific coactivator of octamer-binding transcription factors 1 and 2, to 11q23.1 by somatic cell hybridization and in situ hybridization". Genomics. 33 (1): 143–5. doi:10.1006/geno.1996.0173. PMID 8617501.
↑ "Entrez Gene: POU2AF1 POU domain, class 2, associating factor 1".
↑ "POU2AF1 POU class 2 homeobox associating factor 1 [ Homo sapiens (human) ]". Gene. National Library of Medicine, National Center for Biotechnology Information. 23 November 2023. Retrieved 2 January 2024.
↑ Ponzoni M, Arrigoni G, Doglioni C (January 2007). "New transcription factors in diagnostic hematopathology". Advances in Anatomic Pathology. 14 (1): 25–35. doi:10.1097/PAP.0b013e31802f0495. PMID 17198308. S2CID 37644377.
↑ Yin L, Xu J, Li M, Reddy V, Zhou Q, Liu H, Chu P, Zhang Q, Huang Q, Gao Z, Liang X, Wang HY, Pan Z (November 2016). "Oct2 and Bob1 are sensitive and specific markers in lineage determination of B cell lymphomas with no expression of conventional B cell markers". Histopathology. 69 (5): 775–783. doi:10.1111/his.13017. PMID 27319306. S2CID 12613073.
↑ Grimm KE, O'Malley DP (February 2019). "Aggressive B cell lymphomas in the 2017 revised WHO classification of tumors of hematopoietic and lymphoid tissues". Annals of Diagnostic Pathology. 38: 6–10. doi:10.1016/j.anndiagpath.2018.09.014. PMID 30380402. S2CID 53196244.
↑ Chasman D, Cepek K, Sharp PA, Pabo CO (Oct 1999). "Crystal structure of an OCA-B peptide bound to an Oct-1 POU domain/octamer DNA complex: specific recognition of a protein-DNA interface". Genes Dev. 13 (20): 2650–7. doi:10.1101/gad.13.20.2650. PMC 317104 . PMID 10541551.
↑ Wong MW, Henry RW, Ma B, Kobayashi R, Klages N, Matthias P, Strubin M, Hernandez N (Jan 1998). "The large subunit of basal transcription factor SNAPc is a Myb domain protein that interacts with Oct-1". Mol. Cell. Biol. 18 (1): 368–77. doi:10.1128/mcb.18.1.368. PMC 121507 . PMID 9418884.
↑ Lee L, Stollar E, Chang J, Grossmann JG, O'Brien R, Ladbury J, Carpenter B, Roberts S, Luisi B (Jun 2001). "Expression of the Oct-1 transcription factor and characterization of its interactions with the Bob1 coactivator". Biochemistry. 40 (22): 6580–8. doi:10.1021/bi010095x. PMID 11380252.
↑ Tiedt R, Bartholdy BA, Matthias G, Newell JW, Matthias P (Aug 2001). "The RING finger protein Siah-1 regulates the level of the transcriptional coactivator OBF-1". EMBO J. 20 (15): 4143–52. doi:10.1093/emboj/20.15.4143. PMC 149178 . PMID 11483517.
↑ Jahn L, Hombrink P, Hagedoorn RS, Kester MG, van der Steen DM, Rodriguez T, Pentcheva-Hoang T, de Ru AH, Schoonakker MP, Meeuwsen MH, Griffioen M, van Veelen PA, Falkenburg JH, Heemskerk MH (March 2017). "TCR-based therapy for multiple myeloma and other B-cell malignancies targeting intracellular transcription factor BOB1". Blood. 129 (10): 1284–1295. doi: 10.1182/blood-2016-09-737536 . PMID 28053195.

