Melipona quadrifasciata

Last updated

Melipona quadrifasciata
Abelha sem ferao Mandacaia.jpg
Scientific classification OOjs UI icon edit-ltr.svg
Domain: Eukaryota
Kingdom: Animalia
Phylum: Arthropoda
Class: Insecta
Order: Hymenoptera
Family: Apidae
Genus: Melipona
Species:
M. quadrifasciata
Binomial name
Melipona quadrifasciata
M. quadrifasciata.pdf
Range of M. quadrifasciata [2]

Melipona quadrifasciata is a species of eusocial, stingless bee of the order Hymenoptera. It is native to the southeastern coastal states of Brazil, where it is more commonly known as mandaçaia , which means "beautiful guard," as there is always a bee at the narrow entrance of the nest. M. quadrifasciata constructs mud hives in the hollows of trees to create thin passages that only allow one bee to pass at a time. Because they are stingless bees, M. quadrifasciata is often used as pollinators in greenhouses, outperforming honey bees in efficiency and leading to overall larger yields of fruits that were heavier, larger, and contained more seeds. [3]

Contents

Taxonomy

Melipona quadrifasciata is a member of the family Apidae and the order Hymenoptera. M. quadrifasciata is in the subfamily Meliponini which is commonly referred to as "stingless bees". The genus Melipona includes nearly 50 other species. [4] M. quadrifasciata can be categorized into two subspecies: M. quadrifasciata quadrifasciata which occupies the northern range of the species and M. quadrifasciata anthidioides which are more often found in the southern range. However, there is a large hybrid area where the subspecies overlap.

Identification and differentiation

Melipona quadrifasciata have dark black, rounded bodies with slightly curved antennae and translucent wings. Size is from 10 to 11 mm, and they are more heavily build than the common honeybee. This bee can be identified by the bright yellow stripe pattern from the third to the sixth abdominal tergites. [5] Melipona that produce workers, males, and potential queens are indistinguishable and intermixed, making caste differentiation both environmentally and genetically determined. [6]

Queens

The abdomens of M. quadrifasaciata queens swell with ovarian development, making older queens larger than workers which is typical of most social bees. Queens vary slightly in their coloring, having brown eyes and brown hair compared to the black eyes and hair of worker bees. [7]

Workers

Workers are smaller than the queen. Workers have black eyes and black hair on their thorax and abdomen. Older workers will go out foraging while younger workers, 12–21 days old, will construct and provision cells in the comb. [7]

Distribution and habitat

Melipona quadrifasciata is one of the most common Melipona species in southeastern coastal Brazil, found from the states of Pernambuco to Rio Grande do Sul. This species shows considerable variety in nesting sites and has been recorded nesting in tree trunks from 1 to 3 meters above the soil surface, in the soil in nests of Atta, and in the abandoned mud bird nests constructed on telephone poles. [8] Nests are commonly found in tree holes and incorporate clay. The opening of the nests allow for only one bee to pass through at a time. [8]

Colony cycle

Nest entrance guard Melipona quadrifasciata nest guard.jpg
Nest entrance guard

Stingless bees, such as M. quadrifasciata, are highly eusocial bees that are characterized by having perennial colonies that are typically headed by a single-mated queen. The average number of adult workers and queens within a colony is 300–400. [9] There has been a novel case of temporal polygyny within a colony of M. quadrifasciata, where eight egg-laying queens were found to be coexisting in a single colony. [9]

New colonies are established in a slow process, when the number of worker bees exceed 500 or 600 individuals in the parent colony. Then, a number of worker bees starts to build a new nest in a tree cavity found to be well suited for this purpose, and store honey and pollen in there. When the new nest is ready, a "princess bee" (mated gyne) join the workers, and if accepted it starts laying eggs and becomes the new queen. As in other Melipona bees, after a while the abdomen of the new queen expands to 3 or more times the initial size (a phenomenon called physogastrism) and it becomes incapable of flying, never leaving the nest again.

Reproduction

This section contains information on cell construction, ovipositioning, and caste differentiation of M. quadrifasciata.

