Rad50

For the computer character encoding, see RADIX-50.

RAD50
Identifiers
Aliases	RAD50 , NBSLD, RAD502, hRad50, Rad50, RAD50 double strand break repair protein
External IDs	OMIM: 604040, 613078; MGI: 109292; HomoloGene: 38092; GeneCards: RAD50; OMA:RAD50 - orthologs
Gene location (Human) Chr. Chromosome 5 (human) [1] Band 5q31.1 Start 132,556,019 bp [1] End 132,646,349 bp [1]
Gene location (Mouse) Chr. Chromosome 11 (mouse) [2] Band 11 B1.3|11 31.98 cM Start 53,540,346 bp [2] End 53,598,146 bp [2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in corpus callosum Achilles tendon epithelium of colon testicle gonad islet of Langerhans stromal cell of endometrium ventricular zone tonsil sural nerve Top expressed in tail of embryo genital tubercle zygote primary oocyte secondary oocyte internal carotid artery Ileal epithelium epiblast Paneth cell ovary More reference expression data BioGPS More reference expression data
Gene ontology Molecular function DNA binding protein-macromolecule adaptor activity nucleotide binding DNA helicase activity metal ion binding single-stranded DNA endodeoxyribonuclease activity ATPase activity protein binding 3'-5' exonuclease activity hydrolase activity ATP binding double-stranded telomeric DNA binding adenylate kinase activity G-quadruplex DNA binding single-stranded telomeric DNA binding Cellular component site of double-strand break membrane nucleoplasm chromosome telomere nucleus condensed nuclear chromosome Mre11 complex Biological process reciprocal meiotic recombination DNA recombination telomere maintenance via telomerase positive regulation of kinase activity telomeric 3' overhang formation cellular response to DNA damage stimulus positive regulation of telomere maintenance negative regulation of telomere capping DNA duplex unwinding meiosis double-strand break repair positive regulation of protein autophosphorylation cell cycle viral process double-strand break repair via nonhomologous end joining telomere maintenance regulation of mitotic recombination nucleic acid phosphodiester bond hydrolysis DNA replication DNA double-strand break processing telomere maintenance via recombination DNA synthesis involved in DNA repair chromosome organization involved in meiotic cell cycle regulation of signal transduction by p53 class mediator nucleotide phosphorylation DNA repair double-strand break repair via homologous recombination strand displacement telomere capping nucleoside monophosphate phosphorylation Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 10111 19360 Ensembl ENSG00000113522 ENSMUSG00000020380 UniProt Q92878 P70388 RefSeq (mRNA) NM_133482 NM_005732 NM_009012 RefSeq (protein) NP_005723 n/a Location (UCSC) Chr 5: 132.56 – 132.65 Mb Chr 11: 53.54 – 53.6 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

DNA repair protein RAD50, also known as RAD50, is a protein that in humans is encoded by the RAD50 gene. ^[5]

Function

The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Rad50, a protein involved in DNA double-strand break repair. This protein forms a complex with MRE11 and NBS1 (also known as Xrs2 in yeast). This MRN complex (MRX complex in yeast) binds to broken DNA ends and displays numerous enzymatic activities that are required for double-strand break repair by nonhomologous end-joining or homologous recombination. Gene knockout studies of the mouse homolog of Rad50 suggest it is essential for cell growth and viability. Two alternatively spliced transcript variants of Rad50, which encode distinct proteins, have been reported. ^[5]

Structure

Rad50 is a member of the structural maintenance of chromosomes (SMC) family of proteins. ^[6] Like other SMC proteins, Rad50 contains a long internal coiled-coil domain that folds back on itself, bringing the N- and C-termini together to form a globular ABC ATPase head domain. Rad50 can dimerize both through its head domain and through a zinc-binding dimerization motif at the opposite end of the coiled-coil known as the “zinc-hook”. ^[7] Results from atomic force microscopy suggest that in free Mre11-Rad50-Nbs1 complexes, the zinc-hooks of a single Rad50 dimer associate to form a closed loop, while the zinc-hooks snap apart upon binding DNA, adopting a conformation that is thought to enable zinc-hook-mediated tethering of broken DNA ends. ^[8]

Interactions

Rad50 has been shown to interact with:

• BRCA1, ^{[9] [10] [11]}
• MRE11A, ^{[9] [10] [12] [13] [14]}
• NBN, ^{[9] [13] [15] [16]}
• RINT1, ^[17]
• TERF2IP, ^[18] and
• TERF2. ^{[18] [19]}

