CIB1

CIB1
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 1DGU, 1DGV, 1XO5, 1Y1A, 2L4H, 2L4I, 2LM5
Identifiers
Aliases	CIB1 , CIB, CIBP, KIP1, PRKDCIP, SIP2-28, calcium and integrin binding 1
External IDs	OMIM: 602293 MGI: 1344418 HomoloGene: 4654 GeneCards: CIB1
Gene location (Human) Chr. Chromosome 15 (human) [1] Band 15q26.1 Start 90,229,975 bp [1] End 90,234,047 bp [1]
Gene location (Mouse) Chr. Chromosome 7 (mouse) [2] Band 7|7 D2 Start 79,876,895 bp [2] End 79,882,561 bp [2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in bronchial epithelial cell right uterine tube body of pancreas right adrenal gland left adrenal gland rectum minor salivary gland right lobe of liver right lobe of thyroid gland left lobe of thyroid gland Top expressed in spermatocyte spermatid duodenum epithelium of stomach yolk sac pyloric antrum jejunum crypt of lieberkuhn of small intestine seminiferous tubule left colon More reference expression data BioGPS More reference expression data
Gene ontology Molecular function protein serine/threonine kinase inhibitor activity calcium ion binding protein-membrane adaptor activity transmembrane transporter binding metal ion binding protein C-terminus binding protein binding calcium-dependent protein kinase inhibitor activity protein kinase binding Cellular component cytoplasm vesicle Golgi apparatus cell projection centrosome membrane growth cone plasma membrane nucleoplasm microtubule organizing center ruffle membrane axon filopodium tip dendrite neuronal cell body apical plasma membrane endoplasmic reticulum perinuclear region of cytoplasm neuron projection sarcolemma extracellular exosome cytoskeleton nucleus cell periphery lamellipodium Biological process negative regulation of protein phosphorylation positive regulation of protein targeting to membrane thrombopoietin-mediated signaling pathway negative regulation of protein kinase B signaling cell differentiation cellular response to nerve growth factor stimulus positive regulation of protein phosphorylation negative regulation of neuron projection development regulation of cell division positive regulation of cell migration involved in sprouting angiogenesis extrinsic apoptotic signaling pathway positive regulation of cell-matrix adhesion positive regulation of cell migration cellular response to growth factor stimulus cellular response to tumor necrosis factor platelet formation negative regulation of apoptotic process negative regulation of megakaryocyte differentiation positive regulation of protein serine/threonine kinase activity positive regulation of calcineurin-NFAT signaling cascade cell division cellular response to DNA damage stimulus negative regulation of microtubule depolymerization positive regulation of substrate adhesion-dependent cell spreading positive regulation of cell growth response to ischemia cell adhesion regulation of cell population proliferation positive regulation of NF-kappaB transcription factor activity double-strand break repair angiogenesis spermatogenesis positive regulation of cell population proliferation spermatid development positive regulation of ERK1 and ERK2 cascade cytoplasmic microtubule organization endomitotic cell cycle cell cycle positive regulation of cell adhesion mediated by integrin positive regulation of male germ cell proliferation negative regulation of cell population proliferation apoptotic process positive regulation of catalytic activity negative regulation of protein kinase activity negative regulation of protein serine/threonine kinase activity positive regulation of protein localization to plasma membrane Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 10519 23991 Ensembl ENSG00000185043 ENSMUSG00000030538 UniProt Q99828 Q9Z0F4 RefSeq (mRNA) NM_001277764 NM_006384 NM_001291275 NM_001291276 NM_011870 RefSeq (protein) NP_001264693 NP_006375 NP_001278204 NP_001278205 NP_036000 Location (UCSC) Chr 15: 90.23 – 90.23 Mb Chr 7: 79.88 – 79.88 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

