The Atlantic, Suwannee River and Gulf Railroad Company was a railroad under the control of the Florida Central and Peninsular Railroad (FC&PR) that ran westward from Starke, Florida, eventually terminating at Wannee, Florida, on the Suwannee River. It was later absorbed by the Seaboard Air Line Railroad.
The South Florida Railroad was a railroad from Sanford, Florida, to Tampa, Florida, becoming part of the Plant System in 1893 and the Atlantic Coast Line Railroad in 1902. It served as the southernmost segment of the Atlantic Coast Line's main line. The line remains in service today and is now part of the Central Florida Rail Corridor in the Orlando metro area. The rest of the line remains under the ownership of CSX Transportation as part of their A Line.
The Florida Midland Railroad Company, Inc. is one of several short line railroads operated by Regional Rail, LLC in Florida. The Florida Midland Railroad operates two former CSX Transportation railroad lines including their former Lake Wales Subdivision, and their former Bartow Subdivision. It once had a third line from Wildwood to Leesburg that is now mostly abandoned. Florida Midland Railroad began operating the lines in 1987 and uses locomotives branded for the Florida Central Railroad, its sister railroad.
The Florida Northern Railroad Company, Inc. is one of several short line railroads run by Regional Rail, LLC. It has connections to CSX at Ocala, Florida, running north to Lowell, Florida, and south to Candler, Florida. It was formerly run by CSX as their Ocala Subdivision.
The Silver Star is a passenger train operated by Amtrak on a 1,522-mile (2,449 km) route between New York City and Miami via Washington, D.C., Richmond, Raleigh, Columbia, Savannah, Jacksonville, and Tampa. The Silver Star and its partner in the Silver Service brand, the Silver Meteor, are the descendants of numerous long-distance trains that operated between Florida and New York for most of the 20th century.
The Riceboro Southern Railway began operations in 2004 operating on about 33 miles of track, some of which is leased from CSX Transportation. The track on which it operates is part of the ex-Seaboard Air Line route from Savannah, Georgia to Jacksonville, Florida. It runs generally from Ogeechee, Georgia, where the line splits from the CSX Savannah Subdivision, which is the former Atlantic Coast Line Railroad's Savannah-Jacksonville route, and Riceboro. It does not have any of its own locomotives; it uses Georgia Central power.
The Seaboard–All Florida Railway was a subsidiary of the Seaboard Air Line Railroad that oversaw two major extensions of the system in the early 1920s to southern Florida on each coast during the land boom. One line extended the Seaboard's tracks on the east coast from West Palm Beach down to Fort Lauderdale and Miami, while the other extension on the west coast extended the tracks from Fort Ogden south to Fort Myers and Naples, with branches from Fort Myers to LaBelle and Punta Rassa. These two extensions were heavily championed by Seaboard president S. Davies Warfield, and were constructed by Foley Brothers railroad contractors. Both extensions also allowed the Seaboard to better compete with the Florida East Coast Railway and the Atlantic Coast Line Railroad, who already served the lower east and west coasts of Florida respectively.
The Florida Western and Northern Railroad was a railroad line built and operated by the Seaboard Air Line Railroad running from Coleman, Florida, all the way to West Palm Beach via Auburndale and Sebring, a distance of 204 miles. Part of the line remains today from Auburndale to West Palm Beach and is now operated by CSX Transportation as their Auburndale Subdivision and Miami Subdivision.
The Auburndale Subdivision is a rail line in southern Central Florida owned and operated by CSX Transportation. It runs along CSX’s SX Line from Auburndale south to Sebring before turning southeast to Okeechobee and then toward West Palm Beach. The Auburndale Subdivision ends near North Palm Beach County General Aviation Airport, where it continues southeast to West Palm Beach as the Miami Subdivision. The line connects with CSX’s A Line in Auburndale.
The Palmetto Subdivision is a CSX Transportation rail line in the Tampa Bay region of Florida. It runs from East Tampa and roughly parallels U.S. Route 41 south through Ruskin to Palmetto and Bradenton. The Palmetto Subdivision ends just south of Tropicana Yard in Oneco, where it connects with the Seminole Gulf Railway, a shortline that continues south into Sarasota.
The Brewster Subdivision is a railroad line owned by CSX Transportation in Florida. The line runs from the Valrico Subdivision at Edison Junction south through Bradley Junction to Arcadia for a total of 47.2 miles. It junctions with the Achan Subdivision and the Agricola Spur at Bradley Junction, and connects to the Seminole Gulf Railway in Arcadia.
The Homestead Subdivision is a CSX railroad line in South Florida. Running from a junction with the South Florida Rail Corridor in Hialeah near Miami International Airport south to Homestead, the line is the southernmost railroad line in the continental United States.
CSX Transportation's Brooksville Subdivision is a railroad line in the Tampa Bay region of Florida. Today, the line runs from the Clearwater Subdivision in Sulphur Springs, Florida north to a point just north of Brooksville, a distance of 49.1 miles. Historically, the Brooksville Subdivision was owned and operated by CSX predecessor, the Seaboard Air Line Railroad. Under Seaboard ownership, the Brooksville Subdivision continued north as far as Waldo, where it intercepted the Seaboard Air Line main line.
CSX Transportation's Carters Subdivision is a segment of CSX's A Line running from Poinciana southwest to Lakeland. The Carters Subdivision ends just west of Downtown Lakeland at Lakeland Junction, where it continues west as the Lakeland Subdivision and connects to the Vitis Subdivision.
The Kingsland Subdivision is a railroad line owned by CSX Transportation in Northeast Florida. The line begins in Jacksonville at a junction with the A Line near Moncrief Yard. From there, it heads east and then turns north near Panama Park. From Panama Park, the Kingsland Subdivision heads north to Yulee on a discontinuous piece of CSX's S Line. In Yulee, the line connects with the First Coast Railroad, which operates north to Seals, Georgia, on tracks that were previously part of the Kingsland Subdivision prior to 2005.
CSX Transportation's Valrico Subdivision is a railroad line in Central Florida. It serves as CSX's main route through a region of Central Florida known as the Bone Valley, which contains the largest known deposits of phosphate in the United States.
CSX Transportation's Vitis Subdivision is a railroad line running along CSX's AR Line in Central Florida from Richland to Lakeland for a total of 19.7 miles. The Vitis Subdivision serves as a connection between CSX's two main lines in Florida, the A Line and the S Line. Its connection with the S Line at the north end is known as Vitis Junction. The connection with the A Line at the south end is known as Lakeland Junction.
The Tampa Northern Railroad was a historic railroad line running from just east of downtown Tampa north to the city of Brooksville in Hernando County. The line continues to operate today and is under the ownership of the CSX Corporation, who operates it as their Brooksville Subdivision north of Sulphur Springs and part of their Clearwater Subdivision south of there.
The Plant City, Arcadia, and Gulf Railroad was a railroad line that once operated from Plant City, Florida, south to Welcome, a distance of about 13 miles. The line remains in service today and is owned by CSX Transportation, which it operates as their Plant City Subdivision.
The Atlantic Coast Line Railroad’s Main Line was the backbone of the Atlantic Coast Line Railroad's network in the southeastern United States. The main line ran from Richmond, Virginia to Port Tampa just southwest of Tampa, Florida, a distance of nearly 900 miles. Along its route it passed through Petersburg, Rocky Mount, Florence, Charleston, Savannah, Jacksonville, and Orlando. With the exception of a short 61-mile segment in Greater Orlando, the entire line is still owned by the Atlantic Coast Line's successor, CSX Transportation, and is still in service as their A Line.
