CYTH1

CYTH1
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 1BC9, 4A4P
Identifiers
Aliases	CYTH1 , B2-1, CYTOHESIN-1, D17S811E, PSCD1, SEC7, cytohesin 1
External IDs	OMIM: 182115 MGI: 1334257 HomoloGene: 31262 GeneCards: CYTH1
Gene location (Human) Chr. Chromosome 17 (human) [1] Band 17q25.3 Start 78,674,048 bp [1] End 78,782,297 bp [1]
Gene location (Mouse) Chr. Chromosome 11 (mouse) [2] Band 11|11 E2 Start 118,022,845 bp [2] End 118,139,418 bp [2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in blood monocyte corpus callosum lymph node bone marrow cells spleen appendix middle frontal gyrus thymus sural nerve Top expressed in motor neuron barrel cortex submandibular gland cerebellar cortex nucleus accumbens intercostal muscle digastric muscle sternocleidomastoid muscle Paneth cell lip More reference expression data BioGPS More reference expression data
Gene ontology Molecular function protein binding guanyl-nucleotide exchange factor activity lipid binding Cellular component cytoplasm extrinsic component of cytoplasmic side of plasma membrane plasma membrane membrane cytosol Golgi membrane adherens junction bicellular tight junction cell junction Biological process regulation of ARF protein signal transduction regulation of cell adhesion vesicle-mediated transport establishment of epithelial cell polarity Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 9267 19157 Ensembl ENSG00000108669 ENSMUSG00000017132 UniProt Q15438 Q9QX11 RefSeq (mRNA) NM_001292018 NM_001292019 NM_004762 NM_017456 NM_001365037 NM_001365038 NM_001365039 NM_001365040 NM_001365041 NM_001394676 NM_001394677 NM_001394678 NM_001112699 NM_001112700 NM_011180 NM_001378885 NM_001378886 NM_001378887 NM_001378888 RefSeq (protein) NP_001278947 NP_001278948 NP_004753 NP_059430 NP_001351966 NP_001351967 NP_001351968 NP_001351969 NP_001351970 NP_001106169 NP_001106170 NP_035310 NP_001365814 NP_001365815 NP_001365816 NP_001365817 Location (UCSC) Chr 17: 78.67 – 78.78 Mb Chr 11: 118.02 – 118.14 Mb PubMed search [3] [4]
Wikidata
View/Edit Human View/Edit Mouse

Cytohesin-1 formerly known as Pleckstrin homology, Sec7 and coiled/coil domains 1 (PSCD1) is a protein that in humans is encoded by the CYTH1 gene. ^{[5] [6] [7]}

Function

Cytohesin-1 (CYTH1) is a member of the cytohesin family. Members of this family have identical structural organization that consists of an N-terminal coiled-coil motif, a central Sec7 domain, and a C-terminal pleckstrin homology (PH) domain. The coiled-coil motif is involved in homodimerization, the Sec7 domain contains guanine-nucleotide exchange protein (GEP) activity, and the PH domain interacts with phospholipids and is responsible for association of CYTHs with membranes. Members of this family appear to mediate the regulation of protein sorting and membrane trafficking. The CYTH1 is highly expressed in natural killer and peripheral T cells, and regulates the adhesiveness of integrins at the plasma membrane of lymphocytes. CYTH1 protein is 83% homologous to CYTH2. ^[7]

Interactions

CYTH1 has been shown to interact with:

• ARFRP1, ^[8]
• CD18, ^{[9] [10]} and
• TRIM23. ^[11]

