INCENP

Last updated
INCENP
Available structures
PDB Ortholog search: PDBe RCSB
Identifiers
Aliases INCENP , inner centromere protein
External IDs OMIM: 604411; MGI: 1313288; HomoloGene: 9624; GeneCards: INCENP; OMA:INCENP - orthologs
Orthologs
SpeciesHumanMouse
Entrez
Ensembl
UniProt
RefSeq (mRNA)

NM_001040694
NM_020238

NM_016692
NM_001369356

RefSeq (protein)

NP_001035784
NP_064623

NP_057901

Location (UCSC) Chr 11: 62.12 – 62.15 Mb Chr 19: 9.85 – 9.88 Mb
PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse
Chromosome passenger complex (CPC) protein INCENP N terminal
Identifiers
SymbolINCENP_N
Pfam PF12178
InterPro IPR022006
Available protein structures:
Pfam   structures / ECOD  
PDB RCSB PDB; PDBe; PDBj
PDBsum structure summary
Inner centromere protein, ARK binding region
Identifiers
SymbolINCENP_ARK-bind
Pfam PF03941
InterPro IPR005635
Available protein structures:
Pfam   structures / ECOD  
PDB RCSB PDB; PDBe; PDBj
PDBsum structure summary

Inner centromere protein is a protein that in humans is encoded by the INCENP gene. [5] [6] [7] It is a regulatory protein in the chromosome passenger complex (CPC). [8] It is involved in regulation of the catalytic proteins Aurora B and Aurora C. [9] [10] [11] It acts in association with two other proteins - Survivin and Borealin. These proteins form a tight three-helical bundle. The N-terminal domain of INCENP is the domain involved in formation of this three-helical bundle while its C-terminal domain is responsible for the interaction with Aurora B. [12] [11]

Contents

In mammalian cells, two broad groups of centromere-interacting proteins have been described: constitutively binding centromere proteins and 'passenger' (or transiently interacting) proteins. [13] The constitutive proteins include CENPA (centromere protein A), CENPB, CENPC1, and CENPD.

The term 'passenger proteins' encompasses a broad collection of proteins that localize to the centromere during specific stages of the cell cycle. [14] These include CENPE; MCAK; KID; cytoplasmic dynein (e.g., DYNC1H1); CliPs (e.g. CLIP1); and CENPF/mitosin (CENPF). The inner centromere proteins (INCENPs), [5] the initial members of the passenger protein group, display a broad localization along chromosomes in the early stages of mitosis but gradually become concentrated at centromeres as the cell cycle progresses into mid-metaphase. During telophase, the proteins are located within the midbody in the intercellular bridge, where they are discarded after cytokinesis. [7] [15]

Interactions

INCENP has been shown to interact with H2AFZ, [16] Survivin [17] and CDCA8. [18] The ARK binding region has been found to be necessary and sufficient for binding to aurora-related kinase. This interaction has been implicated in the coordination of chromosome segregation with cell division in yeast. [19]

