| 2010 Open Barletta | |
|---|---|
| Date | March 22 – March 28 |
| Edition | 14th |
| Location | Barletta, Italy |
| 2009 Champions | |
| Singles | |
 Pere Riba | |
| Doubles | |
| David Marrero / Santiago Ventura | |
The 2010 Open Barletta was a professional tennis tournament played on outdoor red clay courts. It was part of the 2010 ATP Challenger Tour. It took place in Barletta, Italy between 22 and 28 March 2010.
| Nationality | Player | Ranking* | Seeding |
|---|---|---|---|
 FIN | Jarkko Nieminen | 85 | 1 |
| ESP | Santiago Ventura | 91 | 2 |
 BEL | Steve Darcis | 99 | 3 |
 SLO | Blaž Kavčič | 101 | 4 |
 RUS | Igor Kunitsyn | 102 | 5 |
| ESP | Pere Riba | 117 | 6 |
| ESP | Iván Navarro | 123 | 7 |
 ITA | Simone Bolelli | 126 | 8 |
The following players received wildcards into the singles main draw:
The following players received entry from the qualifying draw:
Pere Riba def. Steve Darcis, 6–3, ret.
David Marrero / Santiago Ventura def. 
Ilija Bozoljac / Daniele Bracciali, 6–3, 6–3
