List of restriction enzyme cutting sites: Bsa–Bso

Main articles: List of restriction enzyme cutting sites and Restriction enzyme

Legend of nucleobases
Code	Nucleotide represented
A	Adenine (A)
C	Cytosine (C)
G	Guanine (G)
T	Thymine (T)
N	A, C, G or T
M	A or C
R	A or G
W	A or T
Y	C or T
S	C or G
K	G or T
H	A, C or T
B	C, G or T
V	A, C or G
D	A, G or T

This article contains a list of the most studied restriction enzymes whose names start with Bsa to Bso inclusive. It contains approximately 90 enzymes. The following information is given:

• Enzyme: Accepted name of the molecule, according to the internationally adopted nomenclature ^{[1] [2]} , and bibliographical references. (Further reading: see the section "Nomenclature" in the article "Restriction enzyme".)
• PDB code: Code used to identify the structure of a protein in the PDB database of protein structures. The 3D atomic structure of a protein provides highly valuable information to understand the intimate details of its mechanism of action ^{[3] [4]} .
• Source: Organism that naturally produces the enzyme.
• Recognition sequence : Sequence of DNA recognized by the enzyme and to which it specifically binds.
• Cut: Cutting site and DNA products of the cut. The recognition sequence and the cutting site usually match, but sometimes the cutting site can be dozens of nucleotides away from the recognition site ^{[5] [6]} .
• Isoschizomers and neoschizomers: An isoschizomer is an enzyme that recognizes the same sequence as another. A neoschizomer is a special type of isoschizomer that recognizes the same sequence as another, but cuts in a different manner. A maximum number of 8-10 most common isoschizomers are indicated for every enzyme but there may be many more. Neoschizomers are shown in bold and green color font (e.g.: BamHI). When "None on date" is indicated, that means that there were no registered isoschizomers in the databases on that date with a clearly defined cutting site. Isoschizomers indicated in white font and grey background correspond to enzymes not listed in the current lists:

as in this not listed enzyme:

