List of restriction enzyme cutting sites: Bsa–Bso

Last updated

Legend of nucleobases
Code Nucleotide represented
A Adenine (A)
C Cytosine (C)
G Guanine (G)
T Thymine (T)
NA, C, G or T
MA or C
RA or G
WA or T
YC or T
SC or G
KG or T
HA, C or T
BC, G or T
VA, C or G
DA, G or T

This article contains a list of the most studied restriction enzymes whose names start with Bsa to Bso inclusive. It contains approximately 90 enzymes. The following information is given:

Contents

as in this not listed enzyme: EcoR70I 

Whole list navigation

Restriction enzymes

Bsa - Bso

EnzymePDB codeSource Recognition sequence Cut Isoschizomers
BsaI Bacillus stearothermophilus 20241 5' GGTCTC
3' CCAGAG
5' ---GGTCTCN   NNNN--- 3'
3' ---CCAGAGNNNNN   --- 5'
Bso31I, BspTNI, Eco31I
Bsa29I Bacillus sp. 29 5' ATCGAT
3' TAGCTA
5' ---AT   CGAT--- 3'
3' ---TAGC   TA--- 5'
AagI, BanIII, BavCI, BseCI, BspDI, Bsu15I, BsuTUI, ClaI, BbvAII
BsaAI Bacillus stearothermophilus G668 5' YACGTR
3' RTGCAY
5' ---YAC   GTR--- 3'
3' ---RTG   CAY--- 5'
BsaBI Bacillus stearothermophilus B674 5' GATN4ATC
3' CTAN4TAG
5' ---GATNN   NNATC--- 3'
3' ---CTANN   NNTAG--- 5'
BsaHI [7] Bacillus stearothermophilus CPW11 5' GRCGYC
3' CYGCRG
5' ---GR   CGYC--- 3'
3' ---CYGC   RG--- 5'
AcyI, AhaII, AosII, AstWI, AsuIII, BbiII, BstACI, HgiI, PamII
BsaJI Bacillus stearothermophilus J695 5' CCNNGG
3' GGNNCC
5' ---C   CNNGG--- 3'
3' ---GGNNC   C--- 5'
BsaMI Bacillus stearothermophilus M293 5' GAATGC
3' CTTACG
5' ---GAATGCN   --- 3'
3' ---CTTAC   GN--- 5'
Asp26HI, Asp35HI, Asp36HI, Asp50HI, BsaMI, BsmI, Mva1269I
BsaOI Bacillus stearothermophilus O-122 5' CGRYCG
3' GCYRGC
5' ---CGRY   CG--- 3'
3' ---GC   YRGC--- 5'
BsaWI Bacillus stearothermophilus W1718 5' WCCGGW
3' WGGCCW
5' ---W   CCGGW--- 3'
3' ---WGGCC   W--- 5'
BsaXI [8] Bacillus stearothermophilus 25B 5' ACN5CTCC
3' TGN5GAGG
5' ---ACN5CTCCN6NNNN   --- 3'
3' ---TGN5GAGGN6N   NNN--- 5'
 BsmCI, BsmDI, BsmXI 
BscI Bacillus sp. 5' ATCGAT
3' TAGCTA
5' ---AT   CGAT--- 3'
3' ---TAGC   TA--- 5'
BanIII, BavCI, Bci29I, BdiI, Bli41I, BliAI, LplI, PgaI, SpmI
Bsc4I Bacillus schlegelii 4 5' CCN7GG
3' GGN7CC
5' ---CCNNNNN   NNGG--- 3'
3' ---GGNN   NNNNNCC--- 5'
Bsc91I Bacillus sp. 5' GAAGAC
3' CTTCTG
5' ---GAAGACNN   NNNN--- 3'
3' ---CTTCTGNNNNNN   --- 5'
Bsc107I Bacillus schlegelii 107 5' CCN7GG
