TAF15

TAF15
Available structures PDB Human UniProt search: PDBe RCSB List of PDB id codes 2MMY
Identifiers
Aliases	TAF15 , Npl3, RBP56, TAF2N, TAFII68, TATA-box binding protein associated factor 15
External IDs	OMIM: 601574; MGI: 1917689; HomoloGene: 131088; GeneCards: TAF15; OMA:TAF15 - orthologs
Gene location (Human) Chr. Chromosome 17 (human) [1] Band 17q12 Start 35,809,482 bp [1] End 35,864,615 bp [1]
Gene location (Mouse) Chr. Chromosome 11 (mouse) [2] Band 11|11 C Start 83,473,086 bp [2] End 83,506,743 bp [2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in endometrium ganglionic eminence right testis left testis monocyte lymph node zone of skin skin of abdomen skin of leg gastric mucosa Top expressed in neural layer of retina tail of embryo ventricular zone yolk sac genital tubercle aortic valve dentate gyrus of hippocampal formation granule cell ascending aorta esophagus granulocyte More reference expression data BioGPS More reference expression data
Gene ontology Molecular function DNA binding protein binding metal ion binding nucleic acid binding RNA binding Cellular component cytoplasm nucleolus nucleus nucleoplasm Biological process positive regulation of transcription, DNA-templated regulation of transcription, DNA-templated transcription by RNA polymerase II transcription initiation from RNA polymerase II promoter regulation of signal transduction by p53 class mediator Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 8148 70439 Ensembl ENSG00000270647 ENSG00000276833 ENSMUSG00000020680 UniProt Q92804 n/a RefSeq (mRNA) NM_003487 NM_139215 NM_027427 RefSeq (protein) NP_003478 NP_631961 n/a Location (UCSC) Chr 17: 35.81 – 35.86 Mb Chr 11: 83.47 – 83.51 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

TATA-binding protein-associated factor 2N is a protein that in humans is encoded by the TAF15 gene. ^{[5] [6] [7]}

Function

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes a subunit of TFIID present in a subset of TFIID complexes. Translocations involving chromosome 17 and chromosome 9, where the gene for the nuclear receptor CSMF is located, result in a gene fusion product that is an RNA binding protein associated with a subset of extraskeletal myxoid chondrosarcomas. Two transcripts encoding different isoforms have been identified. ^[7]

Interactions

TAF15 has been shown to interact with:

• POLR2C, ^[8]
• POLR2E, ^[8]
• POLR2G, ^[8]
• SAFB, ^[9]
• TAF11, ^[8]
• TAF13, ^[8]
• TAF5, ^[8]
• TAF7, ^[8] and
• TATA binding protein ^[10]

References

1. ENSG00000276833 GRCh38: Ensembl release 89: ENSG00000270647, ENSG00000276833 – Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000020680 – Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Morohoshi F, Arai K, Takahashi EI, Tanigami A, Ohki M (November 15, 1996). "Cloning and mapping of a human RBP56 gene encoding a putative RNA binding protein similar to FUS/TLS and EWS proteins". Genomics. 38 (1): 51–7. doi:10.1006/geno.1996.0591. PMID   8954779.
6. Morohoshi F, Ootsuka Y, Arai K, Ichikawa H, Mitani S, Munakata N, Ohki M (Dec 1998). "Genomic structure of the human RBP56/hTAFII68 and FUS/TLS genes". Gene. 221 (2): 191–8. doi:10.1016/S0378-1119(98)00463-6. PMID   9795213.
7. "Entrez Gene: TAF15 TAF15 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 68kDa".
8. Bertolotti A, Melot T, Acker J, Vigneron M, Delattre O, Tora L (Mar 1998). "EWS, but not EWS-FLI-1, is associated with both TFIID and RNA polymerase II: interactions between two members of the TET family, EWS and hTAFII68, and subunits of TFIID and RNA polymerase II complexes". Mol. Cell. Biol. 18 (3): 1489–97. doi:10.1128/mcb.18.3.1489. PMC   108863 . PMID   9488465.
9. Townson SM, Kang K, Lee AV, Oesterreich S (Jun 2004). "Structure-function analysis of the estrogen receptor alpha corepressor scaffold attachment factor-B1: identification of a potent transcriptional repression domain". J. Biol. Chem. 279 (25): 26074–81. doi: 10.1074/jbc.M313726200 . PMID   15066997.
10. Hoffmann A, Roeder RG (Jul 1996). "Cloning and characterization of human TAF20/15. Multiple interactions suggest a central role in TFIID complex formation". J. Biol. Chem. 271 (30): 18194–202. doi: 10.1074/jbc.271.30.18194 . PMID   8663456.