References

1. GRCh38: Ensembl release 89: ENSG00000108669 - Ensembl, May 2017
2. GRCm38: Ensembl release 89: ENSMUSG00000017132 - Ensembl, May 2017
3. "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
4. "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
5. Liu L, Pohajdak B (September 1992). "Cloning and sequencing of a human cDNA from cytolytic NK/T cells with homology to yeast SEC7". Biochim. Biophys. Acta. 1132 (1): 75–8. doi:10.1016/0167-4781(92)90055-5. PMID   1511013.
6. Dixon B, Mansour M, Pohajdak B (April 1993). "Assignment of human B2-1 gene (D17S811E) to chromosome 17qter by PCR analysis of somatic cell hybrids and fluorescence in situ hybridization". Cytogenet. Cell Genet. 63 (1): 42–4. doi:10.1159/000133498. PMID   8449036.
7. "Entrez Gene: PSCD1 pleckstrin homology, Sec7 and coiled-coil domains 1(cytohesin 1)".
8. Schürmann A, Schmidt M, Asmus M, Bayer S, Fliegert F, Koling S, Massmann S, Schilf C, Subauste MC, Voss M, Jakobs KH, Joost HG (April 1999). "The ADP-ribosylation factor (ARF)-related GTPase ARF-related protein binds to the ARF-specific guanine nucleotide exchange factor cytohesin and inhibits the ARF-dependent activation of phospholipase D". J. Biol. Chem. 274 (14): 9744–51. doi: 10.1074/jbc.274.14.9744 . PMID   10092663.
9. Rietzler M, Bittner M, Kolanus W, Schuster A, Holzmann B (October 1998). "The human WD repeat protein WAIT-1 specifically interacts with the cytoplasmic tails of beta7-integrins". J. Biol. Chem. 273 (42): 27459–66. doi: 10.1074/jbc.273.42.27459 . PMID   9765275.
10. Geiger C, Nagel W, Boehm T, van Kooyk Y, Figdor CG, Kremmer E, Hogg N, Zeitlmann L, Dierks H, Weber KS, Kolanus W (June 2000). "Cytohesin-1 regulates beta-2 integrin-mediated adhesion through both ARF-GEF function and interaction with LFA-1". EMBO J. 19 (11): 2525–36. doi:10.1093/emboj/19.11.2525. PMC   212768 . PMID   10835351.
11. Vitale N, Pacheco-Rodriguez G, Ferrans VJ, Riemenschneider W, Moss J, Vaughan M (July 2000). "Specific functional interaction of human cytohesin-1 and ADP-ribosylation factor domain protein (ARD1)". J. Biol. Chem. 275 (28): 21331–9. doi: 10.1074/jbc.M909642199 . PMID   10748148.