Cell construction

Young worker bees of the same age group are tasked with cell construction and provisioning of brood cells. An individual can participate in both cell building and provisioning. Each cell is not built exclusively and continuously by a single worker, but by successive activities by multiple workers. Cells are built in a concentric pattern and are finished successively, not synchronously, so at any given time there is diversity in each growing stage. [7]

Worker oviposition

When a cell has been completed, the queen will fixate on the cell for inspection and push away workers from the cell. A worker will provision food into the cell and quickly retreat. Sometimes, when the queen is not around, a worker will lay an egg in the cell and quickly retreat from the area similar to when provisioning cells. During worker oviposition, or egg laying, the worker will be quiet with her wings closed and keep her body completely still as not to attract the queen. Worker oviposition will last an average of 7 seconds. [7] A worker's egg is about 23 the size of eggs laid by the queen. [7]

Queen oviposition

The queen briefly inspects the cell before inserting her metasoma. Usually, if a worker egg is found, it is eaten by the queen. The duration of queen oviposition is notably longer than worker oviposition, lasting an average of 24.5 seconds. [7] After the queen has deposited an egg, there is usually a slight delay in M. quadrifasciata workers before operculation, or closing of the cell, occurs.

Caste differentiation

It has been found in Melipona quadrifasciata that caste determination is both genetic and environmental. Melipona cells which produce workers (and males) and those which produce gynes (young queens) are indistinguishable and intermixed. Upon emergence, gynes are around the same size as workers, although different structurally. Cells that receive small amounts of provisions produce workers, while cells with larger amounts produce both gynes and workers (KERR 1966). In research done by Kerr, Stort, and Montenegro (1966) it was found that no gynes occurred among pupae weighing less than 72 mg, while above that weight around 25% of pupae were gynes. When all pupae are above 72 mg, a 3:1 segregation of workers to gynes is observed. In times when food is not adequate and pupae are of lower rate, fewer gynes develop. [6]

The 3:1 ratio of workers to gynes provides evidence of genetic caste determination. To be a queen, a female must be heterozygous at two loci. Homozygosity at either or both loci produces an individual that becomes a worker. A potential queen, having double heterozygosity, can only become a queen if environmental conditions are good and ideal pupae weight can be achieved. A doubly heterozygous offspring will become a worker if food conditions are not favorable. [6]

Queen supersedure

Potential queens, or gynes, are continually produced due to the genetic and trophic nature of caste differentiation. This provides a failsafe if a new queen is ever needed, however there must also be a device in place to dispose of the excess gynes. It is usually workers that kill excess gynes in Melipona species. However, at a time of queen supersedure in M. quadfrifasciata, several gynes will be killed before a newly accepted virgin queen helps the workers to deal with other virgin gynes that may emerge. After the newly accepted queen is established and the competition is disposed of, the new queen will take her mating flight. [8]

Development

The full period of development for Melipona quadrifasciata is approximately 38 days; 5 days of embryonic development, 15-day larval stage, and 18-day pupal stage. [10]

Communication

Melipona quadrifasciata is a highly eusocial bee, making communication imperative for the survival of the colony.

Forager recruitment

Drinking Melipona quadrifasciata drinking.jpg
Drinking

Forager recruitment in stingless bees such as M. quadrifasciata is not as acute as in honey bees. In a series of experiments done at by the University of Vienna, it was confirmed that M. quadrifasciata were able to recruit other foragers within a colony and communicate the direction but not the distance of a foraging site. In comparison with other species of bee, M. quadrifasciata was not as great at communicating the location of a foraging site, but this could be due to the abundant and easily encountered food sources in its habitat in the forests of Brazil. [11]

Similar to Melipona bicolor , M. quadrifasciata are mostly active outside of their nest in the morning, when humidity was high and light intensity and temperature were moderate. They forage for food in the first few hours of the morning. [12]

Relation to humans

Melipona quadrifasciata is commonly used in agriculture in South America, but wild bees are feeling the effects of deforestation and pesticides.

Melipona quadrifasciata hive Mandacaias.jpg
Melipona quadrifasciata hive

Greenhouse pollinators

The ancient Maya domesticated a separate species of stingless bee. The use of stingless bees is referred to as meliponiculture, named after bees of the tribe Meliponini—such as Melipona quadrifasciata in Brazil. This variation of bee keeping still occurs around the world today. [13] M. quadrifasciata is frequently harvested to be used as a greenhouse pollinator because it is stingless and can easily live in man-made hives. M. quadrifasciata outperformed honey bees as greenhouse pollinators to yield fruits that were larger and carried more seeds than those pollinated by honey bees. [3] The nests of M. quadrifasciata and other stingless bees of the Meliponini tribe are commonly used to harvest honey in Central and South America.

