VPS18

VPS18
Identifiers
Aliases	VPS18 , PEP3, CORVET/HOPS core subunit, VPS18 core subunit of CORVET and HOPS complexes
External IDs	OMIM: 608551 MGI: 2443626 HomoloGene: 13302 GeneCards: VPS18
Gene location (Human) Chr. Chromosome 15 (human) [1] Band 15q15.1 Start 40,894,450 bp [1] End 40,903,975 bp [1]
Gene location (Mouse) Chr. Chromosome 2 (mouse) [2] Band 2|2 E5 Start 119,119,221 bp [2] End 119,128,934 bp [2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in pancreatic ductal cell stromal cell of endometrium tibialis anterior muscle blood islet of Langerhans prefrontal cortex gastrocnemius muscle monocyte left ventricle Right adrenal gland Top expressed in Ankle joint yolk sac superior frontal gyrus cerebellar cortex supraoptic nucleus proximal tubule lip neural tube ileum olfactory tubercle More reference expression data BioGPS More reference expression data
Gene ontology Molecular function protein-macromolecule adaptor activity metal ion binding protein binding actin binding syntaxin binding Cellular component HOPS complex AP-3 adaptor complex endosome late endosome membrane late endosome membrane autophagosome lysosomal membrane early endosome actin filament CORVET complex lysosome cytoplasmic vesicle clathrin-coated vesicle presynapse glutamatergic synapse Biological process endosome to lysosome transport endosome organization autophagy protein transport viral entry into host cell intracellular protein transport lysosome organization vesicle-mediated transport vesicle docking involved in exocytosis regulation of SNARE complex assembly transport regulation of synaptic vesicle exocytosis vacuole organization Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 57617 228545 Ensembl ENSG00000104142 ENSMUSG00000034216 UniProt Q9P253 Q8R307 RefSeq (mRNA) NM_020857 NM_172269 RefSeq (protein) NP_065908 NP_065908.1 NP_758473 Location (UCSC) Chr 15: 40.89 – 40.9 Mb Chr 2: 119.12 – 119.13 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

Vacuolar protein sorting-associated protein 18 homolog is a protein that in humans is encoded by the VPS18 gene. ^{[5] [6] [7]}

Function

Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene encodes the human homolog of yeast class C Vps18 protein. The mammalian class C Vps proteins are predominantly associated with late endosomes/lysosomes, and like their yeast counterparts, may mediate vesicle trafficking steps in the endosome/lysosome pathway. ^[7]

Interactions

VPS18 has been shown to interact with VPS11 ^[8] and STX7. ^[8]

References

1. GRCh38: Ensembl release 89: ENSG00000104142 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000034216 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Huizing M, Didier A, Walenta J, Anikster Y, Gahl WA, Krämer H (March 2001). "Molecular cloning and characterization of human VPS18, VPS 11, VPS16, and VPS33". Gene. 264 (2): 241–7. doi:10.1016/S0378-1119(01)00333-X. PMID   11250079.
6. Yogosawa S, Hatakeyama S, Nakayama KI, Miyoshi H, Kohsaka S, Akazawa C (Dec 2005). "Ubiquitylation and degradation of serum-inducible kinase by hVPS18, a RING-H2 type ubiquitin ligase". J Biol Chem. 280 (50): 41619–27. doi: 10.1074/jbc.M508397200 . PMID   16203730.
7. "Entrez Gene: VPS18 vacuolar protein sorting 18 homolog (S. cerevisiae)".
8. Kim BY, Krämer H, Yamamoto A, Kominami E, Kohsaka S, Akazawa C (August 2001). "Molecular characterization of mammalian homologues of class C Vps proteins that interact with syntaxin-7". J. Biol. Chem. 276 (31): 29393–402. doi: 10.1074/jbc.M101778200 . PMID   11382755.

Further reading

• Nagase T, Kikuno R, Ishikawa K, Hirosawa M, Ohara O (2000). "Prediction of the coding sequences of unidentified human genes. XVII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro". DNA Res. 7 (2): 143–50. doi: 10.1093/dnares/7.2.143 . PMID   10819331.
• Kim BY, Krämer H, Yamamoto A, Kominami E, Kohsaka S, Akazawa C (2001). "Molecular characterization of mammalian homologues of class C Vps proteins that interact with syntaxin-7". J. Biol. Chem. 276 (31): 29393–402. doi: 10.1074/jbc.M101778200 . PMID   11382755.
• Kim BY, Ueda M, Kominami E, Akagawa K, Kohsaka S, Akazawa C (2004). "Identification of mouse Vps16 and biochemical characterization of mammalian class C Vps complex". Biochem. Biophys. Res. Commun. 311 (3): 577–82. doi:10.1016/j.bbrc.2003.10.030. PMID   14623309.
• Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, Berriz GF, Gibbons FD, Dreze M, Ayivi-Guedehoussou N, Klitgord N, Simon C, Boxem M, Milstein S, Rosenberg J, Goldberg DS, Zhang LV, Wong SL, Franklin G, Li S, Albala JS, Lim J, Fraughton C, Llamosas E, Cevik S, Bex C, Lamesch P, Sikorski RS, Vandenhaute J, Zoghbi HY, Smolyar A, Bosak S, Sequerra R, Doucette-Stamm L, Cusick ME, Hill DE, Roth FP, Vidal M (2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–8. Bibcode:2005Natur.437.1173R. doi:10.1038/nature04209. PMID   16189514. S2CID   4427026.
• Yogosawa S, Kawasaki M, Wakatsuki S, Kominami E, Shiba Y, Nakayama K, Kohsaka S, Akazawa C (2006). "Monoubiquitylation of GGA3 by hVPS18 regulates its ubiquitin-binding ability". Biochem. Biophys. Res. Commun. 350 (1): 82–90. doi:10.1016/j.bbrc.2006.09.013. PMID   16996030.
• Olsen JV, Blagoev B, Gnad F, Macek B, Kumar C, Mortensen P, Mann M (2006). "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks". Cell. 127 (3): 635–48. doi: 10.1016/j.cell.2006.09.026 . PMID   17081983.