Calcium and integrin-binding protein 1 is a protein that in humans is encoded by the CIB1 gene and is located in Chromosome 15. ^{[5] [6] [7]} The protein encoded by this gene is a member of the calcium-binding protein family. The specific function of this protein has not yet been determined; however this protein is known to interact with DNA-dependent protein kinase and may play a role in kinase-phosphatase regulation of DNA end-joining. This protein also interacts with integrin alpha(IIb)beta(3), which may implicate this protein as a regulatory molecule for alpha(IIb)beta(3). ^[7]

Structure and function

CIB1 is a small protein with a molecular weight of approximately 22 kDa. It has a conserved calcium-binding EF hand domain, which consists of two alpha-helices connected by a loop. ^{[8] [9]} CIB1 also has an integrin-binding domain, located near the N-terminus of the protein. In addition, CIB1 has a coiled-coil domain and a C-terminal domain. ^{[8] [10] [11]} CIB1 is involved in regulating cell adhesion, migration, and differentiation, as well as other cellular processes. It interacts with integrins, which are transmembrane receptors that play a key role in cell signaling and adhesion to the extracellular matrix. CIB1 has also been shown to regulate other signaling pathways that are important for cell survival and proliferation. ^[8] Upregulation of CIB1 expression has been observed in several types of cancer, and it has been implicated in cancer development and progression. ^[8] CIB1 is involved in several cellular processes that are important for cancer progression, including cell adhesion, migration, and invasion. It has been shown to interact with integrins, which are transmembrane receptors that play a key role in these processes. ^[8] The structure and function of CIB1 make it an important protein in regulating various cellular processes, including those involved in cancer progression, and targeting it may offer potential therapeutic benefits.

Cancer

CIB1 expression has been observed in several types of cancer, including breast, lung, prostate, ovarian, and pancreatic cancer. In breast cancer, CIB1 expression has been shown to be higher in invasive ductal carcinoma compared to normal breast tissue. ^[12] High levels of CIB1 expression have also been associated with poor prognosis in breast cancer patients. ^[12] CIB1 has been implicated in cancer development and progression. In breast cancer, CIB1 has been shown to promote cell proliferation, migration, invasion, and metastasis. ^[13] CIB1 has also been shown to promote the growth of prostate cancer cells and the invasion of ovarian cancer cells. ^[13] Targeting CIB1 has been explored as a potential therapeutic strategy for cancer. Small molecule inhibitors of CIB1 have shown promise in preclinical models of breast cancer. Silencing CIB1 expression has also been shown to sensitize cancer cells to chemotherapy and radiation therapy. ^{[14] [13]}

Male infertility

CIB1 has been implicated in male fertility, specifically in sperm function and motility. ^[15] CIB1 expression has been detected in human sperm, and its levels have been correlated with sperm motility. CIB1 has also been shown to be present in the acrosome region of the sperm, which plays a critical role in fertilization. Studies in mice have shown that CIB1 deficiency leads to impaired sperm motility and reduced fertility. ^{[8] [15]} Male mice lacking CIB1 exhibited decreased sperm count and decreased sperm motility, resulting in reduced fertility. CIB1 was also found to be required for the proper formation of the sperm tail, which is critical for sperm motility. ^[8] In addition, CIB1 has been shown to regulate calcium signaling in sperm, which is important for sperm motility and fertilization. CIB1 interacts with the sperm-specific calcium channel CatSper, which is important for regulating intracellular calcium levels in sperm. ^[8]

Spermatogenesis

Spermatogenesis is the process of producing mature spermatozoa from spermatogonia, the precursor cells in the testes. This process involves several stages, including mitotic division, meiotic division, and differentiation, which results in the production of four haploid sperm cells from one diploid spermatogonium. CIB1 has been shown to play a critical role in spermatogenesis by regulating the differentiation of spermatogonia into spermatocytes. Studies have shown that CIB1 is expressed in spermatogonia, spermatocytes, and spermatids, indicating its role throughout the entire process of spermatogenesis. ^{[8] [15]} In mice, CIB1 deficiency has been shown to lead to decreased spermatogonia proliferation and impaired differentiation into spermatocytes, resulting in reduced sperm production and male infertility. ^[15] In addition, CIB1 has been shown to regulate the expression of genes involved in spermatogenesis, including genes involved in cell proliferation and differentiation. ^{[8] [15]}

