Haloarcula marismortui

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Haloarcula marismortui
Scientific classification
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H. marismortui
Binomial name
Haloarcula marismortui
(ex Volcani 1940) Oren et al. 1990 [1]

Haloarcula marismortui is a halophilic archaeon isolated from the Dead Sea

Contents

Morphology

Haloarcula marismortui is a Gram-negative archaeon with a cell size of 1.0–2.x 2.0–3.0 μm (diameter x length). Cells are pleomorphic appearing as short rods to rectangles. H. marismortui is motile via archaellum and possesses a cell membrane that consists of triglycosyl, diether lipids, and glycoproteins. [2]

Metabolism

H. marismortui is an aerobic chemoorganotroph that utilizes glycolysis and a modified Entner-Doudaroff pathway for the breakdown of nutrients. H. marismortui utilizes energy sources such as glucose, sucrose, fructose, glycerol, malate, acetate & succinate while producing nitrogen, metabolic carbon, and acid as byproducts. Can also grow anaerobically by using nitrate as an electron acceptor. [2]

Genomic Properties

The genome of H. marismortui is organized into nine circular replicons, in which individual G+C content varies from 54 to 62%. [3] H. marismortui contains 4,366 genes and 4,274,642 base pairs (Strain ATCC 43049). [4]

H. marismortui has one of the only two prokaryotic large ribosomal subunits which have so far been crystallized. The other one is Deinococcus radiodurans.

Ecology

Habitat

Haloarcula marismortui is considered an extreme halophile and has been isolated from the Dead Sea. [3] H. marismortui has a temperature optima between 40 and 50 °C and a pH range of 5.5–8.0. Growth can occur at a wide range of NaCl concentrations spanning 5-35% with optimal growth between 15 and 25%. [2] The unusually large number of environmental regulatory genes found within the H. marismortui genome suggests higher fitness in extreme environments compared to other species of Halobacterium. [3]

Adaptability

H. marismortui encodes a large family of multi-domain proteins(49) that act as sensors and regulators including Opsin proteins "Sop I & II, Hop, & Bop". These proteins help maintain physiological ion concentrations, facilitate phototaxis, and generate chemical energy via a proton gradient. [3] H. marismortui is believed to possess over 100 ecoparalogs, genes that perform the same function under environmental stress, that helps maintain its system of environmental adaptability. Multiple genes were found to have a factor on temperature control (rrnA/B/C) and cell motility (FlaA2 & FlaB). [5] H. marismortui encodes a large family of multidomain proteins (49) that act as environmental regulators and sensors. [3] This allows H. marimortui to survive in highly variable environmental conditions.

High environmental adaptability makes H. marismortui an ideal candidate for future bioremediation research with the potential of utilizing its environmental sensory genes in environmental clean up. [3]

Related Research Articles

A halophile is an extremophile that thrives in high salt concentrations. In chemical terms, halophile refers to a Lewis acidic species that has some ability to extract halides from other chemical species.

Archaeoglobus is a genus of the phylum Euryarchaeota. Archaeoglobus can be found in high-temperature oil fields where they may contribute to oil field souring.

Methanopyrus is a genus of methanogen, with a single described species, Methanopyrus kandleri. It is a rod-shaped hyperthermophile, discovered on the wall of a black smoker from the Gulf of California at a depth of 2,000 m, at temperatures of 84–110 °C. Strain 116 was discovered in black smoker fluid of the Kairei hydrothermal field; it can survive and reproduce at 122 °C. M. kandleri also requires a high ionic concentration in order for growth and cellular activity. Due to the species' high resilience and extreme environment, M. kandleri is also classified as an extremophile. It lives in a hydrogen-carbon dioxide rich environment, and like other methanogens reduces the latter to methane. It is placed among the Euryarchaeota, in its own class.

<i>Halobacterium</i> Genus of archaea

Halobacterium is a genus in the family Halobacteriaceae.