Evolutionary ancestry

Rad50 protein has been mainly studied in eukaryotes. However, recent work has shown that orthologs of the Rad50 protein are also conserved in extant prokaryotic archaea where they likely function in homologous recombinational repair. ^[20] In the hyperthermophilic archeon Sulfolobus acidocaldarius , the Rad50 and Mre11 proteins interact and appear to have an active role in repair of DNA damages introduced by gamma radiation. ^[21] These findings suggest that eukaryotic Rad50 may be descended from an ancestral archaeal Rad50 protein that served a role in homologous recombinational repair of DNA damage. In yeast, the functions controlled by the RAD50 gene are essential for normal meiosis. ^[22] It appears that the normal functions specified by RAD50 are not essential for either the initial or terminal steps in meiosis, but are required for successful recombination. ^[22]

Diseases

Human RAD50 deficiency is an autosomal recessive syndrome that has been reported in patients with microcephaly and short stature. Their clinical phenotype resembled Nijmegen Breakage Syndrome. Cells from these patients showed increased radiosensitity with an impaired response to chromosome breaks. ^{[23] [24] [25]}

See also

• MRN complex
• Homologous recombination
• Nijmegen Breakage Syndrome

References

1. GRCh38: Ensembl release 89: ENSG00000113522 – Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000020380 – Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. "Entrez Gene: RAD50 RAD50 homolog (S. cerevisiae)".
6. Kinoshita E, van der Linden E, Sanchez H, Wyman C (2009). "RAD50, an SMC family member with multiple roles in DNA break repair: how does ATP affect function?". Chromosome Res. 17 (2): 277–88. doi:10.1007/s10577-008-9018-6. PMC   4494100 . PMID   19308707.
7. Hopfner KP, Craig L, Moncalian G, Zinkel RA, Usui T, Owen BA, Karcher A, Henderson B, Bodmer JL, McMurray CT, Carney JP, Petrini JH, Tainer JA (August 2002). "The Rad50 zinc-hook is a structure joining Mre11 complexes in DNA recombination and repair". Nature. 418 (6897): 562–6. Bibcode:2002Natur.418..562H. doi:10.1038/nature00922. PMID   12152085. S2CID   4414704.
8. Moreno-Herrero F, de Jager M, Dekker NH, Kanaar R, Wyman C, Dekker C (September 2005). "Mesoscale conformational changes in the DNA-repair complex Rad50/Mre11/Nbs1 upon binding DNA". Nature. 437 (7057): 440–3. Bibcode:2005Natur.437..440M. doi:10.1038/nature03927. PMID   16163361. S2CID   4357195.
9. Wang Y, Cortez D, Yazdi P, Neff N, Elledge SJ, Qin J (2000). "BASC, a super complex of BRCA1-associated proteins involved in the recognition and repair of aberrant DNA structures". Genes Dev. 14 (8): 927–39. doi:10.1101/gad.14.8.927. PMC   316544 . PMID   10783165.
10. Chiba N, Parvin JD (2001). "Redistribution of BRCA1 among four different protein complexes following replication blockage". J. Biol. Chem. 276 (42): 38549–54. doi: 10.1074/jbc.M105227200 . PMID   11504724.
11. Zhong Q, Chen CF, Li S, Chen Y, Wang CC, Xiao J, Chen PL, Sharp ZD, Lee WH (1999). "Association of BRCA1 with the hRad50-hMre11-p95 complex and the DNA damage response". Science. 285 (5428): 747–50. doi:10.1126/science.285.5428.747. PMID   10426999.
12. Dolganov GM, Maser RS, Novikov A, Tosto L, Chong S, Bressan DA, Petrini JH (1996). "Human Rad50 is physically associated with human Mre11: identification of a conserved multiprotein complex implicated in recombinational DNA repair". Mol. Cell. Biol. 16 (9): 4832–41. doi:10.1128/MCB.16.9.4832. PMC   231485 . PMID   8756642.