Interactions

CIB1 has been shown to interact with RAC3, ^[16] PSEN2, ^[17] DNA-PKcs, ^[18] UBR5 ^[19] and CD61. ^[5]

References

1. GRCh38: Ensembl release 89: ENSG00000185043 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000030538 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Naik UP, Patel PM, Parise LV (Apr 1997). "Identification of a novel calcium-binding protein that interacts with the integrin alphaIIb cytoplasmic domain". J Biol Chem. 272 (8): 4651–4. doi: 10.1074/jbc.272.8.4651 . PMID   9030514.
6. Hattori A, Seki N, Hayashi A, Kozuma S, Saito T (Aug 2000). "Genomic structure of mouse and human genes for DNA-PKcs interacting protein (KIP)". DNA Seq. 10 (6): 415–8. doi:10.3109/10425170009015612. PMID   10826701. S2CID   21570442.
7. "Entrez Gene: CIB1 calcium and integrin binding 1 (calmyrin)".
8. Leisner TM, Freeman TC, Black JL, Parise LV (August 2016). "CIB1: a small protein with big ambitions". The FASEB Journal. 30 (8): 2640–2650. doi:10.1096/fj.201500073R. ISSN   0892-6638. PMC   4970603 . PMID   27118676.
9. Kretsinger RH, Nockolds CE (1973-05-10). "Carp muscle calcium-binding protein. II. Structure determination and general description". The Journal of Biological Chemistry. 248 (9): 3313–3326. doi: 10.1016/S0021-9258(19)44043-X . ISSN   0021-9258. PMID   4700463.
10. Blamey CJ, Ceccarelli C, Naik UP, Bahnson BJ (May 2005). "The crystal structure of calcium- and integrin-binding protein 1: Insights into redox regulated functions". Protein Science. 14 (5): 1214–1221. doi:10.1110/ps.041270805. ISSN   0961-8368. PMC   2253279 . PMID   15840829.
11. Huang H, Ishida H, Yamniuk AP, Vogel HJ (May 2011). "Solution Structures of Ca2+-CIB1 and Mg2+-CIB1 and Their Interactions with the Platelet Integrin αIIb Cytoplasmic Domain". Journal of Biological Chemistry. 286 (19): 17181–17192. doi: 10.1074/jbc.m110.179028 . ISSN   0021-9258. PMC   3089561 . PMID   21388953.
12. Black JL, Harrell JC, Leisner TM, Fellmeth MJ, George SD, Reinhold D, Baker NM, Jones CD, Der CJ, Perou CM, Parise LV (2015-06-24). "CIB1 depletion impairs cell survival and tumor growth in triple-negative breast cancer". Breast Cancer Research and Treatment. 152 (2): 337–346. doi:10.1007/s10549-015-3458-4. ISSN   0167-6806. PMC   4516161 . PMID   26105795.
13. Liu Y, Zhou Y, Zhang P, Li X, Duan C, Zhang C (March 2021). "CHIP-mediated CIB1 ubiquitination regulated epithelial–mesenchymal transition and tumor metastasis in lung adenocarcinoma". Cell Death & Differentiation. 28 (3): 1026–1040. doi:10.1038/s41418-020-00635-5. ISSN   1476-5403. PMC   7937682 . PMID   33082516.
14. Qin X, Sun L, Wang J (2017-08-17). "Restoration of microRNA-708 sensitizes ovarian cancer cells to cisplatin via IGF2BP1/Akt pathway". Cell Biology International. 41 (10): 1110–1118. doi:10.1002/cbin.10819. ISSN   1065-6995. PMID   28685895. S2CID   4124914.
15. Yuan W, Leisner TM, McFadden AW, Clark S, Hiller S, Maeda N, O'Brien DA, Parise LV (2006-11-01). "CIB1 Is Essential for Mouse Spermatogenesis". Molecular and Cellular Biology. 26 (22): 8507–8514. doi:10.1128/mcb.01488-06. ISSN   1098-5549. PMC   1636792 . PMID   16982698.
16. Haataja L, Kaartinen Vesa, Groffen John, Heisterkamp Nora (Mar 2002). "The small GTPase Rac3 interacts with the integrin-binding protein CIB and promotes integrin alpha(IIb)beta(3)-mediated adhesion and spreading". J. Biol. Chem. 277 (10): 8321–8. doi: 10.1074/jbc.M105363200 . ISSN   0021-9258. PMID   11756406.
17. Stabler SM, Ostrowski L L, Janicki S M, Monteiro M J (Jun 1999). "A myristoylated calcium-binding protein that preferentially interacts with the Alzheimer's disease presenilin 2 protein". J. Cell Biol. 145 (6): 1277–92. doi:10.1083/jcb.145.6.1277. ISSN   0021-9525. PMC   2133148 . PMID   10366599.
18. Wu X, Lieber M R (Oct 1997). "Interaction between DNA-dependent protein kinase and a novel protein, KIP". Mutat. Res. 385 (1): 13–20. doi:10.1016/s0921-8777(97)00035-9. ISSN   0027-5107. PMID   9372844.
19. Henderson MJ, Russell Amanda J, Hird Samantha, Muñoz Marcia, Clancy Jennifer L, Lehrbach Gillian M, Calanni Sophina T, Jans David A, Sutherland Robert L, Watts Colin K W (Jul 2002). "EDD, the human hyperplastic discs protein, has a role in progesterone receptor coactivation and potential involvement in DNA damage response". J. Biol. Chem. 277 (29): 26468–78. doi: 10.1074/jbc.M203527200 . hdl: 1885/64590 . ISSN   0021-9258. PMID   12011095.

