PFKFB2

PFKFB2
Identifiers
Aliases	PFKFB2 , PFK-2/FBPase-2, 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2
External IDs	OMIM: 171835; MGI: 107815; HomoloGene: 88554; GeneCards: PFKFB2; OMA:PFKFB2 - orthologs
Gene location (Human) Chr. Chromosome 1 (human) [1] Band 1q32.1 Start 207,034,366 bp [1] End 207,081,024 bp [1]
Gene location (Mouse) Chr. Chromosome 1 (mouse) [2] Band 1|1 E4 Start 130,616,919 bp [2] End 130,656,990 bp [2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in islet of Langerhans oocyte right lobe of thyroid gland left lobe of thyroid gland gastrocnemius muscle rectum apex of heart duodenum anterior pituitary left ventricle Top expressed in neural layer of retina spermatocyte islet of Langerhans spermatid superior frontal gyrus pineal gland lumbar subsegment of spinal cord cerebellar cortex dentate gyrus of hippocampal formation granule cell primary visual cortex More reference expression data BioGPS More reference expression data
Gene ontology Molecular function transferase activity nucleotide binding kinase activity protein binding catalytic activity fructose-2,6-bisphosphate 2-phosphatase activity hydrolase activity ATP binding protein kinase binding 6-phosphofructo-2-kinase activity kinase binding Cellular component cytoplasm cytosol nucleus nucleoplasm Biological process lactate metabolic process glycolytic process phosphorylation fructose metabolic process response to glucose positive regulation of glucokinase activity fructose 2,6-bisphosphate metabolic process pyruvate metabolic process glucose catabolic process metabolism positive regulation of insulin secretion dephosphorylation carbohydrate phosphorylation positive regulation of glycolytic process Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 5208 18640 Ensembl ENSG00000123836 ENSMUSG00000026409 UniProt O60825 P70265 RefSeq (mRNA) NM_001018053 NM_006212 NM_001162415 NM_001162416 NM_008825 NM_001368843 RefSeq (protein) NP_001018063 NP_006203 NP_001155887 NP_001155888 NP_032851 NP_001355772 Location (UCSC) Chr 1: 207.03 – 207.08 Mb Chr 1: 130.62 – 130.66 Mb PubMed search [3] [4]
Wikidata
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6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2 is an enzyme that in humans is encoded by the PFKFB2 gene. ^[5]

The protein encoded by this gene is involved in both the synthesis and degradation of fructose-2,6-bisphosphate, a regulatory molecule that controls glycolysis in eukaryotes. The encoded protein has a 6-phosphofructo-2-kinase activity that catalyzes the synthesis of fructose-2,6-bisphosphate, and a fructose-2,6-biphosphatase activity that catalyzes the degradation of fructose-2,6-bisphosphate. This protein regulates fructose-2,6-bisphosphate levels in the heart, while a related enzyme encoded by a different gene regulates fructose-2,6-bisphosphate levels in the liver and muscle. This enzyme functions as a homodimer. Two transcript variants encoding two different isoforms have been found for this gene. ^[5]

Interactions

PFKFB2 has been shown to interact with YWHAQ. ^[6]

References

1. GRCh38: Ensembl release 89: ENSG00000123836 – Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000026409 – Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. "Entrez Gene: PFKFB2 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2".
6. Pozuelo Rubio M, Peggie Mark, Wong Barry H C, Morrice Nick, MacKintosh Carol (Jul 2003). "14-3-3s regulate fructose-2,6-bisphosphate levels by binding to PKB-phosphorylated cardiac fructose-2,6-bisphosphate kinase/phosphatase". EMBO J. 22 (14). England: 3514–23. doi:10.1093/emboj/cdg363. ISSN   0261-4189. PMC   165633 . PMID   12853467.

