SUPT5H

SUPT5H
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 2DO3, 2E6Z, 2E70, 3H7H, 4L1U
Identifiers
Aliases	SUPT5H , SPT5, SPT5H, Tat-CT1, SPT5 homolog, DSIF elongation factor subunit
External IDs	OMIM: 602102; MGI: 1202400; HomoloGene: 2384; GeneCards: SUPT5H; OMA:SUPT5H - orthologs
Gene location (Human) Chr. Chromosome 19 (human) [1] Band 19q13.2 Start 39,436,156 bp [1] End 39,476,670 bp [1]
Gene location (Mouse) Chr. Chromosome 7 (mouse) [2] Band 7 A3|7 16.68 cM Start 28,014,316 bp [2] End 28,038,171 bp [2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in right testis left testis anterior pituitary sural nerve right hemisphere of cerebellum right lobe of thyroid gland right uterine tube left lobe of thyroid gland apex of heart right frontal lobe Top expressed in adrenal gland Gonadal ridge internal carotid artery external carotid artery fossa crypt of lieberkuhn of small intestine lacrimal gland tail of embryo vestibular membrane of cochlear duct motor neuron More reference expression data BioGPS More reference expression data
Gene ontology Molecular function protein binding protein heterodimerization activity enzyme binding chromatin binding RNA binding DNA binding mRNA binding Cellular component nucleoplasm DSIF complex nucleus Biological process chromatin remodeling negative regulation of mRNA polyadenylation regulation of transcription, DNA-templated regulation of transcription by RNA polymerase II transcription elongation from RNA polymerase II promoter regulation of DNA-templated transcription, elongation negative regulation of transcription by RNA polymerase II 7-methylguanosine mRNA capping transcription by RNA polymerase II response to organic substance transcription, DNA-templated negative regulation of DNA-templated transcription, elongation positive regulation of DNA-templated transcription, elongation positive regulation of macroautophagy DNA-templated transcription, elongation single stranded viral RNA replication via double stranded DNA intermediate positive regulation of viral transcription cell cycle positive regulation of transcription by RNA polymerase II Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 6829 20924 Ensembl ENSG00000196235 ENSMUSG00000003435 UniProt O00267 O55201 RefSeq (mRNA) NM_001111020 NM_001130824 NM_001130825 NM_003169 NM_001319990 NM_001319991 NM_013676 RefSeq (protein) NP_001104490 NP_001124296 NP_001124297 NP_001306919 NP_001306920 NP_003160 NP_038704 Location (UCSC) Chr 19: 39.44 – 39.48 Mb Chr 7: 28.01 – 28.04 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

Transcription elongation factor SPT5 is a protein that in humans is encoded by the SUPT5H gene. ^{[5] [6]}

Interactions

SUPT5H has been shown to interact with:

• CDK9, ^[7]
• Cyclin-dependent kinase 7, ^[7]
• HTATSF1, ^[8]
• PIN1, ^[9]
• POLR2A, ^{[8] [10]}
• PRMT1 ^[11] and
• Protein arginine methyltransferase 5. ^[11]