EcoR70I

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Restriction enzymes

Bsa - Bso

Enzyme	PDB code	Source	Recognition sequence	Cut	Isoschizomers
BsaI		Bacillus stearothermophilus 20241	5' GGTCTC 3' CCAGAG	5' ---GGTCTCN   NNNN--- 3' 3' ---CCAGAGNNNNN   --- 5'	Bso31I, BspTNI, Eco31I
Bsa29I		Bacillus sp. 29	5' ATCGAT 3' TAGCTA	5' ---AT   CGAT--- 3' 3' ---TAGC   TA--- 5'	AagI, BanIII, BavCI, BseCI, BspDI, Bsu15I, BsuTUI, ClaI, BbvAII
BsaAI		Bacillus stearothermophilus G668	5' YACGTR 3' RTGCAY	5' ---YAC   GTR--- 3' 3' ---RTG   CAY--- 5'
BsaBI		Bacillus stearothermophilus B674	5' GATN4ATC 3' CTAN4TAG	5' ---GATNN   NNATC--- 3' 3' ---CTANN   NNTAG--- 5'
BsaHI [7]		Bacillus stearothermophilus CPW11	5' GRCGYC 3' CYGCRG	5' ---GR   CGYC--- 3' 3' ---CYGC   RG--- 5'	AcyI, AhaII, AosII, AstWI, AsuIII, BbiII, BstACI, HgiI, PamII
BsaJI		Bacillus stearothermophilus J695	5' CCNNGG 3' GGNNCC	5' ---C   CNNGG--- 3' 3' ---GGNNC   C--- 5'
BsaMI		Bacillus stearothermophilus M293	5' GAATGC 3' CTTACG	5' ---GAATGCN   --- 3' 3' ---CTTAC   GN--- 5'	Asp26HI, Asp35HI, Asp36HI, Asp50HI, BsaMI, BsmI, Mva1269I
BsaOI		Bacillus stearothermophilus O-122	5' CGRYCG 3' GCYRGC	5' ---CGRY   CG--- 3' 3' ---GC   YRGC--- 5'
BsaWI		Bacillus stearothermophilus W1718	5' WCCGGW 3' WGGCCW	5' ---W   CCGGW--- 3' 3' ---WGGCC   W--- 5'
BsaXI [8]		Bacillus stearothermophilus 25B	5' ACN5CTCC 3' TGN5GAGG	5' ---ACN5CTCCN6NNNN   --- 3' 3' ---TGN5GAGGN6N   NNN--- 5'	BsmCI, BsmDI, BsmXI
BscI		Bacillus sp.	5' ATCGAT 3' TAGCTA	5' ---AT   CGAT--- 3' 3' ---TAGC   TA--- 5'	BanIII, BavCI, Bci29I, BdiI, Bli41I, BliAI, LplI, PgaI, SpmI
Bsc4I		Bacillus schlegelii 4	5' CCN7GG 3' GGN7CC	5' ---CCNNNNN   NNGG--- 3' 3' ---GGNN   NNNNNCC--- 5'
Bsc91I		Bacillus sp.	5' GAAGAC 3' CTTCTG	5' ---GAAGACNN   NNNN--- 3' 3' ---CTTCTGNNNNNN   --- 5'
Bsc107I		Bacillus schlegelii 107	5' CCN7GG 3' GGN7CC	5' ---CCNNNNN   NNGG--- 3' 3' ---GGNN   NNNNNCC--- 5'
BscAI		Bacillus schlegelii S3	5' GCATC 3' CGTAG	5' ---GCATCNNNN   NN--- 3' 3' ---CGTAGNNNNNN   --- 5'
BscBI		Bacillus sp. A11	5' GGNNCC 3' CCNNGG	5' ---GGN   NCC--- 3' 3' ---CCN   NGG--- 5'	AspNI,  BmiI,  BspLI, NlaIV, PspN4I
BscCI		Bacillus sp. 2G	5' GAATGC 3' CTTACG	5' ---GAATGCN   --- 3' 3' ---CTTAC   GN--- 5'	Asp26HI, Asp27HI, Asp35HI, Asp36HI, BsaMI, BscCI, PctI
BscFI		Bacillus sp. JY391	5' GATC 3' CTAG	5' ---   GATC--- 3' 3' ---CTAG   --- 5'	Bfi57I, Bsp143I, BspJI, BtkII, CviAI, Kzo9I, NlaII, SsiBI
Bse1I		Bacillus stearothermophilus 1	5' ACTGG 3' TGACC	5' ---ACTGGN   --- 3' 3' ---TGAC   CN--- 5'
Bse8I		Bacillus sp. 8	5' GATN4ATC 3' CTAN4TAG	5' ---GATNN   NNATC--- 3' 3' ---CTANN   NNTAG--- 5'
Bse15I		Bacillus sp. 15	5' CYCGRG 3' GRGCYC	5' ---C   YCGRG--- 3' 3' ---GRGCY   C--- 5'	Ama87I, AvaI, BcoI, BsoBI, BstSI, Eco88I, NspSAI, OfoI
