MKI67IP

NIFK
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 2AFF
Identifiers
Aliases	NIFK , MKI67IP, Nopp34, nucleolar protein interacting with the FHA domain of MKI67
External IDs	OMIM: 611970 MGI: 1915199 HomoloGene: 49862 GeneCards: NIFK
Gene location (Human) Chr. Chromosome 2 (human) [1] Band 2q14.3 Start 121,726,945 bp [1] End 121,736,911 bp [1]
Gene location (Mouse) Chr. Chromosome 1 (mouse) [2] Band 1|1 E2.3 Start 118,249,569 bp [2] End 118,261,552 bp [2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in Achilles tendon ganglionic eminence tibialis anterior muscle body of pancreas lymph node endometrium appendix islet of Langerhans pericardium smooth muscle tissue Top expressed in primitive streak morula yolk sac somite abdominal wall maxillary prominence otic placode neural tube hair follicle barrel cortex More reference expression data BioGPS More reference expression data
Gene ontology Molecular function protein binding RNA binding nucleic acid binding Cellular component cytoplasm condensed nuclear chromosome chromosome nucleolus nucleus nucleoplasm Biological process negative regulation of phosphatase activity rRNA transcription rRNA metabolic process protein-containing complex assembly Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 84365 67949 Ensembl ENSG00000155438 ENSMUSG00000026377 UniProt Q9BYG3 Q91VE6 RefSeq (mRNA) NM_032390 NM_026472 RefSeq (protein) NP_115766 NP_080748 Location (UCSC) Chr 2: 121.73 – 121.74 Mb Chr 1: 118.25 – 118.26 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

FHA Ki67 binding domain of hNIFK
the solution structure of the ki67fha/hnifk(226-269)3p complex
Identifiers
Symbol	hNIFK_binding
Pfam	PF12196
InterPro	IPR021043
Available protein structures: Pfam   structures / ECOD   PDB RCSB PDB; PDBe; PDBj PDBsum structure summary

MKI67 FHA domain-interacting nucleolar phosphoprotein is a protein that in humans is encoded by the MKI67IP gene. ^{[5] [6]}

MKI67 FHA domain-interacting nucleolar phosphoprotein contains an RNA recognition motif (RRM) near to the N-terminus and a FHA Ki67 binding domain near to the C-terminus. There are two conserved sequence motifs within the FHA Ki67 binding domain: TPVCTP and LERRKS, this domain binds to the forkhead-associated domain of human Ki67. High-affinity binding requires sequential phosphorylation by two kinases, CDK1 and GSK3, yielding pThr238, pThr234 and pSer230. This interaction is involved in cell cycle regulation. ^[7]

References

1. GRCh38: Ensembl release 89: ENSG00000155438 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000026377 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Takagi M, Sueishi M, Saiwaki T, Kametaka A, Yoneda Y (Jul 2001). "A novel nucleolar protein, NIFK, interacts with the forkhead associated domain of Ki-67 antigen in mitosis". J Biol Chem. 276 (27): 25386–91. doi: 10.1074/jbc.M102227200 . PMID   11342549.
6. "Entrez Gene: MKI67IP MKI67 (FHA domain) interacting nucleolar phosphoprotein".
7. Byeon IJ, Li H, Song H, Gronenborn AM, Tsai MD (November 2005). "Sequential phosphorylation and multisite interactions characterize specific target recognition by the FHA domain of Ki67". Nat. Struct. Mol. Biol. 12 (11): 987–93. doi:10.1038/nsmb1008. PMID   16244663. S2CID   5680428.

Further reading

• Scherl A, Couté Y, Déon C, et al. (2003). "Functional proteomic analysis of human nucleolus". Mol. Biol. Cell. 13 (11): 4100–9. doi:10.1091/mbc.E02-05-0271. PMC   133617 . PMID   12429849.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi: 10.1073/pnas.242603899 . PMC   139241 . PMID   12477932.
• Li H, Byeon IJ, Ju Y, Tsai MD (2004). "Structure of human Ki67 FHA domain and its binding to a phosphoprotein fragment from hNIFK reveal unique recognition sites and new views to the structural basis of FHA domain functions". J. Mol. Biol. 335 (1): 371–81. doi:10.1016/j.jmb.2003.10.032. PMID   14659764.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC   528928 . PMID   15489334.
• Andersen JS, Lam YW, Leung AK, et al. (2005). "Nucleolar proteome dynamics". Nature. 433 (7021): 77–83. Bibcode:2005Natur.433...77A. doi:10.1038/nature03207. PMID   15635413. S2CID   4344740.
• Hillier LW, Graves TA, Fulton RS, et al. (2005). "Generation and annotation of the DNA sequences of human chromosomes 2 and 4". Nature. 434 (7034): 724–31. Bibcode:2005Natur.434..724H. doi: 10.1038/nature03466 . PMID   15815621.
• Byeon IJ, Li H, Song H, et al. (2006). "Sequential phosphorylation and multisite interactions characterize specific target recognition by the FHA domain of Ki67". Nat. Struct. Mol. Biol. 12 (11): 987–93. doi:10.1038/nsmb1008. PMID   16244663. S2CID   5680428.
• Nousiainen M, Silljé HH, Sauer G, et al. (2006). "Phosphoproteome analysis of the human mitotic spindle". Proc. Natl. Acad. Sci. U.S.A. 103 (14): 5391–6. Bibcode:2006PNAS..103.5391N. doi: 10.1073/pnas.0507066103 . PMC   1459365 . PMID   16565220.

External links

• PDBe-KB provides an overview of all the structure information available in the PDB for Human MKI67 FHA domain-interacting nucleolar phosphoprotein (MKI67IP)

This article incorporates text from the public domain Pfam and InterPro: IPR021043