MOPS

Last updated
MOPS
Mops is.svg
Names
Preferred IUPAC name
3-(Morpholin-4-yl)propane-1-sulfonic acid
Other names
3-(N-Morpholino)propanesulfonic acid,
3-Morpholinopropanesulfonic acid,
3-N-Morpholino propansulfonic acid,
4-Morpholinepropanesulfonic acid
Identifiers
3D model (JSmol)
ChemSpider
ECHA InfoCard 100.013.162 OOjs UI icon edit-ltr-progressive.svg
PubChem CID
UNII
  • InChI=1S/C7H15NO4S/c9-13(10,11)7-1-2-8-3-5-12-6-4-8/h1-7H2,(H,9,10,11) X mark.svgN
    Key: DVLFYONBTKHTER-UHFFFAOYSA-N X mark.svgN
  • InChI=1/C7H15NO4S/c9-13(10,11)7-1-2-8-3-5-12-6-4-8/h1-7H2,(H,9,10,11)
    Key: DVLFYONBTKHTER-UHFFFAOYAN
  • C1COCCN1CCCS(=O)(=O)O
Properties
C7H15NO4S
Molar mass 209.26 g·mol−1
Hazards
GHS labelling:
GHS-pictogram-exclam.svg
Warning
H315, H319, H335
P261, P264, P271, P280, P302+P352, P304+P340, P305+P351+P338, P312, P321, P332+P313, P337+P313, P362, P403+P233, P405, P501
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
X mark.svgN  verify  (what is  Yes check.svgYX mark.svgN ?)

MOPS (3-(N-morpholino)propanesulfonic acid) is a buffer introduced in the 1960s, one of the twenty Good's buffers. It is a structural analog to MES, [1] and like MES, its structure contains a morpholine ring. HEPES is a similar pH buffering compound that contains a piperazine ring. With a pKa of 7.20, MOPS is an excellent buffer for many biological systems at near-neutral pH.

Contents

Applications

MOPS is frequently used as a buffering agent in biology and biochemistry. It has been tested and recommended for polyacrylamide gel electrophoresis. [2] Usage above 20 mM in mammalian cell culture work is not recommended. [3] MOPS buffer solutions become discolored (yellow) over time, but reportedly slight discoloration does not significantly affect the buffering characteristics. [4]

See also

Related Research Articles

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References

  1. Good, Norman E.; Winget, G. Douglas; Winter, Wilhelmina; Connolly, Thomas N.; Izawa, Seikichi; Singh, Raizada M. M. (1966). "Hydrogen Ion Buffers for Biological Research". Biochemistry. 5 (2): 467–77. doi:10.1021/bi00866a011. PMID   5942950.
  2. Thomas, J; Hodes, ME (1981). "A new discontinuous buffer system for the electrophoresis of cationic proteins at near-neutral pH". Analytical Biochemistry. 118 (1): 194–6. doi:10.1016/0003-2697(81)90178-0. PMID   6278979.
  3. Eagle, H. (1971). "Buffer Combinations for Mammalian Cell Culture". Science. 174 (4008): 500–3. Bibcode:1971Sci...174..500E. doi:10.1126/science.174.4008.500. PMID   5110427. S2CID   29876583.
  4. "Boston BioProducts - MOPS Buffer (1 M, pH 7.4)".