Lucilia coeruleiviridis

Last updated

Lucilia coeruleiviridis
Lucilia coeruleiviridis.jpg
Scientific classification Red Pencil Icon.png
Kingdom: Animalia
Phylum: Arthropoda
Class: Insecta
Order: Diptera
Family: Calliphoridae
Genus: Lucilia
Species:
L. coeruleiviridis
Binomial name
Lucilia coeruleiviridis
Macquart, 1855
Synonyms
  • Phaenicia caeruleiviridis Macquart, 1855
  • Lucilia australisTownsend, 1908
  • Lucilia oculataTownsend, 1908

Lucilia coeruleiviridis, formerly Phaenecia coeruleiviridis, is commonly known as a green bottle fly, because of its metallic blue-green thorax and abdomen. L. coeruleiviridis was first discovered by French entomologist Pierre-Justin-Marie Macquart in 1855. It belongs to the family Calliphoridae and is one of many forensically important Diptera, as it is often found on decaying substances. L. coeruleiviridis is one of the most ubiquitous blow fly species in the southeastern United States, particularly in the spring and fall months. [1]

Contents

Taxonomy

The name L. caeruleiviridis may be a contraction of the Latin words "caerulei" and "viridis". Respectively, these words mean cerulean blue, and greenish and refer to the color of the fly's body.

Description

Adult appearance

Like all green bottle flies in its family, the Lucilia coeruleiviridis adult is a metallic blue-green bodied fly. The facial region is white with large red compound eyes. There are also bristles present as well as plumose aristae. The thorax also contains bristles, all of which are evenly paired. Just behind the head, the anterior spiracle is black in color, as is the thoracic posterior spiracle. The meron, just below the wing, is bristled. The venation of the wings is “incomplete” in that it does not reach the wing edge. The basicosta of the wing, or the “shoulder” area, is yellow in coloration, and the calypters—the scale-like structures just below the wing base—are white and of unequal size. The legs of the adult are usually brown to black in color. Like most flies, it also has tarsal pulvilli, soft pads at the end of each foot used to “stick” to surfaces; in this species they are slightly yellow in color. As with all insects, coloration is very important in identification of a species, as is the presence of bristles. Sometimes, the presence of a pair of bristles on the thoracic plate is the only reliable way to distinguish one species from another. [2]

Larval appearance

The white-bodied larvae of L. coeruleiviridis develop in three stages, called instars. In each instar, the larva grows larger and larger. Its only function in this stage is eating until the final growth stage to adult fly. The marked differences between each instar are seen in the spiracles of the maggot on the posterior end. During the first instar, the larva has “Y-V” shaped spiracles. The second instar can be characterized by the shape of the spiracles increasing in size as well as number in that the “Y-V” orientation becomes 2 distinct slits on each side. Likewise, the third instar larva has 3 larger spiracular slits on each side. [3] It has also been seen that the larval stages of Lucilia coeruleiviridis are very similar to those of Lucilia eximia , though no sufficient data has been collected. [4]

Distribution and habitat

Lucilia coeruleiviridis has a Nearctic distribution, which means; of, relating to, or being the biogeographic subregion that includes Greenland and North America north of Tropical Mexico and is very ubiquitous in the southern United States. This particular blowfly is probably even the most preponderate of all species of blowflies in the southeastern United States during the spring and fall and will remain active during mild winters. [1]

Life cycle and behavior

Life cycle

Typically the blowfly, such as Lucilia coeruleiviridis, will deposit their eggs by way of the female's abdomen, which extends to form an ovipositor, in areas around accessible natural body openings such as eyes, nostrils, ears, mouth, anus and genitals or near wounds. [5] The reason that these maggot mass formations are important is because it can indicate premortem or perimortem trauma. [1] The life cycle of Lucilia coeruleiviridis has four stages of development. Calliphorids are necrophagous so the eggs are dispatched on rotting animal remains and generally hatch after roughly twelve hours. The larvae will then accumulate and nourish on the decomposing carcass. They will then undergo three larval stages (instars), which on average will take eleven to twenty days, if the ambient temperature is eighty degrees Fahrenheit. In the fourth stage, the larvae leave the food source and will pupate. The pupal stage can last from six to twelve days. A single female fly can lay in upwards of two thousand eggs in its life.