Bse16I		Bacillus sp. 16	5' CCWGG 3' GGWCC	5' ---CC   WGG--- 3' 3' ---GGW   CC--- 5'	AeuI, BseBI, BsiLI, BstNI, BstM6I, BthDI, BthEI, EcoRII, MvaI
Bse17I		Bacillus sp. 17	5' CCWGG 3' GGWCC	5' ---CC   WGG--- 3' 3' ---GGW   CC--- 5'	AeuI, BseBI, Bse16I, Bst2I, BstNI, Bst38I, Bst100I, Psp6I, PspGI
Bse21I		Bacillus sp. 21	5' CCTNAGG 3' GGANTCC	5' ---CC   TNAGG--- 3' 3' ---GGANT   CC--- 5'	AxyI, BliHKI, BspR7I, Bsu36I, Eco81I, MstII, OxaNI, SshAI
Bse24I		Bacillus sp. 24	5' CCWGG 3' GGWCC	5' ---CC   WGG--- 3' 3' ---GGW   CC--- 5'	AeuI, BseBI, Bse16I, Bst2I, BstNI, Bst38I, Bst100I, Psp6I, PspGI
Bse64I		Bacillus sp. 64	5' GGTNACC 3' CCANTGG	5' ---G   GTNACC--- 3' 3' ---CCANTG   G--- 5'	AspAI, Bse64I, BseT9I, BstPI, EcaI, Eci125I, EcoO65I, NspSAII
Bse118I [9]		Bacillus sp. 118	5' RCCGGY 3' YGGCCR	5' ---R   CCGGY--- 3' 3' ---YGGCC   R--- 5'
Bse634I	1KNV	Bacillus sp. 634	5' RCCGGY 3' YGGCCR	5' ---R   CCGGY--- 3' 3' ---YGGCC   R--- 5'
BseAI [10]		Bacillus sphaericus	5' TCCGGA 3' AGGCCT	5' ---T   CCGGA--- 3' 3' ---AGGCC   T--- 5'	AccIII, Aor13HI, BlfI, BsiMI, Bsp13I, BspMII, CauB3I, Kpn2I
BseBI [11]		Bacillus stearothermophilus	5' CCWGG 3' GGWCC	5' ---CC   WGG--- 3' 3' ---GGW   CC--- 5'	AjnI, AorI, Bse17I, Bst1I, BstOI, Bst2UI, EcoRII, MvaI, SleI, ZanI
BseCI [12] [13] [14]		Bacillus sp.	5' ATCGAT 3' TAGCTA	5' ---AT   CGAT--- 3' 3' ---TAGC   TA--- 5'	AagI, BanIII, BavCI, Bsa29I, BspDI, Bsu15I, BsuTUI, ClaI, LcaI
BseDI		Bacillus stearothermophilus RFL1434	5' CCNNGG 3' GGNNCC	5' ---C   CNNGG--- 3' 3' ---GGNNC   C--- 5'
Bse3DI		Bacillus stearothermophilus 3D	5' GCAATG 3' CGTTAC	5' ---GCAATGNN   --- 3' 3' ---CGTTAC   NN--- 5'
BseGI		Bacillus stearothermophilus Vs 34-031	5' GGATG 3' CCTAC	5' ---GGATGNN   --- 3' 3' ---CCTAC   NN--- 5'
BseJI		Bacillus stearothermophilus Tsp5	5' GATN4ATC 3' CTAN4TAG	5' ---GATNN   NNATC--- 3' 3' ---CTANN   NNTAG--- 5'
BseKI		Bacillus stearothermophilus Ra3-212	5' GCAGC 3' CGTCG	5' ---GCAGCN7N   NNNN--- 3' 3' ---CGTCGN7NNNNN   --- 5'	AlwXI, BbvI, BseXI, Bsp423I, Bst12I, Bst71I, BstV1I
BseLI		Bacillus stearothermophilus LK3-551	5' CCN7GG 3' GGN7CC	5' ---CCNNNNN   NNGG--- 3' 3' ---GGNN   NNNNNCC--- 5'
BseMI		Bacillus stearothermophilus Isl 15-111	5' GCAATG 3' CGTTAC	5' ---GCAATGNN   --- 3' 3' ---CGTTAC   NN--- 5'
BseMII [15]		Bacillus stearothermophilus Isl 15-111	5' CTCAG 3' GAGTC	5' ---CTCAGN7NNN   --- 3' 3' ---GAGTCN7N   NN--- 5'
BseNI		Bacillus sp. N	5' ACTGG 3' TGACC	5' ---ACTGGN   --- 3' 3' ---TGAC   CN--- 5'
BsePI		Bacillus stearothermophilus P6	5' GCGCGC 3' CGCGCG	5' ---G   CGCGC--- 3' 3' ---CGCGC   G--- 5'
BseQI		Bacillus sp. Q	5' GGCC 3' CCGG	5' ---GG   CC--- 3' 3' ---CC   GG--- 5'
BseRI [16]		Bacillus sp. R	5' GAGGAG 3' CTCCTC	5' ---GAGGAGN7NNN   --- 3' 3' ---CTCCTCN7N   NN--- 5'
BseSI [17]		Bacillus stearothermophilus Jo-553	5' GKGCMC 3' CMCGKG	5' ---GKGCM   C--- 3' 3' ---C   MCGKG--- 5'
