CIMAP1C

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CIMAP1C (ciliary microtubule associated protein 1C) is a gene in humans that encodes the CIMAP1C protein. It is also often referred to as ODF3L1 (outer dense fiber protein 3-like protein 1). CIMAP1C is expressed in low levels throughout the body with high expression levels in the testes. It is highly conserved in mammals and reptiles but not present in birds or amphibians, indicating it arose around 300 million years ago.

Contents

Gene

The CIMAP1C gene is found on Chromosome 15 at position 15q24.2, spanning 3.72 kb. [1] It contains 4 exons and 1,132 nucleotides. [2]

Aliases

CIMAP1C is also known as ODF3L1 (outer dense fiber protein 3 like protein 1). [2]

Protein

CIMAP1C tertiary structure created by I-Tasser. Coloring is rainbow from left to right, where red represents the N-terminus and purple the C-terminus. I-Tasser CIMAP1C Tertiary Structure.png
CIMAP1C tertiary structure created by I-Tasser. Coloring is rainbow from left to right, where red represents the N-terminus and purple the C-terminus.

The CIMAP1C protein is 274 amino acids long and its molecular weight is ~31 kDa. [4] Its isoelectric point is 9.6. [5] There are no known isoforms. [2] CIMAP1C is very basic, proline rich, and highly tyrosine rich. [6] There are two structurally significant regions known as sperm tail proline-glycine rich repeat regions. [2] These regions are highly conserved and contain glycosylation and phosphorylation sites. [7]

Gene level regulation

CIMAP1C is expressed at low levels in most tissues in the body (15.7%), with high expression in the testis. [2] Slightly high levels of expression are seen in the mammary glands. [2]

Protein level regulation

CIMAP1C is imported into the nucleus via two nuclear localization signals, and can otherwise be found in the cytoskeleton. [2] [8] CIMAP1C contains a variety of phosphorylation sites, specifically protein kinase C and Casein kinase II phosphorylation. [7]

Evolution

Paralogs

There are no paralogs of the CIMAP1C gene. [2]

Corrected sequence divergence of the CIMAP1C, Cytochrome C and Fibrinogen alpha divergence dates. The CIMAP1C and Fibrinogen alpha dates are in orange and yellow respectively and are on top of one another. CIMAP1C Protein Divergence Over Time fixed.png
Corrected sequence divergence of the CIMAP1C, Cytochrome C and Fibrinogen alpha divergence dates. The CIMAP1C and Fibrinogen alpha dates are in orange and yellow respectively and are on top of one another.

Orthologs

CIMAP1C is highly conserved, and can be found in mammals and reptiles. CIMAP1C is not found in amphibians or birds, therefore it arose 300 million years ago. [9] Based on the calculated protein divergence figure, CIMAP1C evolves rapidly and similarly to Fibrinogen Alpha. The table below lists orthologs and their information.

CIMAP1C Orthologs
Genus and SpeciesCommon NameTaxonomic GroupMediat Date of Divergence (MYA) [9] Accession numberSequence length (aa)Sequence Identity to human protein (%)Sequence Similarity to human Protein (%)
Homo sapiens Human Primates 0.0NP_787077.1274100.0100.0
Saimiri boliviensis Squirrel MonkeyPrimates43XP_010329148.127487.692.7
Canis lupus familiaris Dog Carnivora 94XP_038297715.127578.288.4
Mus musculus House Mouse Rodentia 87NP_001361610.130664.475.5
Myotis brandtii Brandt's Bat Chiroptera 94XP_005884580.127574.585.8
Gracilinanus agilis Mouse Opossum Didelphimorphia 160XP_044518760.127858.873.8
Bubalus bubalis Water buffalo Artiodactyla 94XP_006080752.327473.184.4
Pogona vitticeps Central Bearded Dragon Squamata 319XP_020640486.128540.254.3
Notechis scutatus Tiger SnakeSquamata319XP_026540911.119528.441.4
Dermochelys coriacea Leatherback Sea Turtle Testudines 319XP_038274105.127437.854.7
Alligator mississippiensis American Alligator Crocodylia 319XP_019333216.124937.852.8
Phascolarctos cinereus Koala Diprotodontia 160XP_020850090.12785973.4
Phocoena sinus VaquitaArtiodactyla94XP_032479987.127577.587.3
Eublepharis macularius Leopard geckoSquamata319XP_054858185.127638.550.7
Ornithorhynchus anatinus Platypus Monotremata 180XP_001509549.126945.858.8

Interacting proteins

It has been experimentally determined that CIMAP1C interacts with STAMBP (STAM binding protein) through affinity chromatography. [10] STAMBP regulates cell surface receptor-mediated endocytosis and ubiquitin-dependent receptor sorting to lysosomes. [11]

