RFC5

RFC5
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 1LFS
Identifiers
Aliases	RFC5 , RFC36, replication factor C subunit 5
External IDs	OMIM: 600407; MGI: 1919401; HomoloGene: 6730; GeneCards: RFC5; OMA:RFC5 - orthologs
Gene location (Human) Chr. Chromosome 12 (human) [1] Band 12q24.23 Start 118,013,588 bp [1] End 118,033,130 bp [1]
Gene location (Mouse) Chr. Chromosome 5 (mouse) [2] Band 5|5 F Start 117,516,168 bp [2] End 117,527,112 bp [2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in pons superior vestibular nucleus body of tongue internal globus pallidus Skeletal muscle tissue of biceps brachii endothelial cell inferior ganglion of vagus nerve Brodmann area 23 subthalamic nucleus right ventricle Top expressed in epiblast embryo bone marrow embryo yolk sac thymus ventricular zone tail of embryo neural tube mesencephalon More reference expression data BioGPS More reference expression data
Gene ontology Molecular function nucleotide binding DNA binding single-stranded DNA helicase activity protein binding ATP binding enzyme binding DNA clamp loader activity Cellular component DNA replication factor C complex Ctf18 RFC-like complex nucleoplasm nucleus Biological process nucleotide-excision repair, DNA gap filling error-free translesion synthesis error-prone translesion synthesis positive regulation of DNA-directed DNA polymerase activity translesion synthesis transcription-coupled nucleotide-excision repair nucleotide-excision repair, DNA incision nucleotide-excision repair, DNA incision, 5'-to lesion DNA replication DNA repair telomere maintenance via semi-conservative replication DNA-dependent DNA replication regulation of signal transduction by p53 class mediator Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 5985 72151 Ensembl ENSG00000111445 ENSMUSG00000029363 UniProt P40937 Q9D0F6 RefSeq (mRNA) NM_001130112 NM_001130113 NM_001206801 NM_007370 NM_181578 NM_001346815 NM_028128 RefSeq (protein) NP_001123584 NP_001123585 NP_001193730 NP_001333744 NP_031396 NP_853556 NP_082404 Location (UCSC) Chr 12: 118.01 – 118.03 Mb Chr 5: 117.52 – 117.53 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

Replication factor C subunit 5 is a protein that in humans is encoded by the RFC5 gene. ^{[5] [6]}

Function

The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 36 kD subunit. This subunit can interact with the C-terminal region of PCNA. It forms a core complex with the 38 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding distinct isoforms have been reported. ^[6]

Interactions

RFC5 has been shown to interact with:

• BRD4, ^[7]
• CHTF18, ^[8]
• PCNA, ^{[9] [10] [11]}
• RFC2, ^{[9] [12]} and
• RFC4. ^{[9] [12] [13] [14]}

References

1. GRCh38: Ensembl release 89: ENSG00000111445 – Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000029363 – Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Okumura K, Nogami M, Taguchi H, Dean FB, Chen M, Pan ZQ, Hurwitz J, Shiratori A, Murakami Y, Ozawa K (Jan 1995). "Assignment of the 36.5-kDa (RFC5), 37-kDa (RFC4), 38-kDa (RFC3), and 40-kDa (RFC2) subunit genes of human replication factor C to chromosome bands 12q24.2-q24.3, 3q27, 13q12.3-q13, and 7q11.23". Genomics. 25 (1): 274–8. doi:10.1016/0888-7543(95)80135-9. PMID   7774928.
6. "Entrez Gene: RFC5 replication factor C (activator 1) 5, 36.5kDa".
7. Maruyama T, Farina A, Dey A, Cheong J, Bermudez VP, Tamura T, Sciortino S, Shuman J, Hurwitz J, Ozato K (Sep 2002). "A Mammalian bromodomain protein, brd4, interacts with replication factor C and inhibits progression to S phase". Molecular and Cellular Biology. 22 (18): 6509–20. doi:10.1128/mcb.22.18.6509-6520.2002. PMC   135621 . PMID   12192049.
8. Bermudez VP, Maniwa Y, Tappin I, Ozato K, Yokomori K, Hurwitz J (Sep 2003). "The alternative Ctf18-Dcc1-Ctf8-replication factor C complex required for sister chromatid cohesion loads proliferating cell nuclear antigen onto DNA". Proceedings of the National Academy of Sciences of the United States of America. 100 (18): 10237–42. Bibcode:2003PNAS..10010237B. doi: 10.1073/pnas.1434308100 . PMC   193545 . PMID   12930902.
9. Cai J, Gibbs E, Uhlmann F, Phillips B, Yao N, O'Donnell M, Hurwitz J (Jul 1997). "A complex consisting of human replication factor C p40, p37, and p36 subunits is a DNA-dependent ATPase and an intermediate in the assembly of the holoenzyme". The Journal of Biological Chemistry. 272 (30): 18974–81. doi: 10.1074/jbc.272.30.18974 . PMID   9228079.
10. Mossi R, Jónsson ZO, Allen BL, Hardin SH, Hübscher U (Jan 1997). "Replication factor C interacts with the C-terminal side of proliferating cell nuclear antigen". The Journal of Biological Chemistry. 272 (3): 1769–76. doi: 10.1074/jbc.272.3.1769 . PMID   8999859.
11. Ohta S, Shiomi Y, Sugimoto K, Obuse C, Tsurimoto T (Oct 2002). "A proteomics approach to identify proliferating cell nuclear antigen (PCNA)-binding proteins in human cell lysates. Identification of the human CHL12/RFCs2-5 complex as a novel PCNA-binding protein". The Journal of Biological Chemistry. 277 (43): 40362–7. doi: 10.1074/jbc.M206194200 . PMID   12171929.
12. Cai J, Yao N, Gibbs E, Finkelstein J, Phillips B, O'Donnell M, Hurwitz J (Sep 1998). "ATP hydrolysis catalyzed by human replication factor C requires participation of multiple subunits". Proceedings of the National Academy of Sciences of the United States of America. 95 (20): 11607–12. Bibcode:1998PNAS...9511607C. doi: 10.1073/pnas.95.20.11607 . PMC   21688 . PMID   9751713.
13. Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, Berriz GF, Gibbons FD, Dreze M, Ayivi-Guedehoussou N, Klitgord N, Simon C, Boxem M, Milstein S, Rosenberg J, Goldberg DS, Zhang LV, Wong SL, Franklin G, Li S, Albala JS, Lim J, Fraughton C, Llamosas E, Cevik S, Bex C, Lamesch P, Sikorski RS, Vandenhaute J, Zoghbi HY, Smolyar A, Bosak S, Sequerra R, Doucette-Stamm L, Cusick ME, Hill DE, Roth FP, Vidal M (Oct 2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–8. Bibcode:2005Natur.437.1173R. doi:10.1038/nature04209. PMID   16189514. S2CID   4427026.
14. Ellison V, Stillman B (Mar 1998). "Reconstitution of recombinant human replication factor C (RFC) and identification of an RFC subcomplex possessing DNA-dependent ATPase activity". The Journal of Biological Chemistry. 273 (10): 5979–87. doi: 10.1074/jbc.273.10.5979 . PMID   9488738.

