Sulfolobus

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Sulfolobus
RT8-4.jpg
Electron micrograph of Sulfolobus infected with Sulfolobus virus STSV1. Bar = 1 μm.
Scientific classification OOjs UI icon edit-ltr.svg
Domain: Archaea
Kingdom: Proteoarchaeota
Superphylum: TACK group
Phylum: Thermoproteota
Class: Thermoprotei
Order: Sulfolobales
Family: Sulfolobaceae
Genus: Sulfolobus
Brock, Brock, Belly & Weiss 1972
Type species
Sulfolobus acidocaldarius
Brock et al. 1972
Species

Sulfolobus is a genus of microorganism in the family Sulfolobaceae. It belongs to the archaea domain. [2]

Contents

Sulfolobus species grow in volcanic springs with optimal growth occurring at pH 2–3 and temperatures of 75–80 °C, making them acidophiles and thermophiles respectively. Sulfolobus cells are irregularly shaped and flagellar.

Species of Sulfolobus are generally named after the location from which they were first isolated, e.g. Sulfolobus solfataricus was first isolated in the Solfatara volcano. Other species can be found throughout the world in areas of volcanic or geothermal activity, such as geological formations called mud pots, which are also known as solfatare (plural of solfatara).

Sulfolobus as a model to study the molecular mechanisms of DNA replication

When the first Archaeal genome, Methanococcus jannaschii, had been sequenced completely in 1996, it was found that the genes in the genome of Methanococcus jannaschii involved in DNA replication, transcription, and translation were more related to their counterparts in eukaryotes than to those in other prokaryotes. In 2001, the first genome sequence of Sulfolobus, Sulfolobus solfataricus P2, was published. In P2's genome, the genes related to chromosome replication were likewise found to be more related to those in eukaryotes. These genes include DNA polymerase, primase (including two subunits), MCM, CDC6/ORC1, RPA, RPC, and PCNA. In 2004, the origins of DNA replication of Sulfolobus solfataricus and Sulfolobus acidocaldarius were identified. It showed that both species contained two origins in their genome. This was the first time that more than a single origin of DNA replication had been shown to be used in a prokaryotic cell. The mechanism of DNA replication in archaea is evolutionary conserved, and similar to that of eukaryotes. Sulfolobus is now used as a model to study the molecular mechanisms of DNA replication in Archaea. And because the system of DNA replication in Archaea is much simpler than that in Eukaryota, it was suggested that Archaea could be used as a model to study the much more complex DNA replication in Eukaryota.

Role in biotechnology

Sulfolobus proteins are of interest for biotechnology and industrial use due to their thermostable nature. One application is the creation of artificial derivatives from S. acidocaldarius proteins, named affitins . Intracellular proteins are not necessarily stable at low pH however, as Sulfolobus species maintain a significant pH gradient across the outer membrane. Sulfolobales are metabolically dependent on sulfur: heterotrophic or autotrophic, their energy comes from the oxidation of sulfur and/or cellular respiration in which sulfur acts as the final electron acceptor. For example, S. tokodaii is known to oxidize hydrogen sulfide to sulfate intracellularly.

Phylogeny

The currently accepted taxonomy is based on the List of Prokaryotic names with Standing in Nomenclature (LPSN) [3] and National Center for Biotechnology Information (NCBI) [4]

16S rRNA based LTP_06_2022 [5] [6] [7] 53 marker proteins based GTDB 08-RS214 [8] [9] [10]
Sulfolobaceae

Acidianus

Acidianus brierleyi

Metallosphaera

Saccharolobus

Sulfodiicoccus

Sulfuracidifex

Sulfolobus acidocaldarius

Stygiolobus

Sulfurisphaera

Sulfolobaceae

Sulfodiicoccus Sakai & Kurosawa 2017

Sulfurisphaera Kurosawa et al. 1998

Stygiolobus Segerer et al. 1991

Sulfolobus acidocaldarius Brock et al. 1972

Sulfuracidifex Itoh et al. 2020

Saccharolobus Sakai & Kurosawa 2018

Metallosphaera Huber et al. 1989

Acidianus brierleyi

"Ca. Aramenus" Servín-Garcidueñas & Martínez-Romero 2014

Acidianus Segerer et al. 1986

Genome status

The complete genomes have been sequenced for S. acidocaldarius DSM 639 (2,225,959 nucleotides), [11] S. solfataricus P2 (2,992,245 nucleotides), [12] and S. tokodaii str. 7 (2,694,756 nucleotides). [13]

