List of restriction enzyme cutting sites: Bsp–Bss

Last updated January 20, 2023

Legend of nucleobases
Code	Nucleotide represented
A	Adenine (A)
C	Cytosine (C)
G	Guanine (G)
T	Thymine (T)
N	A, C, G or T
M	A or C
R	A or G
W	A or T
Y	C or T
S	C or G
K	G or T
H	A, C or T
B	C, G or T
V	A, C or G
D	A, G or T

This article contains a list of the most studied restriction enzymes whose names start with Bsp to Bss inclusive. It contains approximately 180 enzymes.

Enzyme: Accepted name of the molecule, according to the internationally adopted nomenclature ^[1]^[2], and bibliographical references. (Further reading: see the section "Nomenclature" in the article "Restriction enzyme".)
PDB code: Code used to identify the structure of a protein in the PDB database of protein structures. The 3D atomic structure of a protein provides highly valuable information to understand the intimate details of its mechanism of action^[3]^[4].
Source: Organism that naturally produces the enzyme.
Recognition sequence : Sequence of DNA recognized by the enzyme and to which it specifically binds.
Cut: Cutting site and DNA products of the cut. The recognition sequence and the cutting site usually match, but sometimes the cutting site can be dozens of nucleotides away from the recognition site^[5]^[6].
Isoschizomers and neoschizomers: An isoschizomer is an enzyme that recognizes the same sequence as another. A neoschizomer is a special type of isoschizomer that recognizes the same sequence as another, but cuts in a different manner. A maximum number of 8-10 most common isoschizomers are indicated for every enzyme but there may be many more. Neoschizomers are shown in bold and green color font (e.g.: BamHI). When "None on date" is indicated, that means that there were no registered isoschizomers in the databases on that date with a clearly defined cutting site. Isoschizomers indicated in white font and grey background correspond to enzymes not listed in the current lists:

as in this not listed enzyme:

EcoR70I

Whole list navigation

Restriction enzymes

Bsp

Enzyme PDB code Source Recognition sequence Cut Isoschizomers

Bsp6I Bacillus sp. RFL6 5' GCNGC
3' CGNCG 5' ---GC NGC--- 3'
3' ---CGN CG--- 5'

Bsp13I Bacillus sp. 13 5' TCCGGA
3' AGGCCT 5' ---T CCGGA--- 3'
3' ---AGGCC T--- 5' AccIII, BbvAIII, BlfI, BsiMI, BspMII, Bsu23I, Kpn2I, PinBII

Bsp19I Bacillus sp. 19 5' CCATGG
3' GGTACC 5' ---C CATGG--- 3'
3' ---GGTAC C--- 5'

Bsp24I Bacillus sp. 24 5' GACN₆TGG
3' CTGN₆ACC 5' ---GACN₆TGGN₆NNNNNN --- 3'
3' ---CTGN₆ACCN₆N NNNNN--- 5'

Bsp50I Bacillus sp. RFL50 5' CGCG
3' GCGC 5' ---CG CG--- 3'
3' ---GC GC--- 5' BceBI, Bsh1236I, BstFNI, BstUI, Bsu1532I, FalII, FnuDII, MvnI, ThaI

Bsp63I Bacillus sphaericus 63 5' CTGCAG
3' GACGTC 5' ---CTGCA G--- 3'
3' ---G ACGTC--- 5' AjoI, Asp713I, BloHII, MhaAI, Pfl21I, PstI, SalPI, SflI, Sst12I, YenI

Bsp67I Bacillus sphaericus 67 5' GATC
3' CTAG 5' --- GATC--- 3'
3' ---CTAG --- 5' AspMDI, Bsp143I, BspJI, BtkII, DpnII, LlaAI, NlaII, SsiBI

Bsp68I Bacillus sp. RFL68 5' TCGCGA
3' AGCGCT 5' ---TCG CGA--- 3'
3' ---AGC GCT--- 5' NruI

Bsp98I Bacillus sp. RFL98 5' GGATCC
3' CCTAGG 5' ---G GATCC--- 3'
3' ---CCTAG G--- 5' AccEBI, AliI, BamHI, BnaI, BstI, CelI, OkrAI, NspSAIV, Pfl8I, SurI

