Sugarcane grassy shoot disease

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Sugarcane grassy shoot disease
Amit Yadav SCGS 040620093043.jpg
Typical symptoms of Sugarcane Grassy Shoot (SCGS) disease
Common namesSCGS, Sugarcane grassy shoot disease; GSD, Grassy Shoot disease
Causal agents'Candidatus Phytoplasma sacchari'
Hosts Sugarcane (Saccharum officinarum L.)
Vectors leafhoppers ( Saccharosydne saccharivora , Matsumuratettix hiroglyphicus , Deltocephalus vulgaris and Yamatotettix flavovittatus )
EPPO Code PHYP48
DistributionSoutheast Asia and India

Sugarcane grassy shoot disease (SCGS), is associated with 'Candidatus Phytoplasma sacchari' which are small, pleomorphic, pathogenic mycoplasma that contribute to yield losses from 5% up to 20% in sugarcane. These losses are higher in the ratoon crop. A higher incidence of SCGS has been recorded in some parts of Southeast Asia and India, resulting in 100% loss in cane yield and sugar production. [1] [2] [3]

Contents

SCGS disease symptoms

Complete leaf chlorosis in SCGS disease Amit Yadav SCGS 00234.JPG
Complete leaf chlorosis in SCGS disease

Phytoplasma-infected sugarcane plants show a proliferation of tillers, which give it typical grassy appearance, hence the name grassy shoot disease. The leaves of infected plants do not produce chlorophyll, and therefore appear white or creamy yellow. The leaf veins turn white first as the phytoplasma resides in leaf phloem tissue. Symptoms at the early stage of the plant life cycle include leaf chlorosis, mainly at the central leaf whorl. Infected plants do not have the capacity to produce food in the absence of chlorophyll, which results in no cane formation. These symptoms can be seen prominently in the stubble crop. The eye or lateral buds sprout before the normal time on growing cane. A survey of various fields of western Maharashtra showed grassy shoot with chlorotic or creamy white leaves was the most prevalent phenotype in sugarcane plants infected with SCGS.

Causal organism

SCGS disease is related to 'Candidatus Phytoplasma sacchari, [4] [5] which is one of the most destructive pathogens of sugarcane (Saccharum officinarum L.). In India, SCGS phytoplasmas are spreading at an alarming rate, adversely affecting the yield of the sugarcane crop. [1] [2] [3] Phytoplasmas formerly called mycoplasma-like organisms (MLOs), are a large group of obligate, intracellular, cell wallless parasites classified within the class Mollicutes. [2] [3] Phytoplasmas are associated with plant diseases and are known to cause more than 600 diseases in several hundred plant species, including gramineous weeds and cereals. [6] [7] [8] The symptoms shown by infected plants include: whitening or yellowing of the leaves, shortening of the internodes (leading to stunted growth), smaller leaves and excessive proliferation of shoots, resulting in a broom phenotype and loss of apical dominance . [6]

Transmission

Sugarcane is a vegetatively propagated crop, so the pathogen is transmitted via seed material and by phloem-feeding leafhopper vectors. [9] [10] Saccharosydne saccharivora , [11] Matsumuratettix hiroglyphicus , [12] Deltocephalus vulgaris [13] and Yamatotettix flavovittatus [14] have been confirmed as vectors for phytoplasma transmission in sugarcane. Unconfirmed reports also suggest a spread through the steel blades (machetes) used for sugarcane harvesting.[ dubious ]

Detection

Phytoplasma-infected sugarcane can be recognized by visual symptoms, but there are limitations. [1] Visual symptoms occur only after considerable growth, normally two to three weeks after planting. [1] If not observed keenly, confusion may occur on differences between symptoms of SCGS disease and iron deficiency. In addition to above points, the poor relationship between symptoms and phytoplasma presence has been confirmed by earlier findings that symptoms alone are not reliable indicators of infection or identity. This highlights the importance of employing tests, such as molecular tests, to verify associations between phytoplasma and putative disease symptoms. Also, it suggests the inability to recognize symptomless sugarcane harbouring a phytoplasma could result in inadvertent exposure of sugarcane to a potential disease source. [15] Precise diagnosis is, therefore, necessary for effective disease identification and control. Though reliable, DNA hybridization, [16] electron microscopy [10] and PCR [17] techniques require specialized equipment and trained human resources. Among these, polymerase chain reaction (PCR) is an accurate, economical and convenient method, which allows analysis of samples in a short time.

