Histology, also known as microscopic anatomy or microanatomy, is the branch of biology that studies the microscopic anatomy of biological tissues. Histology is the microscopic counterpart to gross anatomy, which looks at larger structures visible without a microscope. Although one may divide microscopic anatomy into organology, the study of organs, histology, the study of tissues, and cytology, the study of cells, modern usage places all of these topics under the field of histology. In medicine, histopathology is the branch of histology that includes the microscopic identification and study of diseased tissue. In the field of paleontology, the term paleohistology refers to the histology of fossil organisms.
A microscope is a laboratory instrument used to examine objects that are too small to be seen by the naked eye. Microscopy is the science of investigating small objects and structures using a microscope. Microscopic means being invisible to the eye unless aided by a microscope.
In archaeology, ground stone is a category of stone tool formed by the grinding of a coarse-grained tool stone, either purposely or incidentally. Ground stone tools are usually made of basalt, rhyolite, granite, or other cryptocrystalline and igneous stones whose coarse structure makes them ideal for grinding other materials, including plants and other stones.
In dentistry, calculus or tartar is a form of hardened dental plaque. It is caused by precipitation of minerals from saliva and gingival crevicular fluid (GCF) in plaque on the teeth. This process of precipitation kills the bacterial cells within dental plaque, but the rough and hardened surface that is formed provides an ideal surface for further plaque formation. This leads to calculus buildup, which compromises the health of the gingiva (gums). Calculus can form both along the gumline, where it is referred to as supragingival, and within the narrow sulcus that exists between the teeth and the gingiva, where it is referred to as subgingival.
The microscopic scale is the scale of objects and events smaller than those that can easily be seen by the naked eye, requiring a lens or microscope to see them clearly. In physics, the microscopic scale is sometimes regarded as the scale between the macroscopic scale and the quantum scale. Microscopic units and measurements are used to classify and describe very small objects. One common microscopic length scale unit is the micrometre, which is one millionth of a metre.
Paleoethnobotany, or archaeobotany, is the study of past human-plant interactions through the recovery and analysis of ancient plant remains. Both terms are synonymous, though paleoethnobotany is generally used in North America and acknowledges the contribution that ethnographic studies have made towards our current understanding of ancient plant exploitation practices, while the term archaeobotany is preferred in Europe and emphasizes the discipline's role within archaeology.
Palynology is the study of microorganisms and microscopic fragments of mega-organisms that are composed of acid-resistant organic material and occur in sediments, sedimentary rocks, and even some metasedimentary rocks. Palynomorphs are the microscopic, acid-resistant organic remains and debris produced by a wide variety plants, animals, and Protista that have existed since the late Proterozoic.
Staining is a technique used to enhance contrast in samples, generally at the microscopic level. Stains and dyes are frequently used in histology, in cytology, and in the medical fields of histopathology, hematology, and cytopathology that focus on the study and diagnoses of diseases at the microscopic level. Stains may be used to define biological tissues, cell populations, or organelles within individual cells.
In archaeology, a blade is a type of stone tool created by striking a long narrow flake from a stone core. This process of reducing the stone and producing the blades is called lithic reduction. Archaeologists use this process of flintknapping to analyze blades and observe their technological uses for historical purposes.
Petrography is a branch of petrology that focuses on detailed descriptions of rocks. Someone who studies petrography is called a petrographer. The mineral content and the textural relationships within the rock are described in detail. The classification of rocks is based on the information acquired during the petrographic analysis. Petrographic descriptions start with the field notes at the outcrop and include macroscopic description of hand-sized specimens. The most important petrographer's tool is the petrographic microscope. The detailed analysis of minerals by optical mineralogy in thin section and the micro-texture and structure are critical to understanding the origin of the rock.