<i>Halobacterium salinarum</i> Species of archaeon

Halobacterium salinarum, formerly known as Halobacterium cutirubrum or Halobacterium halobium, is an extremely halophilic marine obligate aerobic archaeon. Despite its name, this is not a bacterium, but a member of the domain Archaea. It is found in salted fish, hides, hypersaline lakes, and salterns. As these salterns reach the minimum salinity limits for extreme halophiles, their waters become purple or reddish color due to the high densities of halophilic Archaea. H. salinarum has also been found in high-salt food such as salt pork, marine fish, and sausages. The ability of H. salinarum to survive at such high salt concentrations has led to its classification as an extremophile.

<i>Haloarcula</i> Genus of archaea

Haloarcula is a genus of extreme halophilic Archaea in the class of Halobactaria.

<i>Haloferax</i> Genus of archaea

In taxonomy, Haloferax is a genus of the Haloferacaceae.

In taxonomy, Natrialba is a genus of the Natrialbaceae. The genus consists of many diverse species that can survive extreme environmental niches, especially they are capable to live in the waters saturated or nearly saturated with salt (halophiles). They have certain adaptations to live within their salty environments. For example, their cellular machinery is adapted to high salt concentrations by having charged amino acids on their surfaces, allowing the cell to keep its water molecules around these components. The osmotic pressure and these amino acids help to control the amount of salt within the cell.

<span class="mw-page-title-main">Two-component regulatory system</span>

In the field of molecular biology, a two-component regulatory system serves as a basic stimulus-response coupling mechanism to allow organisms to sense and respond to changes in many different environmental conditions. Two-component systems typically consist of a membrane-bound histidine kinase that senses a specific environmental stimulus and a corresponding response regulator that mediates the cellular response, mostly through differential expression of target genes. Although two-component signaling systems are found in all domains of life, they are most common by far in bacteria, particularly in Gram-negative and cyanobacteria; both histidine kinases and response regulators are among the largest gene families in bacteria. They are much less common in archaea and eukaryotes; although they do appear in yeasts, filamentous fungi, and slime molds, and are common in plants, two-component systems have been described as "conspicuously absent" from animals.

<span class="mw-page-title-main">60S ribosomal protein L17</span> Protein found in humans

Large ribosomal subunit protein uL22 is a protein that in humans is encoded by the RPL17 gene.

Archaeocin is the name given to a new type of potentially useful antibiotic that is derived from the Archaea group of organisms. Eight archaeocins have been partially or fully characterized, but hundreds of archaeocins are believed to exist, especially within the haloarchaea. Production of these archaeal proteinaceous antimicrobials is a nearly universal feature of the rod-shaped haloarchaea.

<i>Halspiviridae</i> Family of viruses

Halspiviridae is a family of viruses that consists of a single genus, Salterprovirus, which consists of a single recognised species; Salterprovirus His1. This virus was isolated from hypersaline water in Australia and was able to be cultured on the halophilic archaeon Haloarcula hispanica. Like many other archaeoviruses, His1 has an approximately limoniform (lemon-shaped) virion.

The archaellum is a unique structure on the cell surface of many archaea that allows for swimming motility. The archaellum consists of a rigid helical filament that is attached to the cell membrane by a molecular motor. This molecular motor – composed of cytosolic, membrane, and pseudo-periplasmic proteins – is responsible for the assembly of the filament and, once assembled, for its rotation. The rotation of the filament propels archaeal cells in liquid medium, in a manner similar to the propeller of a boat. The bacterial analog of the archaellum is the flagellum, which is also responsible for their swimming motility and can also be compared to a rotating corkscrew. Although the movement of archaella and flagella is sometimes described as "whip-like", this is incorrect, as only cilia from Eukaryotes move in this manner. Indeed, even "flagellum" is a misnomer, as bacterial flagella also work as propeller-like structures.

<i>Haloferax volcanii</i> Species of Halobacteria

Haloferax volcanii is a species of organism in the genus Haloferax in the Archaea.

<i>Haloquadratum walsbyi</i> Species of halotolerant archaea

Haloquadratum walsbyi is of the genus Haloquadratum, within the archaea domain known for its square halophilic nature. First discovered in a brine pool in the Sinai peninsula of Egypt, H. walsbyi is noted for its flat, square-shaped cells, and its unusual ability to survive in aqueous environments with high concentrations of sodium chloride and magnesium chloride. The species' genus name Haloquadratum translates from Greek and Latin as "salt square". This archaean is also commonly referred to as "Walsby's Square Bacterium" because of its identifying square shape which makes it unique. In accordance with its name, Haloquadratum walsbyi are most abundantly observed in salty environments.