Teitell MA (2004). "OCA-B regulation of B-cell development and function". Trends Immunol. 24 (10): 546–53. doi:10.1016/j.it.2003.08.002. PMID 14552839.
Luo Y, Roeder RG (1995). "Cloning, functional characterization, and mechanism of action of the B-cell-specific transcriptional coactivator OCA-B". Mol. Cell. Biol. 15 (8): 4115–24. doi:10.1128/MCB.15.8.4115. PMC 230650 . PMID 7623806.
Gstaiger M, Knoepfel L, Georgiev O, Schaffner W, Hovens CM (1995). "A B-cell coactivator of octamer-binding transcription factors". Nature. 373 (6512): 360–2. Bibcode:1995Natur.373..360G. doi:10.1038/373360a0. PMID 7779176. S2CID 4303288.
Strubin M, Newell JW, Matthias P (1995). "OBF-1, a novel B cell-specific coactivator that stimulates immunoglobulin promoter activity through association with octamer-binding proteins". Cell. 80 (3): 497–506. doi: 10.1016/0092-8674(95)90500-6 . PMID 7859290. S2CID 6139601.
Galiègue-Zouitina S, Quief S, Hildebrand MP, Denis C, Lecocq G, Collyn-d'Hooghe M, Bastard C, Yuille M, Dyer MJ, Kerckaert JP (1996). "Fusion of the LAZ3/BCL6 and BOB1/OBF1 genes by t(3; 11) (q27; q23) chromosomal translocation". Comptes Rendus de l'Académie des Sciences, Série III. 318 (11): 1125–31. PMID 8574789.
Zwilling S, Dieckmann A, Pfisterer P, Angel P, Wirth T (1997). "Inducible expression and phosphorylation of coactivator BOB.1/OBF.1 in T cells". Science. 277 (5323): 221–5. doi:10.1126/science.277.5323.221. PMID 9211847.
Sauter P, Matthias P (1998). "The B cell-specific coactivator OBF-1 (OCA-B, Bob-1) is inducible in T cells and its expression is dispensable for IL-2 gene induction". Immunobiology. 198 (1–3): 207–16. doi:10.1016/S0171-2985(97)80041-1. PMID 9442392.
Luo Y, Ge H, Stevens S, Xiao H, Roeder RG (1998). "Coactivation by OCA-B: Definition of Critical Regions and Synergism with General Cofactors". Mol. Cell. Biol. 18 (7): 3803–10. doi:10.1128/mcb.18.7.3803. PMC 108964 . PMID 9632764.
Sauter P, Matthias P (1998). "Coactivator OBF-1 Makes Selective Contacts with Both the POU-Specific Domain and the POU Homeodomain and Acts as a Molecular Clamp on DNA". Mol. Cell. Biol. 18 (12): 7397–409. doi:10.1128/MCB.18.12.7397. PMC 109321 . PMID 9819426.
Chasman D, Cepek K, Sharp PA, Pabo CO (1999). "Crystal structure of an OCA-B peptide bound to an Oct-1 POU domain/octamer DNA complex: specific recognition of a protein-DNA interface". Genes Dev. 13 (20): 2650–7. doi:10.1101/gad.13.20.2650. PMC 317104 . PMID 10541551.
Tomilin A, Reményi A, Lins K, Bak H, Leidel S, Vriend G, Wilmanns M, Schöler HR (2001). "Synergism with the coactivator OBF-1 (OCA-B, BOB-1) is mediated by a specific POU dimer configuration". Cell. 103 (6): 853–64. doi: 10.1016/S0092-8674(00)00189-6 . PMID 11136971. S2CID 14650955.
Lee L, Stollar E, Chang J, Grossmann JG, O'Brien R, Ladbury J, Carpenter B, Roberts S, Luisi B (2001). "Expression of the Oct-1 transcription factor and characterization of its interactions with the Bob1 coactivator". Biochemistry. 40 (22): 6580–8. doi:10.1021/bi010095x. PMID 11380252.
Tiedt R, Bartholdy BA, Matthias G, Newell JW, Matthias P (2001). "The RING finger protein Siah-1 regulates the level of the transcriptional coactivator OBF-1". EMBO J. 20 (15): 4143–52. doi:10.1093/emboj/20.15.4143. PMC 149178 . PMID 11483517.
Boehm J, He Y, Greiner A, Staudt L, Wirth T (2001). "Regulation of BOB.1/OBF.1 stability by SIAH". EMBO J. 20 (15): 4153–62. doi:10.1093/emboj/20.15.4153. PMC 149152 . PMID 11483518.
Malone CS, Wall R (2002). "Bob1 (OCA-B/OBF-1) differential transactivation of the B cell-specific B29 (Ig beta) and mb-1 (Ig alpha) promoters". J. Immunol. 168 (7): 3369–75. doi: 10.4049/jimmunol.168.7.3369 . PMID 11907094.
Casellas R, Jankovic M, Meyer G, Gazumyan A, Luo Y, Roeder R, Nussenzweig M (2003). "OcaB is required for normal transcription and V(D)J recombination of a subset of immunoglobulin kappa genes". Cell. 110 (5): 575–85. doi: 10.1016/S0092-8674(02)00911-X . PMID 12230975. S2CID 17887117.
Schaffer A, Kim EC, Wu X, Zan H, Testoni L, Salamon S, Cerutti A, Casali P (2003). "Selective inhibition of class switching to IgG and IgE by recruitment of the HoxC4 and Oct-1 homeodomain proteins and Ku70/Ku86 to newly identified ATTT cis-elements". J. Biol. Chem. 278 (25): 23141–50. doi: 10.1074/jbc.M212952200 . PMID 12672812.
Lins K, Reményi A, Tomilin A, Massa S, Wilmanns M, Matthias P, Schöler HR (2003). "OBF1 enhances transcriptional potential of Oct1". EMBO J. 22 (9): 2188–98. doi:10.1093/emboj/cdg199. PMC 156071 . PMID 12727885.