In recent years, regions of the rRNA operon of the prokaryotic and eukaryotic organisms have been sequenced and are being used to develop PCR-based detection assays. These sequences are highly specific to the infecting organism.

The ribosomal DNA contains one transcriptional unit with a cluster of genes coding for the 18S, 5.8S and 28S rRNAs and two internal transcribed spacer regions, ITS1 and ITS2 [18] in eukaryotes, and for 16S, 5S and 23S in prokaryotes . Previous studies have demonstrated the complex ITS regions are useful in measuring close genealogical relationships because they exhibit greater interspecies differences than the smaller and larger subunits of rRNA genes. [8] The use of specific probes as selective PCR primers offers an impressive approach for the rapid identification of a large number of phytoplasma isolates. [1] [8] [17] [19] [20] [21]

Sugarcane crop showing iron deficiency Amit Yadav SID P 033.jpg
Sugarcane crop showing iron deficiency
Interveinal chlorosis due to iron deficiency Amit 000 P 010 - Copy.jpg
Interveinal chlorosis due to iron deficiency

Control

In SCGS disease, the primary concern is to prevent the disease rather than treat it. Large numbers of phytoplasma-infected seed sets used by the farmers usually cause fast SCGS disease spread. Healthy, certified 'disease-free' sugarcane sets are suggested as planting material. If disease symptoms are visible within two weeks after planting, such plants can be replaced by healthy plants. Uprooted infected sugarcane plants need to disposed of by burning them.

Moist hot air treatment of sets is suggested to control infection [22] before planting. This reduces the percentage of disease incidence, but causes a reduction in the percentage of bud sprouting. Reports that the disease spreads through steel blades used for sugarcane harvesting are unconfirmed, but treating the knives using a disinfectant or by dipping them in boiling water for some time is suggested as a precaution.

Phytoplasma infection also spreads through insect vectors; it is, therefore, important to control them. General field observation reports the ratoon crop has a higher percentage of disease incidence than the initial planted (main) crop. When the disease incidence is more than 20%, it is suggested to discontinue that crop cycle. It is always wise to purchase the certified planting material from authorized seed growers, which assures disease-free planting material.

Sugarcane iron deficiency and SCGS disease

Symptoms of iron deficiency (interveinal chlorosis) are very similar to those of SCGS. It shows creamy leaves, but no chlorosis occurs in leaf veins, and they remain green. In the case of severe iron deficiency, veins may lose chlorophyll in the absence of iron and appear similar to SCGS disease. [23] [24] Iron deficiency is caused by a lack of iron nutrients in the soil; therefore, one may observe several plants showing symptoms of iron deficiency in localized patches in a field. Phytoplasma-infected plants, though, may occur anywhere in the field in a more random distribution. Treatment with 0.1% ferrous sulfate, either by spraying or supplying it through fertilizer cures iron deficiency, but phytoplasma-infected sugarcane does not respond to any treatment. Phytoplasma-infected plants growing in vitro show sensitivity to tetracycline. [23] [24]

See also

Related Research Articles

<i>Phytoplasma</i> Genus of bacteria

Phytoplasmas are obligate intracellular parasites of plant phloem tissue and of the insect vectors that are involved in their plant-to-plant transmission. Phytoplasmas were discovered in 1967 by Japanese scientists who termed them mycoplasma-like organisms. Since their discovery, phytoplasmas have resisted all attempts at in vitro culture in any cell-free medium; routine cultivation in an artificial medium thus remains a major challenge. Phytoplasmas are characterized by the lack of a cell wall, a pleiomorphic or filamentous shape, a diameter normally less than 1 μm, and a very small genome.

