A protosteroid or primordial fat [1] is a lipid precursor, which can be transformed during subsequent biochemical reactions and finally become steroid. [2] The protosteroids are biomarkers that are produced by ancient eukaryotes belonged to the microorganisms in the protosterol biota. The intermediate compounds created by these eukaryotes while making crown sterol molecules. [3]
For the first time, the German biochemist and Nobel laureate Konrad Emil Bloch thought that instead of today's sterols, earlier life forms could have used chemical intermediates in their cells. He called these intermediates protosteroids. [4] Later researchers synthesized protosteroids called lanosterol, cycloartenol, and 24-methylene cycloartenol. Then researchers from the Australian National University and the University of Bremen [5] found protosteroids in rocks that formed 1.6 billion years ago in the Barney Creek Formation in Northern Australia. The researchers also found derivatives that matched the pattern produced by 24-methylene cycloartenol in 1.3-billion-year-old rocks. [6]
The Precambrian is the earliest part of Earth's history, set before the current Phanerozoic Eon. The Precambrian is so named because it preceded the Cambrian, the first period of the Phanerozoic Eon, which is named after Cambria, the Latinised name for Wales, where rocks from this age were first studied. The Precambrian accounts for 88% of the Earth's geologic time.
The Proterozoic is a geological eon spanning the time interval from 2500 to 538.8 million years ago. It is the most recent part of the Precambrian "supereon". It is also the longest eon of the Earth's geologic time scale, and it is subdivided into three geologic eras : the Paleoproterozoic, Mesoproterozoic, and Neoproterozoic.
A steroid is a biologically active organic compound with four rings arranged in a specific molecular configuration. Steroids have two principal biological functions: as important components of cell membranes that alter membrane fluidity; and as signaling molecules. Hundreds of steroids are found in plants, animals and fungi. All steroids are manufactured in cells from the sterols lanosterol (opisthokonts) or cycloartenol (plants). Lanosterol and cycloartenol are derived from the cyclization of the triterpene squalene.
A unicellular organism, also known as a single-celled organism, is an organism that consists of a single cell, unlike a multicellular organism that consists of multiple cells. Organisms fall into two general categories: prokaryotic organisms and eukaryotic organisms. Most prokaryotes are unicellular and are classified into bacteria and archaea. Many eukaryotes are multicellular, but some are unicellular such as protozoa, unicellular algae, and unicellular fungi. Unicellular organisms are thought to be the oldest form of life, with early protocells possibly emerging 3.8–4.0 billion years ago.
Hopanoids are a diverse subclass of triterpenoids with the same hydrocarbon skeleton as the compound hopane. This group of pentacyclic molecules therefore refers to simple hopenes, hopanols and hopanes, but also to extensively functionalized derivatives such as bacteriohopanepolyols (BHPs) and hopanoids covalently attached to lipid A.
Lanosterol is a tetracyclic triterpenoid and is the compound from which all animal and fungal steroids are derived. By contrast plant steroids are produced via cycloartenol.
Sterane (cyclopentanoperhydrophenanthrenes) compounds are a class of tetracyclic triterpanes derived from steroids or sterols via diagenetic and catagenetic degradation, such as hydrogenation. Steranes are detected in sediments and sedimentary rocks in nature. Steranes have an androstane skeleton with a side chain at carbon C-17. The sterane structure constitutes the core of all sterols. Steranes are widely used as biomarkers for the presence of eukaryotes in past ecosystems because steroids are nearly exclusively produced by eukaryotes. In particular, cholestanes are diagenetic products of cholesterol in animals, while stigmastanes are diagenetic products of stigmasterols in algae and land plants. However, some bacteria are now known to produce sterols and it is inferred that the ultimate origin of sterol biosynthesis is in bacteria. Sterols are produced via protosterols that are direct cyclization compounds of squalene by the catalysis of oxidosqualene cyclase. All known sterols in eukaryotes are enzymatically extensively modified from protosterols, while organisms that only produce protosterols are not known. The oldest record of modified steranes are in sedimentary rocks deposited ca. 720–820 million years ago. In contrast, diagenetic products of protosterols are widely distributed in older Proterozoic rocks and imply the presence of extinct proto-eukaryotes and/or sterol-producing bacteria before the evolution of crown-group eukaryotes.
β-sitosterol (beta-sitosterol) is one of several phytosterols with chemical structures similar to that of cholesterol. It is a white, waxy powder with a characteristic odor, and is one of the components of the food additive E499. Phytosterols are hydrophobic and soluble in alcohols.
Vaalbara was an Archean supercontinent consisting of the Kaapvaal Craton and the Pilbara Craton. E. S. Cheney derived the name from the last four letters of each craton's name. The two cratons consist of crust dating from 2.7 to 3.6 Gya, which would make Vaalbara one of Earth's earliest supercontinents.
