Virion

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Hepacivirus virion. The outer shell (capsid) of this virion consists of repeating simple faces, each built from three protein dimers Hepacivirus virion image.svg
Hepacivirus virion. The outer shell (capsid) of this virion consists of repeating simple faces, each built from three protein dimers

A virion (plural, viria or virions), is an inert virus particle capable of invading a cell. Upon entering the cell, the virion disassembles and the genetic material from the virus takes control of the cell infrastructure, thus enabling the virus to replicate. [1] The genetic material ( core , either DNA or RNA, along with occasionally present virus core protein) inside the virion is usually enclosed in a protection shell, known as the capsid. [2]

Contents

While the terms "virus" and "virion" are occasionally confused, recently "virion" is used solely to describe the virus structure outside of cells, [3] while the terms "virus/viral" are broader and also include biological properties such as the infectivity of a virion. [4]

Components

A virion consists of one or more nucleic acid genome molecules (single-stranded or double-stranded RNA or DNA) and coatings (a capsid and possibly a viral envelope). The virion may contain other proteins (for example with enzymatic activities) and/or nucleoproteins. [5]

Capsid

In the vast majority of viruses, the DNA and RNA components are packed into a protein shell, the capsid. [5] The capsid proteins are often differentiated into major and minor capsid proteins (MCP and mCP). In exceptional cases, there are also viruses without a capsid (i.e., true virions), such as the RNA viruses of the Narnaviridae and the viroids of the Pospiviroidae (with the Citrus Exocortis Viroid and the Citrus Bark Crack Viroid).

If the genome consists of several segments, these are usually packaged together in a capsid (e.g., influenza viruses), and in some viruses, the segments can also be individually packaged in their own capsids (e.g., in Nanoviridae ).

Multiple shapes of virions Capsids.jpg
Multiple shapes of virions

Since the genome of viruses is relatively simple, the capsid architecture relies on repetition of simple structures, similar to the faces of a polyhedron. Each face in turn is formed by a repetition of simpler sub-units, with the amount of repetitions called a triangulation number (T). Similar capsid structure can be used by many different types of viruses. [3]

In many viruses, the virions have icosahedral symmetry, which can be ideally isometric or elongated. Many virions also have other shapes:

From observations using microscopy, there are indications of many more distinct shapes.

Tail

Tailed bacteriophage structure: (1) head, (2) tail, (3) DNA, (4) capsid, (5) collar, (6) sheath, (7) tail fibres, (8) spikes, (9) base plate Bacteriophage structure.png
Tailed bacteriophage structure: (1) head, (2) tail, (3) DNA, (4) capsid, (5) collar, (6) sheath, (7) tail fibres, (8) spikes, (9) base plate

In some groups of viruses - such as the class Caudoviricetes ("tail viruses") and the genus Tupanvirus - the capsid carries an appendage called the "tail".

The tail of the Caudoviricetes is usually divided into:

The latter are used to establish contact with the host cell. The tail of these viruses serves as an injection device to introduce their own genome into the host cell. [6] The Caudoviricetes tail material is also differentiated into major and minor tail proteins (MTP and mTP), for example, in the Enterobacteria phage lambda. [7] In addition, there may be a tail spike protein (TSP) [8] or tail fiber protein (TFP).

Even in viruses with helical morphology (such as the Rudiviridae and Ahmunviridae ), the terminal fiber proteins responsible for the receptor binding are called tail fiber proteins (tail fiber proteins). [9] [10] [ clarification needed ]

Spikes

Spikes (peplomers) can protrude from the capsid, as in the Coronaviridae , the Tectiviridae , and others. These are used to establish contact with the host cell.

In viruses of the genus Chlorovirus , the virions have a single spike that serves as an injection device; an extendable injection apparatus are found in virions of the family Tectiviridae .

Viral envelope

In many virus species, the virion also has an outer membrane, the viral envelope. [5] The envelope includes a lipid bilayer and surface proteins, similar to the cell membranes, that are usually used for the envelope construction when the virus is exiting the cell. This structure helps with attachment to the cell and also assists evading the immune system of the host organism while the virion is searching for a cell to infect. [2]

Related Research Articles

<span class="mw-page-title-main">Capsid</span> Protein shell of a virus

A capsid is the protein shell of a virus, enclosing its genetic material. It consists of several oligomeric (repeating) structural subunits made of protein called protomers. The observable 3-dimensional morphological subunits, which may or may not correspond to individual proteins, are called capsomeres. The proteins making up the capsid are called capsid proteins or viral coat proteins (VCP). The virus genomic component inside the capsid, along with occasionally present virus core protein, is called the virus core. The capsid and core together are referred to as a nucleocapsid.

