# Trithionate hydrolase

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trithionate hydrolase
Identifiers
EC number 3.12.1.1
CAS number 115004-90-5
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MetaCyc metabolic pathway
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In enzymology, a trithionate hydrolase (EC 3.12.1.1) is an enzyme that catalyzes the chemical reaction [1] [2]

trithionate + H2O ${\displaystyle \rightleftharpoons }$ thiosulfate + sulfate + 2 H+

Thus, the two substrates of this enzyme are trithionate and H2O, whereas its 3 products are thiosulfate, sulfate, and H+.

This enzyme belongs to the family of hydrolases, specifically those acting on sulfur-sulfur bonds. The systematic name of this enzyme class is trithionate thiosulfohydrolase. This enzyme participates in sulfur metabolism.

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The genus Annwoodia was named in 2017 to circumscribe an organism previously described as a member of the genus Thiobacillus, Thiobacillus aquaesulis - the type and only species is Annwoodia aquaesulis, which was isolated from the geothermal waters of the Roman Baths in the city of Bath in the United Kingdom by Ann P. Wood and Donovan P. Kelly of the University of Warwick - the genus was subsequently named to honour Wood's contribution to microbiology. The genus falls within the family Thiobacillaceae along with Thiobacillus and Sulfuritortus, both of which comprise autotrophic organisms dependent on thiosulfate, other sulfur oxyanions and sulfide as electron donors for chemolithoheterotrophic growth. Whilst Annwoodia spp. and Sulfuritortus spp. are thermophilic, Thiobacillus spp. are mesophilic.

Microbial oxidation of sulfur is the oxidation of sulfur by microorganisms to produce energy. The oxidation of inorganic compounds is the strategy primarily used by chemolithotrophic microorganisms to obtain energy in order to build their structural components, survive, grow and reproduce. Some inorganic forms of reduced sulfur, mainly sulfide (H2S/HS) and elemental sulfur (S0), can be oxidized by chemolithotrophic sulfur-oxidizing prokaryotes, usually coupled to the reduction of oxygen (O2) or nitrate (NO3).

## References

1. Lu WP, Kelly DP (1988). "Cellular location and partial purification of the 'thiosulphate-oxidizing enzyme' and 'trithionate hydrolase' from Thiobacillus tepidarius". J. Gen. Microbiol. 134: 877–885. doi:.
2. Trudinger PA (1964). "The metabolism of trithionate by Thiobacillus X". Aust. J. Biol. Sci. 17: 459–468.