<span class="mw-page-title-main">Acholeplasmataceae</span> Family of bacteria

Acholeplasmataceae is a family of bacteria. It is the only family in the order Acholeplasmatales, placed in the class Mollicutes. The family comprises the genera Acholeplasma and Phytoplasma. Phytoplasma has the candidatus status, because members still could not be cultured.

<i>Xylella fastidiosa</i> Bacteria harming plants, including crops

Xylella fastidiosa is an aerobic, Gram-negative bacterium of the genus Xylella. It is a plant pathogen, that grows in the water transport tissues of plants' and is transmitted exclusively by xylem sap-feeding insects such as sharpshooters and spittlebugs. Many plant diseases are due to infections of X. fastidiosa, including bacterial leaf scorch, oleander leaf scorch, coffee leaf scorch (CLS), alfalfa dwarf, phony peach disease, and the economically important Pierce's disease of grapes (PD), olive quick decline syndrome (OQDS), and citrus variegated chlorosis (CVC). While the largest outbreaks of X. fastidiosa–related diseases have occurred in the Americas and Europe, this pathogen has also been found in Taiwan, Israel, and a few other countries worldwide.

<span class="mw-page-title-main">Aster yellows</span> Plant disease

Aster yellows is a chronic, systemic plant disease caused by several bacteria called phytoplasma. The aster yellows phytoplasma (AYP) affects 300 species in 38 families of broad-leaf herbaceous plants, primarily in the aster family, as well as important cereal crops such as wheat and barley. Symptoms are variable and can include phyllody, virescence, chlorosis, stunting, and sterility of flowers. The aster leafhopper vector, Macrosteles quadrilineatus, moves the aster yellows phytoplasma from plant to plant. Its economic burden is primarily felt in the carrot crop industry, as well as the nursery industry. No cure is known for plants infected with aster yellows. Infected plants should be removed immediately to limit the continued spread of the phytoplasma to other susceptible plants. However, in agricultural settings such as carrot fields, some application of chemical insecticides has proven to minimize the rate of infection by killing the vector.

Xylophilus ampelinus is a species of bacteria that can cause plant disease. It is available from the NCPPB in the United Kingdom and other international culture collections such as ICMP in New Zealand, and LMG/BCCM in Belgium.

<i>Tobacco streak virus</i> Species of virus

Tobacco streak virus (TSV) is a plant pathogenic virus of the family Bromoviridae, in the genus Ilarvirus. It has a wide host range, with at least 200 susceptible species. TSV is generally more problematic in the tropics or warmer climates. TSV does not generally lead to epidemics, with the exception of sunflowers in India and Australia, and peanuts in India.

<span class="mw-page-title-main">Flavescence dorée</span> Disease of plants

Flavescence dorée is one of the most important and damaging phytoplasma diseases of the vine with the potential to threaten vineyards. The bacterial agent has recently been named Candidatus Phytoplasma vitis, and its vector is the leafhopper, Scaphoideus titanus. Infection may kill young vines and greatly reduce the productivity of old vines. It is classified as a phytoplasma disease belonging to the group generically termed grapevine yellows. Occurrences are in sporadic epidemics, and varieties vary in their sensitivity to it.

<span class="mw-page-title-main">Zebra chip</span> Plant disease in potatoes

Zebra chip, also known as papa manchada and papa rayada, is a disease in potatoes putatively caused by an alphaproteobacterium "Candidatus Liberibacter solanacearum", which is vectored by the potato psyllid. When fried, potato tubers from infected plants develop unsightly black lines resembling the stripes of zebras that render the chips unsellable. Additionally, striped sections of chips frequently burn and caramelize, resulting in a bitter flavor. No health risks have been connected with consumption of infected potato chips.

<span class="mw-page-title-main">Grapevine yellows</span> Diseases associated to phytoplasmas

Grapevine yellows (GY) are diseases associated to phytoplasmas that occur in many grape growing areas worldwide and are of still increasing significance. The most important grapevine yellows is flavescence dorée.