Genome structure

The archaeon Sulfolobus solfataricus has a circular chromosome that consists of 2,992,245 bp. Another sequenced species, S. tokodaii has a circular chromosome as well but is slightly smaller with 2,694,756 bp. Both species lack the genes ftsZ and minD, which has been characteristic of sequenced Crenarchaeota. They also code for citrate synthase and two subunits of 2-oxoacid:ferredoxin oxidoreductase, which plays the same role as alpha-ketoglutarate dehydrogenase in the TCA (tricarboxylic/Krebs/citric acid) cycle. This indicates that Sulfolobus has a TCA cycle system similar to that found in mitochondria of eukaryotes. Other genes in the respiratory chain which partake in the production of ATP were not similar to what is found in eukaryotes. Cytochrome c is one such example that plays an important role in electron transfer to oxygen in eukaryotes. This was also found in A. pernix K1. Since this step is important for an aerobic microorganism like Sulfolobus, it probably uses a different molecule for the same function or has a different pathway.

Cell structure and metabolism

Sulfolobus can grow either lithoautotrophically by oxidizing sulfur, or chemoheterotrophically using sulfur to oxidize simple reduced carbon compounds. Heterotrophic growth has only been observed, however, in the presence of oxygen. The principle metabolic pathways are a glycolytic pathway, a pentose phosphate pathway, and the TCA cycle.

All Archaea have lipids with ether links between the head group and side chains, making the lipids more resistant to heat and acidity than bacterial and eukaryotic ester-linked lipids. The Sulfolobales are known for unusual tetraether lipids. In Sulfolobales, the ether-linked lipids are joined covalently across the "bilayer," making tetraethers. Technically, therefore, the tetraethers form a monolayer, not a bilayer. The tetraethers help Sulfolobus species survive extreme acid as well as high temperature.

Ecology

S. solfataricus has been found in different areas including Yellowstone National Park, Mount St. Helens, Iceland, Italy, and Russia to name a few. Sulfolobus is located almost wherever there is volcanic activity. They thrive in environments where the temperature is about 80 °C with a pH at about 3 and sulfur present. Another species, S. tokodaii , has been located in an acidic spa in Beppu Hot Springs, Kyushu, Japan. Sediments from ~90m below the seafloor on the Peruvian continental margin are dominated by intact archaeal tetraethers, and a significant fraction of the community is sedimentary archaea taxonomically linked to the crenarchaeal Sulfolobales (Sturt, et al., 2004).

DNA damage response

Exposure of Sulfolobus solfataricus or Sulfolobus acidocaldarius to the DNA damaging agents UV-irradiation, bleomycin or mitomycin C induced cellular aggregation. [14] [15] Other physical stressors, such as pH or temperature shift, did not induce aggregation, suggesting that induction of aggregation is caused specifically by DNA damage. [15] Ajon et al. [14] showed that UV-induced cellular aggregation mediates chromosomal marker exchange with high frequency in S. acidocaldarius. Recombination rates exceeded those of uninduced cultures by up to three orders of magnitude. Wood et al. [16] also showed that UV-irradiation increased the frequency of recombination due to genetic exchange in S. acidocaldarius. Frols et al. [15] [17] and Ajon et al. [14] hypothesized that the UV-inducible DNA transfer process and subsequent homologous recombinational repair represents an important mechanism to maintain chromosome integrity in S. acidocaldarius and S. solfataricus. This response may be a primitive form of sexual interaction, similar to the more well-studied bacterial transformation that is also associated with DNA transfer between cells leading to homologous recombinational repair of DNA damage. [18] [19]