Beekeeping

This species is well suited for rational beekeeping, as colonies grant introduction of new queens from other areas, allowing for exchange of queens between beekeepers in different regions of Brazil. Honey from M. quadrifasciata is produced sporadically in the Brazilian state of Bahia as an additional source of income. The honey is typically collected and sold by women, and in a good blooming season a single hive can produce 1 to 1.5 liters of honey. In warm regions honey is produced the whole year round, and production can be considerably higher. M. quadrifasciata honey is used both for consumption and medicinal purposes.

These bees are exceedingly tame, never attacking humans, even to defend their nest. Their normal reaction when the hive is opened is to hide in dark corners, usually they do not attempt to fly away. In strong colonies, a few workers may fly around the intruder, but will avoid touching it. This behavior greatly facilitates the work of beekeepers interested in raising them. [14]

Deforestation

Cropland expansion and logging in the Brazilian cerrado has reduced the abundance of Meliponini bees. The fragmentation of habitat affects floral food sources and nesting sites. M. quadrifasciata seeks nesting sites in hollows of trees located a few meters up from the ground, creating a rather narrow niche. Only one species of tree in an area of the Brazilian cerrado is federally protected, Caryocar brasiliense , which is widely used by M. quadrifasciata for nesting. [15]

Pesticide usage

Biopesticides are perceived as environmentally safe due to their natural origins. However, recent studies have shown lethal and sublethal risks to local pollinators as unintended consequences of biopesticide usage. M. quadrifasciata is an important native pollinator in Brazil, and was therefore used to study the effects of biopesticides on pollinators. It was found that the pesticides spinosad and imidacloprid—commonly used against crop pests—greatly impaired worker respiration, group activity, and flight in M. quadrifasciata. [16]

Related Research Articles

<span class="mw-page-title-main">Stingless bee</span> Bee tribe, reduced stingers, strong bites

Stingless bees, sometimes called stingless honey bees or simply meliponines, are a large group of bees, comprising the tribe Meliponini. They belong in the family Apidae, and are closely related to common honey bees, carpenter bees, orchid bees, and bumblebees. Meliponines have stingers, but they are highly reduced and cannot be used for defense, though these bees exhibit other defensive behaviors and mechanisms. Meliponines are not the only type of bee incapable of stinging: all male bees and many female bees of several other families, such as Andrenidae, also cannot sting. Some stingless bees have powerful mandibles and can inflict painful bites.

<i>Schwarziana quadripunctata</i> Species of bee

Schwarziana quadripunctata is a small, stingless bee found in a stretch of the South American Amazon from Goiás, Brazil, through Paraguay, to Misiones, Argentina. This highly eusocial insect constructs earthen nests in the subterranean level of the subtropical environment, an unusual feature among other stingless bees. The species ranges in sizes from 6.0 to 7.5 millimetres and feeds on a diverse diet of flowering plants found abundantly on the forest floor, including guacatonga and the mistletoe species Struthanthus concinnus.

<i>Tetragonula carbonaria</i> Species of bee

Tetragonula carbonaria is a stingless bee, endemic to the north-east coast of Australia. Its common name is sugarbag bee. They are also occasionally referred to as bush bees. The bee is known to pollinate orchid species, such as Dendrobium lichenastrum, D. toressae, and D. speciosum. It has been identified as an insect that collects pollen from the cycad Cycas media. They are also known for their small body size, reduced wing venation, and highly developed social structure comparable to honey bees.

<i>Trigona spinipes</i> Species of bee

Trigona spinipes is a species of stingless bee. It occurs in Brazil, where it is called arapuá, aripuá, irapuá, japurá or abelha-cachorro ("dog-bee"). The species name means "spiny feet" in Latin. Trigona spinipes builds its nest on trees, out of mud, resin, wax, and assorted debris, including dung. Therefore, its honey is not fit for consumption, even though it is reputed to be of good quality by itself, and is used in folk medicine. Colonies may have from 5,000 to over 100,000 workers.

<i>Tetragonisca angustula</i> Species of bee

Tetragonisca angustula is a small eusocial stingless bee found in México, Central and South America. It is known by a variety of names in different regions. A subspecies, Tetragonisca angustula fiebrigi, occupies different areas in South America and has a slightly different coloration.

<i>Tetragonula iridipennis</i> Species of bee

The Indian stingless bee or dammar bee, Tetragonula iridipennis, is a species of bee belonging to the family Apidae, subfamily Apinae. It was first described by Frederick Smith in 1854 who found the species in what is now the island of Sri Lanka. Many older references erroneously placed this species in Melipona, an unrelated genus from the New World, and until recently it was placed in Trigona, therefore still often mistakenly referred to as Trigona iridipennis. For centuries, colonies of T. iridipennis have been kept in objects such as clay pots so that their highly prized medicinal honey can be utilized.