Behavior

Many of the species of major connotation are found in three families; Calliphoridae (blow flies), which includes Lucilia coeruleiviridis, Muscidae (house flies) and Sarcophagidae (flesh flies). Some species of Calliphoridae and Sarcophagidae are known to be parasitic, however, the prevailing rule for carrion feeding species is scavenging and such is true with Lucilia coeruleiviridis. [6] Lucilia coeruleiviridis is a warm weather fly whose perfect temperature is between 75 and 85 degrees Fahrenheit (23-29 degrees Celsius). The females of this species are heavily attracted to flesh and are potential mechanical vectors. [7]

Importance

Medical importance

It has been found that the maggots of the green bottle fly prefer necrotic tissue and will leave living tissue alone, so they are often used in maggot therapy, or Maggot Debridement Therapy (MDT).[ citation needed ] This therapy is the intentional introduction of disinfected maggots raised to clean out wounds that will not heal, typically larger wounds. However, Lucilia sericata — the common green bottle fly— is the preferred species. The maggots have three primary duties: to clean out wounds by eating dead tissues, kill off the bacteria, and encourage healthy tissue growth.[ citation needed ]

Forensic importance

Blow flies are generally the first to arrive on a carcass and Lucilia coeruleiviridis is no exception. Because of this, and as with all flies of the family Calliphoridae, these flies are important for time of death estimations. The larvae are also the most abundant third-instar calliphorids that are found on a carcass. Unfortunately, not a lot of study has been done on the life cycle of Lucilia coeruleiviridis due to the fact that rearing of larvae has been largely unsuccessful. Therefore, the PMI (post mortem interval) for this species is still unknown, despite being an important PMI indicator species. [3] Some Calliphorids of forensic importance, often associated with L. coeruleiviridis, include Cochliomyia macellaria , Chrysomya rufifacies , Phormia regina , Chrysomya megacephala , and Calliphora vicina . Other important Calliphorids are Calliphora vomitoria , Calliphora livida , Lucilia cuprina , Lucilia sericata, and Lucilia illustris . [7]

Cultural importance

There is a fable that says Lucilia species can predict death and show up before it occurs. [7] This idea may have originated much in the same way the theory of Spontaneous Generation came about around two millennia ago, based on how Calliphorids are typically the first insects to arrive on a carcass. The theory was a supposed process that life would come from sources other than seeds or parents. Understanding this makes understanding how this cultural fable may have come about much easier.

Research

Much research has been conducted using Lucilia coeruleiviridis and other Diptera of the family Calliphoridae. L. coeruleiviridis is particularly important in the use of forensic entomology (the relationship between the study of arthropods and the legal system) and more specifically medico-criminal entomology, which usually deals with death and decomposition of carrion. The behavior and life cycle of L. coeruleiviridis, such as the length of time it takes to arrive to carrion, lay eggs, and for those larvae to grow is used to aid scientists in determining the time elapsed after the death of a body. [8] Because the time of colonization and reproduction of different types of blowflies is so important, and species-specific, scientists are looking for techniques to more efficiently distinguish between similar looking larvae of various species. One potential plan is to develop an antigen –based diagnostic test that uses Mitochondrial DNA. This type of DNA is preferred because of its “high copy number, ease of isolation, and conserved sequence across taxa with supposed high mutation regions making discrimination between species, and even subspecies possible.” [9] Using lateral flow technology, an identified species-specific antigen is tested to determine whether the fly larva belongs to the predicted species.

Related Research Articles

Forensic entomology

Forensic entomology is the scientific study of the invasion of the succession pattern of arthropods with their developmental stages of different species found on the decomposed cadavers during legal investigations. It is the application and study of insect and other arthropod biology to criminal matters. It also involves the application of the study of arthropods, including insects, arachnids, centipedes, millipedes, and crustaceans to criminal or legal cases. It is primarily associated with death investigations; however, it may also be used to detect drugs and poisons, determine the location of an incident, and find the presence and time of the infliction of wounds. Forensic entomology can be divided into three subfields: urban, stored-product and medico-legal/medico-criminal entomology.

Maggot Larva of a fly

A maggot is the larva of a fly ; it is applied in particular to the larvae of Brachycera flies, such as houseflies, cheese flies, and blowflies, rather than larvae of the Nematocera, such as mosquitoes and Crane flies. A 2012 study estimated the population of maggots in North America alone to be in excess of 3×1017.

Calliphoridae Family of insects in the Diptera order

The Calliphoridae are a family of insects in the order Diptera, with 1,200 known species. The maggot larvae, often used as fishing bait, are known as gentles. The family is known to be polyphyletic, but much remains disputed regarding proper treatment of the constituent taxa, some of which are occasionally accorded family status.

