Uganda sent a delegation to compete at the 2016 Summer Paralympics in Rio de Janeiro, Brazil, from 7 to 18 September 2016. This was the eighth appearance of the country in the Summer Paralympic Games after it debuted forty-four years prior at the 1972 Heidelberg Paralympics. Athletics track runner David Emong was the sole athlete representing Uganda in Rio de Janeiro. He took part in the men's 400 metres T45–47 competition on 8 September and did not qualify for the finals because he was fifteenth overall. Emong participated in the men's 1500 metres T45–46 event later that day and he took Uganda's first medal in Paralympic competition by coming second in the final.
Senegal sent a delegation to compete at the 2016 Summer Paralympics in Rio de Janeiro, Brazil, from 7 to 18 September 2016. This was the fourth successive appearance for the nation in a Summer Paralympic Games after it debuted at the 2004 Summer Paralympics. Youssouphua Diouf, a javelin thrower, and shot put and discus thrower Daque Diop were the two athletes sent to Rio de Janeiro by Senegal. The delegation failed to win the country's first medal at the Summer Paralympics as its best performance in these Games was Diouf's seventh position in the men's javelin F56-57 event.
Mozambique sent a delegation to compete at the 2016 Summer Paralympics in Rio de Janeiro, Brazil, from 7 to 18 September 2016. This was the country's second time competing at a Summer Paralympic Games after making its debut at the 2012 Summer Paralympics. Mozambique was represented by one athlete, Edmilisa Governo, a short-distance sprinter. She competed in two events, the women's 100 metres T12 competition and the women's 400 metres T12. Governo reached the semi-finals of the women's 100 metres T12 and took Mozambique's first Paralympic Games medal in the women's 400 metres T12 by placing third in the final of the competition.
Mauritius sent a delegation to compete at the 2016 Summer Paralympics in Rio de Janeiro, Brazil, from 7–18 September 2016. This was the fifth time the country had taken part in a Paralympic Games after its debut at the 1996 Summer Paralympics. The Mauritian delegation to Rio de Janeiro consisted of two athletes: wheelchair racer Brandy Perrine and short-distance swimmer Scody Victor. The nation's best result was tenth overall by Perrine in the women's 100 metres T54 event as both competitors did not progress to the final in their respective competitions.
Lesotho sent a delegation to compete at the 2016 Summer Paralympics in Rio de Janeiro, Brazil, from 7 to 18 September 2016. This was the fifth time the country competed in the Summer Paralympic Games after it made its debut sixteen years prior at the 2000 Sydney Paralympics. The delegation to Rio de Janeiro consisted of two athletes: sprinter Sello Mothebe and discus thrower Litsitso Khotele. Mothebe originally came third in the heats of the men's 200 metres T12 and the men's 400 metres T12 events but he was retroactively disqualified for testing positive for a banned substance. Khotele ranked tenth in the women's discus throw F43–44 competition with a throw of 19.91 metres.
Gabon sent a delegation to compete at the 2016 Summer Paralympics in Rio de Janeiro, Brazil, from 7–18 September 2016. This was the nation's third appearance at a Summer Paralympic Games, following their two previous participations at the 2008 Summer Paralympics and the 2012 Summer Paralympics. Gabon sent a sole athlete to represent them at Rio de Janeiro, wheelchair racer Edmond Ngombi. He did not advance from his heat in the men's 100 metres T54 event as he came sixth out of seven competitors and attributed the result to a handlebar problem.
Cape Verde sent a delegation to compete at the 2016 Summer Paralympics in Rio de Janeiro, Brazil, from 7 to 18 September 2016. This was the nations' fourth appearance at a Summer Paralympic Games since it made its debut at the 2004 Athens Summer Paralympics. Cape Verde was represented by two athletes in Rio de Janeiro: sprinter Gracelino Barbosa and javelin thrower Márcio Fernandes, who qualified for the Games by meeting the qualification standards of their events. Barbosa won the country's first Paralympic medal with his third-place finish in the men's 400 metres T20 competition and Fernandes came ninth in the F44 men's javelin event.
Botswana sent a delegation to compete at the 2016 Summer Paralympics in Rio de Janeiro, Brazil, from 7 to 18 September 2016. This was the country's second time competing at a Summer Paralympic Games after making its debut at the 2004 Summer Paralympics. Botswana was represented by one athlete, Keatlaretese Mabote, a short-distance sprinter. He competed in one event, the men's 400 metres T12 competition, where he was eliminated in the heat stages because he was third in his heat and only the fop two participants in a heat progressed to the semi-finals.
Panama sent a delegation to compete at the 2016 Summer Paralympics in Rio de Janeiro, Brazil, from 7–18 September 2016. This was the nation's seventh time competing in the Summer Paralympic Games after it first entered the movement at the 1992 Summer Paralympics. The Panamanian delegation to Rio de Janeiro consisted of two athletes: shot put thrower Francisco Cedeño Almengor and javelin thrower Iveth Valdes Romero. Almengor ranked eleventh out of twelve athletes in the men's shot put F54–55 competition and Romero placed in the same position in the women's javelin F55–F56 event.
Nicaragua sent a delegation to compete at the 2016 Summer Paralympics in Rio de Janeiro, Brazil, from 7–18 September 2016. This was the Central American country's third appearance at the Summer Paralympic Games, having made its debut twelve years earlier at the 2004 Summer Paralympics. They were represented by three athletes, sprinter Jennifer Osejo, middle-distance runner and sprinter Gabriel Cuadra Holmann and powerlifter Fernando Acevedo, who all qualified for the games by achieving the minimum qualifying standard in international competition. Neither Holmann or Osejo claimed a medal in their respective events and Acevado finished fifth in the men's −72kg powerlifting class.