<i>Melipona bicolor</i> Species of bee

Melipona bicolorLepeletier, 1836, commonly known as Guaraipo or Guarupu, is a eusocial bee found primarily in South America. It is an inhabitant of the Araucaria Forest and the Atlantic Rainforest, and is most commonly found from South to East Brazil, Bolivia, Argentina, and Paraguay. It prefers to nest close to the soil, in hollowed trunks or roots of trees. M. bicolor is a member of the tribe Meliponini, and is therefore a stingless bee. This species is unique among the stingless bees species because it is polygynous, which is rare for eusocial bees.

<i>Plebeia remota</i> Species of bee

Plebeia remota is a species of stingless bee that is in the family Apidae and tribe Meliponini. Bees of the species are normally found in a few states in southern Brazil and their nests can be found in tree cavities. Depending on the region, P. remota may have a different morphology and exhibit different behaviors. The bee's diet consists of nectar and pollen that are collected intensely from a few sources. Researchers have conducted a multitude of studies analyzing the changes that occur in the colony during reproductive diapause and what happens during the provisioning and oviposition process or POP.

<i>Melipona beecheii</i> Species of bee

Melipona beecheii is a species of eusocial stingless bee. It is native to Central America from the Yucatán Peninsula in the north to Costa Rica in the south. M. beecheii was cultivated in the Yucatán Peninsula starting in the pre-Columbian era by the ancient Maya civilization. The Mayan name for M. beecheii is xunan kab, which translates roughly to "regal lady bee". M. beecheii serves as the subject of various Mayan religious ceremonies.

<i>Trigona corvina</i> Species of bee

Trigona corvina is a species of stingless bee that lives primarily in Central and South America. In Panama, they are sometimes known as zagañas. They live in protective nests high in the trees, but they can be extremely aggressive and territorial over their resources. They use their pheromones to protect their food sources and to signal their location to nest mates. This black stingless bees of the tribe Meliponini can be parasitic toward citrus trees but also helpful for crop pollination.

<i>Melipona subnitida</i> Species of bee

Melipona subnitida is a neotropical bee species in the Apidae family found in the dry areas of Northeastern Brazil. This species of stingless bees practices single mating, monogynous habits.

<i>Nannotrigona testaceicornis</i> Species of bee

Nannotrigona testaceicornis is a eusocial stingless bee species of the order Hymenoptera and the genus Nannotrigona. Its local common name is abelhas iraí. This species has a large geographic distribution and occupies different biomes, including urban areas, around Neotropical America. The bees of this species nest in trees or artificial cavities because of this broad distribution. N. testaceicornis is important for agriculture because it will pollinate a vast number of plant species year round.

<i>Scaptotrigona postica</i> Species of bee

Scaptotrigona postica is a species of stingless bee that lives mainly in Brazil. It is a eusocial bee in the tribe Meliponini. S. postica is one of 25 species in the genus Scaptotrigona and is a critical pollinator of the tropical rain forests of Brazil. They construct their nests in hollowed sections of tree trunks, allowing for effective guarding at the nest entrance. This species shows colony structure similar to most members of the Meliponini tribe with three roles within the colony: queen, worker, and male. S. postica individuals have different forms of communication from cuticular hydrocarbons to pheromones and scent trails. Communication is especially useful during worker foraging for nectar and pollen through the Brazilian tropical rain forests. S. postica is a very important pollinator of the Brazilian tropical rain forests and is widely appreciated for its honey. Stingless bees account for approximately 30% of all pollination of the Brazilian Caatinga and Pantanal ecosystems and up to 90% of the pollination for many species of the Brazilian Atlantic Forest and the Amazon.

<i>Bombus pauloensis</i> Species of bee

Bombus pauloensis is a neotropical bumblebee, formerly known as Bombus atratus, that is found throughout regions of South America, including Colombia, Ecuador, Brazil, and Argentina. It lives in social colonies that include a founder queen/queens, workers and brood. B. pauloensis is somewhat unusual because of its potential to oscillate between polygynous and monogynous nesting cycles. Bombus pauloensis was the first species in the genus Bombus that was discovered to display such polygynous nesting patterns. The polygynous nesting cycles lead to certain specific types of behavior including queen-queen aggression. Nests can also be perennial, which is a characteristic rarely found in other bumblebees. B. pauloensis can be helpful to agricultural because of their ability to pollinate different species of plants. B. pauloensis has been found to occupy a range of geographic areas and climates throughout South America. Colonies have the ability to thermoregulate nests and keep them a little bit warmer than the outside environment. Foraging workers use muscle contractions to maintain stable temperatures and coupe with seasonal and daily fluctuations in temperature.

