Rhodococcus erythropolis

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Rhodococcus erythropolis
Scientific classification OOjs UI icon edit-ltr.svg
Domain: Bacteria
Phylum: Actinomycetota
Class: Actinomycetia
Order: Mycobacteriales
Family: Nocardiaceae
Genus: Rhodococcus
Species:
R. erythropolis
Binomial name
Rhodococcus erythropolis
(Gray and Thornton 1928) Goodfellow and Alderson 1979 (Approved Lists 1980)
Type strain
ATCC 4277
CIP 104179
DSM 43066
HAMBI 1953
IEGM 7
IFO 15567
JCM 20419
JCM 3201
LMG 5359
N11
NBRC 15567
NCIB 9158
NCIMB 9158
NCTC 13021
NRRL B-16025
VKM Ac-858
Synonyms
  • "Mycobacterium erythropolis" Gray and Thornton 1928
  • Rhodococcus baikonurensisLi et al. 2004 [1]
  • Rhodococcus degradansŠvec et al. 2015
  • Rhodococcus enclensisDastager et al. 2014
  • Rhodococcus jialingiaeWang et al. 2010
  • Rhodococcus qingshengiiXu et al. 2007

Rhodococcus erythropolis is an aerobic Gram-positive bacterium species in the genus Rhodococcus. The name Rhodococcus erythropolis is derived from its morphogenetic cycle from branching to rod and to coccus morphology, which explains the series of morphological changes this bacterium undergoing during growth and development processes. [2] These bacterium are found in red and orange colonies when observed this explains the species name erythropolis which means "red city" in Greek. [3]

Contents

Characteristics and adaptability

This bacterium is aerobic and can be found in soil surfaces where oxygen is abundant and is described as red and orange colored when they are cultured on a medium. [4] [5] This bacterium is non-motile and have an unusual cell envelope composition characterized by a high mycolic acid content, which helps to elevate the cell surface ability of hydrophobicity. This characteristic of the cell surface helps the bacterium to survive between polar and non-polar media. [6]

Diversity

Rhodococcus strains as well as R. erythropolis variants are widely distributed throughout diverse environments ranging from sea level to Alpine soils, deep sea to coastal sediments and Arctic to Antarctic samples. This wide distribution explains their adaptability and resilience in various ecosystems. [7] R. erythropolis has been isolated from the air of the Russian Space Laboratory Mir along with a large number of other microorganisms that steadily accumulated during the lifespan of the station. [1] Rhodococcus bacteria are known to degrade organic compounds contained in the rubber used aboard the space station with specialized enzymes. [8] This can lead to degradation of critical components and necessitates replacement of the parts or preventive measures dealing with microbial contamination.

History

R. erythropolis was named as Mycobacterium erythropolis, and then later called with other names such as Nocardia erythropolis etc., [3] before changing it to Rhodococcus erythropolis based on its growth and morphological characteristics.

Pathogenicity

There were few cases that are infected by R. erythropolis. This bacterium found on various sites of the body such as skin, [9] eye, and bloodstream, [3] and in sputum of a pulmonary illness patient. Though Rhodococcusspp. are found to be infectious to plants and animals, the R. erythropolis is believed to have minimal pathogenic potential; however, sporadic reports have highlighted its clinically significant involvement in certain cases. [10]

Metabolism

Rhodococcus erythropolis exhibits a metabolic diversity and produces enzymes that are capable which capable to degrade (including oxidation, dehydrogenation, epoxidation, hydrolysis, hydroxylation, dehalogenation and desulfurization) a wide range of hydrophobic compounds, and xenobiotics such as polycyclic aromatic hydrocarbons, polychlorinated biphenyls, and dibenzothiophenes. [7] This metabolic versatility is due to the presence and movement of large plasmids, also due to the multiple homologous enzymes in catabolic pathways, to enrich the bacterium versatility. Due to the bacterium ability of resisting to tough chemicals and breaking down difficult-to-remove compounds like lignin, petroleum, and pesticides, R. erythropolis are called as master of metabolisms and remarkable. Hence it explains the widely usage of the R. eythropolis for degrading environmental pollutions. [6]

R. erythropolis is capable of changing the fatty acid composition of its membrane in response to different carbon sources which helps to maintain membrane fluidity and essential biological functions under different environmental conditions. This explains its ability of resilience and adaptability. [7] This micro-organism exhibits Oligotrophic growth and is referred with this term since its CO2 fixation system is not yet discovered. [11]