Bsp105I Bacillus sphaericus 105 5' GATC
3' CTAG 5' --- GATC--- 3'
3' ---CTAG --- 5' Bfi57I, Bsp143I, BspJI, BtkII, DpnII, LlaAI, NlaII, SsiBI

Bsp106I Bacillus sphaericus 106 5' ATCGAT
3' TAGCTA 5' ---AT CGAT--- 3'
3' ---TAGC TA--- 5' BbvAII, Bci29I, BciBI, BcmI, BspOVII, BstNZ169I, Rme21I

Bsp119I Bacillus sp. RFL119 5' TTCGAA
3' AAGCTT 5' ---TT CGAA--- 3'
3' ---AAGC TT--- 5' AcpI, Bim19I, Bpu14I, BspT104I, Csp45I, FspII, NspV, SspRFI

Bsp120I Bacillus sp. RFL120 5' GGGCCC
3' CCCGGG 5' ---G GGCCC--- 3'
3' ---CCCGG G--- 5' ApaI, PpeI, PspOMI

Bsp123I Bacillus sp. 123 5' CGCG
3' GCGC 5' ---CG CG--- 3'
3' ---GC GC--- 5' BceBI, Bsh1236I, BstFNI, BstUI, BtkI, Csp68KVI, FnuDII, MvnI, ThaI

Bsp143I Bacillus sp. RFL143 5' GATC
3' CTAG 5' --- GATC--- 3'
3' ---CTAG --- 5' Bce243I, Bsp105I, BspFI, BstKTI, CpfI, HacI, NdeII, Sth368I, Sau3AI

Bsp143II

Bacillus sp. RFL143

5' RGCGCY
3' YCGCGR

5' ---RGCGC Y--- 3'
3' ---Y CGCGR--- 5'

AccB2I,	BfoI,	Bme142I, BstH2I,
HaeII, LpnI

Bsp211I Bacillus sphaericus 211 5' GGCC
3' CCGG 5' ---GG CC--- 3'
3' ---CC GG--- 5'

Bsp423I Bacillus sp. 423 5' GCAGC
3' CGTCG 5' ---GCAGCN₇N NNNN--- 3'
3' ---CGTCGN₇NNNNN --- 5' AlwXI, BbvI, BseKI, BseXI, Bst12I, Bst71I, BstV1I

Bsp519I Bacillus sp. 519 5' GRGCYC
3' CYCGRG 5' ---GRGCY C--- 3'
3' ---C YCGRG--- 5'

Bsp1286I Bacillus sphaericus 5' GDGCHC
3' CHCGDG 5' ---GDGCH C--- 3'
3' ---C HCGDG--- 5' AocII, BmyI, BsoCI, Bsp1286I, BspLS2I, NspII, SduI

Bsp1407I

Bacillus stearothermophilus RFL1407

5' TGTACA
3' ACATGT

5' ---T GTACA--- 3'
3' ---ACATG T--- 5'

AauI,	BsmRI,	BsrGI, BstAUI,
Ssp4800I, SspBI

Bsp1720I Bacillus sp. 1720 5' GCTNAGC
3' CGANTCG 5' ---GC TNAGC--- 3'
3' ---CGANT CG--- 5'

Bsp1894I Bacillus sphaericus 1894 5' GGNCC
3' CCNGG 5' ---G GNCC--- 3'
3' ---CCNG G--- 5' AspS9I, Bac36I, BavBII, BshKI, BspBII, CcuI, MaeK81II, Pde12I

Bsp2095I Bacillus sphaericus 2095 5' GATC
3' CTAG 5' --- GATC--- 3'
3' ---CTAG --- 5' Bfi57I, Bsp143I, BspKT6I, BtkII, DpnII, LlaAI, NlaII, SsiBI

Bsp4009I Bacillus sp. 4009 5' GGATCC
3' CCTAGG 5' ---G GATCC--- 3'
3' ---CCTAG G--- 5' AccEBI, AliI, ApaCI, BamHI, BstI, CelI, OkrAI, Pfl8I, RspLKII, SurI