References

  1. 1 2 3 GRCh38: Ensembl release 89: ENSG00000149503 Ensembl, May 2017
  2. 1 2 3 GRCm38: Ensembl release 89: ENSMUSG00000024660 Ensembl, May 2017
  3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  5. 1 2 Earnshaw WC, Cooke CA (Sep 1991). "Analysis of the distribution of the INCENPs throughout mitosis reveals the existence of a pathway of structural changes in the chromosomes during metaphase and early events in cleavage furrow formation". J Cell Sci. 98 (4): 443–61. doi:10.1242/jcs.98.4.443. PMID   1860899.
  6. Adams RR, Eckley DM, Vagnarelli P, Wheatley SP, Gerloff DL, Mackay AM, Svingen PA, Kaufmann SH, Earnshaw WC (Jul 2001). "Human INCENP colocalizes with the Aurora-B/AIRK2 kinase on chromosomes and is overexpressed in tumour cells". Chromosoma. 110 (2): 65–74. doi:10.1007/s004120100130. PMID   11453556. S2CID   13778759.
  7. 1 2 "Entrez Gene: INCENP inner centromere protein antigens 135/155kDa".
  8. Dietz, Anne; Subedi, Prabal; Azimzadeh, Omid; Duchrow, Lukas; Kaestle, Felix; Paetzold, Juliane; Katharina Payer, Sarah; Hornhardt, Sabine; von Toerne, Christine; Hauck, Stefanie M.; Kempkes, Bettina; Kuklik-Roos, Cornelia; Brandes, Danielle; Borkhardt, Arndt; Moertl, Simone; Gomolka, Maria (2024). "The Chromosome Passenger Complex (CPC) Components and Its Associated Pathways Are Promising Candidates to Differentiate Between Normosensitive and Radiosensitive ATM-Mutated Cells". Biomarker Insights. 19. doi:10.1177/11772719241274017.
  9. Sasai, Kaori; Katayama, Hiroshi; Hawke, David H; Sen, Subrata (2016-01-01). "Aurora-C Interactions with Survivin and INCENP Reveal Shared and Distinct Features Compared with Aurora-B Chromosome Passenger Protein Complex". PLOS ONE. 11 (6): e0157305. Bibcode:2016PLoSO..1157305S. doi: 10.1371/journal.pone.0157305 . ISSN   1932-6203. PMC   4917241 . PMID   27332895.
  10. Li, Xiangyu; Sakashita, Gyosuke; Matsuzaki, Hideki; Sugimoto, Kenji; Kimura, Keiji; Hanaoka, Fumio; Taniguchi, Hisaaki; Furukawa, Koichi; Urano, Takeshi (2004-11-01). "Direct association with inner centromere protein (INCENP) activates the novel chromosomal passenger protein, Aurora-C". The Journal of Biological Chemistry. 279 (45): 47201–47211. doi: 10.1074/jbc.m403029200 . ISSN   1083-351X. PMID   15316025.
  11. 1 2 Honda, Reiko; Körner, Roman; Nigg, Erich A (2003-08-01). "Exploring the functional interactions between Aurora B, INCENP, and survivin in mitosis". Molecular Biology of the Cell. 14 (8): 3325–3341. doi:10.1091/mbc.e02-11-0769. ISSN   1939-4586. PMC   181570 . PMID   12925766.
  12. Jeyaprakash, A. Arockia; Klein, Ulf R.; Lindner, Doris; Ebert, Judith; Nigg, Erich A.; Conti, Elena (2007-10-19). "Structure of a Survivin–Borealin–INCENP Core Complex Reveals How Chromosomal Passengers Travel Together". Cell. 131 (2): 271–285. doi: 10.1016/j.cell.2007.07.045 . ISSN   0092-8674. PMID   17956729. S2CID   15199822.
  13. Choo, K. H. Andy (1997). The centromere . Oxford [Oxfordshire]: Oxford University Press. ISBN   978-0-19-857780-5.
  14. Earnshaw WC, Mackay AM (September 1994). "Role of nonhistone proteins in the chromosomal events of mitosis". FASEB J. 8 (12): 947–56. doi: 10.1096/fasebj.8.12.8088460 . PMID   8088460. S2CID   14062246.
  15. Cutts SM, Fowler KJ, Kile BT, Hii LL, O'Dowd RA, Hudson DF, Saffery R, Kalitsis P, Earle E, Choo KH (July 1999). "Defective chromosome segregation, microtubule bundling and nuclear bridging in inner centromere protein gene (Incenp)-disrupted mice". Hum. Mol. Genet. 8 (7): 1145–55. doi: 10.1093/hmg/8.7.1145 . PMID   10369859.
  16. Rangasamy D, Berven L, Ridgway P, Tremethick DJ (April 2003). "Pericentric heterochromatin becomes enriched with H2A.Z during early mammalian development". EMBO J. 22 (7): 1599–607. doi:10.1093/emboj/cdg160. PMC   152904 . PMID   12660166.
  17. Wheatley SP, Carvalho A, Vagnarelli P, Earnshaw WC (June 2001). "INCENP is required for proper targeting of Survivin to the centromeres and the anaphase spindle during mitosis". Curr. Biol. 11 (11): 886–90. Bibcode:2001CBio...11..886W. doi: 10.1016/S0960-9822(01)00238-X . PMID   11516652.
  18. Gassmann R, Carvalho A, Henzing AJ, Ruchaud S, Hudson DF, Honda R, Nigg EA, Gerloff DL, Earnshaw WC (July 2004). "Borealin: a novel chromosomal passenger required for stability of the bipolar mitotic spindle". J. Cell Biol. 166 (2): 179–91. doi:10.1083/jcb.200404001. PMC   2172304 . PMID   15249581.
  19. Leverson JD, Huang HK, Forsburg SL, Hunter T (April 2002). "The Schizosaccharomyces pombe aurora-related kinase Ark1 interacts with the inner centromere protein Pic1 and mediates chromosome segregation and cytokinesis". Mol. Biol. Cell. 13 (4): 1132–43. doi:10.1091/mbc.01-07-0330. PMC   102257 . PMID   11950927.

Further reading

This article incorporates text from the public domain Pfam and InterPro: IPR005635
This article incorporates text from the public domain Pfam and InterPro: IPR022006