13. Trujillo KM, Yuan SS, Lee EY, Sung P (1998). "Nuclease activities in a complex of human recombination and DNA repair factors Rad50, Mre11, and p95". J. Biol. Chem. 273 (34): 21447–50. doi: 10.1074/jbc.273.34.21447 . PMID   9705271.
14. Goedecke W, Eijpe M, Offenberg HH, van Aalderen M, Heyting C (1999). "Mre11 and Ku70 interact in somatic cells, but are differentially expressed in early meiosis". Nat. Genet. 23 (2): 194–8. doi:10.1038/13821. PMID   10508516. S2CID   13443404.
15. Cerosaletti KM, Concannon P (2003). "Nibrin forkhead-associated domain and breast cancer C-terminal domain are both required for nuclear focus formation and phosphorylation". J. Biol. Chem. 278 (24): 21944–51. doi: 10.1074/jbc.M211689200 . PMID   12679336.
16. Desai-Mehta A, Cerosaletti KM, Concannon P (2001). "Distinct functional domains of nibrin mediate Mre11 binding, focus formation, and nuclear localization". Mol. Cell. Biol. 21 (6): 2184–91. doi:10.1128/MCB.21.6.2184-2191.2001. PMC   86852 . PMID   11238951.
17. Xiao J, Liu CC, Chen PL, Lee WH (2001). "RINT-1, a novel Rad50-interacting protein, participates in radiation-induced G(2)/M checkpoint control". J. Biol. Chem. 276 (9): 6105–11. doi: 10.1074/jbc.M008893200 . PMID   11096100.
18. O'Connor MS, Safari A, Liu D, Qin J, Songyang Z (2004). "The human Rap1 protein complex and modulation of telomere length". J. Biol. Chem. 279 (27): 28585–91. doi: 10.1074/jbc.M312913200 . PMID   15100233.
19. Zhu XD, Küster B, Mann M, Petrini JH, de Lange T (2000). "Cell-cycle-regulated association of RAD50/MRE11/NBS1 with TRF2 and human telomeres". Nat. Genet. 25 (3): 347–52. doi:10.1038/77139. PMID   10888888. S2CID   6689794.
20. White MF (January 2011). "Homologous recombination in the archaea: the means justify the ends". Biochem. Soc. Trans. 39 (1): 15–9. doi:10.1042/BST0390015. PMID   21265740. S2CID   239399.
21. Quaiser A, Constantinesco F, White MF, Forterre P, Elie C (2008). "The Mre11 protein interacts with both Rad50 and the HerA bipolar helicase and is recruited to DNA following gamma irradiation in the archaeon Sulfolobus acidocaldarius". BMC Mol. Biol. 9: 25. doi: 10.1186/1471-2199-9-25 . PMC   2288612 . PMID   18294364.
22. Game JC, Zamb TJ, Braun RJ, Resnick M, Roth RM (January 1980). "The Role of Radiation (rad) Genes in Meiotic Recombination in Yeast". Genetics. 94 (1): 51–68. doi:10.1093/genetics/94.1.51. PMC   1214137 . PMID   17248996.
23. Waltes R, Kalb R, Gatei M, Kijas AW, Stumm M, Sobeck A, Wieland B, Varon R, Lerenthal Y, Lavin MF, Schindler D, Dörk T (2009). "Human RAD50 deficiency in a Nijmegen Breakage Syndrome-like disorder". Am. J. Hum. Genet. 84 (5): 605–16. doi:10.1016/j.ajhg.2009.04.010. PMC   2681000 . PMID   19409520.
24. Ragamin A, Yigit G, Bousset K, Beleggia F, Verheijen FW, de Wit MY, Strom TM, Dörk T, Wollnik B, Mancini GM (2020). "Human RAD50 deficiency: Confirmation of a distinctive phenotype". Am. J. Med. Genet. 182 (6): 1378–86. doi:10.1002/ajmg.a.61570. PMC   7318339 . PMID   32212377.
25. Chansel-Da Cruz M, Hohl M, Ceppi I, Kermasson L, Maggiorella L, Modesti M, de Villartay J, Ileri T, Cejka P, Petrini J, Revy P (2020). "A Disease-Causing Single Amino Acid Deletion in the Coiled-Coil Domain of RAD50 Impairs MRE11 Complex Functions in Yeast and Humans". Cell Rep. 33 (13): 108559. doi:10.1016/j.celrep.2020.108559. PMC   7788285 . PMID   33378670.