BspAI Bacillus sphaericus JL4B 5' GATC
3' CTAG 5' --- GATC--- 3'
3' ---CTAG --- 5' Bfi57I, Bsp2095I, BspKT6I, BtkII, DpnII, LlaAI, NlaII, SsiBI

BspA2I Bacillus sp. A2 5' CCTAGG
3' GGATCC 5' ---C CTAGG--- 3'
3' ---GGATC C--- 5' AspA2I, AvrII, AvrBII, BlnI, XmaJI

Bsp153AI Bacillus sp. 153A 5' CAGCTG
3' GTCGAC 5' ---CAG CTG--- 3'
3' ---GTC GAC--- 5'

BspAAI Bacillus sp. AA 5' CTCGAG
3' GAGCTC 5' ---C TCGAG--- 3'
3' ---GAGCT C--- 5' AbrI, BluI, BstVI, PanI, SauLPII, SciI, SlaI, StrI, TliI

BspAAII Bacillus sp. AA 5' TCTAGA
3' AGATCT 5' ---T CTAGA--- 3'
3' ---AGATC T--- 5'

BspAAIII Bacillus sp. AA 5' GGATCC
3' CCTAGG 5' ---G GATCC--- 3'
3' ---CCTAG G--- 5' AccEBI, AliI, ApaCI, AsiI, BamHI, Bce751I, Pfl8I, RspLKII, SolI

BspANI Bacillus sp. AN 5' GGCC
3' CCGG 5' ---GG CC--- 3'
3' ---CC GG--- 5'

BspBI Bacillus sphaericus JL14 5' CTGCAG
3' GACGTC 5' ---CTGCA G--- 3'
3' ---G ACGTC--- 5' AjoI, ApiI, Asp713I, BloHII, CfrA4I, Pfl21I, PstI, SalPI, Sst12I, YenI

BspBII Bacillus sphaericus JL14 5' GGNCC
3' CCNGG 5' ---G GNCC--- 3'
3' ---CCNG G--- 5' AsuI, Bac36I, BavBII, BshKI, BspF4I, CcuI, MaeK81II, PspPI

BspBRI Bacillus sp. 5' GGCC
3' CCGG 5' ---GG CC--- 3'
3' ---CC GG--- 5'

BspBS31I Bacillus sp. BS31 5' GAAGAC
3' CTTCTG 5' ---GAAGACNN NNNN--- 3'
3' ---CTTCTGNNNNNN --- 5'

BspCI Bacillus sp. C1 5' CGATCG
3' GCTAGC 5' ---CGAT CG--- 3'
3' ---GC TAGC--- 5' Afa16RI, Afa22MI, EagBI, ErhB9I, NblI, Psu161I, RshI, XorII

BspCNI Bacillus sp. 1310 5' CTCAG
3' GAGTC 5' ---CTCAGN₆NNN --- 3'
3' ---GAGTCN₆N NN--- 5'

BspDI Bacillus sp. 5' ATCGAT
3' TAGCTA 5' ---AT CGAT--- 3'
3' ---TAGC TA--- 5' AagI, BanIII, BavCI, Bsa29I, BseCI, Bsu15I, BsuTUI, ClaI, ZhoI

BspD6I ^[7]^[8] 2P14 Bacillus sp. D6 5' GACTC
3' CTGAG 5' ---GACTCNNNN NN--- 3'
3' ---CTGAGNNNNNN --- 5'

BspEI Bacillus sp. 5' TCCGGA
3' AGGCCT 5' ---T CCGGA--- 3'
3' ---AGGCC T--- 5' AccIII, BlfI, BsiMI, Bsp13I, BspMII, CauB3I, MroI, PinBII

BspFI Bacillus sp. 5' GATC
3' CTAG 5' --- GATC--- 3'
3' ---CTAG --- 5' Bfi57I, Bsp2095I, BspKT6I, BtkII, DpnII, LlaAI, NlaII, SsiBI

BspF4I Bacillus sp. F4 5' GGNCC
3' CCNGG 5' ---G GNCC--- 3'
3' ---CCNG G--- 5' AsuI, Bac36I, BavBII, BsiZI, BspBII, CcuI, MaeK81II, PspPI