3' GGN7CC
5' ---CCNNNNN   NNGG--- 3'
3' ---GGNN   NNNNNCC--- 5'
BscAI Bacillus schlegelii S3 5' GCATC
3' CGTAG
5' ---GCATCNNNN   NN--- 3'
3' ---CGTAGNNNNNN   --- 5'
BscBI Bacillus sp. A11 5' GGNNCC
3' CCNNGG
5' ---GGN   NCC--- 3'
3' ---CCN   NGG--- 5'
AspNI,  BmiI, BspLI, NlaIV,
PspN4I
BscCI Bacillus sp. 2G 5' GAATGC
3' CTTACG
5' ---GAATGCN   --- 3'
3' ---CTTAC   GN--- 5'
Asp26HI, Asp27HI, Asp35HI, Asp36HI, BsaMI, BscCI, PctI
BscFI Bacillus sp. JY391 5' GATC
3' CTAG
5' ---   GATC--- 3'
3' ---CTAG   --- 5'
Bfi57I, Bsp143I, BspJI, BtkII, CviAI, Kzo9I, NlaII, SsiBI
Bse1I Bacillus stearothermophilus 1 5' ACTGG
3' TGACC
5' ---ACTGGN   --- 3'
3' ---TGAC   CN--- 5'
Bse8I Bacillus sp. 8 5' GATN4ATC
3' CTAN4TAG
5' ---GATNN   NNATC--- 3'
3' ---CTANN   NNTAG--- 5'
Bse15I Bacillus sp. 15 5' CYCGRG
3' GRGCYC
5' ---C   YCGRG--- 3'
3' ---GRGCY   C--- 5'
Ama87I, AvaI, BcoI, BsoBI, BstSI, Eco88I, NspSAI, OfoI
Bse16I Bacillus sp. 16 5' CCWGG
3' GGWCC
5' ---CC   WGG--- 3'
3' ---GGW   CC--- 5'
AeuI, BseBI, BsiLI, BstNI, BstM6I, BthDI, BthEI, EcoRII, MvaI
Bse17I Bacillus sp. 17 5' CCWGG
3' GGWCC
5' ---CC   WGG--- 3'
3' ---GGW   CC--- 5'
AeuI, BseBI, Bse16I, Bst2I, BstNI, Bst38I, Bst100I, Psp6I, PspGI
Bse21I Bacillus sp. 21 5' CCTNAGG
3' GGANTCC
5' ---CC   TNAGG--- 3'
3' ---GGANT   CC--- 5'
AxyI, BliHKI, BspR7I, Bsu36I, Eco81I, MstII, OxaNI, SshAI
Bse24I Bacillus sp. 24 5' CCWGG
3' GGWCC
5' ---CC   WGG--- 3'
3' ---GGW   CC--- 5'
AeuI, BseBI, Bse16I, Bst2I, BstNI, Bst38I, Bst100I, Psp6I, PspGI
Bse64I Bacillus sp. 64 5' GGTNACC
3' CCANTGG
5' ---G   GTNACC--- 3'
3' ---CCANTG   G--- 5'
AspAI, Bse64I, BseT9I, BstPI, EcaI, Eci125I, EcoO65I, NspSAII
Bse118I [9] Bacillus sp. 118 5' RCCGGY
3' YGGCCR
5' ---R   CCGGY--- 3'
3' ---YGGCC   R--- 5'
Bse634I 1KNV Bacillus sp. 634 5' RCCGGY
3' YGGCCR
5' ---R   CCGGY--- 3'
3' ---YGGCC   R--- 5'
BseAI [10] Bacillus sphaericus 5' TCCGGA
3' AGGCCT
5' ---T   CCGGA--- 3'
3' ---AGGCC   T--- 5'
AccIII, Aor13HI, BlfI, BsiMI, Bsp13I, BspMII, CauB3I, Kpn2I
BseBI [11] Bacillus stearothermophilus 5' CCWGG
3' GGWCC
5' ---CC   WGG--- 3'
3' ---GGW   CC--- 5'
AjnI, AorI, Bse17I, Bst1I, BstOI, Bst2UI, EcoRII, MvaI, SleI, ZanI
BseCI [12] [13] [14] Bacillus sp. 5' ATCGAT
3' TAGCTA
5' ---AT   CGAT--- 3'
3' ---TAGC   TA--- 5'
AagI, BanIII, BavCI, Bsa29I, BspDI, Bsu15I, BsuTUI, ClaI, LcaI
