Translin

TSN
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 1J1J, 3PJA, 3QB5, 4WYV
Identifiers
Aliases	TSN , BCLF-1, C3PO, RCHF1, REHF-1, TBRBP, TRSLN, translin
External IDs	OMIM: 600575 MGI: 109263 HomoloGene: 3397 GeneCards: TSN
Gene location (Human) Chr. Chromosome 2 (human) [1] Band 2q14.3 Start 121,737,103 bp [1] End 121,767,853 bp [1]
Gene location (Mouse) Chr. Chromosome 1 (mouse) [2] Band 1|1 E2.3 Start 118,222,508 bp [2] End 118,239,469 bp [2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in secondary oocyte optic nerve ganglionic eminence thoracic diaphragm germinal epithelium islet of Langerhans superficial temporal artery hair follicle human penis endometrium Top expressed in trigeminal ganglion renal corpuscle primitive streak medullary collecting duct neural tube Paneth cell superior cervical ganglion barrel cortex ciliary body sexually immature organism More reference expression data BioGPS More reference expression data
Gene ontology Molecular function DNA binding nuclease activity endonuclease activity sequence-specific DNA binding protein binding mRNA binding hydrolase activity RNA binding single-stranded DNA binding identical protein binding endoribonuclease activity protein-containing complex binding Cellular component cytoplasm cytosol nucleus Biological process DNA recombination nucleic acid phosphodiester bond hydrolysis RNA metabolic process RNA phosphodiester bond hydrolysis, endonucleolytic production of siRNA involved in RNA interference Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 7247 22099 Ensembl ENSG00000211460 ENSMUSG00000026374 UniProt Q15631 Q62348 RefSeq (mRNA) NM_001261401 NM_004622 NM_011650 RefSeq (protein) NP_001248330 NP_004613 NP_035780 Location (UCSC) Chr 2: 121.74 – 121.77 Mb Chr 1: 118.22 – 118.24 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

Translin is a DNA-binding protein that in humans is encoded by the TSN gene. ^{[5] [6] [7]} Together with translin-associated factor X, translin forms the component 3 of promoter of RISC (C3PO) complex which facilitates endonucleolytic cleavage of the passenger strand during microRNA loading into the RNA-induced silencing complex (RISC). ^{[8] [9] [10]}

Function

This gene encodes a DNA-binding protein which specifically recognizes conserved target sequences at the breakpoint junction of chromosomal translocations. Translin polypeptides form a multimeric structure that is responsible for its DNA-binding activity. Recombination-associated motifs and translin-binding sites are present at recombination hotspots and may serve as indicators of breakpoints in genes which are fused by translocations. These binding activities may play a crucial role in chromosomal translocation in lymphoid neoplasms. ^[7]

Interactions

Translin has been shown to interact with PPP1R15A. ^[11]

References

1. GRCh38: Ensembl release 89: ENSG00000211460 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000026374 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Kasai M, Aoki K, Matsuo Y, Minowada J, Maziarz RT, Strominger JL (July 1994). "Recombination hotspot associated factors specifically recognize novel target sequences at the site of interchromosomal rearrangements in T-ALL patients with t(8;14)(q24;q11) and t(1;14)(p32;q11)". International Immunology . 6 (7): 1017–25. doi:10.1093/intimm/6.7.1017. PMID   7947454.
6. Aoki K, Inazawa J, Takahashi T, Nakahara K, Kasai M (July 1997). "Genomic structure and chromosomal localization of the gene encoding translin, a recombination hotspot binding protein". Genomics. 43 (2): 237–41. doi:10.1006/geno.1997.4796. PMID   9244443.
7. "Entrez Gene: TSN translin".
8. Liu Y, Ye X, Jiang F, Liang C, Chen D, Peng J, Kinch LN, Grishin NV, Liu Q (August 2009). "C3PO, an endoribonuclease that promotes RNAi by facilitating RISC activation". Science. 325 (5941): 750–3. Bibcode:2009Sci...325..750L. doi:10.1126/science.1176325. PMC   2855623 . PMID   19661431.
9. Ye X, Huang N, Liu Y, Paroo Z, Huerta C, Li P, Chen S, Liu Q, Zhang H (June 2011). "Structure of C3PO and mechanism of human RISC activation". Nature Structural & Molecular Biology. 18 (6): 650–7. doi:10.1038/nsmb.2032. PMC   3109212 . PMID   21552258.
10. Ha M, Kim VN (August 2014). "Regulation of microRNA biogenesis". Nature Reviews Molecular Cell Biology. 15 (8): 509–24. doi:10.1038/nrm3838. PMID   25027649. S2CID   205495632.
11. Hasegawa T, Isobe K (August 1999). "Evidence for the interaction between Translin and GADD34 in mammalian cells". Biochimica et Biophysica Acta (BBA) - General Subjects. 1428 (2–3): 161–8. doi:10.1016/s0304-4165(99)00060-4. PMID   10434033.