BspHI Bacillus sp. H3 5' TCATGA
3' AGTACT 5' ---T CATGA--- 3'
3' ---AGTAC T--- 5'

BspIS4I Bacillus sp. IS4 5' GAAGAC
3' CTTCTG 5' ---GAAGACNN NNNN--- 3'
3' ---CTTCTGNNNNNN --- 5'

BspJI Bacillus sp. 5' GATC
3' CTAG 5' --- GATC--- 3'
3' ---CTAG --- 5' BfuCI, Bsp2095I, BspKT6I, BtkII, DpnII, LlaAI, NmeCI, SsiAI

BspJII Bacillus sp. 5' ATCGAT
3' TAGCTA 5' ---AT CGAT--- 3'
3' ---TAGC TA--- 5' Bsa29I, BseCI, Bsp106I, BspDI, BspOVII, BspXI, BspZEI

BspKI Bacillus sp. K 5' GGCC
3' CCGG 5' ---GG CC--- 3'
3' ---CC GG--- 5'

BspKT5I Bacillus sp. 5' CTGAAG
3' GACTTC 5' ---CTGAAGN₁₂NNNN --- 3'
3' ---GACTTCN₁₂NN NN--- 5'

BspKT6I Bacillus sp. KT6 5' GATC
3' CTAG 5' ---GAT C--- 3'
3' ---C TAG--- 5' BfuCI, Bsp2095I, Bst19II, BstKTI, DpnII, LlaAI, NmeCI, SsiAI

BspKT8I Bacillus sp. KT8 5' AAGCTT
3' TTCGAA 5' ---A AGCTT--- 3'
3' ---TTCGA A--- 5'

BspLI

Bacillus sp. RJ3-212

5' GGNNCC
3' CCNNGG

5' ---GGN NCC--- 3'
3' ---CCN NGG--- 5'

AspNI, BscBI,	BmiI,	NlaIV,
PspN4I

BspLAI Bacillus sp. LA 5' GCGC
3' CGCG 5' ---GCG C--- 3'
3' ---C GCG--- 5' AspLEI, BstHHI, CfoI, FnuDIII, HhaI, Hin6I, HinP1I, HsoI, HspAI, SciNI

BspLAII Bacillus sp. LA 5' TTCGAA
3' AAGCTT 5' ---TT CGAA--- 3'
3' ---AAGC TT--- 5'

BspLAIII Bacillus sp. LA 5' AAGCTT
3' TTCGAA 5' ---A AGCTT--- 3'
3' ---TTCGA A--- 5'

BspLS2I Bacillus sp. LS2 5' GDGCHC
3' CHCGDG 5' ---GDGCH C--- 3'
3' ---C HCGDG--- 5' AocII, BmyI, BsoCI, BspLS2I, MhlI, NspII, SduI

BspLU4I Bacillus sp. LU4 5' CYCGRG
3' GRGCYC 5' ---C YCGRG--- 3'
3' ---GRGCY C--- 5' Ama87I, AvaI, Bse15I, BsoBI, BstSI, NspIII, NspSAI, OfoI

BspLU11I Bacillus sp. LU11 5' ACATGT
3' TGTACA 5' ---A CATGT--- 3'
3' ---TGTAC A--- 5'

BspLU11III Bacillus sp. LU11 5' GGGAC
3' CCCTG 5' ---GGGACN₈NN NNNN--- 3'
3' ---CCCTGN₈NNNNNN --- 5'

BspMI Bacillus sp. M 5' ACCTGC
3' TGGACG 5' ---ACCTGCNNNN NNNN--- 3'
3' ---TGGACGNNNNNNNN --- 5' Acc36I, BfuAI, BveI

BspMII Bacillus sp. M 5' TCCGGA
3' AGGCCT 5' ---T CCGGA--- 3'
3' ---AGGCC T--- 5' AccIII, BbvAIII, BlfI, BsiMI, BspEI, Bsu23I, CauB3I, MroI

BspM39I Bacillus sp. M39 5' CAGCTG
3' GTCGAC 5' ---CAG CTG--- 3'
3' ---GTC GAC--- 5'