BseT9I		Bacillus sp. T9	5' GGTNACC 3' CCANTGG	5' ---G   GTNACC--- 3' 3' ---CCANTG   G--- 5'	AcrII, Bse64I, BstEII, BstT9I, EcaI, Eci125I, EcoO65I, NspSAII, PspEI
BseT10I		Bacillus sp. T10	5' GGTNACC 3' CCANTGG	5' ---G   GTNACC--- 3' 3' ---CCANTG   G--- 5'	AcrII, AspAI, BstEII, BstT10I, BstPI, EcoO128I, EcoO65I, PspEI
BseXI		Bacillus stearothermophilus Ra3-212	5' GCAGC 3' CGTCG	5' ---GCAGCN7N   NNNN--- 3' 3' ---CGTCGN7NNNNN   --- 5'	AlwXI, BbvI, BseKI, Bsp423I, Bst12I, Bst71I, BstV1I
BseX3I		Bacillus stearothermophilus X3	5' CGGCCG 3' GCCGGC	5' ---C   GGCCG--- 3' 3' ---GCCGG   C--- 5'	AaaI, BstZI, EagI, EclXI, Eco52I, SenPT16I, XmaIII
BseYI		Bacillus sp. 2521	5' CCCAGC 3' GGGTCG	5' ---C   CCAGC--- 3' 3' ---GGGTC   G--- 5'
BseZI		Bacillus sp. Z	5' CTCTTC 3' GAGAAG	5' ---CTCTTCN   NNN--- 3' 3' ---GAGAAGNNNN   --- 5'
BsgI		Bacillus sphaericus B922	5' GTGCAG 3' CACGTC	5' ---GTGCAGN12NNNN   --- 3' 3' ---CACGTCN12NN   NN--- 5'
BshI		Bacillus sphaericus	5' GGCC 3' CCGG	5' ---GG   CC--- 3' 3' ---CC   GG--- 5'
Bsh45I		Bacillus sphaericus 45	5' GWGCWC 3' CWCGWG	5' ---GWGCW   C--- 3' 3' ---C   WCGWG--- 5'	Alw21I, AspHI, Bbv12I, BsiHKAI, HgiAI,  HpyF46II,  MspV281I
Bsh1236I		Bacillus sphaericus RFL1236	5' CGCG 3' GCGC	5' ---CG   CG--- 3' 3' ---GC   GC--- 5'	AccII, BceBI, BspFNI, Bsp50I, Csp68KVI, FauBII, MvnI, SelI
Bsh1285I		Bacillus sphaericus RFL1285	5' CGRYCG 3' GCYRGC	5' ---CGRY   CG--- 3' 3' ---GC   YRGC--- 5'
Bsh1365I		Bacillus sphaericus RFL1365	5' GATN4ATC 3' CTAN4TAG	5' ---GATNN   NNATC--- 3' 3' ---CTANN   NNTAG--- 5'
BshFI [18]		Bacillus sphaericus	5' GGCC 3' CCGG	5' ---GG   CC--- 3' 3' ---CC   GG--- 5'
BshGI		Bacillus sphaericus	5' CCWGG 3' GGWCC	5' ---CC   WGG--- 3' 3' ---GGW   CC--- 5'	AeuI, BseBI, Bse16I, Bst2I, BstNI, Bst38I, Bst100I, PspGI, SspAI
BshKI		Bacillus sphaericus	5' GGNCC 3' CCNGG	5' ---G   GNCC--- 3' 3' ---CCNG   G--- 5'	AspS9I, Bac36I, BavAII, Bsp1894I, BspBII, CcuI, MaeK81II, Pde12I
BshNI		Bacillus sphaericus TK-45	5' GGYRCC 3' CCRYGG	5' ---G   GYRCC--- 3' 3' ---CCRYG   G--- 5'	BanI, BbvBI, BspT107I, Eco64I, HgiCI, HgiHI, MspB4I, PfaAI
BshTI		Bacillus sphaericus Jo22-024	5' ACCGGT 3' TGGCCA	5' ---A   CCGGT--- 3' 3' ---TGGCC   A--- 5'	AgeI, AsiAI,  AsiGI,  CsiAI CspAI, PinAI
BsiI [19] [20]		Bacillus sphaericus	5' CACGAG 3' GTGCTC	5' ---C   ACGAG--- 3' 3' ---GTGCT   C--- 5'
BsiBI [21]		Bacillus sp.	5' GATN4ATC 3' CTAN4TAG	5' ---GATNN   NNATC--- 3' 3' ---CTANN   NNTAG--- 5'
BsiCI		Bacillus sp.	5' TTCGAA 3' AAGCTT	5' ---TT   CGAA--- 3' 3' ---AAGC   TT--- 5'	AcpI, AsuII, Bpu14I, BspT104I, Csp45I, FspII, NspV, PpaAI, SfuI
BsiEI [22]		Bacillus sp.	5' CGRYCG 3' GCYRGC	5' ---CGRY   CG--- 3' 3' ---GC   YRGC--- 5'
BsiHKAI [23]		Bacillus stearothermophilus	5' GWGCWC 3' CWCGWG	5' ---GWGCW   C--- 3' 3' ---C   WCGWG--- 5'	Alw21I, AspHI, Bbv12I, Bsh45I,  Bsm6I,  HgiAI, MspV281I
