Komagataella mondaviorum

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Komagataella mondaviorum
Scientific classification OOjs UI icon edit-ltr.svg
Domain: Eukaryota
Kingdom: Fungi
Division: Ascomycota
Class: Saccharomycetes
Order: Saccharomycetales
Family: Phaffomycetaceae
Genus: Komagataella
Species:
K. mondaviorum
Binomial name
Komagataella mondaviorum
Naumov, 2018

Komagataella mondaviorum is a species of ascomycete fungi existing as a yeast.

Contents

Discovery

Komagataella mondaviorum was identified as a unique species in 2018 from strains collected throughout the 1950s and 1970s. The strains were previously believed to be Komagataella pastoris . [1] K. mondaviorum was declared a distinct species due to differences in the genome found during DNA sequencing. [1] It was named after Robert and Margrit Mondavi for their contributions to the California wine industry. [1]

Growth and morphology

Komagataella mondaviorum, when grown on malt extract agar, has been described as spherical or ovoid, with lengths ranging between 2-7 μm. [1] Cells are observed to grow singly or in pairs. Colonies are white and smooth. Hat-shaped ascospores have been observed. [1]

Glucose is fermented by K. mondaviorum; fermentation of other common sugars has not been observed. [1] No growth occurs at or above 35°C. [1] [2]

Phylogeny

Within Komagataella , K. mondaviorum is considered to be most closely related to K. populi and K. pseudopastoris . [1] [2] [3]

Ecology and distribution

Komagataella mondaviorum has been isolated from various tree species, including cottonwood ( Populus deltoides ), black cottonwood ( Populus trichocarpa ), black oak ( Quercus kelloggii ), willow ( Salix sp.), and hackberry ( Celtis sp.). [1] [3] It has been discovered in the United States (California, Washington, and Alaska) as well as Canada (British Columbia). [1] [3]

Utility in industrial protein synthesis

Komagataella mondaviorum has been proposed as a promising candidate for protein synthesis at an industrial scale, with the potential to synthesize products more efficiently than commonly used Komagataella species such as K. phaffii . [4]

References

  1. 1 2 3 4 5 6 7 8 9 10 Naumov, Gennadi I.; Naumova, Elena S.; Boundy-Mills, Kyria L. (2018-07-01). "Description of Komagataella mondaviorum sp. nov., a new sibling species of Komagataella (Pichia) pastoris". Antonie van Leeuwenhoek. 111 (7): 1197–1207. doi:10.1007/s10482-018-1028-6. ISSN   1572-9699.
  2. 1 2 Heistinger, Lina; Dohm, Juliane C.; Paes, Barbara G.; Koizar, Daniel; Troyer, Christina; Ata, Özge; Steininger-Mairinger, Teresa; Mattanovich, Diethard (2022-04-25). "Genotypic and phenotypic diversity among Komagataella species reveals a hidden pathway for xylose utilization". Microbial Cell Factories. 21 (1): 70. doi: 10.1186/s12934-022-01796-3 . ISSN   1475-2859. PMC   9036795 . PMID   35468837.
  3. 1 2 3 Naumova, E. S.; Boundy-Mills, K. L.; Naumov, G. I. (2020-05-01). "Phylogenetics and Biogeography of Biotechnologically Important Methylotrophic Yeasts Komagataella". Microbiology. 89 (3): 294–300. doi:10.1134/S002626172003011X. ISSN   1608-3237.
  4. Gordeeva, T. L.; Borschevskaya, L. N.; Feday, T. D.; Tkachenko, A. A.; Sineoky, S. P. (2022-12-01). "The Expression Potential of Novel Komagataella Strains". Applied Biochemistry and Microbiology. 58 (8): 916–922. doi:10.1134/S0003683822080038. ISSN   1608-3024.