Further reading

• Stracker TH, Theunissen JW, Morales M, Petrini JH (2005). "The Mre11 complex and the metabolism of chromosome breaks: the importance of communicating and holding things together". DNA Repair (Amst.). 3 (8–9): 845–54. doi:10.1016/j.dnarep.2004.03.014. PMID   15279769.
• Dolganov GM, Maser RS, Novikov A, et al. (1996). "Human Rad50 is physically associated with human Mre11: identification of a conserved multiprotein complex implicated in recombinational DNA repair". Mol. Cell. Biol. 16 (9): 4832–41. doi:10.1128/MCB.16.9.4832. PMC   231485 . PMID   8756642.
• Maser RS, Monsen KJ, Nelms BE, Petrini JH (1997). "hMre11 and hRad50 nuclear foci are induced during the normal cellular response to DNA double-strand breaks". Mol. Cell. Biol. 17 (10): 6087–96. doi:10.1128/MCB.17.10.6087. PMC   232458 . PMID   9315668.
• Carney JP, Maser RS, Olivares H, et al. (1998). "The hMre11/hRad50 protein complex and Nijmegen breakage syndrome: linkage of double-strand break repair to the cellular DNA damage response". Cell. 93 (3): 477–86. doi: 10.1016/S0092-8674(00)81175-7 . PMID   9590181. S2CID   14548642.
• Paull TT, Gellert M (1998). "The 3' to 5' exonuclease activity of Mre 11 facilitates repair of DNA double-strand breaks". Mol. Cell. 1 (7): 969–79. doi: 10.1016/S1097-2765(00)80097-0 . PMID   9651580.
• Trujillo KM, Yuan SS, Lee EY, Sung P (1998). "Nuclease activities in a complex of human recombination and DNA repair factors Rad50, Mre11, and p95". J. Biol. Chem. 273 (34): 21447–50. doi: 10.1074/jbc.273.34.21447 . PMID   9705271.
• Paull TT, Gellert M (1999). "Nbs1 potentiates ATP-driven DNA unwinding and endonuclease cleavage by the Mre11/Rad50 complex". Genes Dev. 13 (10): 1276–88. doi:10.1101/gad.13.10.1276. PMC   316715 . PMID   10346816.
• Kim KK, Shin BA, Seo KH, et al. (1999). "Molecular cloning and characterization of splice variants of human RAD50 gene". Gene. 235 (1–2): 59–67. doi: 10.1016/S0378-1119(99)00215-2 . PMID   10415333.
• Zhong Q, Chen CF, Li S, et al. (1999). "Association of BRCA1 with the hRad50-hMre11-p95 complex and the DNA damage response". Science. 285 (5428): 747–50. doi:10.1126/science.285.5428.747. PMID   10426999.
• Wang Y, Cortez D, Yazdi P, et al. (2000). "BASC, a super complex of BRCA1-associated proteins involved in the recognition and repair of aberrant DNA structures". Genes Dev. 14 (8): 927–39. doi:10.1101/gad.14.8.927. PMC   316544 . PMID   10783165.
• Gatei M, Young D, Cerosaletti KM, et al. (2000). "ATM-dependent phosphorylation of nibrin in response to radiation exposure". Nat. Genet. 25 (1): 115–9. doi:10.1038/75508. PMID   10802669. S2CID   23521589.
• Zhao S, Weng YC, Yuan SS, et al. (2000). "Functional link between ataxia-telangiectasia and Nijmegen breakage syndrome gene products". Nature. 405 (6785): 473–7. Bibcode:2000Natur.405..473Z. doi:10.1038/35013083. PMID   10839544. S2CID   4428170.
• Zhu XD, Küster B, Mann M, et al. (2000). "Cell-cycle-regulated association of RAD50/MRE11/NBS1 with TRF2 and human telomeres". Nat. Genet. 25 (3): 347–52. doi:10.1038/77139. PMID   10888888. S2CID   6689794.
• Paull TT, Rogakou EP, Yamazaki V, et al. (2001). "A critical role for histone H2AX in recruitment of repair factors to nuclear foci after DNA damage". Curr. Biol. 10 (15): 886–95. doi: 10.1016/S0960-9822(00)00610-2 . PMID   10959836. S2CID   16108315.
• Xiao J, Liu CC, Chen PL, Lee WH (2001). "RINT-1, a novel Rad50-interacting protein, participates in radiation-induced G(2)/M checkpoint control". J. Biol. Chem. 276 (9): 6105–11. doi: 10.1074/jbc.M008893200 . PMID   11096100.
• Desai-Mehta A, Cerosaletti KM, Concannon P (2001). "Distinct functional domains of nibrin mediate Mre11 binding, focus formation, and nuclear localization". Mol. Cell. Biol. 21 (6): 2184–91. doi:10.1128/MCB.21.6.2184-2191.2001. PMC   86852 . PMID   11238951.
• Buscemi G, Savio C, Zannini L, et al. (2001). "Chk2 activation dependence on Nbs1 after DNA damage". Mol. Cell. Biol. 21 (15): 5214–22. doi:10.1128/MCB.21.15.5214-5222.2001. PMC   87245 . PMID   11438675.
• Chiba N, Parvin JD (2001). "Redistribution of BRCA1 among four different protein complexes following replication blockage". J. Biol. Chem. 276 (42): 38549–54. doi: 10.1074/jbc.M105227200 . PMID   11504724.
• Grenon M, Gilbert C, Lowndes NF (2001). "Checkpoint activation in response to double-strand breaks requires the Mre11/Rad50/Xrs2 complex". Nat. Cell Biol. 3 (9): 844–7. doi:10.1038/ncb0901-844. PMID   11533665. S2CID   32286986.
• de Jager M, van Noort J, van Gent DC, et al. (2002). "Human Rad50/Mre11 is a flexible complex that can tether DNA ends". Mol. Cell. 8 (5): 1129–35. doi: 10.1016/S1097-2765(01)00381-1 . PMID   11741547.
• M. Beikzadeh, M.P. Latham (2020). "The dynamic nature of the Mre11-Rad50 DNA break repair complex". Progress in Biophysics and Molecular Biology. 163: 14–22. doi: 10.1016/j.pbiomolbio.2020.10.007 . PMC   8065065 . PMID   33121960.

External links

• RAD50 human gene location in the UCSC Genome Browser .
• RAD50 human gene details in the UCSC Genome Browser .