Viral evolution is a subfield of evolutionary biology and virology that is specifically concerned with the evolution of viruses. Viruses have short generation times, and many—in particular RNA viruses—have relatively high mutation rates. Although most viral mutations confer no benefit and often even prove deleterious to viruses, the rapid rate of viral mutation combined with natural selection allows viruses to quickly adapt to changes in their host environment. In addition, because viruses typically produce many copies in an infected host, mutated genes can be passed on to many offspring quickly. Although the chance of mutations and evolution can change depending on the type of virus, viruses overall have high chances for mutations.

<span class="mw-page-title-main">Lytic cycle</span> Cycle of viral reproduction

The lytic cycle is one of the two cycles of viral reproduction, the other being the lysogenic cycle. The lytic cycle results in the destruction of the infected cell and its membrane. Bacteriophages that can only go through the lytic cycle are called virulent phages.

<i>Escherichia virus T4</i> Species of bacteriophage

Escherichia virus T4 is a species of bacteriophages that infect Escherichia coli bacteria. It is a double-stranded DNA virus in the subfamily Tevenvirinae of the family Straboviridae. T4 is capable of undergoing only a lytic life cycle and not the lysogenic life cycle. The species was formerly named T-even bacteriophage, a name which also encompasses, among other strains, Enterobacteria phage T2, Enterobacteria phage T4 and Enterobacteria phage T6.

<i>Myoviridae</i> Family of viruses

Myoviridae was a family of bacteriophages in the order Caudovirales. The family Myoviridae and order Caudovirales have now been abolished, with the term myovirus now used to refer to the morphology of viruses in this former family. Bacteria and archaea serve as natural hosts. There were 625 species in this family, assigned to eight subfamilies and 217 genera.

Microviridae is a family of bacteriophages with a single-stranded DNA genome. The name of this family is derived from the ancient Greek word μικρός (mikrós), meaning "small". This refers to the size of their genomes, which are among the smallest of the DNA viruses. Enterobacteria, intracellular parasitic bacteria, and spiroplasma serve as natural hosts. There are 22 species in this family, divided among seven genera and two subfamilies.

<i>Cystovirus</i> Genus of viruses

Cystovirus is a genus of double-stranded RNA viruses which infects bacteria. It is the only genus in the family Cystoviridae. The name of the group cysto derives from Greek kystis which means bladder or sack. There are seven species in this genus.

<i>Pseudomonas virus phi6</i> Species of virus

Φ6 is the best-studied bacteriophage of the virus family Cystoviridae. It infects Pseudomonas bacteria. It has a three-part, segmented, double-stranded RNA genome, totalling ~13.5 kb in length. Φ6 and its relatives have a lipid membrane around their nucleocapsid, a rare trait among bacteriophages. It is a lytic phage, though under certain circumstances has been observed to display a delay in lysis which may be described as a "carrier state".

<span class="mw-page-title-main">T7 phage</span> Species of virus

Bacteriophage T7 is a bacteriophage, a virus that infects bacteria. It infects most strains of Escherichia coli and relies on these hosts to propagate. Bacteriophage T7 has a lytic life cycle, meaning that it destroys the cell it infects. It also possesses several properties that make it an ideal phage for experimentation: its purification and concentration have produced consistent values in chemical analyses; it can be rendered noninfectious by exposure to UV light; and it can be used in phage display to clone RNA binding proteins.

<span class="mw-page-title-main">Viral envelope</span> Outermost layer of many types of the infectious agent

A viral envelope is the outermost layer of many types of viruses. It protects the genetic material in their life cycle when traveling between host cells. Not all viruses have envelopes. A viral envelope protein or E protein is a protein in the envelope, which may be acquired by the capsid from an infected host cell.

<span class="mw-page-title-main">Viral entry</span> Earliest stage of infection in the viral life cycle

Viral entry is the earliest stage of infection in the viral life cycle, as the virus comes into contact with the host cell and introduces viral material into the cell. The major steps involved in viral entry are shown below. Despite the variation among viruses, there are several shared generalities concerning viral entry.