<i>Liberibacter</i> Species of bacterium

Liberibacter is a genus of Gram-negative bacteria in the Rhizobiaceae family. Detection of the liberibacteria is based on PCR amplification of their 16S rRNA gene with specific primers. Members of the genus are plant pathogens mostly transmitted by psyllids. The genus was originally spelled Liberobacter.

<i>Mycoplasma haemofelis</i> Parasitic bacterium

Mycoplasma haemofelis is a gram-negative epierythrocytic parasitic bacterium. It often appears in bloodsmears as small (0.6μm) coccoid bodies, sometimes forming short chains of three to eight organisms. It is usually the causative agent of feline infectious anemia (FIA) in the United States.

Deltocephalus is a leafhopper genus in the subfamily Deltocephalinae.

<span class="mw-page-title-main">Cherry X Disease</span>

Cherry X disease also known as Cherry Buckskin disease is caused by a plant pathogenic phytoplasma. Phytoplasmas are obligate parasites of plants and insects. They are specialized bacteria, characterized by their lack of a cell wall, often transmitted through insects, and are responsible for large losses in crops, fruit trees, and ornamentals. The phytoplasma causing Cherry X disease has a fairly limited host range mostly of stone fruit trees. Hosts of the pathogen include sweet cherry, sour cherry, choke cherry, peaches, nectarines, almonds, clover, and dandelion. Most commonly the pathogen is introduced into economical fruit orchards from wild choke cherry and herbaceous weed hosts. The pathogen is vectored by mountain and cherry leafhoppers. The mountain leafhopper vectors the pathogen from wild hosts to cherry orchards but does not feed on the other hosts. The cherry leafhopper feeds on cherry trees and can transmit the disease from cherry orchards to peach, nectarine, and other economic crops. The Saddled Leafhopper is a vector of the disease in peaches. Control of Cherry X disease is limited to controlling the spread, vectors, and weed hosts of the pathogen. Once the pathogen has infected a tree it is fatal and removal is necessary to stop it from becoming a reservoir for vectors.

<i>Candidatus</i> Phytoplasma fraxini Species of bacterium

CandidatusPhytoplasma fraxini is a species of phytoplasma, a specialized group of bacteria which lack a cell wall and attack the phloem of plants. This phytoplasma causes the diseases ash yellows and lilac witches' broom.

Little leaf of brinjal or eggplant is one of the most serious diseases of brinjal in the areas of its cultivation.

Papaya Bunchy Top Disease was first discovered in 1931 in Puerto Rico. Early on, the identity of the pathogen was highly contested due to the inability of isolating it; thus Koch’s postulates could not be fulfilled. Scientists have previously believed that Papaya Bunchy Top Disease was caused by a virus, a mycoplasma-like organism (MLO), or a phytoplasma, but these possible pathogens have since been disproven. Since the identity of the pathogen was unknown, all diagnoses were given solely based on a list of commonly associated symptoms. Through sequencing and microscopy, scientists identified the pathogen to be a part of the genus Rickettsia in 1996. The bacterium is described as being rod-shaped, small, gram-negative, and laticifer-inhibiting. Rickettsia causes diseases in animals, such as typhus and spotted fever, as well as in other plants, such as phony disease of peach and almond leaf scorch. Papaya Bunchy Top is found throughout the American tropics and has been economically important due to its major impact on fruit production. There is little information about the current economic impact.

CandidatusPhytoplasma sacchari is a species of phytoplasma pathogen associated with sugarcane grassy shoot disease (SCGS). This SCGS phytoplasma belongs to the Rice Yellow Dwarf (RYD) group.

Milkweed yellows phytoplasma is a strain of phytoplasma in the class Mollicutes, a class of bacteria distinguished by the absence of a cell wall. The phytoplasma strain is denoted by the acronym MW1.

Candidatus Phytoplasma pruni is a species of phytoplasma in the class Mollicutes, a class of bacteria distinguished by the absence of a cell wall. The specific epithet pruni means "living on Prunus", emphasizing the fact that the phytoplasma is a parasite of various Prunus species, otherwise known as stone fruits. The phytoplasma is commonly called the X-disease phytoplasma.

References

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Further reading