BseDI Bacillus stearothermophilus RFL1434 5' CCNNGG
3' GGNNCC
5' ---C   CNNGG--- 3'
3' ---GGNNC   C--- 5'
Bse3DI Bacillus stearothermophilus 3D 5' GCAATG
3' CGTTAC
5' ---GCAATGNN   --- 3'
3' ---CGTTAC   NN--- 5'
BseGI Bacillus stearothermophilus Vs 34-031 5' GGATG
3' CCTAC
5' ---GGATGNN   --- 3'
3' ---CCTAC   NN--- 5'
BseJI Bacillus stearothermophilus Tsp5 5' GATN4ATC
3' CTAN4TAG
5' ---GATNN   NNATC--- 3'
3' ---CTANN   NNTAG--- 5'
BseKI Bacillus stearothermophilus Ra3-212 5' GCAGC
3' CGTCG
5' ---GCAGCN7N   NNNN--- 3'
3' ---CGTCGN7NNNNN   --- 5'
AlwXI, BbvI, BseXI, Bsp423I, Bst12I, Bst71I, BstV1I
BseLI Bacillus stearothermophilus LK3-551 5' CCN7GG
3' GGN7CC
5' ---CCNNNNN   NNGG--- 3'
3' ---GGNN   NNNNNCC--- 5'
BseMI Bacillus stearothermophilus Isl 15-111 5' GCAATG
3' CGTTAC
5' ---GCAATGNN   --- 3'
3' ---CGTTAC   NN--- 5'
BseMII [15] Bacillus stearothermophilus Isl 15-111 5' CTCAG
3' GAGTC
5' ---CTCAGN7NNN   --- 3'
3' ---GAGTCN7N   NN--- 5'
BseNI Bacillus sp. N 5' ACTGG
3' TGACC
5' ---ACTGGN   --- 3'
3' ---TGAC   CN--- 5'
BsePI Bacillus stearothermophilus P6 5' GCGCGC
3' CGCGCG
5' ---G   CGCGC--- 3'
3' ---CGCGC   G--- 5'
BseQI Bacillus sp. Q 5' GGCC
3' CCGG
5' ---GG   CC--- 3'
3' ---CC   GG--- 5'
BseRI [16] Bacillus sp. R 5' GAGGAG
3' CTCCTC
5' ---GAGGAGN7NNN   --- 3'
3' ---CTCCTCN7N   NN--- 5'
BseSI [17] Bacillus stearothermophilus Jo-553 5' GKGCMC
3' CMCGKG
5' ---GKGCM   C--- 3'
3' ---C   MCGKG--- 5'
BseT9I Bacillus sp. T9 5' GGTNACC
3' CCANTGG
5' ---G   GTNACC--- 3'
3' ---CCANTG   G--- 5'
AcrII, Bse64I, BstEII, BstT9I, EcaI, Eci125I, EcoO65I, NspSAII, PspEI
BseT10I Bacillus sp. T10 5' GGTNACC
3' CCANTGG
5' ---G   GTNACC--- 3'
3' ---CCANTG   G--- 5'
AcrII, AspAI, BstEII, BstT10I, BstPI, EcoO128I, EcoO65I, PspEI
BseXI Bacillus stearothermophilus Ra3-212 5' GCAGC
3' CGTCG
5' ---GCAGCN7N   NNNN--- 3'
3' ---CGTCGN7NNNNN   --- 5'
AlwXI, BbvI, BseKI, Bsp423I, Bst12I, Bst71I, BstV1I
BseX3I Bacillus stearothermophilus X3 5' CGGCCG
3' GCCGGC
5' ---C   GGCCG--- 3'
3' ---GCCGG   C--- 5'
AaaI, BstZI, EagI, EclXI, Eco52I, SenPT16I, XmaIII
BseYI Bacillus sp. 2521 5' CCCAGC
3' GGGTCG
5' ---C   CCAGC--- 3'
3' ---GGGTC   G--- 5'
BseZI Bacillus sp. Z 5' CTCTTC
3' GAGAAG
5' ---CTCTTCN   NNN--- 3'
3' ---GAGAAGNNNN   --- 5'
BsgI Bacillus sphaericus B922 5' GTGCAG
3' CACGTC
5' ---GTGCAGN12NNNN   --- 3'