Further reading

• Zhou Q, Sharp PA (1995). "Novel mechanism and factor for regulation by HIV-1 Tat". EMBO J. 14 (2): 321–8. doi:10.1002/j.1460-2075.1995.tb07006.x. PMC   398086 . PMID   7835343.
• Parada CA, Yoon JB, Roeder RG (1995). "A novel LBP-1-mediated restriction of HIV-1 transcription at the level of elongation in vitro". J. Biol. Chem. 270 (5): 2274–83. doi: 10.1074/jbc.270.5.2274 . PMID   7836461.
• Ou SH, Garcia-Martínez LF, Paulssen EJ, Gaynor RB (1994). "Role of flanking E box motifs in human immunodeficiency virus type 1 TATA element function". J. Virol. 68 (11): 7188–99. doi:10.1128/JVI.68.11.7188-7199.1994. PMC   237158 . PMID   7933101.
• Kashanchi F, Piras G, Radonovich MF, Duvall JF, Fattaey A, Chiang CM, Roeder RG, Brady JN (1994). "Direct interaction of human TFIID with the HIV-1 transactivator tat". Nature. 367 (6460): 295–9. Bibcode:1994Natur.367..295K. doi:10.1038/367295a0. PMID   8121496. S2CID   4362048.
• Hoffmann A, Roeder RG (1996). "Cloning and characterization of human TAF20/15. Multiple interactions suggest a central role in TFIID complex formation". J. Biol. Chem. 271 (30): 18194–202. doi: 10.1074/jbc.271.30.18194 . PMID   8663456.
• Wang Z, Morris GF, Rice AP, Xiong W, Morris CB (1996). "Wild-type and transactivation-defective mutants of human immunodeficiency virus type 1 Tat protein bind human TATA-binding protein in vitro". J. Acquir. Immune Defic. Syndr. Hum. Retrovirol. 12 (2): 128–38. doi: 10.1097/00042560-199606010-00005 . PMID   8680883.
• Pendergrast PS, Morrison D, Tansey WP, Hernandez N (1996). "Mutations in the carboxy-terminal domain of TBP affect the synthesis of human immunodeficiency virus type 1 full-length and short transcripts similarly". J. Virol. 70 (8): 5025–34. doi:10.1128/JVI.70.8.5025-5034.1996. PMC   190456 . PMID   8764009.
• Kashanchi F, Khleif SN, Duvall JF, Sadaie MR, Radonovich MF, Cho M, Martin MA, Chen SY, Weinmann R, Brady JN (1996). "Interaction of human immunodeficiency virus type 1 Tat with a unique site of TFIID inhibits negative cofactor Dr1 and stabilizes the TFIID-TFIIA complex". J. Virol. 70 (8): 5503–10. doi:10.1128/JVI.70.8.5503-5510.1996. PMC   190508 . PMID   8764062.
• Zhou Q, Sharp PA (1996). "Tat-SF1: cofactor for stimulation of transcriptional elongation by HIV-1 Tat". Science. 274 (5287): 605–10. Bibcode:1996Sci...274..605Z. doi:10.1126/science.274.5287.605. PMID   8849451. S2CID   13266489.
• Bertolotti A, Lutz Y, Heard DJ, Chambon P, Tora L (1996). "hTAF(II)68, a novel RNA/ssDNA-binding protein with homology to the pro-oncoproteins TLS/FUS and EWS is associated with both TFIID and RNA polymerase II". EMBO J. 15 (18): 5022–31. doi:10.1002/j.1460-2075.1996.tb00882.x. PMC   452240 . PMID   8890175.
• García-Martínez LF, Ivanov D, Gaynor RB (1997). "Association of Tat with purified HIV-1 and HIV-2 transcription preinitiation complexes". J. Biol. Chem. 272 (11): 6951–8. doi: 10.1074/jbc.272.11.6951 . PMID   9054383.
• Bertolotti A, Melot T, Acker J, Vigneron M, Delattre O, Tora L (1998). "EWS, but not EWS-FLI-1, is associated with both TFIID and RNA polymerase II: interactions between two members of the TET family, EWS and hTAFII68, and subunits of TFIID and RNA polymerase II complexes". Mol. Cell. Biol. 18 (3): 1489–97. doi:10.1128/mcb.18.3.1489. PMC   108863 . PMID   9488465.
• Zhang D, Paley AJ, Childs G (1998). "The transcriptional repressor ZFM1 interacts with and modulates the ability of EWS to activate transcription". J. Biol. Chem. 273 (29): 18086–91. doi: 10.1074/jbc.273.29.18086 . PMID   9660765.
• Martini A, La Starza R, Janssen H, Bilhou-Nabera C, Corveleyn A, Somers R, Aventin A, Foà R, Hagemeijer A, Mecucci C, Marynen P (2002). "Recurrent rearrangement of the Ewing's sarcoma gene, EWSR1, or its homologue, TAF15, with the transcription factor CIZ/NMP4 in acute leukemia". Cancer Res. 62 (19): 5408–12. PMID   12359745.
• Townson SM, Kang K, Lee AV, Oesterreich S (2004). "Structure-function analysis of the estrogen receptor alpha corepressor scaffold attachment factor-B1: identification of a potent transcriptional repression domain". J. Biol. Chem. 279 (25): 26074–81. doi: 10.1074/jbc.M313726200 . PMID   15066997.
• Lee HJ, Kim S, Pelletier J, Kim J (2004). "Stimulation of hTAFII68 (NTD)-mediated transactivation by v-Src". FEBS Lett. 564 (1–2): 188–98. doi: 10.1016/S0014-5793(04)00314-X . PMID   15094065. S2CID   1526475.
• Jin J, Smith FD, Stark C, Wells CD, Fawcett JP, Kulkarni S, Metalnikov P, O'Donnell P, Taylor P, Taylor L, Zougman A, Woodgett JR, Langeberg LK, Scott JD, Pawson T (2004). "Proteomic, functional, and domain-based analysis of in vivo 14-3-3 binding proteins involved in cytoskeletal regulation and cellular organization". Curr. Biol. 14 (16): 1436–50. doi: 10.1016/j.cub.2004.07.051 . PMID   15324660. S2CID   2371325.

External links

• Overview of all the structural information available in the PDB for UniProt : Q92804 (TATA-binding protein-associated factor 2N) at the PDBe-KB .