POLR2E

POLR2E
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 5IY6, 5IY9, 5IY8, 5IYA, 5IYB, 5IY7, 5IYC, 5IYD
Identifiers
Aliases	POLR2E , RPABC1, RPB5, XAP4, hRPB25, hsRPB5, polymerase (RNA) II subunit E, RNA polymerase II subunit E, RNA polymerase II, I and III subunit E
External IDs	OMIM: 180664; MGI: 1913670; HomoloGene: 2018; GeneCards: POLR2E; OMA:POLR2E - orthologs
Gene location (Human) Chr. Chromosome 19 (human) [1] Band 19p13.3 Start 1,086,574 bp [1] End 1,095,380 bp [1]
Gene location (Mouse) Chr. Chromosome 10 (mouse) [2] Band 10|10 C1 Start 79,871,783 bp [2] End 79,875,629 bp [2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in stromal cell of endometrium right lobe of liver prefrontal cortex right frontal lobe muscle of thigh muscle layer of sigmoid colon right adrenal gland popliteal artery abdominal fat tibial arteries Top expressed in yolk sac embryo morula embryo somite blastocyst epiblast mandibular prominence maxillary prominence otic placode More reference expression data BioGPS More reference expression data
Gene ontology Molecular function DNA binding RNA polymerase II activity RNA polymerase III activity protein binding RNA polymerase I activity DNA-directed 5'-3' RNA polymerase activity Cellular component cytosol RNA polymerase I complex RNA polymerase III complex RNA polymerase II, core complex nucleus nucleoplasm Biological process termination of RNA polymerase I transcription mRNA splicing, via spliceosome epigenetic maintenance of chromatin in transcription-competent conformation transcription initiation from RNA polymerase I promoter transcription elongation from RNA polymerase II promoter 7-methylguanosine mRNA capping transcription by RNA polymerase II transcription-coupled nucleotide-excision repair transcription initiation from RNA polymerase II promoter viral process transcription by RNA polymerase III fibroblast growth factor receptor signaling pathway snRNA transcription by RNA polymerase II RNA metabolic process regulation of gene silencing by miRNA transcription, DNA-templated transcription elongation from RNA polymerase I promoter positive regulation of type I interferon production somatic stem cell population maintenance positive regulation of viral transcription Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 5434 66420 Ensembl ENSG00000099817 ENSMUSG00000004667 UniProt P19388 Q80UW8 RefSeq (mRNA) NM_002695 NM_001316323 NM_001316324 NM_025554 RefSeq (protein) NP_001303252 NP_001303253 NP_002686 NP_079830 Location (UCSC) Chr 19: 1.09 – 1.1 Mb Chr 10: 79.87 – 79.88 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

DNA-directed RNA polymerases I, II, and III subunit RPABC1 is a protein that in humans is encoded by the POLR2E gene. ^[5]

This gene encodes the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. An interaction between this subunit and a hepatitis virus transactivating protein has been demonstrated, suggesting that interaction between transcriptional activators and the polymerase can occur through this subunit. A pseudogene is located on chromosome 11. ^[6]

Interactions

POLR2E has been shown to interact with TAF15, ^[7] POLR2C, ^[8] POLR2G, ^[8] POLR2H, ^[8] POLR2A, ^[8] POLR2B, ^[8] POLR2L ^[8] and GTF2F2. ^[9]

References

1. GRCh38: Ensembl release 89: ENSG00000099817 – Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000004667 – Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Acker J, Mattei MG, Wintzerith M, Roeckel N, Depetris D, Vigneron M, Kedinger C (Aug 1994). "Chromosomal localization of human RNA polymerase II subunit genes". Genomics. 20 (3): 496–499. doi:10.1006/geno.1994.1208. PMID   8034326.
6. "Entrez Gene: POLR2E polymerase (RNA) II (DNA directed) polypeptide E, 25kDa".
7. Bertolotti A, Melot T, Acker J, Vigneron M, Delattre O, Tora L (Mar 1998). "EWS, but not EWS-FLI-1, is associated with both TFIID and RNA polymerase II: interactions between two members of the TET family, EWS and hTAFII68, and subunits of TFIID and RNA polymerase II complexes". Mol. Cell. Biol. 18 (3). UNITED STATES: 1489–1497. doi:10.1128/mcb.18.3.1489. ISSN   0270-7306. PMC   108863 . PMID   9488465.
8. Acker J, de Graaff M, Cheynel I, Khazak V, Kedinger C, Vigneron M (Jul 1997). "Interactions between the human RNA polymerase II subunits". J. Biol. Chem. 272 (27). UNITED STATES: 16815–16821. doi: 10.1074/jbc.272.27.16815 . ISSN   0021-9258. PMID   9201987.
9. Wei W, Dorjsuren D, Lin Y, Qin W, Nomura T, Hayashi N, Murakami S (Apr 2001). "Direct interaction between the subunit RAP30 of transcription factor IIF (TFIIF) and RNA polymerase subunit 5, which contributes to the association between TFIIF and RNA polymerase II". J. Biol. Chem. 276 (15). United States: 12266–12273. doi: 10.1074/jbc.M009634200 . ISSN   0021-9258. PMID   11278533.