Further reading

• O'Donnell M, Onrust R, Dean FB, Chen M, Hurwitz J (Jan 1993). "Homology in accessory proteins of replicative polymerases--E. coli to humans". Nucleic Acids Research. 21 (1): 1–3. doi:10.1093/nar/21.1.1. PMC   309057 . PMID   8441605.
• Uhlmann F, Cai J, Flores-Rozas H, Dean FB, Finkelstein J, O'Donnell M, Hurwitz J (Jun 1996). "In vitro reconstitution of human replication factor C from its five subunits". Proceedings of the National Academy of Sciences of the United States of America. 93 (13): 6521–6. Bibcode:1996PNAS...93.6521U. doi: 10.1073/pnas.93.13.6521 . PMC   39056 . PMID   8692848.
• Mossi R, Jónsson ZO, Allen BL, Hardin SH, Hübscher U (Jan 1997). "Replication factor C interacts with the C-terminal side of proliferating cell nuclear antigen". The Journal of Biological Chemistry. 272 (3): 1769–76. doi: 10.1074/jbc.272.3.1769 . PMID   8999859.
• Cujec TP, Cho H, Maldonado E, Meyer J, Reinberg D, Peterlin BM (Apr 1997). "The human immunodeficiency virus transactivator Tat interacts with the RNA polymerase II holoenzyme". Molecular and Cellular Biology. 17 (4): 1817–23. doi:10.1128/mcb.17.4.1817. PMC   232028 . PMID   9121429.
• Cai J, Gibbs E, Uhlmann F, Phillips B, Yao N, O'Donnell M, Hurwitz J (Jul 1997). "A complex consisting of human replication factor C p40, p37, and p36 subunits is a DNA-dependent ATPase and an intermediate in the assembly of the holoenzyme". The Journal of Biological Chemistry. 272 (30): 18974–81. doi: 10.1074/jbc.272.30.18974 . PMID   9228079.
• Loor G, Zhang SJ, Zhang P, Toomey NL, Lee MY (Dec 1997). "Identification of DNA replication and cell cycle proteins that interact with PCNA". Nucleic Acids Research. 25 (24): 5041–6. doi:10.1093/nar/25.24.5041. PMC   147130 . PMID   9396813.
• Ellison V, Stillman B (Mar 1998). "Reconstitution of recombinant human replication factor C (RFC) and identification of an RFC subcomplex possessing DNA-dependent ATPase activity" (PDF). The Journal of Biological Chemistry. 273 (10): 5979–87. doi: 10.1074/jbc.273.10.5979 . PMID   9488738. S2CID   17647091.
• Cai J, Yao N, Gibbs E, Finkelstein J, Phillips B, O'Donnell M, Hurwitz J (Sep 1998). "ATP hydrolysis catalyzed by human replication factor C requires participation of multiple subunits". Proceedings of the National Academy of Sciences of the United States of America. 95 (20): 11607–12. Bibcode:1998PNAS...9511607C. doi: 10.1073/pnas.95.20.11607 . PMC   21688 . PMID   9751713.
• Zhang G, Gibbs E, Kelman Z, O'Donnell M, Hurwitz J (Mar 1999). "Studies on the interactions between human replication factor C and human proliferating cell nuclear antigen". Proceedings of the National Academy of Sciences of the United States of America. 96 (5): 1869–74. Bibcode:1999PNAS...96.1869Z. doi: 10.1073/pnas.96.5.1869 . PMC   26703 . PMID   10051561.
• Lindsey-Boltz LA, Bermudez VP, Hurwitz J, Sancar A (Sep 2001). "Purification and characterization of human DNA damage checkpoint Rad complexes". Proceedings of the National Academy of Sciences of the United States of America. 98 (20): 11236–41. Bibcode:2001PNAS...9811236L. doi: 10.1073/pnas.201373498 . PMC   58713 . PMID   11572977.
• Griffith JD, Lindsey-Boltz LA, Sancar A (May 2002). "Structures of the human Rad17-replication factor C and checkpoint Rad 9-1-1 complexes visualized by glycerol spray/low voltage microscopy". The Journal of Biological Chemistry. 277 (18): 15233–6. doi: 10.1074/jbc.C200129200 . PMID   11907025.
• Ohta S, Shiomi Y, Sugimoto K, Obuse C, Tsurimoto T (Oct 2002). "A proteomics approach to identify proliferating cell nuclear antigen (PCNA)-binding proteins in human cell lysates. Identification of the human CHL12/RFCs2-5 complex as a novel PCNA-binding protein". The Journal of Biological Chemistry. 277 (43): 40362–7. doi: 10.1074/jbc.M206194200 . PMID   12171929.
• Maruyama T, Farina A, Dey A, Cheong J, Bermudez VP, Tamura T, Sciortino S, Shuman J, Hurwitz J, Ozato K (Sep 2002). "A Mammalian bromodomain protein, brd4, interacts with replication factor C and inhibits progression to S phase". Molecular and Cellular Biology. 22 (18): 6509–20. doi:10.1128/MCB.22.18.6509-6520.2002. PMC   135621 . PMID   12192049.
• Venclovas C, Colvin ME, Thelen MP (Oct 2002). "Molecular modeling-based analysis of interactions in the RFC-dependent clamp-loading process". Protein Science. 11 (10): 2403–16. doi:10.1110/ps.0214302. PMC   2373712 . PMID   12237462.
• Merkle CJ, Karnitz LM, Henry-Sánchez JT, Chen J (Aug 2003). "Cloning and characterization of hCTF18, hCTF8, and hDCC1. Human homologs of a Saccharomyces cerevisiae complex involved in sister chromatid cohesion establishment". The Journal of Biological Chemistry. 278 (32): 30051–6. doi: 10.1074/jbc.M211591200 . PMID   12766176.
• Bermudez VP, Maniwa Y, Tappin I, Ozato K, Yokomori K, Hurwitz J (Sep 2003). "The alternative Ctf18-Dcc1-Ctf8-replication factor C complex required for sister chromatid cohesion loads proliferating cell nuclear antigen onto DNA". Proceedings of the National Academy of Sciences of the United States of America. 100 (18): 10237–42. Bibcode:2003PNAS..10010237B. doi: 10.1073/pnas.1434308100 . PMC   193545 . PMID   12930902.
• Zou L, Liu D, Elledge SJ (Nov 2003). "Replication protein A-mediated recruitment and activation of Rad17 complexes". Proceedings of the National Academy of Sciences of the United States of America. 100 (24): 13827–32. Bibcode:2003PNAS..10013827Z. doi: 10.1073/pnas.2336100100 . PMC   283506 . PMID   14605214.