Description
Thelocarpon immersum forms minute, flask-shaped fruiting bodies (ascomata) that remain almost completely buried in the lichen's algal mat. Each yellow-green ascoma measures only 0.08–0.12 mm across and pierces the surface just at its apex, giving the thallus a pitted appearance. The wall that surrounds the cavity ( excipulum ) is clear except for its uppermost 100 μm, which is tinged yellow-brown. Short, simple filaments called periphyses line the neck of the ascoma and project 30–40 μm into the pore. [2]
Under the microscope the spore-bearing layer (hymenium) reacts with iodine to a deep orange-brown (I+). Slender supporting filaments (paraphyses) thread the hymenium; they are 80–100 μm long and only about 1 μm wide, branching sparingly and occasionally fusing with each other. The spore sacs (asci) begin cylindrical (75–90 × 15–17 μm) with a narrow upper tip but later broaden into a club shape up to 20 μm wide; their walls stain orange-brown with iodine, while the interior contents stain yellow. Each ascus contains eight round ascospores, 5–7 μm in diameter, that also give a yellow iodine reaction. No asexual propagules have been observed, and all standard lichen spot tests are negative, indicating that the species lacks the common secondary metabolites often used in field identification. [2]
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