Further reading

• Jeang KT (1998). "Tat, Tat-associated kinase, and transcription". J. Biomed. Sci. 5 (1): 24–27. doi:10.1007/BF02253352. PMID   9570510.
• Yankulov K, Bentley D (1998). "Transcriptional control: Tat cofactors and transcriptional elongation". Curr. Biol. 8 (13): R447–9. Bibcode:1998CBio....8.R447Y. doi: 10.1016/S0960-9822(98)70289-1 . PMID   9651670. S2CID   15480646.
• Romano G, Kasten M, De Falco G, et al. (2000). "Regulatory functions of Cdk9 and of cyclin T1 in HIV tat transactivation pathway gene expression". J. Cell. Biochem. 75 (3): 357–368. doi:10.1002/(SICI)1097-4644(19991201)75:3<357::AID-JCB1>3.0.CO;2-K. PMID   10536359. S2CID   43685090.
• Marcello A, Zoppé M, Giacca M (2002). "Multiple modes of transcriptional regulation by the HIV-1 Tat transactivator". IUBMB Life. 51 (3): 175–181. doi: 10.1080/152165401753544241 . PMID   11547919. S2CID   10931640.
• Stevens M, De Clercq E, Balzarini J (2007). "The regulation of HIV-1 transcription: molecular targets for chemotherapeutic intervention". Med Res Rev. 26 (5): 595–625. doi:10.1002/med.20081. PMC   7168390 . PMID   16838299.
• Harrich D, McMillan N, Munoz L, et al. (2007). "Will diverse Tat interactions lead to novel antiretroviral drug targets?". Current Drug Targets. 7 (12): 1595–1606. doi:10.2174/138945006779025338. PMID   17168834.
• Kato H, Sumimoto H, Pognonec P, et al. (1992). "HIV-1 Tat acts as a processivity factor in vitro in conjunction with cellular elongation factors". Genes Dev. 6 (4): 655–666. doi: 10.1101/gad.6.4.655 . PMID   1559613.
• Pati UK, Weissman SM (1991). "Isolation and molecular characterization of a cDNA encoding the 23-kDa subunit of human RNA polymerase II". J. Biol. Chem. 266 (20): 13468. doi: 10.1016/S0021-9258(18)98863-0 . PMID   2071613.
• Southgate C, Zapp ML, Green MR (1990). "Activation of transcription by HIV-1 Tat protein tethered to nascent RNA through another protein". Nature. 345 (6276): 640–642. Bibcode:1990Natur.345..640S. doi:10.1038/345640a0. PMID   2190099. S2CID   4233742.
• Pati UK, Weissman SM (1989). "Isolation and molecular characterization of a cDNA encoding the 23-kDa subunit of human RNA polymerase II". J. Biol. Chem. 264 (22): 13114–13121. doi: 10.1016/S0021-9258(18)51603-3 . PMID   2753903.
• Wu-Baer F, Sigman D, Gaynor RB (1995). "Specific binding of RNA polymerase II to the human immunodeficiency virus trans-activating region RNA is regulated by cellular cofactors and Tat". Proc. Natl. Acad. Sci. U.S.A. 92 (16): 7153–7157. Bibcode:1995PNAS...92.7153W. doi: 10.1073/pnas.92.16.7153 . PMC   41297 . PMID   7638159.
• Cheong JH, Yi M, Lin Y, Murakami S (1995). "Human RPB5, a subunit shared by eukaryotic nuclear RNA polymerases, binds human hepatitis B virus X protein and may play a role in X transactivation". EMBO J. 14 (1): 143–150. doi:10.1002/j.1460-2075.1995.tb06984.x. PMC   398061 . PMID   7828586.
• Herrmann CH, Rice AP (1995). "Lentivirus Tat proteins specifically associate with a cellular protein kinase, TAK, that hyperphosphorylates the carboxyl-terminal domain of the large subunit of RNA polymerase II: candidate for a Tat cofactor". J. Virol. 69 (3): 1612–1620. doi:10.1128/jvi.69.3.1612-1620.1995. PMC   188757 . PMID   7853496.
• Keen NJ, Gait MJ, Karn J (1996). "Human immunodeficiency virus type-1 Tat is an integral component of the activated transcription-elongation complex". Proc. Natl. Acad. Sci. U.S.A. 93 (6): 2505–2510. Bibcode:1996PNAS...93.2505K. doi: 10.1073/pnas.93.6.2505 . PMC   39827 . PMID   8637904.
• Yang X, Herrmann CH, Rice AP (1996). "The human immunodeficiency virus Tat proteins specifically associate with TAK in vivo and require the carboxyl-terminal domain of RNA polymerase II for function". J. Virol. 70 (7): 4576–4584. doi:10.1128/jvi.70.7.4576-4584.1996. PMC   190394 . PMID   8676484.
• Agostini I, Navarro JM, Rey F, et al. (1996). "The human immunodeficiency virus type 1 Vpr transactivator: cooperation with promoter-bound activator domains and binding to TFIIB". J. Mol. Biol. 261 (5): 599–606. doi:10.1006/jmbi.1996.0485. PMID   8800208.
• Zhou Q, Sharp PA (1996). "Tat-SF1: cofactor for stimulation of transcriptional elongation by HIV-1 Tat". Science. 274 (5287): 605–610. Bibcode:1996Sci...274..605Z. doi:10.1126/science.274.5287.605. PMID   8849451. S2CID   13266489.
• Okamoto H, Sheline CT, Corden JL, et al. (1996). "Trans-activation by human immunodeficiency virus Tat protein requires the C-terminal domain of RNA polymerase II". Proc. Natl. Acad. Sci. U.S.A. 93 (21): 11575–11579. Bibcode:1996PNAS...9311575O. doi: 10.1073/pnas.93.21.11575 . PMC   38099 . PMID   8876177.
• Chun RF, Jeang KT (1996). "Requirements for RNA polymerase II carboxyl-terminal domain for activated transcription of human retroviruses human T-cell lymphotropic virus I and HIV-1". J. Biol. Chem. 271 (44): 27888–27894. doi: 10.1074/jbc.271.44.27888 . PMID   8910388.