The ups operon

The ups operon of Sulfolobus species is highly induced by UV irradiation. The pili encoded by this operon are employed in promoting cellular aggregation, which is necessary for subsequent DNA exchange between cells, resulting in homologous recombination. A study of the Sulfolobales acidocaldariusups operon showed that one of the genes of the operon, saci-1497, encodes an endonuclease III that nicks UV-damaged DNA; and another gene of the operon, saci-1500, encodes a RecQ-like helicase that is able to unwind homologous recombination intermediates such as Holliday junctions. [20] It was proposed that Saci-1497 and Saci-1500 function in an homologous recombination-based DNA repair mechanism that uses transferred DNA as a template. [20] Thus it is thought that the ups system in combination with homologous recombination provide a DNA damage response which rescues Sulfolobales from DNA damaging threats. [20]

Sulfolobus as a viral host

Lysogenic viruses infect Sulfolobus for protection. The viruses cannot survive in the extremely acidic and hot conditions that Sulfolobus lives in, and so the viruses use Sulfolobus as protection against the harsh elements. This relationship allows the virus to replicate inside the archaea without being destroyed by the environment. The Sulfolobus viruses are temperate or permanent lysogens. Permanent lysogens differ from lysogenic bacteriophages in that the host cells are not lysed after the induction of Fuselloviridae production and eventually return to the lysogenic state. They are also unique in the sense that the genes encoding the structural proteins of the virus are constantly transcribed and DNA replication appears to be induced. The viruses infecting archaea like Sulfolobus have to use a strategy to escape prolonged direct exposure to the type of environment their host lives in, which may explain some of their unique properties.

See also

Related Research Articles

<span class="mw-page-title-main">Extremophile</span> Organisms capable of living in extreme environments

An extremophile is an organism that is able to live in extreme environments, i.e., environments with conditions approaching or expanding the limits of what known life can adapt to, such as extreme temperature, radiation, salinity, or pH level.

<span class="mw-page-title-main">Thermophile</span> Organism that thrives at relatively high temperatures

A thermophile is an organism—a type of extremophile—that thrives at relatively high temperatures, between 41 and 122 °C. Many thermophiles are archaea, though some of them are bacteria and fungi. Thermophilic eubacteria are suggested to have been among the earliest bacteria.

<span class="mw-page-title-main">Horizontal gene transfer</span> Type of nonhereditary genetic change

Horizontal gene transfer (HGT) or lateral gene transfer (LGT) is the movement of genetic material between organisms other than by the ("vertical") transmission of DNA from parent to offspring (reproduction). HGT is an important factor in the evolution of many organisms. HGT is influencing scientific understanding of higher order evolution while more significantly shifting perspectives on bacterial evolution.

<span class="mw-page-title-main">Thermoproteota</span> Phylum of archaea

The Thermoproteota are prokaryotes that have been classified as a phylum of the Archaea domain. Initially, the Thermoproteota were thought to be sulfur-dependent extremophiles but recent studies have identified characteristic Thermoproteota environmental rRNA indicating the organisms may be the most abundant archaea in the marine environment. Originally, they were separated from the other archaea based on rRNA sequences; other physiological features, such as lack of histones, have supported this division, although some crenarchaea were found to have histones. Until recently all cultured Thermoproteota had been thermophilic or hyperthermophilic organisms, some of which have the ability to grow at up to 113°C. These organisms stain Gram negative and are morphologically diverse, having rod, cocci, filamentous and oddly-shaped cells.

A mating system is a way in which a group is structured in relation to sexual behaviour. The precise meaning depends upon the context. With respect to animals, the term describes which males and females mate under which circumstances. Recognised systems include monogamy, polygamy, and promiscuity, all of which lead to different mate choice outcomes and thus these systems affect how sexual selection works in the species which practice them. In plants, the term refers to the degree and circumstances of outcrossing. In human sociobiology, the terms have been extended to encompass the formation of relationships such as marriage.

Recombinases are genetic recombination enzymes.

Microbial genetics is a subject area within microbiology and genetic engineering. Microbial genetics studies microorganisms for different purposes. The microorganisms that are observed are bacteria, and archaea. Some fungi and protozoa are also subjects used to study in this field. The studies of microorganisms involve studies of genotype and expression system. Genotypes are the inherited compositions of an organism. Genetic Engineering is a field of work and study within microbial genetics. The usage of recombinant DNA technology is a process of this work. The process involves creating recombinant DNA molecules through manipulating a DNA sequence. That DNA created is then in contact with a host organism. Cloning is also an example of genetic engineering.

