EXOC4

EXOC4
Identifiers
Aliases	EXOC4 , SEC8, SEC8L1, Sec8p, exocyst complex component 4
External IDs	OMIM: 608185 MGI: 1096376 HomoloGene: 40654 GeneCards: EXOC4
Gene location (Human) Chr. Chromosome 7 (human) [1] Band 7q33 Start 133,253,073 bp [1] End 134,066,589 bp [1]
Gene location (Mouse) Chr. Chromosome 6 (mouse) [2] Band 6 A3.3|6 14.59 cM Start 33,226,020 bp [2] End 33,950,914 bp [2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in bone marrow cells Achilles tendon ganglionic eminence deltoid muscle sural nerve tibialis anterior muscle islet of Langerhans thymus vastus lateralis muscle sperm Top expressed in fossa internal carotid artery external carotid artery condyle calvaria substantia nigra triceps brachii muscle pineal gland sternocleidomastoid muscle vas deferens More reference expression data BioGPS More reference expression data
Gene ontology Molecular function PDZ domain binding protein binding protein N-terminus binding Cellular component cytoplasm myelin sheath abaxonal region microvillus cytosol plasma membrane membrane exocyst growth cone membrane synapse cell projection Flemming body Biological process protein transport paraxial mesoderm formation regulation of macroautophagy vesicle docking involved in exocytosis protein targeting to membrane exocytosis Golgi to plasma membrane transport vesicle targeting chemical synaptic transmission transport vesicle tethering involved in exocytosis Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 60412 20336 Ensembl ENSG00000131558 ENSMUSG00000029763 UniProt Q96A65 O35382 RefSeq (mRNA) NM_001037126 NM_021807 NM_009148 NM_001347089 RefSeq (protein) NP_001032203 NP_068579 NP_001334018 NP_033174 Location (UCSC) Chr 7: 133.25 – 134.07 Mb Chr 6: 33.23 – 33.95 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

Exocyst complex component 4 is a protein that in humans is encoded by the EXOC4 gene. ^{[5] [6] [7]}

Function

The protein encoded by this gene is a component of the exocyst complex, a multiple protein complex essential for targeting exocytic vesicles to specific docking sites on the plasma membrane. Though best characterized in yeast, the component proteins and functions of exocyst complex have been demonstrated to be highly conserved in higher eukaryotes. At least eight components of the exocyst complex, including this protein, are found to interact with the actin cytoskeletal remodeling and vesicle transport machinery. The complex is also essential for the biogenesis of epithelial cell surface polarity. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. ^[7]

Interactions

EXOC4 has been shown to interact with:

• DLG3, ^[8]
• DLG4, ^{[8] [9]}
• EXOC3, ^{[6] [8]}
• EXOC7, ^{[6] [8]} and
• GRIN2B. ^[8]

References

1. GRCh38: Ensembl release 89: ENSG00000131558 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000029763 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Nagase T, Kikuno R, Hattori A, Kondo Y, Okumura K, Ohara O (Feb 2001). "Prediction of the coding sequences of unidentified human genes. XIX. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro". DNA Res. 7 (6): 347–55. doi: 10.1093/dnares/7.6.347 . PMID   11214970.
6. Inoue M, Chang L, Hwang J, Chiang SH, Saltiel AR (Apr 2003). "The exocyst complex is required for targeting of Glut4 to the plasma membrane by insulin" (PDF). Nature. 422 (6932): 629–33. Bibcode:2003Natur.422..629I. doi:10.1038/nature01533. hdl: 2027.42/62982 . PMID   12687004. S2CID   4395760.
7. "Entrez Gene: EXOC4 exocyst complex component 4".
8. Sans N, Prybylowski K, Petralia RS, Chang K, Wang YX, Racca C, Vicini S, Wenthold RJ (Jun 2003). "NMDA receptor trafficking through an interaction between PDZ proteins and the exocyst complex". Nat. Cell Biol. 5 (6): 520–30. doi:10.1038/ncb990. PMID   12738960. S2CID   13444388.
9. Riefler GM, Balasingam G, Lucas KG, Wang S, Hsu SC, Firestein BL (Jul 2003). "Exocyst complex subunit sec8 binds to postsynaptic density protein-95 (PSD-95): a novel interaction regulated by cypin (cytosolic PSD-95 interactor)". Biochem. J. 373 (Pt 1): 49–55. doi:10.1042/BJ20021838. PMC   1223477 . PMID   12675619.