Further reading

• Andersson B, Wentland MA, Ricafrente JY, Liu W, Gibbs RA (1996). "A "double adaptor" method for improved shotgun library construction". Anal. Biochem. 236 (1): 107–13. doi:10.1006/abio.1996.0138. PMID   8619474.
• Kolanus W, Nagel W, Schiller B, Zeitlmann L, Godar S, Stockinger H, Seed B (1996). "Alpha L beta 2 integrin/LFA-1 binding to ICAM-1 induced by cytohesin-1, a cytoplasmic regulatory molecule". Cell. 86 (2): 233–42. doi: 10.1016/S0092-8674(00)80095-1 . PMID   8706128. S2CID   18390795.
• Meacci E, Tsai SC, Adamik R, Moss J, Vaughan M (1997). "Cytohesin-1, a cytosolic guanine nucleotide-exchange protein for ADP-ribosylation factor". Proc. Natl. Acad. Sci. U.S.A. 94 (5): 1745–8. Bibcode:1997PNAS...94.1745M. doi: 10.1073/pnas.94.5.1745 . PMC   19987 . PMID   9050849.
• Yu W, Andersson B, Worley KC, Muzny DM, Ding Y, Liu W, Ricafrente JY, Wentland MA, Lennon G, Gibbs RA (1997). "Large-scale concatenation cDNA sequencing". Genome Res. 7 (4): 353–8. doi:10.1101/gr.7.4.353. PMC   139146 . PMID   9110174.
• Nagel W, Zeitlmann L, Schilcher P, Geiger C, Kolanus J, Kolanus W (1998). "Phosphoinositide 3-OH kinase activates the beta2 integrin adhesion pathway and induces membrane recruitment of cytohesin-1". J. Biol. Chem. 273 (24): 14853–61. doi: 10.1074/jbc.273.24.14853 . PMID   9614087.
• Betz SF, Schnuchel A, Wang H, Olejniczak ET, Meadows RP, Lipsky BP, Harris EA, Staunton DE, Fesik SW (1998). "Solution structure of the cytohesin-1 (B2-1) Sec7 domain and its interaction with the GTPase ADP ribosylation factor 1". Proc. Natl. Acad. Sci. U.S.A. 95 (14): 7909–14. Bibcode:1998PNAS...95.7909B. doi: 10.1073/pnas.95.14.7909 . PMC   20903 . PMID   9653114.
• Nagel W, Schilcher P, Zeitlmann L, Kolanus W (1998). "The PH domain and the polybasic c domain of cytohesin-1 cooperate specifically in plasma membrane association and cellular function". Mol. Biol. Cell. 9 (8): 1981–94. doi:10.1091/mbc.9.8.1981. PMC   25450 . PMID   9693361.
• Pacheco-Rodriguez G, Meacci E, Vitale N, Moss J, Vaughan M (1998). "Guanine nucleotide exchange on ADP-ribosylation factors catalyzed by cytohesin-1 and its Sec7 domain". J. Biol. Chem. 273 (41): 26543–8. doi: 10.1074/jbc.273.41.26543 . PMID   9756891.
• Rietzler M, Bittner M, Kolanus W, Schuster A, Holzmann B (1998). "The human WD repeat protein WAIT-1 specifically interacts with the cytoplasmic tails of beta7-integrins". J. Biol. Chem. 273 (42): 27459–66. doi: 10.1074/jbc.273.42.27459 . PMID   9765275.
• Schürmann A, Schmidt M, Asmus M, Bayer S, Fliegert F, Koling S, Massmann S, Schilf C, Subauste MC, Voss M, Jakobs KH, Joost HG (1999). "The ADP-ribosylation factor (ARF)-related GTPase ARF-related protein binds to the ARF-specific guanine nucleotide exchange factor cytohesin and inhibits the ARF-dependent activation of phospholipase D". J. Biol. Chem. 274 (14): 9744–51. doi: 10.1074/jbc.274.14.9744 . PMID   10092663.
• Pacheco-Rodriguez G, Patton WA, Adamik R, Yoo HS, Lee FJ, Zhang GF, Moss J, Vaughan M (1999). "Structural elements of ADP-ribosylation factor 1 required for functional interaction with cytohesin-1". J. Biol. Chem. 274 (18): 12438–44. doi: 10.1074/jbc.274.18.12438 . PMID   10212218.
• Venkateswarlu K, Gunn-Moore F, Tavaré JM, Cullen PJ (1999). "EGF-and NGF-stimulated translocation of cytohesin-1 to the plasma membrane of PC12 cells requires PI 3-kinase activation and a functional cytohesin-1 PH domain". J. Cell Sci. 112 ( Pt 12) (12): 1957–65. doi:10.1242/jcs.112.12.1957. PMID   10341214.
• Ogasawara M, Kim SC, Adamik R, Togawa A, Ferrans VJ, Takeda K, Kirby M, Moss J, Vaughan M (2000). "Similarities in function and gene structure of cytohesin-4 and cytohesin-1, guanine nucleotide-exchange proteins for ADP-ribosylation factors". J. Biol. Chem. 275 (5): 3221–30. doi: 10.1074/jbc.275.5.3221 . PMID   10652308.
• Vitale N, Pacheco-Rodriguez G, Ferrans VJ, Riemenschneider W, Moss J, Vaughan M (2000). "Specific functional interaction of human cytohesin-1 and ADP-ribosylation factor domain protein (ARD1)". J. Biol. Chem. 275 (28): 21331–9. doi: 10.1074/jbc.M909642199 . PMID   10748148.
• Lee SY, Mansour M, Pohajdak B (2000). "B2-1, a Sec7- and pleckstrin homology domain-containing protein, localizes to the Golgi complex". Exp. Cell Res. 256 (2): 515–21. doi:10.1006/excr.2000.4845. PMID   10772823.
• Geiger C, Nagel W, Boehm T, van Kooyk Y, Figdor CG, Kremmer E, Hogg N, Zeitlmann L, Dierks H, Weber KS, Kolanus W (2000). "Cytohesin-1 regulates beta-2 integrin-mediated adhesion through both ARF-GEF function and interaction with LFA-1". EMBO J. 19 (11): 2525–36. doi:10.1093/emboj/19.11.2525. PMC   212768 . PMID   10835351.
• Dierks H, Kolanus J, Kolanus W (2001). "Actin cytoskeletal association of cytohesin-1 is regulated by specific phosphorylation of its carboxyl-terminal polybasic domain". J. Biol. Chem. 276 (40): 37472–81. doi: 10.1074/jbc.M101502200 . PMID   11438522.
• Tang P, Cheng TP, Agnello D, Wu CY, Hissong BD, Watford WT, Ahn HJ, Galon J, Moss J, Vaughan M, O'Shea JJ, Gadina M (2002). "Cybr, a cytokine-inducible protein that binds cytohesin-1 and regulates its activity". Proc. Natl. Acad. Sci. U.S.A. 99 (5): 2625–9. Bibcode:2002PNAS...99.2625T. doi: 10.1073/pnas.052712999 . PMC   122398 . PMID   11867758.