Further reading

• Wu X, Lieber MR (1997). "Interaction between DNA-dependent protein kinase and a novel protein, KIP". Mutat. Res. 385 (1): 13–20. doi:10.1016/s0921-8777(97)00035-9. PMID   9372844.
• Seki N, Hayashi A, Abe M, et al. (1999). "Chromosomal assignment of the gene for human DNA-PKcs interacting protein (KIP) on chromosome 15q25.3-q26.1 by somatic hybrid analysis and fluorescence in situ hybridization". J. Hum. Genet. 43 (4): 275–7. doi: 10.1007/s100380050089 . PMID   9852683.
• Stabler SM, Ostrowski LL, Janicki SM, Monteiro MJ (1999). "A myristoylated calcium-binding protein that preferentially interacts with the Alzheimer's disease presenilin 2 protein". J. Cell Biol. 145 (6): 1277–92. doi:10.1083/jcb.145.6.1277. PMC   2133148 . PMID   10366599.
• Kauselmann G, Weiler M, Wulff P, et al. (1999). "The polo-like protein kinases Fnk and Snk associate with a Ca(2+)- and integrin-binding protein and are regulated dynamically with synaptic plasticity". EMBO J. 18 (20): 5528–39. doi:10.1093/emboj/18.20.5528. PMC   1171621 . PMID   10523297.
• Hwang PM, Vogel HJ (2000). "Structures of the platelet calcium- and integrin-binding protein and the alphaIIb-integrin cytoplasmic domain suggest a mechanism for calcium-regulated recognition; homology modelling and NMR studies". J. Mol. Recognit. 13 (2): 83–92. doi:10.1002/(SICI)1099-1352(200003/04)13:2<83::AID-JMR491>3.0.CO;2-A. PMID   10822252. S2CID   42776144.
• Holtrich U, Wolf G, Yuan J, et al. (2000). "Adhesion induced expression of the serine/threonine kinase Fnk in human macrophages". Oncogene. 19 (42): 4832–9. doi:10.1038/sj.onc.1203845. PMID   11039900. S2CID   25807289.
• Haataja L, Kaartinen V, Groffen J, Heisterkamp N (2002). "The small GTPase Rac3 interacts with the integrin-binding protein CIB and promotes integrin alpha(IIb)beta(3)-mediated adhesion and spreading". J. Biol. Chem. 277 (10): 8321–8. doi: 10.1074/jbc.M105363200 . PMID   11756406.
• Whitehouse C, Chambers J, Howe K, et al. (2002). "NBR1 interacts with fasciculation and elongation protein zeta-1 (FEZ1) and calcium and integrin binding protein (CIB) and shows developmentally restricted expression in the neural tube". Eur. J. Biochem. 269 (2): 538–45. doi: 10.1046/j.0014-2956.2001.02681.x . PMID   11856312.