Further reading

• Rider MH, Vandamme J, Lebeau E, et al. (1992). "The two forms of bovine heart 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase result from alternative splicing". Biochem. J. 285 (2): 405–11. doi:10.1042/bj2850405. PMC   1132803 . PMID   1322130.
• Hilliker CE, Darville MI, Aly MS, et al. (1991). "Human and rat chromosomal localization of two genes for 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase by analysis of somatic cell hybrids and in situ hybridization". Genomics. 10 (4): 867–73. doi:10.1016/0888-7543(91)90174-D. PMID   1655632.
• Goldmuntz EA, Remmers EF, Zha H, et al. (1994). "Genetic map of seven polymorphic markers comprising a single linkage group on rat chromosome 5". Mamm. Genome. 4 (11): 670–5. doi:10.1007/BF00360905. PMID   7904197. S2CID   1892799.
• Hirata T, Kato M, Okamura N, et al. (1998). "Expression of human placental-type 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase in various cells and cell lines". Biochem. Biophys. Res. Commun. 242 (3): 680–4. doi:10.1006/bbrc.1997.8024. PMID   9464277.
• Heine-Suñer D, Díaz-Guillén MA, Lange AJ, Rodríguez de Córdoba S (1998). "Sequence and structure of the human 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase heart isoform gene (PFKFB2)". Eur. J. Biochem. 254 (1): 103–10. doi: 10.1046/j.1432-1327.1998.2540103.x . PMID   9652401.
• Hirata T, Watanabe M, Miura S, et al. (2001). "Inhibition of tumor cell growth by a specific 6-phosphofructo-2-kinase inhibitor, N-bromoacetylethanolamine phosphate, and its analogues". Biosci. Biotechnol. Biochem. 64 (10): 2047–52. doi: 10.1271/bbb.64.2047 . PMID   11129574. S2CID   38413244.
• Kessler R, Eschrich K (2001). "Splice isoforms of ubiquitous 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase in human brain". Brain Res. Mol. Brain Res. 87 (2): 190–5. doi:10.1016/S0169-328X(01)00014-6. PMID   11245921.
• Soejima H, Kawamoto S, Akai J, et al. (2001). "Isolation of novel heart-specific genes using the BodyMap database". Genomics. 74 (1): 115–20. doi:10.1006/geno.2001.6527. PMID   11374908.
• Baltrusch S, Lenzen S, Okar DA, et al. (2001). "Characterization of glucokinase-binding protein epitopes by a phage-displayed peptide library. Identification of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase as a novel interaction partner". J. Biol. Chem. 276 (47): 43915–23. doi: 10.1074/jbc.M105470200 . PMID   11522786.
• Marsin AS, Bouzin C, Bertrand L, Hue L (2002). "The stimulation of glycolysis by hypoxia in activated monocytes is mediated by AMP-activated protein kinase and inducible 6-phosphofructo-2-kinase". J. Biol. Chem. 277 (34): 30778–83. doi: 10.1074/jbc.M205213200 . hdl: 2078.1/12913 . PMID   12065600.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi: 10.1073/pnas.242603899 . PMC   139241 . PMID   12477932.
• Pozuelo Rubio M, Peggie M, Wong BH, et al. (2003). "14-3-3s regulate fructose-2,6-bisphosphate levels by binding to PKB-phosphorylated cardiac fructose-2,6-bisphosphate kinase/phosphatase". EMBO J. 22 (14): 3514–23. doi:10.1093/emboj/cdg363. PMC   165633 . PMID   12853467.
• Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi: 10.1038/ng1285 . PMID   14702039.
• Jin J, Smith FD, Stark C, et al. (2004). "Proteomic, functional, and domain-based analysis of in vivo 14-3-3 binding proteins involved in cytoskeletal regulation and cellular organization". Curr. Biol. 14 (16): 1436–50. Bibcode:2004CBio...14.1436J. doi: 10.1016/j.cub.2004.07.051 . PMID   15324660. S2CID   2371325.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC   528928 . PMID   15489334.
• Gregory SG, Barlow KF, McLay KE, et al. (2006). "The DNA sequence and biological annotation of human chromosome 1". Nature. 441 (7091): 315–21. Bibcode:2006Natur.441..315G. doi: 10.1038/nature04727 . PMID   16710414.