BspM90I Bacillus sp. M90 5' GTATAC
3' CATATG 5' ---GTA TAC--- 3'
3' ---CAT ATG--- 5'

BspMAI Bacillus stearothermophilus MA 5' CTGCAG
3' GACGTC 5' ---CTGCA G--- 3'
3' ---G ACGTC--- 5' AjoI, Asp713I, BloHII, PaePI, Psp23I, PstI, SalPI, Sst12I, YenI

BspMKI Bacillus sp. MKI 5' GTCGAC
3' CAGCTG 5' ---G TCGAC--- 3'
3' ---CAGCT G--- 5'

BspNI Bacillus sp. N 5' CCWGG
3' GGWCC 5' ---CC WGG--- 3'
3' ---GGW CC--- 5' AglI, BseBI, Bse17I, Bst2I, CbrI, CthII, EcoRII, MvaI, SspAI, TaqXI

BspO4I Bacillus sp. O-4 5' CAGCTG
3' GTCGAC 5' ---CAG CTG--- 3'
3' ---GTC GAC--- 5'

BspOVI

Bacillus sp. OV

5' GACN₅GTC
3' CTGN₅CAG

5' ---GACNNN NNGTC--- 3'
3' ---CTGNN NNNCAG--- 5'

AhdI, AspEI,	BmeRI,	DriI,
Eam1105I, EclHKI, NruGI

BspOVII Bacillus sp. OV 5' ATCGAT
3' TAGCTA 5' ---AT CGAT--- 3'
3' ---TAGC TA--- 5' BanIII, BavCI, BciBI, BcmI, Bli86I, BliRI, BspJII, BstNZ169I, SpmI

BspPI

Bacillus sp. d 1-34

5' GGATC
3' CCTAG

5' ---GGATCNNNN N--- 3'
3' ---CCTAGNNNNN --- 5'

AclWI, AlwI, BinI,

BsrWI,

BstH9I,

Bst31TI, Bth617I,

EacI

BspRI Bacillus sphaericus R 5' GGCC
3' CCGG 5' ---GG CC--- 3'
3' ---CC GG--- 5'

BspR7I Bacillus sp. R7 5' CCTNAGG
3' GGANTCC 5' ---CC TNAGG--- 3'
3' ---GGANT CC--- 5' AocI, AxyI, Bse21I, Bsu36I, Eco81I, MstII, SauI, SshAI

BspST5I Bacillus sp. ST5 5' GCATC
3' CGTAG 5' ---GCATCN₄N NNNN--- 3'
3' ---CGTAGN₄NNNNN --- 5'

BspTI Bacillus sp. RFL1265 5' CTTAAG
3' GAATTC 5' ---C TTAAG--- 3'
3' ---GAATT C--- 5' AflII

BspT104I Bacillus sp. T104 5' TTCGAA
3' AAGCTT 5' ---TT CGAA--- 3'
3' ---AAGC TT--- 5' AcpI, Bim19I, Bpu14I, BstBI, Csp45I, LspI, NspV, SspRFI

BspT107I Bacillus sp. T107 5' GGYRCC
3' CCRYGG 5' ---G GYRCC--- 3'
3' ---CCRYG G--- 5' AccB1I, BanI, BbvBI, Eco64I, HgiCI, HgiHI, MspB4I, PfaAI

BspTNI Bacillus sp. TN 5' GGTCTC
3' CCAGAG 5' ---GGTCTCN NNNN--- 3'
3' ---CCAGAGNNNNN --- 5'

BspTS514I Bacillus sp. TS514 5' GAAGAC
3' CTTCTG 5' ---GAAGACNN NNNN--- 3'
3' ---CTTCTGNNNNNN --- 5'

BspWI Bacillus sp. 3456 5' GCN₇GC
3' CGN₇CG 5' ---GCNNNNN NNGC--- 3'
3' ---CGNN NNNNNCG--- 5'

BspXI Bacillus sphaericus X 5' ATCGAT
3' TAGCTA 5' ---AT CGAT--- 3'
3' ---TAGC TA--- 5' BliAI, BliRI, BscI, Bst28I, Bsu15I, LcaI, PgaI, Ssp27144I, ZhoI

