| Acholeplasma laidlawii | |
|---|---|
Scientific classification  | |
| Domain: | Bacteria |
| Phylum: | Mycoplasmatota |
| Class: | Mollicutes |
| Order: | Acholeplasmatales |
| Family: | Acholeplasmataceae |
| Genus: | Acholeplasma |
| Species: | A. laidlawii |
| Binomial name | |
| Acholeplasma laidlawii (Sabin 1941) Edward and Freundt 1970 [1] | |
Acholeplasma laidlawii are small bacteria which lack a cell wall. [2] Like other Acholeplasma and Mycoplasma, A. laidlawii has been identified as a common contaminant of growth media for cell culture. [3]
A. laidlawii was first isolated from sewage in London in 1936 and was named after its discoverer, Patrick Laidlaw. [4]
A. laidlawii has a relatively small genome comprising 1.5Mbp. Additionally its genome has a low GC-content of just 31%. [2] The A. laidlawii genome has been sequenced. [2]
Acholeplasma laidlawii may contaminate bovine serum and also occurs in serum-free cell culture media products. The presence of A. laidlawii in broth powders is a serious problem in routine biopharmaceutical operations where filtration is used as a sterilisation procedure. A. laidlawii may flourish and survive for prolonged periods at refrigeration and ambient temperatures in serum-free cell culture media.
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Escherichia coli ( ESH-ə-RIK-ee-ə KOH-ly) is a Gram-negative, facultative anaerobic, rod-shaped, coliform bacterium of the genus Escherichia that is commonly found in the lower intestine of warm-blooded organisms. Most E. coli strains are harmless, but some serotypes such as EPEC, and ETEC are pathogenic and can cause serious food poisoning in their hosts, and are occasionally responsible for food contamination incidents that prompt product recalls. Most strains are part of the normal microbiota of the gut and are harmless or even beneficial to humans (although these strains tend to be less studied than the pathogenic ones). For example, some strains of E. coli benefit their hosts by producing vitamin K2 or by preventing the colonization of the intestine by pathogenic bacteria. These mutually beneficial relationships between E. coli and humans are a type of mutualistic biological relationship — where both the humans and the E. coli are benefitting each other. E. coli is expelled into the environment within fecal matter. The bacterium grows massively in fresh fecal matter under aerobic conditions for three days, but its numbers decline slowly afterwards.
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Acholeplasmataceae is a family of bacteria. It is the only family in the order Acholeplasmatales, placed in the class Mollicutes. The family comprises the genera Acholeplasma and Phytoplasma. Phytoplasma has the candidatus status, because members still could not be cultured.
Acholeplasma are wall-less bacteria in the Mollicutes class. They include saprotrophic or pathogenic species. There are 15 recognised species. The G+C content is low, ranging from 26 - 36% (mol%). The genomes of Acholeplasma species range in size from 1.5 to 1.65 Mbp. Cholesterol is not required for growth. The species are found on animals, and some plants and insects. The optimum growth temperature is 30 to 37 degrees Celsius.
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