3' ---CACGTCN12NN   NN--- 5'
BshI Bacillus sphaericus 5' GGCC
3' CCGG
5' ---GG   CC--- 3'
3' ---CC   GG--- 5'
Bsh45I Bacillus sphaericus 45 5' GWGCWC
3' CWCGWG
5' ---GWGCW   C--- 3'
3' ---C   WCGWG--- 5'
Alw21I, AspHI, Bbv12I, BsiHKAI,
HgiAI,  HpyF46II, MspV281I
Bsh1236I Bacillus sphaericus RFL1236 5' CGCG
3' GCGC
5' ---CG   CG--- 3'
3' ---GC   GC--- 5'
AccII, BceBI, BspFNI, Bsp50I, Csp68KVI, FauBII, MvnI, SelI
Bsh1285I Bacillus sphaericus RFL1285 5' CGRYCG
3' GCYRGC
5' ---CGRY   CG--- 3'
3' ---GC   YRGC--- 5'
Bsh1365I Bacillus sphaericus RFL1365 5' GATN4ATC
3' CTAN4TAG
5' ---GATNN   NNATC--- 3'
3' ---CTANN   NNTAG--- 5'
BshFI [18] Bacillus sphaericus 5' GGCC
3' CCGG
5' ---GG   CC--- 3'
3' ---CC   GG--- 5'
BshGI Bacillus sphaericus 5' CCWGG
3' GGWCC
5' ---CC   WGG--- 3'
3' ---GGW   CC--- 5'
AeuI, BseBI, Bse16I, Bst2I, BstNI, Bst38I, Bst100I, PspGI, SspAI
BshKI Bacillus sphaericus 5' GGNCC
3' CCNGG
5' ---G   GNCC--- 3'
3' ---CCNG   G--- 5'
AspS9I, Bac36I, BavAII, Bsp1894I, BspBII, CcuI, MaeK81II, Pde12I
BshNI Bacillus sphaericus TK-45 5' GGYRCC
3' CCRYGG
5' ---G   GYRCC--- 3'
3' ---CCRYG   G--- 5'
BanI, BbvBI, BspT107I, Eco64I, HgiCI, HgiHI, MspB4I, PfaAI
BshTI Bacillus sphaericus Jo22-024 5' ACCGGT
3' TGGCCA
5' ---A   CCGGT--- 3'
3' ---TGGCC   A--- 5'
AgeI, AsiAI,  AsiGI, CsiAI
CspAI, PinAI
BsiI [19] [20] Bacillus sphaericus 5' CACGAG
3' GTGCTC
5' ---C   ACGAG--- 3'
3' ---GTGCT   C--- 5'
BsiBI [21] Bacillus sp. 5' GATN4ATC
3' CTAN4TAG
5' ---GATNN   NNATC--- 3'
3' ---CTANN   NNTAG--- 5'
BsiCI Bacillus sp. 5' TTCGAA
3' AAGCTT
5' ---TT   CGAA--- 3'
3' ---AAGC   TT--- 5'
AcpI, AsuII, Bpu14I, BspT104I, Csp45I, FspII, NspV, PpaAI, SfuI
BsiEI [22] Bacillus sp. 5' CGRYCG
3' GCYRGC
5' ---CGRY   CG--- 3'
3' ---GC   YRGC--- 5'
BsiHKAI [23] Bacillus stearothermophilus 5' GWGCWC
3' CWCGWG
5' ---GWGCW   C--- 3'
3' ---C   WCGWG--- 5'
Alw21I, AspHI, Bbv12I, Bsh45I,
 Bsm6I, HgiAI, MspV281I
BsiHKCI Bacillus sp. HKC 5' CYCGRG
3' GRGCYC
5' ---C   YCGRG--- 3'
3' ---GRGCY   C--- 5'
AquI, BcoI, Bse15I, BspLU4I, Eco88I, Nli3877I, PlaAI, PunAI
BsiKI [24] Bacillus sp. 5' GGTNACC
3' CCANTGG
5' ---G   GTNACC--- 3'
3' ---CCANTG   G--- 5'
BsiLI Bacillus sp. 5' CCWGG
3' GGWCC
5' ---CC   WGG--- 3'
3' ---GGW   CC--- 5'
AglI, BseBI, Bse17I, Bst2I, BstNI, Bst38I, Bst100I, EcoRII, MvaI
BsiMI Bacillus sp. 5' TCCGGA