Further reading

• Aoki K, Suzuki K, Sugano T, Tasaka T, Nakahara K, Kuge O, Omori A, Kasai M (June 1995). "A novel gene, Translin, encodes a recombination hotspot binding protein associated with chromosomal translocations". Nature Genetics. 10 (2): 167–74. doi:10.1038/ng0695-167. PMID   7663511. S2CID   9678546.
• Maruyama K, Sugano S (January 1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID   8125298.
• Aoki K, Nakahara K, Ikegawa C, Seto M, Takahashi T, Minowada J, Strominger JL, Maziarz RT, Kasai M (April 1994). "Nuclear proteins binding to a novel target sequence within the recombination hotspot regions of Bcl-2 and the immunoglobulin DH gene family". Oncogene. 9 (4): 1109–15. PMID   8134113.
• Aoki K, Ishida R, Kasai M (January 1997). "Isolation and characterization of a cDNA encoding a Translin-like protein, TRAX". FEBS Letters. 401 (2–3): 109–12. doi:10.1016/S0014-5793(96)01444-5. PMID   9013868. S2CID   46704829.
• Kasai M, Matsuzaki T, Katayanagi K, Omori A, Maziarz RT, Strominger JL, Aoki K, Suzuki K (April 1997). "The translin ring specifically recognizes DNA ends at recombination hot spots in the human genome". The Journal of Biological Chemistry. 272 (17): 11402–7. doi: 10.1074/jbc.272.17.11402 . PMID   9111049.
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S (October 1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID   9373149.
• Hasegawa T, Isobe K (August 1999). "Evidence for the interaction between Translin and GADD34 in mammalian cells". Biochimica et Biophysica Acta (BBA) - General Subjects. 1428 (2–3): 161–8. doi:10.1016/s0304-4165(99)00060-4. PMID   10434033.
• Badge RM, Yardley J, Jeffreys AJ, Armour JA (May 2000). "Crossover breakpoint mapping identifies a subtelomeric hotspot for male meiotic recombination". Human Molecular Genetics. 9 (8): 1239–44. doi: 10.1093/hmg/9.8.1239 . PMID   10767349.
• Hosaka T, Kanoe H, Nakayama T, Murakami H, Yamamoto H, Nakamata T, Tsuboyama T, Oka M, Kasai M, Sasaki MS, Nakamura T, Toguchida J (November 2000). "Translin binds to the sequences adjacent to the breakpoints of the TLS and CHOP genes in liposarcomas with translocation t(12;6)". Oncogene. 19 (50): 5821–5. doi: 10.1038/sj.onc.1203943 . hdl: 2433/150205 . PMID   11126370.
• Chennathukuzhi VM, Kurihara Y, Bray JD, Hecht NB (April 2001). "Trax (translin-associated factor X), a primarily cytoplasmic protein, inhibits the binding of TB-RBP (translin) to RNA". The Journal of Biological Chemistry. 276 (16): 13256–63. doi: 10.1074/jbc.M009707200 . PMID   11278549.
• Lee SP, Fuior E, Lewis MS, Han MK (November 2001). "Analytical ultracentrifugation studies of translin: analysis of protein-DNA interactions using a single-stranded fluorogenic oligonucleotide". Biochemistry. 40 (46): 14081–8. doi:10.1021/bi010302t. PMID   11705401.
• Erdemir T, Bilican B, Oncel D, Goding CR, Yavuzer U (January 2002). "DNA damage-dependent interaction of the nuclear matrix protein C1D with Translin-associated factor X (TRAX)". Journal of Cell Science. 115 (Pt 1): 207–16. doi:10.1242/jcs.115.1.207. hdl: 11693/24740 . PMID   11801738.
• Han MK, Lin P, Paek D, Harvey JJ, Fuior E, Knutson JR (March 2002). "Fluorescence studies of pyrene maleimide-labeled translin: excimer fluorescence indicates subunits associate in a tail-to-tail configuration to form octamer". Biochemistry. 41 (10): 3468–76. doi:10.1021/bi015901e. PMID   11876655.
• Sengupta K, Rao BJ (December 2002). "Translin binding to DNA: recruitment through DNA ends and consequent conformational transitions". Biochemistry. 41 (51): 15315–26. doi:10.1021/bi026378m. PMID   12484770.
• Li K, Wang L, Cheng J, Lu YY, Zhang LX, Mu JS, Hong Y, Liu Y, Duan HJ, Wang G, Li L, Chen JM (February 2003). "Interaction between hepatitis C virus core protein and translin protein--a possible molecular mechanism for hepatocellular carcinoma and lymphoma caused by hepatitis C virus". World Journal of Gastroenterology. 9 (2): 300–3. doi: 10.3748/wjg.v9.i2.300 . PMC   4611333 . PMID   12532453.
• Yang S, Cho YS, Chennathukuzhi VM, Underkoffler LA, Loomes K, Hecht NB (March 2004). "Translin-associated factor X is post-transcriptionally regulated by its partner protein TB-RBP, and both are essential for normal cell proliferation". The Journal of Biological Chemistry. 279 (13): 12605–14. doi: 10.1074/jbc.M313133200 . PMID   14711818.

External links

• Overview of all the structural information available in the PDB for UniProt : Q15631 (Human Translin) at the PDBe-KB .
• Overview of all the structural information available in the PDB for UniProt : Q62348 (Mouse Translin) at the PDBe-KB .