BspXII

Bacillus sphaericus X

5' TGATCA
3' ACTAGT

5' ---T GATCA--- 3'
3' ---ACTAG T--- 5'

AbaI, BclI, BsiQI,
BstT7I, FbaI, Ksp22I,	ParI

BspZEI Bacillus sp. ZE 5' ATCGAT
3' TAGCTA 5' ---AT CGAT--- 3'
3' ---TAGC TA--- 5' Bsa29I, BseCI, BspDI, BspXI, Bst28I, BstNZ169I, BsuTUI, LplI

Bsr - Bss

Enzyme PDB code Source Recognition sequence Cut Isoschizomers

BsrI Bacillus stearothermophilus 5' ACTGG
3' TGACC 5' ---ACTGGN --- 3'
3' ---TGAC CN--- 5'

BsrAI Bacillus stearothermophilus CPW7 5' GGWCC
3' CCWGG 5' ---G GWCC--- 3'
3' ---CCWG G--- 5' BamNxI, BcuAI, Csp68KI, Eco47I, FssI, HgiCII, HgiJI, SinI

BsrBI Bacillus stearothermophilus CPW193 5' CCGCTC
3' GGCGAG 5' ---CCG CTC--- 3'
3' ---GGC GAG--- 5' AccBSI, BstD102I, Bst31NI, MbiI

BsrBRI Bacillus stearothermophilus BR 5' GATN₄ATC
3' CTAN₄TAG 5' ---GATNN NNATC--- 3'
3' ---CTANN NNTAG--- 5'

BsrDI Bacillus stearothermophilus D70 5' GCAATG
3' CGTTAC 5' ---GCAATGNN --- 3'
3' ---CGTTAC NN--- 5'

BsrFI Bacillus stearothermophilus CPW16 5' RCCGGY
3' YGGCCR 5' ---R CCGGY--- 3'
3' ---YGGCC R--- 5'

BsrGI

Bacillus stearothermophilus GR75

5' TGTACA
3' ACATGT

5' ---T GTACA--- 3'
3' ---ACATG T--- 5'

AauI,	BsmRI,	Bsp1407I, BstAUI,
Ssp4800I, SspBI

BsrSI Bacillus stearothermophilus CPW19 5' ACTGG
3' TGACC 5' ---ACTGGN --- 3'
3' ---TGAC CN--- 5'

BssAI Bacillus sp. 5' RCCGGY
3' YGGCCR 5' ---R CCGGY--- 3'
3' ---YGGCC R--- 5'

BssECI Bacillus stearothermophilus EC 5' CCNNGG
3' GGNNCC 5' ---C CNNGG--- 3'
3' ---GGNNC C--- 5'

BssHI Bacillus stearothermophilus H3 5' CTCGAG
3' GAGCTC 5' ---C TCGAG--- 3'
3' ---GAGCT C--- 5' AbrI, BluI, BstVI, PanI, SauLPII, SciI, SlaI, StrI, TliI

BssHII Bacillus stearothermophilus H3 5' GCGCGC
3' CGCGCG 5' ---G CGCGC--- 3'
3' ---CGCGC G--- 5'

BssIMI Bacillus stearothermophilus IM 5' GGGTC
3' CCCAG 5' ---GG GTC--- 3'
3' ---CCCAG --- 5'

BssKI Bacillus stearothermophilus TBI 5' CCNGG
3' GGNCC 5' --- CCNGG--- 3'
3' ---GGNCC --- 5'

BssNAI Bacillus stearothermophilus NA 5' GTATAC
3' CATATG 5' ---GTA TAC--- 3'
3' ---CAT ATG--- 5'

BssNI Bacillus sphaericus KTN 5' GRCGYC
3' CYGCRG 5' ---GR CGYC--- 3'
3' ---CYGC RG--- 5' AcyI, AhaII, AosII, AstWI, AsuIII, BsaHI, HgiI, Hsp92I, Msp17I

BssSI Bacillus stearothermophilus S719 5' CACGAG
3' GTGCTC 5' ---C ACGAG--- 3'
3' ---GTGCT C--- 5'

