Paleohistology is the study of the microstructure of fossilized skeletal tissues, offering insights into the biology, growth patterns, and physiology of extinct organisms.
Despite the decay of organic components, the inorganic elements of bone preserve critical structures such as osteocyte lacunae, vascular canals, and collagen fibers. This highly specialized field within paleontology yields insights into the lives of extinct animals, including growth history and age at death. [1] [2] [3] [4]
The microscopic study of biological tissues traces back to 1828 when Henry Witham and William Nicol pioneered techniques for examining petrified tree trunks under a microscope. Subsequently, Louis Agassiz applied these methods to fossil vertebrates. In 1849, John Thomas Quekett published a seminal paper detailing the histological structure of bone across various vertebrate groups, laying the foundation for further research [5] .
Gideon Mantell made significant contributions to paleohistology in the mid-19th century. In 1850, Mantell provided the first clear description of dinosaur bone microstructure, including thin sections from a "dorsal dermal spine" of Hylaerosaurus and a humerus of Pelorosaurus [6] [7] . These observations marked a pivotal moment in the study of ancient tissues, highlighting the preservation of intricate structures in fossilized bone.
Throughout the 20th century, technological advancements revolutionized paleohistology. The introduction of hard plastic resins, tungsten carbide microtome blades, and diamond-edged saw blades enabled researchers to produce thinner sections and conduct more detailed analyses of mineralized tissues. These innovations expanded the scope of paleohistological research, facilitating the examination of fully mineralized bone samples.
In the 1960s and 1970s, Armand de Ricqlès made significant strides in paleohistology by correlating histological features with growth rates and thermal physiology in extinct organisms. Drawing from neontological observations, de Ricqlès demonstrated that avascular bone is deposited more slowly than vascular bone, with implications for understanding the physiology of extinct taxa. His work on dinosaur bone histology suggested physiological similarities between dinosaurs and endothermic birds, challenging prevailing notions of reptilian physiology.
Recent studies in paleohistology have expanded our understanding of ancient tissues, with a focus on quantitative analyses, comparative histology, and interdisciplinary approaches. Ongoing research continues to uncover new insights into the biology and evolution of extinct organisms, leveraging advancements in imaging technology and analytical techniques [8] .
Paleohistologists employ a variety of techniques to study ancient tissues, including thin sectioning, histological staining, and microscopy. Thin sectioning involves cutting slices of fossilized bone or tooth tissue, which are then mounted on slides and examined under a microscope. Histological staining techniques allow researchers to visualize different tissue types, such as bone, cartilage, and teeth, while microscopy enables detailed examination of cellular structures [9] .
Recent advances in imaging technology, such as confocal microscopy and synchrotron radiation, have revolutionized paleohistology by providing higher resolution imaging and non-destructive analysis of fossil specimens [10] .
Paleohistology has diverse applications in paleontology, evolutionary biology, and related fields. By analyzing the microstructure of fossilized tissues, paleohistologists can infer growth rates, metabolic rates, and physiological adaptations of extinct organisms. This information contributes to our understanding of vertebrate evolution, including the origins of flight in birds, the evolution of mammalian reproduction, and the diversity of dinosaurian growth strategies [10] . [2]
Additionally, paleohistological data can provide insights into paleoecological dynamics, such as population demographics, habitat preferences, and responses to environmental change. By reconstructing past environments and ecosystems, paleohistology helps scientists understand the long-term effects of climate change, mass extinctions, and other evolutionary processes [2] .
Tyrannosaurus is a genus of large theropod dinosaur. The type species Tyrannosaurus rex, often shortened to T. rex or colloquially T-Rex, is one of the best represented theropods. It lived throughout what is now western North America, on what was then an island continent known as Laramidia. Tyrannosaurus had a much wider range than other tyrannosaurids. Fossils are found in a variety of rock formations dating to the latest Campanian-Maastrichtian ages of the Late Cretaceous period, 72.7 to 66 million years ago. It was the last known member of the tyrannosaurids and among the last non-avian dinosaurs to exist before the Cretaceous–Paleogene extinction event.
Tyrannosauridae is a family of coelurosaurian theropod dinosaurs that comprises two subfamilies containing up to thirteen genera, including the eponymous Tyrannosaurus. The exact number of genera is controversial, with some experts recognizing as few as three. All of these animals lived near the end of the Cretaceous Period and their fossils have been found only in North America and Asia.
Iguanodon, named in 1825, is a genus of iguanodontian dinosaur. While many species found worldwide have been classified in the genus Iguanodon, dating from the Late Jurassic to Early Cretaceous, taxonomic revision in the early 21st century has defined Iguanodon to be based on one well-substantiated species: I. bernissartensis, which lived during the Barremian to early Aptian ages of the Early Cretaceous in Belgium, Germany, England, and Spain, between about 126 and 122 million years ago. Iguanodon was a large, bulky herbivore, measuring up to 9–11 metres (30–36 ft) in length and 4.5 metric tons in body mass. Distinctive features include large thumb spikes, which were possibly used for defense against predators, combined with long prehensile fifth fingers able to forage for food.
Theropoda (; from ancient Greek θηρίο-ποδός [θηρίον, "wild beast"; πούς, ποδός "foot"] whose members are known as theropods, is a dinosaur clade that is characterized by hollow bones and three toes and claws on each limb. Theropods are generally classed as a group of saurischian dinosaurs. They were ancestrally carnivorous, although a number of theropod groups evolved to become herbivores and omnivores. Theropods first appeared during the Carnian age of the late Triassic period 231.4 million years ago and included the majority of large terrestrial carnivores from the Early Jurassic until at least the close of the Cretaceous, about 66 Ma. In the Jurassic, birds evolved from small specialized coelurosaurian theropods, and are today represented by about 11,000 living species.