• Hollenbach AD, McPherson CJ, Lagutina I, Grosveld G (2002). "The EF-hand calcium-binding protein calmyrin inhibits the transcriptional and DNA-binding activity of Pax3". Biochim. Biophys. Acta. 1574 (3): 321–8. doi:10.1016/s0167-4781(02)00230-0. PMID   11997098.
• Henderson MJ, Russell AJ, Hird S, et al. (2002). "EDD, the human hyperplastic discs protein, has a role in progesterone receptor coactivation and potential involvement in DNA damage response". J. Biol. Chem. 277 (29): 26468–78. doi: 10.1074/jbc.M203527200 . hdl: 1885/64590 . PMID   12011095.
• Barry WT, Boudignon-Proudhon C, Shock DD, et al. (2002). "Molecular basis of CIB binding to the integrin alpha IIb cytoplasmic domain". J. Biol. Chem. 277 (32): 28877–83. doi: 10.1074/jbc.M202983200 . PMID   12023286.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi: 10.1073/pnas.242603899 . PMC   139241 . PMID   12477932.
• Ma S, Liu MA, Yuan YL, Erikson RL (2003). "The serum-inducible protein kinase Snk is a G1 phase polo-like kinase that is inhibited by the calcium- and integrin-binding protein CIB". Mol. Cancer Res. 1 (5): 376–84. PMID   12651910.
• Naik UP, Naik MU (2003). "Association of CIB with GPIIb/IIIa during outside-in signaling is required for platelet spreading on fibrinogen". Blood. 102 (4): 1355–62. doi: 10.1182/blood-2003-02-0591 . PMID   12714504.
• Naik MU, Naik UP (2004). "Calcium-and integrin-binding protein regulates focal adhesion kinase activity during platelet spreading on immobilized fibrinogen". Blood. 102 (10): 3629–36. doi: 10.1182/blood-2003-05-1703 . PMID   12881299. S2CID   41760625.
• Yamniuk AP, Nguyen LT, Hoang TT, Vogel HJ (2004). "Metal ion binding properties and conformational states of calcium- and integrin-binding protein". Biochemistry. 43 (9): 2558–68. doi:10.1021/bi035432b. PMID   14992593.
• Lee GE, Yu EY, Cho CH, et al. (2004). "DNA-protein kinase catalytic subunit-interacting protein KIP binds telomerase by interacting with human telomerase reverse transcriptase". J. Biol. Chem. 279 (33): 34750–5. doi: 10.1074/jbc.M401843200 . PMID   15190070.
• Lehner B, Sanderson CM (2004). "A protein interaction framework for human mRNA degradation". Genome Res. 14 (7): 1315–23. doi:10.1101/gr.2122004. PMC   442147 . PMID   15231747.

External links

• Human CIB1 genome location and CIB1 gene details page in the UCSC Genome Browser .