<i>Paratrigona subnuda</i> Species of bee

Paratrigona subnuda, commonly known as the jataí-da-terra, is a species of eusocial stingless bee in the family Apidae and tribe Meliponini. These social bees are prevalent in Neotropical moist forests, including Brazilian Atlantic and other South American forests. They inhabit spherical nests in moist underground environments with their forest habitats. Within their Neotropical habitats the P. subnuda is considered to be a very successful and common species of bee. P. subnuda’s main source of food is pollen and nectar from a large variety of native Mesoamerican tropical plants. They have been extensively studied due to social conflicts arising from single mate behaviors and particular virgin behaviors. P. subnuda also exhibits the particular daily behavior in which they open the nest entrance at dawn and close the entrance at dusk when all their activities are done.

<i>Scaptotrigona mexicana</i> Species of bee

Scaptotrigona mexicana is a species of stingless bee that lives throughout Mexico and is part of the Meliponini tribe. This species is sometimes termed "Pisil Nekmej" and is extensively studied for its medicinal purposes. This species is considered common and abundant throughout Mexico and it has been noted to thrive in tropical environments.

<i>Melipona scutellaris</i> Species of bee

Melipona scutellaris is a eusocial stingless bee species of the order Hymenoptera and the genus Melipona. It is considered to be the reared Melipona species with the largest distribution in the North and Northeast regions of Brazil, with records from Rio Grande do Norte down to Bahia. Its common name, Uruçu, comes from the Tupi "eiru su", which in this indigenous language means "big bee". Their honey is highly desirable and the materials they create for nests have been proven to be a promising source of antibiofilm agents and to present selectivity against human cancer cell lines at low concentrations compared to normal cells.

<i>Trigona fuscipennis</i> Species of bee

Trigona fuscipennis is a stingless bee species that originates in Mexico but is also found in Central and South America. They are an advanced eusocial group of bees and play a key role as pollinators in wet rainforests. The species has many common names, including mapaitero, sanharó, abelha-brava, xnuk, k'uris-kab, enreda, corta-cabelo, currunchos, zagaño, and enredapelos.

<i>Lestrimelitta limao</i> Species of bee

Lestrimelitta limao is a neotropical eusocial bee species found in Brazil and Panama and is part of the Apidae family. It is a species of stingless bees that practices obligate nest robbing. They have never been spotted foraging from flowers, an observation that supports their raiding behavior. Because of their lack of hind corbiculae, they must raid to obtain enough protein in their diet in the form of pollen and nectar. Lestrimelitta limao secrete a lemon-scented alarm allomone, from which they receive their name, in order to conduct successful raids. L. limao are hypothesized to produce poisonous honey that is toxic if consumed by humans. Because robber bees are so rare and difficult to observe, there is a limited scope of information available.

<i>Frieseomelitta varia</i> Species of bee

Frieseomelitta varia, commonly known as the abelha marmelada-amarela, is a species of eusocial stingless bee in the family Apidae and tribe Meliponini. F. varia is native to Brazil.