Applications in biotechnology

In biotechnological applications, R. erythropolis has proven to be a robust biocatalyst, capable of enduring the presence of deleterious substances commonly faced in organic-aqueous biotransformation systems. This bacterium cells able to tolerate and adapt to solvents under stress conditions to protect cell population from solvent effects, since solvent toxicity is a major factor influencing cell behavior and performance in biotransformation systems. R. erythropolis can naturally grow in contaminated environments, due to this mechanism this bacterium is used in the process of bioremediation. [7] Apart from the bacterium role in bioremediation, R. erythropolis is capable of acting as biological control in plants. The bacterium's abilities of catabolic mechanisms and degrading abilities are used to disrupt quorum sensing mediated communication of gram negative pathogens that affects plants particularly in phyllosphere and rhizosphere. The role of R. erythropolis as a biological control in plants explains its application in agriculture and environmental applications. [6]

Genomics

R. erythropolis bacterium genome consists of one main chromosome with 6,455,263 bp, one linear plasmid(227,989bp), and three circular plasmids with ([79,600bp], [5,420bp], [5,444bp]). The G+C contents occupies 59 to 62% genome. The whole genome contains 6, 318 putative coding sequences, 60 tRNAs, and 5 rrn operons. [12]

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References

  1. 1 2 Li Y, Kawamura Y, Fujiwara N, Naka T, Liu H, Huang X, et al. (May 2004). "Rothia aeria sp. nov., Rhodococcus baikonurensis sp. nov. and Arthrobacter russicus sp. nov., isolated from air in the Russian space laboratory Mir". International Journal of Systematic and Evolutionary Microbiology. 54 (Pt 3): 827–835. doi: 10.1099/ijs.0.02828-0 . PMID   15143031.
  2. ↑ Goodfellow M, Alderson G, Chun J (1998). "Rhodococcal systematics: problems and developments". Antonie van Leeuwenhoek. 74 (1–3): 3–20. doi:10.1023/a:1001730725003. PMID   10068784.
  3. 1 2 3 Baba H, Nada T, Ohkusu K, Ezaki T, Hasegawa Y, Paterson DL (August 2009). "First case of bloodstream infection caused by Rhodococcus erythropolis". Journal of Clinical Microbiology. 47 (8): 2667–2669. doi:10.1128/JCM.00294-09. PMC   2725670 . PMID   19494072.
  4. ↑ Graham JE, Moore JE, Jiru X, Moore JE, Goodall EA, Dooley JS, et al. (December 2007). "Ocular pathogen or commensal: a PCR-based study of surface bacterial flora in normal and dry eyes". Investigative Ophthalmology & Visual Science. 48 (12): 5616–5623. doi:10.1167/iovs.07-0588. PMID   18055811.
  5. ↑ Bell KS, Philp JC, Aw DW, Christofi N (August 1998). "The genus Rhodococcus". Journal of Applied Microbiology. 85 (2): 195–210. doi:10.1046/j.1365-2672.1998.00525.x. PMID   9750292.
  6. 1 2 3 Latour X, Barbey C, Chane A, Groboillot A, Burini JF (December 2013). "Rhodococcus erythropolis and its γ-Lactone Catabolic Pathway: An Unusual Biocontrol System that Disrupts Pathogen Quorum Sensing Communication". Agronomy. 3 (4): 816–838. doi: 10.3390/agronomy3040816 .
  7. 1 2 3 4 de Carvalho CC, da Fonseca MM (June 2005). "The remarkable Rhodococcus erythropolis". Applied Microbiology and Biotechnology. 67 (6): 715–726. doi:10.1007/s00253-005-1932-3. PMID   15711940.
  8. ↑ Bell TE. (2007). "Preventing "Sick" Spaceships". NASA Science. NASA . Retrieved June 25, 2022.
  9. ↑ Vernazza PL, Bodmer T, Galeazzi RL (July 1991). "[Rhodococcus erythropolis infection in HIV-associated immunodeficiency]". Schweizerische Medizinische Wochenschrift. 121 (30): 1095–1098. PMID   1866607.
  10. ↑ Park SD, Uh Y, Jang IH, Yoon KJ, Kim HM, Bae YJ (February 2011). "Rhodococcus erythropolis septicaemia in a patient with acute lymphocytic leukaemia". Journal of Medical Microbiology. 60 (Pt 2): 252–255. doi:10.1099/jmm.0.015412-0. PMID   20965915.
  11. ↑ Ohhata N, Yoshida N, Egami H, Katsuragi T, Tani Y, Takagi H (October 2007). "An extremely oligotrophic bacterium, Rhodococcus erythropolis N9T-4, isolated from crude oil". Journal of Bacteriology. 189 (19): 6824–6831. doi:10.1128/JB.00872-07. PMC   2045210 . PMID   17675378.
  12. ↑ Kitagawa W, Hata M (December 2022). "Complete Genome Sequence of Rhodococcus erythropolis JCM 2895, an Antibiotic Protein-Producing Strain". Microbiology Resource Announcements. 11 (12): e0068222. doi:10.1128/mra.00682-22. PMC   9753674 . PMID   36321907.

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