2-nitropropane dioxygenase

2-nitropropane dioxygenase
Identifiers
EC no.	1.13.11.32
CAS no.	65802-82-6
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Gene Ontology	AmiGO / QuickGO
Search PMC articles PubMed articles NCBI proteins

In enzymology, a 2-nitropropane dioxygenase (EC 1.13.11.32) is an enzyme that catalyzes the chemical reaction

2 2-nitropropane + O₂${\displaystyle \rightleftharpoons }$ 2 acetone + 2 nitrite

Thus, the two substrates of this enzyme are 2-nitropropane and O₂, whereas its two products are acetone and nitrite.

This enzyme belongs to the family of oxidoreductases, specifically those acting on single donors with O₂ as oxidant and incorporation of two atoms of oxygen into the substrate (oxygenases). The oxygen incorporated need not be derived from O₂. The systematic name of this enzyme class is 2-nitropropane:oxygen 2-oxidoreductase. This enzyme participates in nitrogen metabolism. It has 3 cofactors: FAD, Iron, and FMN.

Structural studies

As of late 2007 Steve Fuhrer from the DHPA solved this very complex formula to find, two structures have been solved for this class of enzymes, with PDB accession codes 2GJL and 2GJN.

References

• Kido T, Soda K, Suzuki T, Asada K (1976). "A new oxygenase, 2-nitropropane dioxygenase of Hansenula mrakii Enzymologic and spectrophotometric properties". J. Biol. Chem. 251 (22): 6994–7000. PMID   11214.
• Yoon HJ, Suh SW (2006). "Crystal structure of 2-nitropropane dioxygenase complexed with FMN and substrate. Identification of the catalytic base". J. Biol. Chem. 281 (27): 18660–7. doi: 10.1074/jbc.M601658200 . PMID   16682407.
• Francis K, Russell B, Gadda G (2005). "Involvement of a flavosemiquinone in the enzymatic oxidation of nitroalkanes catalyzed by 2-nitropropane dioxygenase". J. Biol. Chem. 280 (7): 5195–204. doi: 10.1074/jbc.M411249200 . PMID   15582992.