Honduras sent a delegation to compete at the 2016 Summer Paralympics in Rio de Janeiro, Brazil, from 7–18 September 2016. This was the nation's sixth appearance at a Summer Paralympiad after it debuted at the 1996 Summer Paralympics. The Honduran delegation to Rio de Janeiro consisted of two athletes: powerlifter Gabriel Zelaya Díaz and short-distance swimmer Emmanuel Díaz. Both competitors were not ranked in their respective competitions after Gabriel Zelaya Díaz was unable to lift any weights in his three tries and Emmanuel Díaz was two minutes late arriving to his event.
Guatemala sent a delegation to compete at the 2016 Summer Paralympics in Rio de Janeiro, Brazil, from 7–18 September 2016. This was the nation's seventh time competing in the Summer Paralympic Games since it made its debut forty years prior in Toronto, Canada. Middle-distance runner Óscar Raxón Siquiej was the only athlete that Guatemala sent to Rio de Janeiro after he was awarded a wild card spot by the International Paralympic Committee. He was third and last in his heat in the men's 1500 metres T11 and failed to advance to the final since only the top six were allowed in that stage of the competition.
Aruba competed in the 2016 Summer Paralympics in Rio de Janeiro, Brazil from 7 to 18 September 2016. The country's participation in Rio marked its debut appearance in the quadrennial event, although it had competed in the Summer Olympics eight times since the 1988 Games. The delegation consisted of a single short-distance swimmer, Jesus De Marchena Acevedo, who qualified for the Games by using a wildcard. He was chosen as the flag bearer for the opening ceremony and was disqualified from the first discipline he took part in, men's 50 metres freestyle (S7), for arriving late but later placed last overall in the 100 metres freestyle (S7).
Afghanistan sent a delegation to compete at the 2016 Summer Paralympics in Rio de Janeiro, Brazil, from 7–18 September 2016. This was the nation's fifth time taking part in a Summer Paralympic Games. The Afghan delegation consisted of a single athlete, Mohammad Durani, who competed in the javelin throw. Originally he finished 16th in his event, but he was retroactively disqualified for a doping violation.
Macau sent a delegation to compete at the 2016 Summer Paralympics in Rio de Janeiro, Brazil, from 7–18 September 2016. This was the territory's eighth time competing at a Summer Paralympic Games since it debuted at the 1988 Summer Paralympics. The Macanese delegation to Rio consisted of one athlete, 18-year old swimmer Chen Yu Chia, who qualified for the Games by being granted a wild card place from the International Paralympic Committee in August 2016. His best finish was 17th in the men's 200 metre freestyle S14 tournament, and his other result was 19th in the men's 200 metre individual medley SM14.
Tajikistan sent a delegation to compete at the 2016 Summer Paralympics in Rio de Janeiro, Brazil, from 7–18 September 2016. This was the fourth consecutive appearance of the country at the Paralympic Games after it made its debut twelve years prior at the 2004 Summer Paralympics. Tajikstan was represented by a single athlete in Rio de Janeiro: sprinter Romikhudo Dodikhudoev. He finished 14th overall in both of the men's 400 metres T47 and the men's 100 metres T47 and these performances meant he did not qualify for the final of both competitions.
Palestine sent a delegation to compete at the 2016 Summer Paralympics in Rio de Janeiro, Brazil, from 7–18 September 2016. This was the fifth time they had taken part in a Paralympic Games after its debut sixteen years prior at the 2000 Summer Paralympics. Palestine sent one athlete to Rio de Janeiro shot put thrower Husam Azzam, who was the flag bearer for Palestine at the parade of nations during the opening ceremony. He did not win his third Paralympic medal because he ranked eighth out of ten athletes with a throw of 6.34 metres in the men's shot put F53 competition.
Nepal sent a delegation to compete at the 2016 Summer Paralympics in Rio de Janeiro, Brazil, from 7–18 September 2016. This was the fourth time the nation had taken part in a Paralympic Games following its first appearance at the 2004 Summer Paralympics. Nepal was represented by two athletes in Rio de Janeiro: sprinter Bikram Rana and short-distance swimmer Laxmi Kunwar, who both qualified for the Paralympics by using wild card spots for their respective sports. Neither athlete advanced beyond the first round of their respective events as they both finished 17th overall in their competitions.
Montenegro sent a delegation to compete at the 2016 Summer Paralympics in Rio de Janeiro, Brazil, from 7 to 18 September 2016. This was the nation's third time competing at a Paralympic Games since it became independent from its federation with Serbia in June 2006. Montenegro was represented by two athletes in Rio de Janeiro: shot putter and discus thrower Marijana Goranović and short-distance swimmer Ilija Tadić. Goranović competed in two events in field and her best finish was in the women's shot put F41 competition, where she came eighth. Tadić took part in two swimming events and his best performance was in the men's 50 metre freestyle S9 tournament, where he placed 14th overall and did not advance to the final.
Cyprus sent a delegation to compete at the 2016 Summer Paralympics in Rio de Janeiro, Brazil, from 7 to 18 September 2016. This was the island country's eighth consecutive appearance in a Summer Paralympiad having made its debut at the 1988 Summer Paralympics. The Cypriot delegation to Rio de Janeiro consisted of two athletes: sprinter Antonis Aresti and short-distance swimmer Karolina Pelendritou. Aresti placed sixth overall in the men's 400 metres T47 event and Pelendritou came fourth in the 100 metres breaststroke SB13 competition after losing the bronze medal by 20 cm (7.9 in) in the final.