The Ediacaranbiota is a taxonomic period classification that consists of all life forms that were present on Earth during the Ediacaran Period. These were enigmatic tubular and frond-shaped, mostly sessile, organisms. Trace fossils of these organisms have been found worldwide, and represent the earliest known complex multicellular organisms. The term "Ediacara biota" has received criticism from some scientists due to its alleged inconsistency, arbitrary exclusion of certain fossils, and inability to be precisely defined.
Lanosterol synthase is an oxidosqualene cyclase (OSC) enzyme that converts (S)-2,3-oxidosqualene to a protosterol cation and finally to lanosterol. Lanosterol is a key four-ringed intermediate in cholesterol biosynthesis. In humans, lanosterol synthase is encoded by the LSS gene.
The history of life on Earth traces the processes by which living and fossil organisms evolved, from the earliest emergence of life to present day. Earth formed about 4.5 billion years ago and evidence suggests that life emerged prior to 3.7 Ga. Although there is some evidence of life as early as 4.1 to 4.28 Ga, it remains controversial due to the possible non-biological formation of the purported fossils.
A microbial mat is a multi-layered sheet of microorganisms, mainly bacteria and archaea, or bacteria alone. Microbial mats grow at interfaces between different types of material, mostly on submerged or moist surfaces, but a few survive in deserts. A few are found as endosymbionts of animals.
The eukaryotes constitute the domain of Eukaryota, organisms whose cells have a nucleus. All animals, plants, fungi, and many unicellular organisms are eukaryotes. They constitute a major group of life forms, alongside the two groups of prokaryotes, the Bacteria and the Archaea. Eukaryotes represent a small minority of the number of organisms, but due to their generally much larger size, their collective global biomass is about equal to that of prokaryotes.
The Boring Billion, otherwise known as the Mid Proterozoic and Earth's Middle Ages, is the time period between 1.8 and 0.8 billion years ago (Ga) spanning the middle Proterozoic eon, characterized by more or less tectonic stability, climatic stasis, and slow biological evolution. It is bordered by two different oxygenation and glacial events, but the Boring Billion itself had very low oxygen levels and no evidence of glaciation.
Martin David Brasier FGS, FLS was an English palaeobiologist and astrobiologist known for his conceptual analysis of microfossils and evolution in the Precambrian and Cambrian.
The Francevillian biota are a collection of 2.1-billion-year-old Palaeoproterozoic macroscopic structures, controversially suggested to be fossils, known from the Francevillian B Formation in Gabon, a black shale province notable for its lack of any noticeable metamorphism. The structures have been postulated by some authors to be evidence of the earliest form of multicellular life, and of eukaryotes. They were discovered by an international team led by Moroccan-French geologist Abderrazak El Albani, of the University of Poitiers, France. While they have yet to be assigned to a formal taxonomic position, they have been informally and collectively referred to as the "Gabonionta", including by the Natural History Museum Vienna in 2014. The status of the structures as fossils has been questioned.
The earliest known life forms on Earth are believed to be fossilized microorganisms found in hydrothermal vent precipitates, considered to be about 3.42 billion years old. The earliest time for the origin of life on Earth is at least 3.77 billion years ago, possibly as early as 4.28 billion years ago—not long after the oceans formed 4.5 billion years ago, and after the formation of the Earth 4.54 billion years ago. The earliest direct evidence of life on Earth is from microfossils of microorganisms permineralized in 3.465-billion-year-old Australian Apex chert rocks, although the validity of these microfossils is debated.
The evolution of bacteria has progressed over billions of years since the Precambrian time with their first major divergence from the archaeal/eukaryotic lineage roughly 3.2-3.5 billion years ago. This was discovered through gene sequencing of bacterial nucleoids to reconstruct their phylogeny. Furthermore, evidence of permineralized microfossils of early prokaryotes was also discovered in the Australian Apex Chert rocks, dating back roughly 3.5 billion years ago during the time period known as the Precambrian time. This suggests that an organism in of the phylum Thermotogota was the most recent common ancestor of modern bacteria.
The protosterol biota is a group of organisms found in fossilized fats that comprised aquatic protosterol-producing bacteria and ancient deep-branching stem-group eukaryotes from 1.6 to 0.8 billion years ago. These organisms were more complex than today's bacteria and they predate the last common ancestors of all modern eukaryotes. Perhaps they hunted their own species, since they were predators, as they fed on other bacteria. They were present in large numbers in the aquatic environments of the seas and seriously affected the Earth's ecosystem at the time. These microorganisms adapted to the much lower oxygen levels of the era and are also thought to have produced protosteroids.