Common green bottle fly Species of insect

The common green bottle fly is a blowfly found in most areas of the world and is the most well-known of the numerous green bottle fly species. Its body is 10–14 millimetres (0.39–0.55 in) in length – slightly larger than a house fly – and has brilliant, metallic, blue-green or golden coloration with black markings. It has short, sparse black bristles (setae) and three cross-grooves on the thorax. The wings are clear with light brown veins, and the legs and antennae are black. The larvae of the fly may be used for maggot therapy, are commonly used in forensic entomology, and can be the cause of myiasis in livestock and pets.

<i>Cynomya mortuorum</i> Species of fly

Cynomya mortuorum belongs to the order Diptera, sometimes referred to as "true flies". In English, the only common name occasionally used is "fly of the dead". It has a bluish-green appearance, similar to other Calliphoridae and is found in multiple geographic locations with a preference for colder regions. Belonging to the family Calliphoridae, it has been shown to have forensically relevant implications due to its appearance on carrion. Current research is being done to determine C. mortuorum's level of importance and usage within forensic entomology.

<i>Calliphora vomitoria</i> Species of fly

Calliphora vomitoria, known as the blue bottle fly, orange-bearded blue bottle, or bottlebee is a species of blow fly, a species in the family Calliphoridae. Calliphora vomitoria is the type species of the genus Calliphora. It is common throughout many continents including Europe, Americas, and Africa. They are fairly large flies, nearly twice the size of the housefly. They can be easily identified by their shiny, blue bodies.

<i>Chrysomya</i> Genus of flies

Chrysomya is an Old World blow fly genus of the family Calliphoridae. The genus Chrysomya contains a number of species including Chrysomya rufifacies and Chrysomya megacephala. The term “Old World blow fly” is a derivative of both the associated family, Calliphoridae, and the belief that the genus Chrysomya originated in Asia and migrated to North America only relatively recently. Chrysomya’s primary importance to the field of medico-criminal forensic entomology is due to the genus’ reliable life cycle, allowing investigators to accurately develop a postmortem interval. Chrysomya adults are typically metallic colored with thick setae on the meron and plumose arista. The name comes from the word chrysos, meaning “golden” in reference to the metallic sheen of the genus’ species, and -mya, a derivation from the word myia, meaning “fly”.

<i>Chrysomya rufifacies</i> Species of blow fly

Chrysomya rufifacies is a species belonging to the blow fly family, Calliphoridae, and is most significant in the field of forensic entomology due to its use in establishing or altering post mortem intervals. The common name for the species is the hairy maggot blow fly, and it belongs to the genus Chrysomya, which is commonly referred to as the Old World screwworms. This genus includes other species such as Chrysomya putoria and Chrysomya bezziana, which are agents of myiasis. C. rufifacies prefers very warm weather and has a relatively short lifecycle. It is widely distributed geographically and prefers to colonize large carcasses over small ones. The species commonly has a greenish metallic appearance and is important medically, economically, and forensically.

Medicolegal entomology is a branch of forensic entomology that applies the study of insects to criminal investigations, and is commonly used in death investigations for estimating the post-mortem interval (PMI). One method of obtaining this estimate uses the time and pattern of arthropod colonization. This method will provide an estimation of the period of insect activity, which may or may not correlate exactly with the time of death. While insect successional data may not provide as accurate an estimate during the early stages of decomposition as developmental data, it is applicable for later decompositional stages and can be accurate for periods up to a few years.

<i>Lucilia illustris</i> Species of insect

Lucilia illustris is a member of the fly family Calliphoridae, commonly known as a blow fly. Along with several other species, L. illustris is commonly referred to as a green bottle fly. L. illustris is typically 6–9 mm in length and has a metallic blue-green thorax. The larvae develop in three instars, each with unique developmental properties. The adult fly typically will feed on flowers, but the females need some sort of carrion protein in order to breed and lay eggs.

<i>Calliphora vicina</i> Species of insect

Calliphora vicina is a member of the family Calliphoridae, which includes blow flies and bottle flies. These flies are important in the field of forensic entomology, being used to estimate the time of a person's death when a corpse is found and then examined. C. vicina is currently one of the most entomologically important fly species for this purpose because it arrives at and colonizes a body following death in consistent timeframes.

<i>Phormia regina</i> Species of fly

The species Phormia regina , more commonly known as the black blow fly, belongs to the blow fly family Calliphoridae and was first described by Johann Wilhelm Meigen.

<i>Chrysomya albiceps</i> Species of fly

Chrysomya albiceps is a species belonging to the blow fly family, Calliphoridae.