<i>Salmonella virus P22</i> Species of virus

Salmonella virus P22 is a bacteriophage in the Podoviridae family that infects Salmonella typhimurium. Like many phages, it has been used in molecular biology to induce mutations in cultured bacteria and to introduce foreign genetic material. P22 has been used in generalized transduction and is an important tool for investigating Salmonella genetics.

<span class="mw-page-title-main">Tectivirus</span> Family of viruses

Tectiviridae is a family of viruses with 10 species in five genera. Bacteria serve as natural hosts. Tectiviruses have no head-tail structure, but are capable of producing tail-like tubes of ~ 60×10 nm upon adsorption or after chloroform treatment. The name is derived from Latin tectus.

<i>Corticovirus</i> Genus of viruses

Corticovirus is a genus of viruses in the family Corticoviridae. Corticoviruses are bacteriophages; that is, their natural hosts are bacteria. The genus contains two species. The name is derived from Latin cortex, corticis. However, prophages closely related to PM2 are abundant in the genomes of aquatic bacteria, suggesting that the ecological importance of corticoviruses might be underestimated. Bacteriophage PM2 was first described in 1968 after isolation from seawater sampled from the coast of Chile.

<span class="mw-page-title-main">Bacteriophage Qbeta</span> Species of virus

Bacteriophage Qbeta, commonly referred to as Qbeta or Qβ, is a species consisting of several strains of positive-strand RNA virus which infects bacteria that have F-pili, most commonly Escherichia coli. Its linear genome is packaged into an icosahedral capsid with a diameter of 28 nm. Bacteriophage Qβ enters its host cell after binding to the side of the F-pilus.

<span class="mw-page-title-main">Bacteriophage P2</span> Species of virus

Bacteriophage P2, scientific name Peduovirus P2, is a temperate phage that infects E. coli. It is a tailed virus with a contractile sheath and is thus classified in the genus Peduovirus, family Peduoviridae within class Caudoviricetes. This genus of viruses includes many P2-like phages as well as the satellite phage P4.

Enquatrovirus is a genus of bacteriophages in the order Caudovirales, in the family Podoviridae. Bacteria serve as natural hosts. There is currently only one species in this genus: the type species Escherichia virus N4.

Escherichia virus CC31, formerly known as Enterobacter virus CC31, is a dsDNA bacteriophage of the subfamily Tevenvirinae responsible for infecting the bacteria family of Enterobacteriaceae. It is one of two discovered viruses of the genus Karamvirus, diverging away from the previously discovered T4virus, as a clonal complex (CC). CC31 was first isolated from Escherichia coli B strain S/6/4 and is primarily associated with Escherichia, even though is named after Enterobacter.

<i>Duplodnaviria</i> Realm of viruses

Duplodnaviria is a realm of viruses that includes all double-stranded DNA viruses that encode the HK97 fold major capsid protein. The HK97 fold major capsid protein is the primary component of the viral capsid, which stores the viral deoxyribonucleic acid (DNA). Viruses in the realm also share a number of other characteristics, such as an icosahedral capsid, an opening in the viral capsid called a portal, a protease enzyme that empties the inside of the capsid prior to DNA packaging, and a terminase enzyme that packages viral DNA into the capsid.

Bacteriophage AP205 is a plaque-forming bacteriophage that infects Acinetobacter bacteria. Bacteriophage AP205 is a protein-coated virus with a positive single-stranded RNA genome. It is a member of the family Fiersviridae, consisting of particles that infect Gram-negative bacteria such as E. coli.

References

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  2. 1 2 Reynolds & Theodore 2023, p. 20.
  3. 1 2 Reynolds & Theodore 2023, p. 24.
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  5. 1 2 3 N. J. Dimmock, Andrew J. Easton, Keith Leppard: Introduction to Modern Virology. 6th edition, Wiley & Blackwell, Malden 2007, ISBN 978-1-4051-3645-7, p. 49, Chapter 4: Classification of Viruses..
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  7. Protein Data Bank in Europe: NMR structure of the gpu tail protein from lambda bacteriophage. On: ebi.ac.uk
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  9. Laso-Pérez, Rafael; Wu, Fabai; Crémière, Antoine; Speth, Daan R.; Magyar, John S.; Zhao, Kehan; Krupovic, Mart; Orphan, Victoria J. (2023-01-19). "Evolutionary diversification of methanotrophic ANME-1 archaea and their expansive virome". Nature Microbiology. 8 (2): 231–245. doi: 10.1038/s41564-022-01297-4 . ISSN   2058-5276. PMC   9894754 . PMID   36658397.
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