References

1. GRCh38: Ensembl release 89: ENSG00000196235 – Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000003435 – Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Chiang PW, Fogel E, Jackson CL, Lieuallen K, Lennon G, Qu X, Wang SQ, Kurnit DM (Dec 1996). "Isolation, sequencing, and mapping of the human homologue of the yeast transcription factor, SPT5". Genomics. 38 (3): 421–4. doi:10.1006/geno.1996.0646. PMID   8975720.
6. "Entrez Gene: SUPT5H Suppressor of Ty 5 homolog (S. cerevisiae)".
7. Garber ME, Mayall TP, Suess EM, Meisenhelder J, Thompson NE, Jones KA (Sep 2000). "CDK9 autophosphorylation regulates high-affinity binding of the human immunodeficiency virus type 1 tat-P-TEFb complex to TAR RNA". Molecular and Cellular Biology. 20 (18): 6958–69. doi:10.1128/mcb.20.18.6958-6969.2000. PMC   88771 . PMID   10958691.
8. Kim JB, Yamaguchi Y, Wada T, Handa H, Sharp PA (Sep 1999). "Tat-SF1 protein associates with RAP30 and human SPT5 proteins". Molecular and Cellular Biology. 19 (9): 5960–8. doi:10.1128/mcb.19.9.5960. PMC   84462 . PMID   10454543.
9. Lavoie SB, Albert AL, Handa H, Vincent M, Bensaude O (Sep 2001). "The peptidyl-prolyl isomerase Pin1 interacts with hSpt5 phosphorylated by Cdk9". Journal of Molecular Biology. 312 (4): 675–85. doi:10.1006/jmbi.2001.4991. PMID   11575923.
10. Wada T, Takagi T, Yamaguchi Y, Ferdous A, Imai T, Hirose S, Sugimoto S, Yano K, Hartzog GA, Winston F, Buratowski S, Handa H (Feb 1998). "DSIF, a novel transcription elongation factor that regulates RNA polymerase II processivity, is composed of human Spt4 and Spt5 homologs". Genes & Development. 12 (3): 343–56. doi:10.1101/gad.12.3.343. PMC   316480 . PMID   9450929.
11. Kwak YT, Guo J, Prajapati S, Park KJ, Surabhi RM, Miller B, Gehrig P, Gaynor RB (Apr 2003). "Methylation of SPT5 regulates its interaction with RNA polymerase II and transcriptional elongation properties". Molecular Cell. 11 (4): 1055–66. doi: 10.1016/s1097-2765(03)00101-1 . PMID   12718890.

