Hanseniaspora gamundiae

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Hanseniaspora gamundiae
Scientific classification OOjs UI icon edit-ltr.svg
Domain: Eukaryota
Kingdom: Fungi
Division: Ascomycota
Class: Saccharomycetes
Order: Saccharomycetales
Family: Saccharomycodaceae
Genus: Hanseniaspora
Species:
H. gamundiae
Binomial name
Hanseniaspora gamundiae
Libkind, Čadež & Hittinger 2019 [1]

Hanseniaspora gamundiae is a species of yeast in the family Saccharomycodaceae. It has been isolated from the fruiting bodies of Cyttaria hariotii mushrooms in Patagonia and is likely responsible for the early stages of fermentation of an alcoholic chicha produced from the mushrooms.

Contents

Taxonomy

Samples of H. gamundiae were first isolated from samples taken from the stromata of edible Cyttaria hariotii mushrooms growing on southern beech trees in Patagonia in Spring 2007. [2] Genetic testing revealed that the yeast was a previously undescribed species and it was given the specific epithet "gamundiae" in honor of Dr. Irma Gamundi in Argentina in recognition for her taxonomic work with fungi and particularly with the Cyttaria genus. [2]

Genetic sequencing shows that the species is closely related to Hanseniaspora taiwanica and Hanseniaspora occidentalis . [2]

Description

Microscopic examination of the yeast cells in YM liquid medium after 48 hours at 25°C reveals cells that are 4.3 to 15.7 μm by 2.4 to 4.7 μm in size, apiculate, ovoid to elongate, appearing singly or in pairs. Reproduction is by budding, which occurs at both poles of the cell. In broth culture, sediment is present, and after one month a very thin ring is formed. [2]

Colonies that are grown on malt agar for one month at 25°C appear cream-colored, butyrous, glossy, and smooth. Growth is flat to slightly raised at the center, with an entire to slightly undulating margin. The yeast forms poorly-developed pseudohyphae on cornmeal agar. The yeast has been observed to form one to two spherical and warty ascospores when grown for at least two weeks on 5% Difco malt extract agar. [2]

The yeast can ferment glucose and can weakly ferment sucrose, but not galactose, cellobiose, maltose, or lactose. The yeast can assimilate glucose, sucrose, cellobiose, arbutin, and salicin. It has a positive growth rate at 30°C, but there is no growth at 35°C. It can grow on agar media containing 10% sodium chloride but absent on media with 16% sodium chloride. Growth on agar with 50% glucose-yeast extract agar, 1% acetic acid, and 0.01% cycloheximide is absent. [2]

Ecology

The species was collected during a study of yeasts that were present on species of Cyttaria mushrooms. Mature mushrooms are composed of up to 10.2% of the fructose, glucose, and sucrose, which resembles the composition of grape juice, a habitat that is well-known to contain Hanseniaspora species. The Mapuche people of Patagonia consumed the Cyttaria in several ways, including in the production of the alcoholic beverage chicha. [3] They used the mushrooms by collecting the mature stomata and either squeezing them to obtain the juice, or by leaving the stromata in cooled boiled water for a few days, after which it spontaneously ferments. [2] Due to the composition of yeasts found in Cyttaria , it is believed that Hanseniaspora gamundiae plays a significant role in the early stages of the fermentation of the beverage, followed by naturally-occurring Saccharomyces cerevisiae , Saccharomyces uvarum , or Saccharomyces eubayanus species. [2]

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Hanseniaspora opuntiae is a species of yeast in the family Saccharomycetaceae. It has been isolated from locations worldwide, on grape berries and on prickly pear cacti.

Hanseniaspora meyeri is a species of yeast in the family Saccharomycetaceae. Samples of the species have been obtained worldwide from flowers, fruit flies, stem rot, and spoiled grape punch.

Hanseniaspora occidentalis is a species of yeast in the family Saccharomycetaceae. In its anamorph form, it was called Kloeckera javanica. It has been isolated in the wild from soil samples and vineyards. Samples of a variant have been isolated from orange juice and rotten oranges. It has demonstrated potential as an organism to reduce malic acid in wine production.

Hanseniaspora osmophila is a species of yeast in the family Saccharomycetaceae. It is found in soil and among the bark, leaves, and fruits of plants, as well as fermented foods and beverages made from fruit.

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Zygosaccharomyces rouxii is a species of yeast in the genus Zygosaccharomyces. Initially described as Saccharomyces rouxii by Boutroux in 1883, it was then moved to the genus Zygosaccharomyces in the work of Barnett et al. It is remarkably tolerant of high concentrations of sugar or salt, making it a spoilage agent of otherwise stable foods, but also present in fermentation of products such as soy sauce or balsamic vinegar.

References

  1. Kirk, Paul (Feb 2020). Kirk, Paul (ed.). "Hanseniaspora gamundiae Libkind, Čadež & Hittinger". Species Fungorum for CoL+. Catalogue of Life. doi:10.48580/dfp3-4hj.
  2. 1 2 3 4 5 6 7 8 Čadež, Neža; Bellora, Nicolas; Ulloa, Ricardo; Hittinger, Chris Todd; Libkind, Diego (2019). "Genomic content of a novel yeast species Hanseniaspora gamundiae sp. nov. from fungal stromata (Cyttaria) associated with a unique fermented beverage in Andean Patagonia, Argentina". PLOS ONE. 14 (1): e0210792. Bibcode:2019PLoSO..1410792C. doi: 10.1371/journal.pone.0210792 . PMC   6353571 . PMID   30699175.
  3. Schmeda-Hirschmann, Guillermo; Razmilic, Ivan; Reyes, Sergio; Gutierrez, Margarita I.; Loyola, Jose I. (1999). "Biological Activity and Food Analysis of Cyttaria Spp. (Discomycetes)". Economic Botany. 53 (1): 30–40. doi:10.1007/BF02860789. S2CID   39320660.