3' AGGCCT
5' ---T   CCGGA--- 3'
3' ---AGGCC   T--- 5'
AccIII, BbvAIII, BlfI, Bsp13I, BspMII, Bsu23I, Kpn2I, PinBII
BsiQI Bacillus sp. 5' TGATCA
3' ACTAGT
5' ---T   GATCA--- 3'
3' ---ACTAG   T--- 5'
AbaI, BclI, BspXII,
BstT7I, FbaI, Ksp22I,  ParI 
BsiSI [25] Bacillus sp. 5' CCGG
3' GGCC
5' ---C   CGG--- 3'
3' ---GGC   C--- 5'
BsiWI Bacillus sp. 5' CGTACG
3' GCATGC
5' ---C   GTACG--- 3'
3' ---GCATG   C--- 5'
BsiXI Bacillus sp. X 5' ATCGAT
3' TAGCTA
5' ---AT   CGAT--- 3'
3' ---TAGC   TA--- 5'
BscI, BsiXI, Bsp106I, BspDI, Bsu15I, BsuTUI, ClaI, Rme21I
BsiYI [26] Bacillus sp. 5' CCN7GG
3' GGN7CC
5' ---CCNNNNN   NNGG--- 3'
3' ---GGNN   NNNNNCC--- 5'
BsiZI Bacillus sp. 5' GGNCC
3' CCNGG
5' ---G   GNCC--- 3'
3' ---CCNG   G--- 5'
AspS9I, Bac36I, BavAII, BshKI, BspBII, CcuI, MaeK81II, Pde12I
BslI Bacillus sp. 5' CCN7GG
3' GGN7CC
5' ---CCNNNNN   NNGG--- 3'
3' ---GGNN   NNNNNCC--- 5'
BslFI Bacillus stearothermophilus FI 5' GGGAC
3' CCCTG
5' ---GGGACN8NN   NNNN--- 3'
3' ---CCCTGN8NNNNNN   --- 5'
BsmI Bacillus stearothermophilus NUB 5' GAATGC
3' CTTACG
5' ---GAATGCN   --- 3'
3' ---CTTAC   GN--- 5'
Asp26HI, Asp36HI, Asp40HI, BmaHI, BscCI, Mva1269I, PctI
BsmAI Bacillus stearothermophilus A664 5' GTCTC
3' CAGAG
5' ---GTCTCN   NNNN--- 3'
3' ---CAGAGNNNNN   --- 5'
Alw26I,  BcoDI, BscQII, BsoMAI,
BstMAI
BsmBI Bacillus stearothermophilus B61 5' CGTCTC
3' GCAGAG
5' ---CGTCTCN   NNNN--- 3'
3' ---GCAGAGNNNNN   --- 5'
BsmFI Bacillus stearothermophilus F 5' GGGAC
3' CCCTG
5' ---GGGACN8NN   NNNN--- 3'
3' ---CCCTGN8NNNNNN   --- 5'
BsmSI Bacillus stearothermophilus CP114 5' CCWWGG
3' GGWWCC
5' ---C   CWWGG--- 3'
3' ---GGWWC   C--- 5'
Bso31I Bacillus stearothermophilus E31 5' GGTCTC
3' CCAGAG
5' ---GGTCTCN   NNNN--- 3'
3' ---CCAGAGNNNNN   --- 5'
BsoBI 1DC1 Bacillus stearothermophilus JN2091 5' CYCGRG
3' GRGCYC
5' ---C   YCGRG--- 3'
3' ---GRGCY   C--- 5'
AquI, BcoI, BsiHKCI, BspLU4I, Eco88I, Nli3877I, PlaAI, PunAI
BsoCI Bacillus stearothermophilus AU891 5' GDGCHC
3' CHCGDG
5' ---GDGCH   C--- 3'
3' ---C   HCGDG--- 5'
AocII, BmyI, Bsp1286I, BspLS2I, MhlI, NspII, SduI
BsoFI Bacillus stearothermophilus F66 5' GCNGC
3' CGNCG
5' ---GC   NGC--- 3'
3' ---CGN   CG--- 5'
BsoMAI Bacillus stearothermophilus MA 5' GTCTC
3' CAGAG
5' ---GTCTCN   NNNN--- 3'
3' ---CAGAGNNNNN   --- 5'
Alw26I,  BcoDI, BscQII, BsmAI,
BstMAI