Clinical significance

Expression levels of CIMAP1C are positive markers of disease prognosis for those with papillary thyroid carcinoma (PTC) and non-metastatic breast cancer (NMBC). High levels of CIMAP1C in tumor tissues accurately predicted a better prognosis for individuals with PTC. [12] CIMAP1C is also noted as a positive indicator for the effectiveness of NMBC radiotherapy. [13]

References

  1. ↑ "AceView: a comprehensive cDNA-supported gene and transcripts annotation". 11 June 2023.
  2. 1 2 3 4 5 6 7 8 "CIMAP1C ciliary microtubule associated protein 1C [Homo sapiens (human)] - Gene - NCBI". www.ncbi.nlm.nih.gov. Retrieved 2023-07-28.
  3. ↑ Zhang, Yang (2009). "I-TASSER: Fully automated protein structure prediction in CASP8". Proteins: Structure, Function, and Bioinformatics. 77 (S9): 100–113. doi:10.1002/prot.22588. ISSN   0887-3585. PMC   2782770 . PMID   19768687.
  4. ↑ "Thermo Fisher ODF3L1 Antibodies - polyclonal rabbit anti-human ODF3L1 antibody". 9 July 2023.
  5. ↑ Duvaud, Séverine; Gabella, Chiara; Lisacek, Frédérique; Stockinger, Heinz; Ioannidis, Vassilios; Durinx, Christine (2021-04-13). "Expasy, the Swiss Bioinformatics Resource Portal, as designed by its users". Nucleic Acids Research. 49 (W1): W216 –W227. doi:10.1093/nar/gkab225. ISSN   0305-1048. PMC   8265094 . PMID   33849055.
  6. ↑ "EMBL-EBI Tools: An introduction". EMBL's European Bioinformatics Institute. doi:10.6019/tol.ebitools-t.2021.00001.1.{{cite web}}: Missing or empty |url= (help)
  7. 1 2 Blom, Nikolaj; Gammeltoft, Steen; Brunak, Søren (December 1999). "Sequence and structure-based prediction of eukaryotic protein phosphorylation sites". Journal of Molecular Biology. 294 (5): 1351–1362. doi:10.1006/jmbi.1999.3310. ISSN   0022-2836. PMID   10600390.
  8. ↑ Thumuluri, Vineet; Almagro Armenteros, José Juan; Rosenberg Johansen, Alexander; Nielsen, Henrik; Winther, Ole (2022-04-30). "DeepLoc 2.0: multi-label subcellular localization prediction using protein language models". Nucleic Acids Research. 50 (W1): W228 –W234. doi:10.1093/nar/gkac278. ISSN   0305-1048. PMC   9252801 . PMID   35489069.
  9. 1 2 "Data S1: RaxML file, TimeTree, and alignments". doi: 10.7717/peerj.5401/supp-3 .{{cite web}}: Missing or empty |url= (help)
  10. ↑ "ODF3L1 protein (human) - STRING interaction network". string-db.org. Retrieved 2023-07-28.
  11. ↑ Huttlin, Edward L.; Bruckner, Raphael J.; Navarrete-Perea, Jose; Cannon, Joe R.; Baltier, Kurt; Gebreab, Fana; Gygi, Melanie P.; Thornock, Alexandra; Zarraga, Gabriela; Tam, Stanley; Szpyt, John; Gassaway, Brandon M.; Panov, Alexandra; Parzen, Hannah; Fu, Sipei (May 2021). "Dual proteome-scale networks reveal cell-specific remodeling of the human interactome". Cell. 184 (11): 3022–3040.e28. doi:10.1016/j.cell.2021.04.011. ISSN   0092-8674. PMC   8165030 . PMID   33961781.
  12. ↑ Guo, Mengli; Chen, Zhen; Li, Yayi; Li, Sijin; Shen, Fei; Gan, Xiaoxiong; Feng, Jianhua; Cai, Wensong; Liu, Qingzhi; Xu, Bo (2021-06-23). "Tumor Mutation Burden Predicts Relapse in Papillary Thyroid Carcinoma With Changes in Genes and Immune Microenvironment". Frontiers in Endocrinology. 12 674616. doi: 10.3389/fendo.2021.674616 . ISSN   1664-2392. PMC   8261145 . PMID   34248843.
  13. ↑ Chen, Huajian; Huang, Li; Wan, Xinlong; Ren, Shigang; Chen, Haibin; Ma, Shumei; Liu, Xiaodong (March 2023). "Polygenic risk score for prediction of radiotherapy efficacy and radiosensitivity in patients with non-metastatic breast cancer". Radiation Medicine and Protection. 4 (1): 33–42. doi: 10.1016/j.radmp.2023.01.001 .
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