BssT1I Bacillus stearothermophilus T1 5' CCWWGG
3' GGWWCC 5' ---C CWWGG--- 3'
3' ---GGWWC C--- 5'

Notes

↑ Smith HO, Nathans D (December 1973). "Letter: A suggested nomenclature for bacterial host modification and restriction systems and their enzymes". J. Mol. Biol. 81 (3): 419–23. doi:10.1016/0022-2836(73)90152-6. PMID 4588280.
↑ Roberts RJ, Belfort M, Bestor T, Bhagwat AS, Bickle TA, Bitinaite J, Blumenthal RM, Degtyarev SK, Dryden DT, Dybvig K, Firman K, Gromova ES, Gumport RI, Halford SE, Hattman S, Heitman J, Hornby DP, Janulaitis A, Jeltsch A, Josephsen J, Kiss A, Klaenhammer TR, Kobayashi I, Kong H, Krüger DH, Lacks S, Marinus MG, Miyahara M, Morgan RD, Murray NE, Nagaraja V, Piekarowicz A, Pingoud A, Raleigh E, Rao DN, Reich N, Repin VE, Selker EU, Shaw PC, Stein DC, Stoddard BL, Szybalski W, Trautner TA, Van Etten JL, Vitor JM, Wilson GG, Xu SY (April 2003). "A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes". Nucleic Acids Res. 31 (7): 1805–12. doi:10.1093/nar/gkg274. PMC 152790 . PMID 12654995.
↑ Jeremy MB, John LT, Lubert S (2002). "3. Protein Structure and Function". Biochemistry. San Francisco: W. H. Freeman. ISBN 0-7167-4684-0.
↑ Anfinsen C.B. (1973). "Principles that Govern the Folding of Protein Chains". Science. 181 (4096): 223–30. doi:10.1126/science.181.4096.223. PMID 4124164.
↑ Kessler C, Manta V (August 1990). "Specificity of restriction endonucleases and DNA modification methyltransferases a review (Edition 3)". Gene. 92 (1–2): 1–248. doi:10.1016/0378-1119(90)90486-B. PMID 2172084.
↑ Pingoud A, Jeltsch A (September 2001). "Structure and function of type II restriction endonucleases". Nucleic Acids Res. 29 (18): 3705–27. doi:10.1093/nar/29.18.3705. PMC 55916 . PMID 11557805.
↑ Kachalova GS, Rogulin EA, Yunusova AK, Artyukh RI, Perevyazova TA, Matvienko NI, Zheleznaya LA, Bartunik HD (December 2008). "Structural analysis of the heterodimeric type IIS restriction endonuclease R.BspD6I acting as a complex between a monomeric site-specific nickase and a catalytic subunit". J Mol Biol. 384 (2): 489–502. doi:10.1016/j.jmb.2008.09.033. PMID 18835275.
↑ Kachalova GS, Rogulin EA, Yunusova AK, Artyukh RI, Perevyazova TA, Matvienko NI, Zheleznaya LA, Bartunik HD (September 2007). "Crystallization and preliminary X-ray diffraction analysis of the small subunit of the heterodimeric restriction endonuclease R.BspD6I". Acta Crystallogr F. 63 (Pt 9): 795–7. doi:10.1107/S174430910704016X. PMC 2376331 . PMID 17768358.

v t e Restriction modification system: restriction enzyme
Basic Concept	Isoschizomer Neoschizomer Isocaudomer
Recognition sequence 4bp	TaqI Sau3A AluI* HaeIII* DpnII NlaIII HpaII
Recognition sequence 5bp	EcoRII FokI
Recognition sequence 6bp	AaaI BglII PovII* EcoRV* EcoRI BamHI HindIII XbaI XhoI SacI PstI NdeI
Recognition sequence 8bp	NotI
Lists	A Ba–Bc Bd–Bp Bsa–Bso Bsp–Bss Bst–Bv C-D E-F G-K L-N O-R S T-Z
* means cleavage produces blunt ends

List of restriction enzyme cutting sites: Bsp–Bss

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Restriction enzymes

Bsp

Bsr - Bss

Notes

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