<span class="mw-page-title-main">Sulfolobales</span> Order of archaea

Sulfolobales is an order of archaeans in the class Thermoprotei.

<span class="mw-page-title-main">Sulfolobaceae</span> Family of archaea

Sulfolobaceae are a family of the Sulfolobales belonging to the domain Archaea. The family consists of several genera adapted to survive environmental niches with extreme temperature and low pH conditions.

In taxonomy, Thermococcus is a genus of thermophilic Archaea in the family the Thermococcaceae.

<i>Lipothrixviridae</i> Family of viruses

Lipothrixviridae is a family of viruses in the order Ligamenvirales. Thermophilic archaea in the phylum Thermoproteota serve as natural hosts. There are 11 species in this family, assigned to 4 genera.

Fuselloviridae is a family of viruses. Sulfolobus species, specifically shibatae, solfataricus, and islandicus, serve as natural hosts. There are two genera and nine species in the family. The Fuselloviridae are ubiquitous in high-temperature (≥70 °C), acidic hot springs around the world.

<span class="mw-page-title-main">Prokaryote</span> Unicellular organism lacking a membrane-bound nucleus

A prokaryote is a single-cell organism whose cell lacks a nucleus and other membrane-bound organelles. The word prokaryote comes from the Ancient Greek πρό 'before' and κάρυον 'nut, kernel'. In the two-empire system arising from the work of Édouard Chatton, prokaryotes were classified within the empire Prokaryota. But in the three-domain system, based upon molecular analysis, prokaryotes are divided into two domains: Bacteria and Archaea. Organisms with nuclei are placed in a third domain, Eukaryota.

<span class="mw-page-title-main">Archaea</span> Domain of single-celled organisms

Archaea is a domain of single-celled organisms. These microorganisms lack cell nuclei and are therefore prokaryotes. Archaea were initially classified as bacteria, receiving the name archaebacteria, but this term has fallen out of use.

<span class="mw-page-title-main">Branch migration</span>

Branch migration is the process by which base pairs on homologous DNA strands are consecutively exchanged at a Holliday junction, moving the branch point up or down the DNA sequence. Branch migration is the second step of genetic recombination, following the exchange of two single strands of DNA between two homologous chromosomes. The process is random, and the branch point can be displaced in either direction on the strand, influencing the degree of which the genetic material is exchanged. Branch migration can also be seen in DNA repair and replication, when filling in gaps in the sequence. It can also be seen when a foreign piece of DNA invades the strand.

<span class="mw-page-title-main">Sexual reproduction</span> Biological process

Sexual reproduction is a type of reproduction that involves a complex life cycle in which a gamete with a single set of chromosomes combines with another gamete to produce a zygote that develops into an organism composed of cells with two sets of chromosomes (diploid). This is typical in animals, though the number of chromosome sets and how that number changes in sexual reproduction varies, especially among plants, fungi, and other eukaryotes.

<span class="mw-page-title-main">Bicaudaviridae</span> Family of viruses

Bicaudaviridae is a family of hyperthermophilic archaeal viruses. Members of the genus Acidianus serve as natural hosts. There is only one genus, Bicaudavirus, and one species, Acidianus two-tailed virus, in this family. However, Sulfolobus tengchongensis spindle-shaped viruses 1 and 2 are regarded to belong to this family also.

Sulfolobus metallicus is a coccoid shaped thermophilic archaeon. It is a strict chemolithoautotroph gaining energy by oxidation of sulphur and sulphidic ores into sulfuric acid. Its type strain is Kra 23. It has many uses that take advantage of its ability to grow on metal media under acidic and hot environments.

Saccharolobus solfataricus is a species of thermophilic archaeon. It was transferred from the genus Sulfolobus to the new genus Saccharolobus with the description of Saccharolobus caldissimus in 2018.

Sulfolobus acidocaldarius is a thermoacidophilic archaeon that belongs to the phylum Thermoproteota. S. acidocaldarius was the first Sulfolobus species to be described, in 1972 by Thomas D. Brock and collaborators. This species was found to grow optimally between 75 and 80 °C, with pH optimum in the range of 2-3.

References

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