This article on a gene on human chromosome 11 is a stub. You can help Wikipedia by expanding it.

This page is based on this Wikipedia article
Text is available under the CC BY-SA 4.0 license; additional terms may apply.
Images, videos and audio are available under their respective licenses.

[refGRCh38Ensembl-1] 1 2 3 GRCh38: Ensembl release 89: ENSG00000110777 - Ensembl, May 2017

[refGRCm38Ensembl-2] 1 2 3 GRCm38: Ensembl release 89: ENSMUSG00000032053 - Ensembl, May 2017

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[pmid8617501-5] Junker S, Brøndum-Nielsen K, Newell JW, Matthias P, Tommerup N (Jun 1996). "Assignment of the human gene for Oct-binding factor-1 (OBF1), a B-cell-specific coactivator of octamer-binding transcription factors 1 and 2, to 11q23.1 by somatic cell hybridization and in situ hybridization". Genomics. 33 (1): 143–5. doi:10.1006/geno.1996.0173. PMID 8617501.

[entrez-6] "Entrez Gene: POU2AF1 POU domain, class 2, associating factor 1".

[7] "POU2AF1 POU class 2 homeobox associating factor 1 [ Homo sapiens (human) ]". Gene. National Library of Medicine, National Center for Biotechnology Information. 23 November 2023. Retrieved 2 January 2024.

[pmid17198308-8] Ponzoni M, Arrigoni G, Doglioni C (January 2007). "New transcription factors in diagnostic hematopathology". Advances in Anatomic Pathology. 14 (1): 25–35. doi:10.1097/PAP.0b013e31802f0495. PMID 17198308. S2CID 37644377.

[pmid27319306-9] Yin L, Xu J, Li M, Reddy V, Zhou Q, Liu H, Chu P, Zhang Q, Huang Q, Gao Z, Liang X, Wang HY, Pan Z (November 2016). "Oct2 and Bob1 are sensitive and specific markers in lineage determination of B cell lymphomas with no expression of conventional B cell markers". Histopathology. 69 (5): 775–783. doi:10.1111/his.13017. PMID 27319306. S2CID 12613073.

[pmid30380402-10] Grimm KE, O'Malley DP (February 2019). "Aggressive B cell lymphomas in the 2017 revised WHO classification of tumors of hematopoietic and lymphoid tissues". Annals of Diagnostic Pathology. 38: 6–10. doi:10.1016/j.anndiagpath.2018.09.014. PMID 30380402. S2CID 53196244.

[pmid10541551-11] Chasman D, Cepek K, Sharp PA, Pabo CO (Oct 1999). "Crystal structure of an OCA-B peptide bound to an Oct-1 POU domain/octamer DNA complex: specific recognition of a protein-DNA interface". Genes Dev. 13 (20): 2650–7. doi:10.1101/gad.13.20.2650. PMC 317104 . PMID 10541551.

[pmid9418884-12] Wong MW, Henry RW, Ma B, Kobayashi R, Klages N, Matthias P, Strubin M, Hernandez N (Jan 1998). "The large subunit of basal transcription factor SNAPc is a Myb domain protein that interacts with Oct-1". Mol. Cell. Biol. 18 (1): 368–77. doi:10.1128/mcb.18.1.368. PMC 121507 . PMID 9418884.

[pmid11380252-13] Lee L, Stollar E, Chang J, Grossmann JG, O'Brien R, Ladbury J, Carpenter B, Roberts S, Luisi B (Jun 2001). "Expression of the Oct-1 transcription factor and characterization of its interactions with the Bob1 coactivator". Biochemistry. 40 (22): 6580–8. doi:10.1021/bi010095x. PMID 11380252.

[pmid11483517-14] Tiedt R, Bartholdy BA, Matthias G, Newell JW, Matthias P (Aug 2001). "The RING finger protein Siah-1 regulates the level of the transcriptional coactivator OBF-1". EMBO J. 20 (15): 4143–52. doi:10.1093/emboj/20.15.4143. PMC 149178 . PMID 11483517.

[pmid28053195-15] Jahn L, Hombrink P, Hagedoorn RS, Kester MG, van der Steen DM, Rodriguez T, Pentcheva-Hoang T, de Ru AH, Schoonakker MP, Meeuwsen MH, Griffioen M, van Veelen PA, Falkenburg JH, Heemskerk MH (March 2017). "TCR-based therapy for multiple myeloma and other B-cell malignancies targeting intracellular transcription factor BOB1". Blood. 129 (10): 1284–1295. doi: 10.1182/blood-2016-09-737536 . PMID 28053195.

[1]

[2]

[3]

[4]

[5]

[6]

[7]

[8]

[9]

[10]

[11]

[12]

[13]

[14]

[15]

POU2AF1

Contents

Interactions

See also

Related Research Articles

References

Further reading