Lucilia thatuna belongs to the family Calliphoridae, the species most commonly referred to as the blowflies, and the genus Lucilia. Along with several other species of Lucilia, L. thatuna is commonly referred to as a green bottle fly. L. thatuna is very scarce and not much is known about this particular fly. It has been noted to reside in mountainous regions of the northwestern United States.

<i>Cynomya cadaverina</i> Species of fly

Cynomya cadaverina, also known as the shiny blue bottle fly, is a member of the family Calliphoridae, which includes blow flies as well as bottle flies. In recent years, this family has become a forensically important facet in many medicocriminal investigations in the growing field of forensic entomology. C. cadaverina is specifically important in determining a post-mortem interval, as well as other important factors.

<i>Lucilia mexicana</i> Species of insect

Lucilia mexicana is a species of blow fly of the family Calliphoridae, one of many species known as a green bottle fly. Its habitat range extends from southwestern North America to Brazil. L. mexicana is typically 6–9 mm in length with metallic blue-green coloring. This species is very similar in appearance to L. coeruleiviridis, the primary difference being that L. mexicana has two or more complete rows of post-ocular setae. L. mexicana has the potential to be forensically important in the stored-products and medicocriminal fields, but more research is needed for the fly to be used as evidence in criminal investigations.

<i>Calliphora livida</i>

Calliphora livida is a member of the family Calliphoridae, the blow flies. This large family includes the genus Calliphora, the "blue bottle flies". This genus is important in the field of forensic entomology because of its value in post-mortem interval estimation.

<i>Protophormia terraenovae</i> Species of fly

Protophormia terraenovae is commonly called northern blowfly, blue-bottle fly or blue-assed fly. It is distinguished by its deep blue coloration and large size and is an important species throughout the northern hemisphere. This fly is notable for its economic effect as a myiasis pest of livestock and its antibiotic benefits in maggot therapy. Also of interest is P. terraenovae’s importance in forensic investigations: because of their temperature-dependent development and their prominent presence on corpses, the larvae of this species are useful in minimum post-mortem interval (mPMI) determination.

Calliphora latifrons is a species of blue bottle fly.

Calliphora loewi is part of the family Calliphoridae, bottle flies and blowflies, and in the genus Calliphora, blue bottle flies. The genus can be deceiving since C. loewi is not blue. Though this species is rare, it can play an important part in forensic entomology, spreading disease, and decomposing carrion. The life cycle of C. loewi is similar to the life cycle of the genus Calliphora. Since this species is rare there has not been very much research done with this species.

References

  1. 1 2 3 Byrd, J.H., & Castner, J.L. (Eds.). (2001). Forensic Entomology: The Utility of Arthropods in Legal Investigations. Florida: CRC Press
  2. Brundage, Adrienne, “Calliphoridae Continued”. Texas A&M University, College Station. 23 Feb. 2009
  3. 1 2 Gruner, Susan V.; Slone, Daniel H.; Capinera, John L.; “The Forensically Important Calliphoridae (Insecta: Diptera) of Pig Carrion in Rural North-Central Florida”; Journal of Medical Entomology 44 (3): pp. 509-515. Retrieved on 2008-03-18
  4. Kirkpatrick, Ryan Scott (August 2004). “Nocturnal light and temperature influences on necrophagous, carrion-associating blow fly species (Diptera: Calliphoridae) of forensic importance in Central Texas.” Master's thesis, Texas A&M University.
  5. Elmer Catts, Jr, & Neal H. Haskell. Entomology and Death: a Procedural Guide. Mahwah: Forensic Entomology Associates, 1990.
  6. Catts>Elmer Catts, Jr, & Neal H. Haskell. Entomology & Death: A Procedural Guide. Mahwah: Forensic Entomology Associates, 1990
  7. 1 2 3 Brundage Adrienne, Calliphoridae." Texas A&M University, College Station. 8 Feb.2008.
  8. Hall, Robert D., and Kathy E. Doisy. "Length of Time After Death: Effect on Attraction and Oviposition or Larviposition of Midsummer Blow Flies (Diptera: Calliphoridae) and Flesh Flies (Diptera: Sarcophagida) of Medicolegal Importance in Missouri." Annals of the Entomological Society of America 1993 (1993): 589-93.
  9. L. McDonagh, et al., Development of an antigen-based rapid diagnostic test for the identification of blowfly (Calliphoridae) species of forensic significance, Forensic Sci. In. Genet. (2009), doi10.1016/j.fsigen.2009.01.013