Notes

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  14. Rina M, Dialektakis D, Clark D, Pagomenou M, Bouriotis V (April 1992). "Isolation and identification of restriction endonuclease BseCI". Nucleic Acids Res. 20 (7): 1807. doi:10.1093/nar/20.7.1807. PMC   312279 . PMID   1579477.
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  19. Degtyarev SK, Rechkunova NI, Kolyhalov AA, Dedkov VS, Zhilkin PA (October 1990). "II-Q restriction endonucleases--new class of type II enzymes". Nucleic Acids Res. 18 (19): 5807–10. doi:10.1093/nar/18.19.5807. PMC   332318 . PMID   2216771.
  20. Degtiarev SKh; Kolykhalov AA; Rechkunova NI; Dedkov VS; Zhilkin PA (January–February 1990). "BsiI--a new unusual restriction endonuclease". Mol Biol (Mosk) (in Russian). 24 (1): 244–7. PMID   2348824.
  21. Mok YK, Clark DR, Kam KM, Shaw PC (November 1990). "Restriction endonuclease from thermophilic bacterial species. II. Isolation and characterization of BsiBI". Nucleic Acids Res. 18 (22): 6740. doi:10.1093/nar/18.22.6740. PMC   332681 . PMID   2251161.
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  25. Rina M, Bouriotis V (March 1990). "Isolation and identification of restriction endonuclease BsiSI". Nucleic Acids Res. 18 (6): 1654. doi:10.1093/nar/18.6.1654. PMC   330560 . PMID   1691485.
  26. Mok YK, Clark DR, Kam KM, Shaw PC (May 1991). "BsiY I, a novel thermophilic restriction endonuclease that recognizes 5' CCNNNNNNNGG 3' and the discovery of a wrongly sequenced site in pACYC177". Nucleic Acids Res. 19 (9): 2321–3. doi:10.1093/nar/19.9.2321. PMC   329437 . PMID   2041772.

Related Research Articles

A restriction enzyme, restriction endonuclease, REase, ENase orrestrictase is an enzyme that cleaves DNA into fragments at or near specific recognition sites within molecules known as restriction sites. Restriction enzymes are one class of the broader endonuclease group of enzymes. Restriction enzymes are commonly classified into five types, which differ in their structure and whether they cut their DNA substrate at their recognition site, or if the recognition and cleavage sites are separate from one another. To cut DNA, all restriction enzymes make two incisions, once through each sugar-phosphate backbone of the DNA double helix.

<i>Nla</i>III Enzyme

NlaIII is a type II restriction enzyme isolated from Neisseria lactamica. As part of the restriction modification system, NlaIII is able to prevent foreign DNA from integrating into the host genome by cutting double stranded DNA into fragments at specific sequences. This results in further degradation of the fragmented foreign DNA and prevents it from infecting the host genome.

Site-specific DNA-methyltransferase (cytosine-N4-specific) is an enzyme with systematic name S-adenosyl-L-methionine:DNA-cytosine N4-methyltransferase. This enzyme catalyses the following chemical reaction:

<span class="mw-page-title-main">Homing endonuclease</span>

The homing endonucleases are a collection of endonucleases encoded either as freestanding genes within introns, as fusions with host proteins, or as self-splicing inteins. They catalyze the hydrolysis of genomic DNA within the cells that synthesize them, but do so at very few, or even singular, locations. Repair of the hydrolyzed DNA by the host cell frequently results in the gene encoding the homing endonuclease having been copied into the cleavage site, hence the term 'homing' to describe the movement of these genes. Homing endonucleases can thereby transmit their genes horizontally within a host population, increasing their allele frequency at greater than Mendelian rates.

PstI is a type II restriction endonuclease isolated from the Gram negative species, Providencia stuartii.

In molecular biology, the BsuBI/PstI restriction endonuclease family is a family of type II restriction endonucleases. It includes BsuBI and PstI. The enzymes of the BsuBI restriction/modification (R/M) system recognise the target sequence 5'CTGCAG and are functionally identical with those of the PstI R/M system.