Halobacterium noricense is a halophilic, rod-shaped microorganism that thrives in environments with salt levels near saturation. Despite the implication of the name, Halobacterium is actually a genus of archaea, not bacteria. H. noricense can be isolated from environments with high salinity such as the Dead Sea and the Great Salt Lake in Utah. Members of the Halobacterium genus are excellent model organisms for DNA replication and transcription due to the stability of their proteins and polymerases when exposed to high temperatures. To be classified in the genus Halobacterium, a microorganism must exhibit a membrane composition consisting of ether-linked phosphoglycerides and glycolipids.

Halostagnicola larsenii is a non-motile, aerobic, gram-negative, rod shaped archaeon. It is a halophilic, neutrophilic, chemo-organotroph and was isolated from samples taken from a saline lake in China. The etymology of the name comes from hals, halos Greek for salt, stagnum Latin for a piece of standing water, -cola Latin for inhabitant or dweller, and Larsenii named after the Norwegian microbiologist, Helge Larsen, who was a pioneer in research regarding halophiles.

Acidilobus saccharovorans is a thermoacidophilic species of anaerobic archaea. The species was originally described in 2009 after being isolated from hot springs in Kamchatka.

<i>Halorubrum lacusprofundi</i> Species of archaeon

Halorubrum lacusprofundi is a rod-shaped, halophilic Archaeon in the family of Halorubraceae. It was first isolated from Deep Lake in Antarctica in the 1980s.

<span class="mw-page-title-main">Archaeal virus</span>

An archaeal virus is a virus that infects and replicates in archaea, a domain of unicellular, prokaryotic organisms. Archaeal viruses, like their hosts, are found worldwide, including in extreme environments inhospitable to most life such as acidic hot springs, highly saline bodies of water, and at the bottom of the ocean. They have been also found in the human body. The first known archaeal virus was described in 1974 and since then, a large diversity of archaeal viruses have been discovered, many possessing unique characteristics not found in other viruses. Little is known about their biological processes, such as how they replicate, but they are believed to have many independent origins, some of which likely predate the last archaeal common ancestor (LACA).

References

  1. "LPSN - List of Prokaryotic names with Standing in Nomenclature". Deutsche Sammlung von Mikroorganismen und Zellkulturen . Retrieved 2022-07-12.
  2. 1 2 3 Oren, Aharon (2014), Rosenberg, Eugene; DeLong, Edward F.; Lory, Stephen; Stackebrandt, Erko (eds.), "The Family Halobacteriaceae", The Prokaryotes: Other Major Lineages of Bacteria and The Archaea, Berlin, Heidelberg: Springer, pp. 41–121, Bibcode:2014prok.book...41O, doi:10.1007/978-3-642-38954-2_313, ISBN   978-3-642-38954-2 , retrieved 2021-11-17
  3. 1 2 3 4 5 6 Baliga, Nitin S.; Bonneau, Richard; Facciotti, Marc T.; Pan, Min; Glusman, Gustavo; Deutsch, Eric W.; Shannon, Paul; Chiu, Yulun; Weng, Rueyhung Sting; Gan, Rueichi Richie; Hung, Pingliang (2004-11-01). "Genome sequence of Haloarcula marismortui: A halophilic archaeon from the Dead Sea". Genome Research. 14 (11): 2221–2234. doi:10.1101/gr.2700304. ISSN   1088-9051. PMC   525680 . PMID   15520287.
  4. "Haloarcula marismortui". NCBI. Retrieved 2021-11-22.
  5. Syutkin, Alexey S.; Pyatibratov, Mikhail G.; Galzitskaya, Oxana V.; Rodríguez-Valera, Francisco; Fedorov, Oleg V. (2014-03-01). "Haloarcula marismortui archaellin genes as ecoparalogs". Extremophiles. 18 (2): 341–349. doi:10.1007/s00792-013-0619-4. ISSN   1433-4909. PMID   24368632. S2CID   254084670.