Further reading

• Andersson B, Wentland MA, Ricafrente JY, Liu W, Gibbs RA (Apr 1996). "A "double adaptor" method for improved shotgun library construction". Analytical Biochemistry. 236 (1): 107–13. doi:10.1006/abio.1996.0138. PMID   8619474.
• Yu W, Andersson B, Worley KC, Muzny DM, Ding Y, Liu W, Ricafrente JY, Wentland MA, Lennon G, Gibbs RA (Apr 1997). "Large-scale concatenation cDNA sequencing". Genome Research. 7 (4): 353–8. doi:10.1101/gr.7.4.353. PMC   139146 . PMID   9110174.
• Stachora AA, Schäfer RE, Pohlmeier M, Maier G, Ponstingl H (Jun 1997). "Human Supt5h protein, a putative modulator of chromatin structure, is reversibly phosphorylated in mitosis". FEBS Letters. 409 (1): 74–8. doi: 10.1016/S0014-5793(97)00486-9 . PMID   9199507. S2CID   43730950.
• Wada T, Takagi T, Yamaguchi Y, Ferdous A, Imai T, Hirose S, Sugimoto S, Yano K, Hartzog GA, Winston F, Buratowski S, Handa H (Feb 1998). "DSIF, a novel transcription elongation factor that regulates RNA polymerase II processivity, is composed of human Spt4 and Spt5 homologs". Genes & Development. 12 (3): 343–56. doi:10.1101/gad.12.3.343. PMC   316480 . PMID   9450929.
• Wu-Baer F, Lane WS, Gaynor RB (Mar 1998). "Role of the human homolog of the yeast transcription factor SPT5 in HIV-1 Tat-activation". Journal of Molecular Biology. 277 (2): 179–97. doi:10.1006/jmbi.1997.1601. PMID   9514752.
• Wada T, Takagi T, Yamaguchi Y, Watanabe D, Handa H (Dec 1998). "Evidence that P-TEFb alleviates the negative effect of DSIF on RNA polymerase II-dependent transcription in vitro". The EMBO Journal. 17 (24): 7395–403. doi:10.1093/emboj/17.24.7395. PMC   1171084 . PMID   9857195.
• Yamaguchi Y, Wada T, Watanabe D, Takagi T, Hasegawa J, Handa H (Mar 1999). "Structure and function of the human transcription elongation factor DSIF". The Journal of Biological Chemistry. 274 (12): 8085–92. doi: 10.1074/jbc.274.12.8085 . PMID   10075709.
• Yamaguchi Y, Takagi T, Wada T, Yano K, Furuya A, Sugimoto S, Hasegawa J, Handa H (Apr 1999). "NELF, a multisubunit complex containing RD, cooperates with DSIF to repress RNA polymerase II elongation". Cell. 97 (1): 41–51. doi: 10.1016/S0092-8674(00)80713-8 . PMID   10199401. S2CID   16855257.
• Parada CA, Roeder RG (Jul 1999). "A novel RNA polymerase II-containing complex potentiates Tat-enhanced HIV-1 transcription". The EMBO Journal. 18 (13): 3688–701. doi:10.1093/emboj/18.13.3688. PMC   1171446 . PMID   10393184.
• Wen Y, Shatkin AJ (Jul 1999). "Transcription elongation factor hSPT5 stimulates mRNA capping". Genes & Development. 13 (14): 1774–9. doi:10.1101/gad.13.14.1774. PMC   316881 . PMID   10421630.
• Kim JB, Yamaguchi Y, Wada T, Handa H, Sharp PA (Sep 1999). "Tat-SF1 protein associates with RAP30 and human SPT5 proteins". Molecular and Cellular Biology. 19 (9): 5960–8. doi:10.1128/mcb.19.9.5960. PMC   84462 . PMID   10454543.
• Ivanov D, Kwak YT, Guo J, Gaynor RB (May 2000). "Domains in the SPT5 protein that modulate its transcriptional regulatory properties". Molecular and Cellular Biology. 20 (9): 2970–83. doi:10.1128/MCB.20.9.2970-2983.2000. PMC   85557 . PMID   10757782.
• Wada T, Orphanides G, Hasegawa J, Kim DK, Shima D, Yamaguchi Y, Fukuda A, Hisatake K, Oh S, Reinberg D, Handa H (Jun 2000). "FACT relieves DSIF/NELF-mediated inhibition of transcriptional elongation and reveals functional differences between P-TEFb and TFIIH". Molecular Cell. 5 (6): 1067–72. doi: 10.1016/S1097-2765(00)80272-5 . PMID   10912001.
• Ping YH, Rana TM (Apr 2001). "DSIF and NELF interact with RNA polymerase II elongation complex and HIV-1 Tat stimulates P-TEFb-mediated phosphorylation of RNA polymerase II and DSIF during transcription elongation". The Journal of Biological Chemistry. 276 (16): 12951–8. doi: 10.1074/jbc.M006130200 . PMID   11112772.
• Kim JB, Sharp PA (Apr 2001). "Positive transcription elongation factor B phosphorylates hSPT5 and RNA polymerase II carboxyl-terminal domain independently of cyclin-dependent kinase-activating kinase". The Journal of Biological Chemistry. 276 (15): 12317–23. doi: 10.1074/jbc.M010908200 . PMID   11145967.
• Renner DB, Yamaguchi Y, Wada T, Handa H, Price DH (Nov 2001). "A highly purified RNA polymerase II elongation control system". The Journal of Biological Chemistry. 276 (45): 42601–9. doi: 10.1074/jbc.M104967200 . PMID   11553615.
• Lavoie SB, Albert AL, Handa H, Vincent M, Bensaude O (Sep 2001). "The peptidyl-prolyl isomerase Pin1 interacts with hSpt5 phosphorylated by Cdk9". Journal of Molecular Biology. 312 (4): 675–85. doi:10.1006/jmbi.2001.4991. PMID   11575923.
• Bourgeois CF, Kim YK, Churcher MJ, West MJ, Karn J (Feb 2002). "Spt5 cooperates with human immunodeficiency virus type 1 Tat by preventing premature RNA release at terminator sequences". Molecular and Cellular Biology. 22 (4): 1079–93. doi:10.1128/MCB.22.4.1079-1093.2002. PMC   134635 . PMID   11809800.
• Yamaguchi Y, Inukai N, Narita T, Wada T, Handa H (May 2002). "Evidence that negative elongation factor represses transcription elongation through binding to a DRB sensitivity-inducing factor/RNA polymerase II complex and RNA". Molecular and Cellular Biology. 22 (9): 2918–27. doi:10.1128/MCB.22.9.2918-2927.2002. PMC   133766 . PMID   11940650.