References

  1. Nogueira, Juliano; et al. (2014). "Conservation study of an endangered stingless bee (Melipona capixaba—Hymenoptera: Apidae) with restricted distribution in Brazil". Journal of Insect Conservation. 18 (3): 317–326. doi:10.1007/s10841-014-9639-3. S2CID   16597240.
  2. Marcelo Fidelis Marques Mendes; et al. (2007). "INTRA-POPULATIONAL VARIABILITY OF Melipona tquadrifasciata Lepeletier, 1836 (Hymenoptera, Meliponini) USING RELATIVE WARP ANALYSIS". Embrapa. 23 (1): 147–152.
  3. 1 2 Bispo dos Santos, S.A.; Roselino, A.C.; Hrncir, M.; Bego, L.R. (1 January 2009). "Pollination of tomatoes by the stingless bee Melipona quadrifasciata and the honey bee Apis mellifera (Hymenoptera, Apidae)". Genetics and Molecular Research. 8 (2): 751–757. doi: 10.4238/vol8-2kerr015 . PMID   19681026.
  4. Ramírez, Santiago R.; Nieh, James C.; Quental, Tiago B.; Roubik, David W.; Imperatriz-Fonseca, Vera L.; Pierce, Naomi E. (1 August 2010). "A molecular phylogeny of the stingless bee genus Melipona (Hymenoptera: Apidae)". Molecular Phylogenetics and Evolution. 56 (2): 519–525. doi:10.1016/j.ympev.2010.04.026. PMID   20433931.
  5. Souza, Rogério O.; Moretto, Geraldo; Arias, Maria C.; Lama, Del; A, Marco (2008). "Differentiation of Melipona quadrifasciata L. (Hymenoptera, Apidae, Meliponini) subspecies using cytochrome b PCR-RFLP patterns". Genetics and Molecular Biology. 31 (2): 445–450. doi: 10.1590/S1415-47572008000300009 . ISSN   1415-4757.
  6. 1 2 3 Kerr; Nielsen (1966). "Evidences that genetically determined Melipona queens can become workers". Genetics. 54 (3): 859–866. doi:10.1093/genetics/54.3.859. PMC   1211207 . PMID   5970624.
  7. 1 2 3 4 5 6 Sakagami, Shoichi (1965). "Behavior studies of the stingless bees, with special reference to the oviposition process.:V. Melipona quadrifasciata anthidioides Lepeletier (With 7 Text-figures and 1 Table)". Journal of the Faculty of Science Hokkaido University Series VI. Zoology.
  8. 1 2 3 Michener, Charles D. (1974). The Social Behavior of the Bees: a comparative study. Belknap Press. pp. 113–114. ISBN   978-0674811751.
  9. 1 2 Alves, Denise Araujo; Menezes, Cristiano; Imperatriz-Fonseca, Vera Lucia; Wenseleers, Tom (19 May 2011). "First discovery of a rare polygyne colony in the stingless bee Melipona quadrifasciata (Apidae, Meliponini)". Apidologie. 42 (2): 211–213. doi:10.1051/apido/2010053. ISSN   0044-8435. S2CID   12896976.
  10. Rossini, AS. 1989. Caracterização das mudas ontogenéticas e biometria dos corpora allata de Melipona quadrifasciata anthidioides Lep. (Hymenoptera, Apidae). Dissertação de Mestrado, IBCR-UNESP.
  11. Stefan Jarau, Michael Hrncir, Ronaldo Zucchi, Friedrich Barth. Recruitment behavior in stingless bees, Melipona scutellaris and M. quadrifasciata. I. Foraging at food sources differing in direction and distance. Apidologie, Springer Verlag (Germany), 2000, 31 (1), pp.81–91. <10.1051/apido:2000108>.
  12. Hilario, S. D.; Imperatriz-Fonseca, V. L.; Kleinert, A. de M.P. (2000). "Flight activity and colony strength in the stingless bee Melipona bicolor bicolor (Apidae, Meliponinae)". Rev. Bras. Biol. 60 (2): 299–306. doi: 10.1590/S0034-71082000000200014 . PMID   10959114.
  13. Quezada-Euán, José Javier G.; May-Itzá, William de Jesús; González-Acereto, Jorge A. (1 January 2001). "Meliponiculture in Mexico: problems and perspective for development". Bee World. 82 (4): 160–167. doi:10.1080/0005772X.2001.11099523. ISSN   0005-772X. S2CID   85263563.
  14. Aidar, Davi S.; Campos, Lucio A. O. (1998). "Manejo e manipulação artificial de colônias de Melipona quadrifasciata Lep. (Apidae: Meliponinae)". Anais da Sociedade Entomológica do Brasil. 27 (1): 157–159. doi: 10.1590/S0301-80591998000100021 . ISSN   0301-8059.
  15. Antonini, Yasmine; Martins, Rogério P. (1 September 2003). "The value of a tree species (Caryocar brasiliense) for a stingless bee Melipona quadrifasciata quadrifasciata". Journal of Insect Conservation. 7 (3): 167–174. doi:10.1023/A:1027378306119. ISSN   1366-638X. S2CID   6080884.
  16. Tomé, Hudson Vaner V.; Barbosa, Wagner F.; Martins, Gustavo F.; Guedes, Raul Narciso C. (1 April 2015). "Spinosad in the native stingless bee Melipona quadrifasciata: Regrettable non-target toxicity of a bioinsecticide". Chemosphere. 124: 103–109. doi:10.1016/j.chemosphere.2014.11.038. PMID   25496737.