BsiHKCI		Bacillus sp. HKC	5' CYCGRG 3' GRGCYC	5' ---C   YCGRG--- 3' 3' ---GRGCY   C--- 5'	AquI, BcoI, Bse15I, BspLU4I, Eco88I, Nli3877I, PlaAI, PunAI
BsiKI [24]		Bacillus sp.	5' GGTNACC 3' CCANTGG	5' ---G   GTNACC--- 3' 3' ---CCANTG   G--- 5'
BsiLI		Bacillus sp.	5' CCWGG 3' GGWCC	5' ---CC   WGG--- 3' 3' ---GGW   CC--- 5'	AglI, BseBI, Bse17I, Bst2I, BstNI, Bst38I, Bst100I, EcoRII, MvaI
BsiMI		Bacillus sp.	5' TCCGGA 3' AGGCCT	5' ---T   CCGGA--- 3' 3' ---AGGCC   T--- 5'	AccIII, BbvAIII, BlfI, Bsp13I, BspMII, Bsu23I, Kpn2I, PinBII
BsiQI		Bacillus sp.	5' TGATCA 3' ACTAGT	5' ---T   GATCA--- 3' 3' ---ACTAG   T--- 5'	AbaI, BclI, BspXII, BstT7I, FbaI, Ksp22I,  ParI
BsiSI [25]		Bacillus sp.	5' CCGG 3' GGCC	5' ---C   CGG--- 3' 3' ---GGC   C--- 5'
BsiWI		Bacillus sp.	5' CGTACG 3' GCATGC	5' ---C   GTACG--- 3' 3' ---GCATG   C--- 5'
BsiXI		Bacillus sp. X	5' ATCGAT 3' TAGCTA	5' ---AT   CGAT--- 3' 3' ---TAGC   TA--- 5'	BscI, BsiXI, Bsp106I, BspDI, Bsu15I, BsuTUI, ClaI, Rme21I
BsiYI [26]		Bacillus sp.	5' CCN7GG 3' GGN7CC	5' ---CCNNNNN   NNGG--- 3' 3' ---GGNN   NNNNNCC--- 5'
BsiZI		Bacillus sp.	5' GGNCC 3' CCNGG	5' ---G   GNCC--- 3' 3' ---CCNG   G--- 5'	AspS9I, Bac36I, BavAII, BshKI, BspBII, CcuI, MaeK81II, Pde12I
BslI		Bacillus sp.	5' CCN7GG 3' GGN7CC	5' ---CCNNNNN   NNGG--- 3' 3' ---GGNN   NNNNNCC--- 5'
BslFI		Bacillus stearothermophilus FI	5' GGGAC 3' CCCTG	5' ---GGGACN8NN   NNNN--- 3' 3' ---CCCTGN8NNNNNN   --- 5'
BsmI		Bacillus stearothermophilus NUB	5' GAATGC 3' CTTACG	5' ---GAATGCN   --- 3' 3' ---CTTAC   GN--- 5'	Asp26HI, Asp36HI, Asp40HI, BmaHI, BscCI, Mva1269I, PctI
BsmAI		Bacillus stearothermophilus A664	5' GTCTC 3' CAGAG	5' ---GTCTCN   NNNN--- 3' 3' ---CAGAGNNNNN   --- 5'	Alw26I,  BcoDI, BscQII,  BsoMAI, BstMAI
BsmBI		Bacillus stearothermophilus B61	5' CGTCTC 3' GCAGAG	5' ---CGTCTCN   NNNN--- 3' 3' ---GCAGAGNNNNN   --- 5'
BsmFI		Bacillus stearothermophilus F	5' GGGAC 3' CCCTG	5' ---GGGACN8NN   NNNN--- 3' 3' ---CCCTGN8NNNNNN   --- 5'
BsmSI		Bacillus stearothermophilus CP114	5' CCWWGG 3' GGWWCC	5' ---C   CWWGG--- 3' 3' ---GGWWC   C--- 5'
Bso31I		Bacillus stearothermophilus E31	5' GGTCTC 3' CCAGAG	5' ---GGTCTCN   NNNN--- 3' 3' ---CCAGAGNNNNN   --- 5'
BsoBI	1DC1	Bacillus stearothermophilus JN2091	5' CYCGRG 3' GRGCYC	5' ---C   YCGRG--- 3' 3' ---GRGCY   C--- 5'	AquI, BcoI, BsiHKCI, BspLU4I, Eco88I, Nli3877I, PlaAI, PunAI
BsoCI		Bacillus stearothermophilus AU891	5' GDGCHC 3' CHCGDG	5' ---GDGCH   C--- 3' 3' ---C   HCGDG--- 5'	AocII, BmyI, Bsp1286I, BspLS2I, MhlI, NspII, SduI
BsoFI		Bacillus stearothermophilus F66	5' GCNGC 3' CGNCG	5' ---GC   NGC--- 3' 3' ---CGN   CG--- 5'
BsoMAI		Bacillus stearothermophilus MA	5' GTCTC 3' CAGAG	5' ---GTCTCN   NNNN--- 3' 3' ---CAGAGNNNNN   --- 5'	Alw26I,  BcoDI, BscQII,  BsmAI, BstMAI

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Notes

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