Further reading

• Hsu SC, TerBush D, Abraham M, Guo W (2004). "The exocyst complex in polarized exocytosis". Int. Rev. Cytol. International Review of Cytology. 233: 243–65. doi:10.1016/S0074-7696(04)33006-8. ISBN   978-0-12-364637-8. PMID   15037366.
• Kee Y, Yoo JS, Hazuka CD, Peterson KE, Hsu SC, Scheller RH (1998). "Subunit structure of the mammalian exocyst complex". Proc. Natl. Acad. Sci. U.S.A. 94 (26): 14438–43. Bibcode:1997PNAS...9414438K. doi: 10.1073/pnas.94.26.14438 . PMC   25013 . PMID   9405631.
• Grindstaff KK, Yeaman C, Anandasabapathy N, Hsu SC, Rodriguez-Boulan E, Scheller RH, Nelson WJ (1998). "Sec6/8 complex is recruited to cell-cell contacts and specifies transport vesicle delivery to the basal-lateral membrane in epithelial cells". Cell. 93 (5): 731–40. doi: 10.1016/S0092-8674(00)81435-X . PMID   9630218. S2CID   6747965.
• Hsu SC, Hazuka CD, Roth R, Foletti DL, Heuser J, Scheller RH (1998). "Subunit composition, protein interactions, and structures of the mammalian brain sec6/8 complex and septin filaments". Neuron. 20 (6): 1111–22. doi: 10.1016/S0896-6273(00)80493-6 . PMID   9655500. S2CID   10915358.
• Hartley JL, Temple GF, Brasch MA (2001). "DNA cloning using in vitro site-specific recombination". Genome Res. 10 (11): 1788–95. doi:10.1101/gr.143000. PMC   310948 . PMID   11076863.
• Wiemann S, Weil B, Wellenreuther R, Gassenhuber J, Glassl S, Ansorge W, Böcher M, Blöcker H, Bauersachs S, Blum H, Lauber J, Düsterhöft A, Beyer A, Köhrer K, Strack N, Mewes HW, Ottenwälder B, Obermaier B, Tampe J, Heubner D, Wambutt R, Korn B, Klein M, Poustka A (2001). "Toward a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs". Genome Res. 11 (3): 422–35. doi:10.1101/gr.GR1547R. PMC   311072 . PMID   11230166.
• Simpson JC, Wellenreuther R, Poustka A, Pepperkok R, Wiemann S (2001). "Systematic subcellular localization of novel proteins identified by large-scale cDNA sequencing". EMBO Rep. 1 (3): 287–92. doi:10.1093/embo-reports/kvd058. PMC   1083732 . PMID   11256614.
• Brymora A, Valova VA, Larsen MR, Roufogalis BD, Robinson PJ (2001). "The brain exocyst complex interacts with RalA in a GTP-dependent manner: identification of a novel mammalian Sec3 gene and a second Sec15 gene". J. Biol. Chem. 276 (32): 29792–7. doi: 10.1074/jbc.C100320200 . PMID   11406615.
• Polzin A, Shipitsin M, Goi T, Feig LA, Turner TJ (2002). "Ral-GTPase influences the regulation of the readily releasable pool of synaptic vesicles". Mol. Cell. Biol. 22 (6): 1714–22. doi:10.1128/MCB.22.6.1714-1722.2002. PMC   135608 . PMID   11865051.
• Vega IE, Hsu SC (2003). "The septin protein Nedd5 associates with both the exocyst complex and microtubules and disruption of its GTPase activity promotes aberrant neurite sprouting in PC12 cells". NeuroReport. 14 (1): 31–7. doi:10.1097/00001756-200301200-00006. PMID   12544826. S2CID   22757290.
• Riefler GM, Balasingam G, Lucas KG, Wang S, Hsu SC, Firestein BL (2003). "Exocyst complex subunit sec8 binds to postsynaptic density protein-95 (PSD-95): a novel interaction regulated by cypin (cytosolic PSD-95 interactor)". Biochem. J. 373 (Pt 1): 49–55. doi:10.1042/BJ20021838. PMC   1223477 . PMID   12675619.
• Sans N, Prybylowski K, Petralia RS, Chang K, Wang YX, Racca C, Vicini S, Wenthold RJ (2003). "NMDA receptor trafficking through an interaction between PDZ proteins and the exocyst complex". Nat. Cell Biol. 5 (6): 520–30. doi:10.1038/ncb990. PMID   12738960. S2CID   13444388.
• Moskalenko S, Tong C, Rosse C, Mirey G, Formstecher E, Daviet L, Camonis J, White MA (2004). "Ral GTPases regulate exocyst assembly through dual subunit interactions". J. Biol. Chem. 278 (51): 51743–8. doi: 10.1074/jbc.M308702200 . PMID   14525976.
• Bouwmeester T, Bauch A, Ruffner H, Angrand PO, Bergamini G, Croughton K, Cruciat C, Eberhard D, Gagneur J, Ghidelli S, Hopf C, Huhse B, Mangano R, Michon AM, Schirle M, Schlegl J, Schwab M, Stein MA, Bauer A, Casari G, Drewes G, Gavin AC, Jackson DB, Joberty G, Neubauer G, Rick J, Kuster B, Superti-Furga G (2004). "A physical and functional map of the human TNF-alpha/NF-kappa B signal transduction pathway". Nat. Cell Biol. 6 (2): 97–105. doi:10.1038/ncb1086. PMID   14743216. S2CID   11683986.