A fluorescence microscope is an optical microscope that uses fluorescence instead of, or in addition to, scattering, reflection, and attenuation or absorption, to study the properties of organic or inorganic substances. "Fluorescence microscope" refers to any microscope that uses fluorescence to generate an image, whether it is a simple set up like an epifluorescence microscope or a more complicated design such as a confocal microscope, which uses optical sectioning to get better resolution of the fluorescence image.
Phytoliths are rigid, microscopic structures made of silica, found in some plant tissues and persisting after the decay of the plant. These plants take up silica from the soil, whereupon it is deposited within different intracellular and extracellular structures of the plant. Phytoliths come in varying shapes and sizes. Although some use "phytolith" to refer to all mineral secretions by plants, it more commonly refers to siliceous plant remains. In contrast, mineralized calcium secretions in cacti are composed of calcium oxalates.
Metallography is the study of the physical structure and components of metals, by using microscopy.
Gunshot residue (GSR), also known as cartridge discharge residue (CDR), gunfire residue (GFR), or firearm discharge residue (FDR), consists of all of the particles that are expelled from the muzzle of a gun following the discharge of a bullet. It is principally composed of burnt and unburnt particles from the explosive primer, the propellant (gunpowder), and vaporized lead. The act of firing a bullet incites a very violent explosive reaction that is contained within the barrel of the gun, which can cause the bullet, the barrel, or the cartridge to become chipped. Meaning gunshot residue may also included metal fragments from the cartridge casing, the bullets jacket, as well as any other dirt or residue contained within the barrel that could have become dislodged.
Post-excavation analysis constitutes processes that are used to study archaeological materials after an excavation is completed. Since the advent of "New Archaeology" in the 1960s, the use of scientific techniques in archaeology has grown in importance. This trend is directly reflected in the increasing application of the scientific method to post-excavation analysis. The first step in post-excavation analysis should be to determine what one is trying to find out and what techniques can be used to provide answers. Techniques chosen will ultimately depend on what type of artifact(s) one wishes to study. This article outlines processes for analyzing different artifact classes and describes popular techniques used to analyze each class of artifact. Keep in mind that archaeologists frequently alter or add techniques in the process of analysis as observations can alter original research questions.
A loupe is a simple, small magnification device used to see small details more closely. They generally have higher magnification than a magnifying glass, and are designed to be held or worn close to the eye. A loupe does not have an attached handle, and its focusing lens(es) are contained in an opaque cylinder or cone. On some loupes this cylinder folds into an enclosing housing that protects the lenses when not in use.
The optical properties of all liquid and solid materials change as a function of the wavelength of light used to measure them. This change as a function of wavelength is called the dispersion of the optical properties. The graph created by plotting the optical property of interest by the wavelength at which it is measured is called a dispersion curve.
Immunogold labeling or Immunogold staining (IGS) is a staining technique used in electron microscopy. This staining technique is an equivalent of the indirect immunofluorescence technique for visible light. Colloidal gold particles are most often attached to secondary antibodies which are in turn attached to primary antibodies designed to bind a specific antigen or other cell component. Gold is used for its high electron density which increases electron scatter to give high contrast 'dark spots'.
Ngalue Cave is an archaeological site located in the Niassa province of Mozambique. Excavated primarily by Julio Mercader in 2007, Ngalue is a Middle Stone Age site. Due to its relatively dry environment and the shape of the cave, Ngalue had very good preservation and not only were stone tools and animal bones found. There were preserved starch grains on many of the stone tools as well. Overall, this site can help add to our knowledge of the Middle Stone Age site in the Niassa valley and to our understanding of the subsistence of Middle Stone Age peoples in Eastern Africa as a whole.
The analysis of dental remains is a valuable tool to archaeologists. Teeth are hard, highly mineralised and chemically stable, so therefore preserve well and are one of the most commonly found animals remains. Analysis of these remains also yields a wealth of information. It can not only be used to determine the sex and age of the individual whose mandibular or dental remains have been found, but can also shed light on their diet, pathology, and even their geographic origins through isotope analysis.