Gorgosaurus is a genus of tyrannosaurid theropod dinosaur that lived in western North America during the Late Cretaceous Period (Campanian), between about 76.6 and 75.1 million years ago. Fossil remains have been found in the Canadian province of Alberta and the U.S. state of Montana. Paleontologists recognize only the type species, G. libratus, although other species have been erroneously referred to the genus.
Hylaeosaurus is a herbivorous ankylosaurian dinosaur that lived about 136 million years ago, in the late Valanginian stage of the early Cretaceous period of England. It was found in the Grinstead Clay Formation.
In osteology, the osteon or haversian system is the fundamental functional unit of much compact bone. Osteons are roughly cylindrical structures that are typically between 0.25 mm and 0.35 mm in diameter. Their length is often hard to define, but estimates vary from several millimeters to around 1 centimeter. They are present in many bones of most mammals and some bird, reptile, and amphibian species.
Short bones are designated as those bones that are more or less equal in length, width, and thickness. They include the tarsals in the ankle and the carpals in the wrist. They are one of five types of bones: short, long, flat, irregular and sesamoid. Most short bones are named according to their shape as they exhibit a variety of complex morphological features
Mary Higby Schweitzer is an American paleontologist at North Carolina State University, who led the groups that discovered the remains of blood cells in dinosaur fossils and later discovered soft tissue remains in the Tyrannosaurus rex specimen MOR 1125, as well as evidence that the specimen was a pregnant female when she died.
Tyrannosaurinae is one of the two extinct subfamilies of Tyrannosauridae, a family of coelurosaurian theropods that consists of at least three tribes and several genera. All fossils of these genera have been found in the Late Cretaceous deposits of western North America and east Asia. Compared to the related subfamily Albertosaurinae, tyrannosaurines overall are more robust and larger though the alioramins were gracile by comparison. This subfamily also includes the oldest known tyrannosaurid genus Lythronax as well as the youngest and most famous member of the group, Tyrannosaurus rex. There were at least 30 different species of tyrannosaurines.
Phylogenetic bracketing is a method of inference used in biological sciences. It is used to infer the likelihood of unknown traits in organisms based on their position in a phylogenetic tree. One of the main applications of phylogenetic bracketing is on extinct organisms, known only from fossils, going back to the last universal common ancestor (LUCA). The method is often used for understanding traits that do not fossilize well, such as soft tissue anatomy, physiology and behaviour. By considering the closest and second-closest well-known organisms, traits can be asserted with a fair degree of certainty, though the method is extremely sensitive to problems from convergent evolution.
Tyrannosaurus is one of the most iconic dinosaurs and is known from numerous specimens, some of which have individually acquired notability due to their scientific significance and media coverage.
Kevin Padian is an American paleontologist. He is Professor of Integrative Biology at the University of California, Berkeley, Curator of Paleontology, University of California Museum of Paleontology, and was President of the National Center for Science Education from 2007 to 2008. Padian's area of interest is in vertebrate evolution, especially the origins of flight and the evolution of birds from theropod dinosaurs. He served as an expert witness for the plaintiffs in the Kitzmiller v. Dover Area School District trial, and his testimony was repeatedly cited in the court's decision.
Dinosaur eggs are the organic vessels in which a dinosaur embryo develops. When the first scientifically documented remains of non-avian dinosaurs were being described in England during the 1820s, it was presumed that dinosaurs had laid eggs because they were reptiles. In 1859, the first scientifically documented dinosaur egg fossils were discovered in France by Jean-Jacques Poech, although they were mistaken for giant bird eggs.
Age determination in dinosaurs is mainly used to determine the approximate age of a dinosaur when the animal died.
Armand de Ricqlès is a French paleontologist best known for his work in bone histology and its implications for the growth of dinosaurs (e.g.).
Vivian de Buffrénil is a French histologist and paleobiologist who has worked at the Muséum National d'Histoire Naturelle in Paris from 1982 to 2021. His doctorate (1980) and his doctorat d'état (1990), a diploma now replaced by the habilitation, were supervised by Armand de Ricqlès. His main fields of interest include basic histological descriptions, growth dynamics as recorded in bone growth marks, and adaptation of the tetrapod skeleton to a secondarily aquatic lifestyle. He is also interested in life history and population dynamics of exploited or threatened reptile taxa, especially among Varanidae and Crocodilia. He has published at least 92 papers, including 76 research papers, 10 reports on exploited or threatened species, and six popular papers.
Anusuya Chinsamy-Turan is a South African vertebrate paleontologist known for her expertise and developments in the study of the microstructure of fossil teeth and bones of extinct and extant vertebrates. She was the head of the Department of Biological Sciences, at the University of Cape Town from 2012 to 2015.
Osteoderms are dermal bone structures that support the upper layer of skin and serve as protection against the elements in a large variety of extinct and extant organisms, especially reptiles. This structure is commonly called "dermal armor" and serves to protect the organism, while also helping with temperature regulation. Osteoderms represent hard tissue components of the integument, making them easy to identify in fossil examination. This dermal armor is found prominently in many lizards. Some early amphibians have this armor, but it is lost in modern species with the exception a ventral plate, called the gastralia.
Holly Woodward Ballard is an American paleontologist and paleohistologist.